first strand synthesis reaction buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs first strand synthesis reaction buffer
    First Strand Synthesis Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand synthesis reaction buffer/product/New England Biolabs
    Average 99 stars, based on 74 article reviews
    Price from $9.99 to $1999.99
    first strand synthesis reaction buffer - by Bioz Stars, 2020-04
    99/100 stars

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    RNA Extraction:

    Article Title: The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses.
    Article Snippet: Library preparation and examination for sequencing Total RNA from tissues was extracted using MiniBEST Universal RNA Extraction Kit (Takara, Shiga, Japan) following the manufacturer's recommendations. .. The sequencing libraries were constructed with varied index labels by a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, USA) , and the details are as follows: first, ribosomal RNA was removed utilizing Epicentre Ribo-Zero™ Gold Kits (Epicentre, USA), and then NEBNext First-Strand Synthesis Reaction Buffer under raised temperature was used to fracture RNA into short RNA strands.

    Sequencing:

    Article Title: The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses.
    Article Snippet: .. The sequencing libraries were constructed with varied index labels by a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, USA) , and the details are as follows: first, ribosomal RNA was removed utilizing Epicentre Ribo-Zero™ Gold Kits (Epicentre, USA), and then NEBNext First-Strand Synthesis Reaction Buffer under raised temperature was used to fracture RNA into short RNA strands. .. Subsequently, random hexamer primers were used to synthesize the firststrand cDNA by utilizing RNA fragments as a template.

    Ligation:

    Article Title: The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses.
    Article Snippet: The sequencing libraries were constructed with varied index labels by a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, USA) , and the details are as follows: first, ribosomal RNA was removed utilizing Epicentre Ribo-Zero™ Gold Kits (Epicentre, USA), and then NEBNext First-Strand Synthesis Reaction Buffer under raised temperature was used to fracture RNA into short RNA strands. .. Later on, terminal repair, poly(A) tailing and adapter ligation were implemented.

    Spectrophotometry:

    Article Title: The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses.
    Article Snippet: RNA degradation and purity were detected using 1% agarose gels and kaiaoK5500® spectrophotometer (Kaiao, Beijing, China), respectively. .. The sequencing libraries were constructed with varied index labels by a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, USA) , and the details are as follows: first, ribosomal RNA was removed utilizing Epicentre Ribo-Zero™ Gold Kits (Epicentre, USA), and then NEBNext First-Strand Synthesis Reaction Buffer under raised temperature was used to fracture RNA into short RNA strands.

    Purification:

    Article Title: The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses.
    Article Snippet: The sequencing libraries were constructed with varied index labels by a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, USA) , and the details are as follows: first, ribosomal RNA was removed utilizing Epicentre Ribo-Zero™ Gold Kits (Epicentre, USA), and then NEBNext First-Strand Synthesis Reaction Buffer under raised temperature was used to fracture RNA into short RNA strands. .. Afterwards, second-strand cDNA synthesis was performed using a buffer, dNTPs, DNA polymerase I and RNase H. Then the library fragments were purified with QIAQuick PCR kits, and eluted with elution buffer (EB).

    Concentration Assay:

    Article Title: The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses.
    Article Snippet: The RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) was used for RNA integrity and concentration assessment. .. The sequencing libraries were constructed with varied index labels by a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, USA) , and the details are as follows: first, ribosomal RNA was removed utilizing Epicentre Ribo-Zero™ Gold Kits (Epicentre, USA), and then NEBNext First-Strand Synthesis Reaction Buffer under raised temperature was used to fracture RNA into short RNA strands.

    Random Hexamer Labeling:

    Article Title: The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses.
    Article Snippet: The sequencing libraries were constructed with varied index labels by a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, USA) , and the details are as follows: first, ribosomal RNA was removed utilizing Epicentre Ribo-Zero™ Gold Kits (Epicentre, USA), and then NEBNext First-Strand Synthesis Reaction Buffer under raised temperature was used to fracture RNA into short RNA strands. .. Subsequently, random hexamer primers were used to synthesize the firststrand cDNA by utilizing RNA fragments as a template.

    Construct:

    Article Title: The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses.
    Article Snippet: .. The sequencing libraries were constructed with varied index labels by a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, USA) , and the details are as follows: first, ribosomal RNA was removed utilizing Epicentre Ribo-Zero™ Gold Kits (Epicentre, USA), and then NEBNext First-Strand Synthesis Reaction Buffer under raised temperature was used to fracture RNA into short RNA strands. .. Subsequently, random hexamer primers were used to synthesize the firststrand cDNA by utilizing RNA fragments as a template.

    Polymerase Chain Reaction:

    Article Title: The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses.
    Article Snippet: The sequencing libraries were constructed with varied index labels by a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, USA) , and the details are as follows: first, ribosomal RNA was removed utilizing Epicentre Ribo-Zero™ Gold Kits (Epicentre, USA), and then NEBNext First-Strand Synthesis Reaction Buffer under raised temperature was used to fracture RNA into short RNA strands. .. Afterwards, second-strand cDNA synthesis was performed using a buffer, dNTPs, DNA polymerase I and RNase H. Then the library fragments were purified with QIAQuick PCR kits, and eluted with elution buffer (EB).

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  • 99
    New England Biolabs first strand synthesis reaction buffer
    First Strand Synthesis Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand synthesis reaction buffer/product/New England Biolabs
    Average 99 stars, based on 74 article reviews
    Price from $9.99 to $1999.99
    first strand synthesis reaction buffer - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    New England Biolabs 5x nebnext first strand synthesis reaction buffer
    5x Nebnext First Strand Synthesis Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5x nebnext first strand synthesis reaction buffer/product/New England Biolabs
    Average 96 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    5x nebnext first strand synthesis reaction buffer - by Bioz Stars, 2020-04
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