first dimension tle buffer  (Millipore)


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    Formic acid
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    Catalog Number:
    27002
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    Structured Review

    Millipore first dimension tle buffer
    Formic acid

    https://www.bioz.com/result/first dimension tle buffer/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    first dimension tle buffer - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: VMAT2 and dopamine neuron loss in a primate model of Parkinson’s disease
    Article Snippet: .. Sections were incubated with antibodies against Glial fibrillary acidic protein (1 : 5000, rabbit, Dako, Carprinteria, CA, USA), LN3 antibody (1 : 500, mouse, Novocastra, Newcastle, UK), tyrosine hydroxylase (TH) (1 : 2000, rabbit, Chemicon, Temecula, CA, USA), nitrotyrosine (NT; 1 : 500, rabbit, Chemicon), and α-synuclein (1 : 5000, Chemicon; with or without pre-treatment with 20% formic acid) at 4°C for 48 h. After rinsing in TBS, sections were incubated with biotinylated secondary antibodies (1 : 250, Vector, Burlingame, CA, USA) for 60 min and the avidin–biotin peroxidase complex solution (1 : 1600, ABC Elite, Vector) for 60 min at 22–24°C. ..

    Light Microscopy:

    Article Title: Evaluation of Posterolateral Lumbar Fusion in Sheep Using Mineral Scaffolds Seeded with Cultured Bone Marrow Cells
    Article Snippet: .. Next, sections were either stained with toluidine blue and pyronin G (using an etching pre-treatment with 2% formic acid), or with the von Kossa stain for a light microscopy study (all strainers from Sigma–Aldrich, Spain). .. Histomorphometry Stained sections were digitized using an optical scanner (HP Scanjet 8200, Hewlett-Packard, Palo Alto, CA, USA) at a 4800-dpi resolution (pixel size = 5.2 µm) [ ].

    Incubation:

    Article Title: VMAT2 and dopamine neuron loss in a primate model of Parkinson’s disease
    Article Snippet: .. Sections were incubated with antibodies against Glial fibrillary acidic protein (1 : 5000, rabbit, Dako, Carprinteria, CA, USA), LN3 antibody (1 : 500, mouse, Novocastra, Newcastle, UK), tyrosine hydroxylase (TH) (1 : 2000, rabbit, Chemicon, Temecula, CA, USA), nitrotyrosine (NT; 1 : 500, rabbit, Chemicon), and α-synuclein (1 : 5000, Chemicon; with or without pre-treatment with 20% formic acid) at 4°C for 48 h. After rinsing in TBS, sections were incubated with biotinylated secondary antibodies (1 : 250, Vector, Burlingame, CA, USA) for 60 min and the avidin–biotin peroxidase complex solution (1 : 1600, ABC Elite, Vector) for 60 min at 22–24°C. ..

    Article Title: Evaluation of the utility of neutral-loss-dependent MS3 strategies in large-scale phosphorylation analysis
    Article Snippet: .. For IMAC phosphopeptide enrichment, desalted phosphopeptides were dissolved in 120 µL of IMAC-binding buffer [40% ACN, 25 mM formic acid (FA)] and incubated for 60 min with 10 µL PhosSelect IMAC resin (Sigma, St. Louis, MO) previously equilibrated with the same buffer. ..

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Comparative In Vitro Assessment of a Range of Commercial Feed Additives with Multiple Mycotoxin Binding Claims
    Article Snippet: .. Chemicals and Reagents Hydrochloric acid (HCl), citric acid, monobasic sodium phosphate (NaH2 PO4 ), pancreatin, pepsin, polytetrafluoroethylene (PTFE) filter, formic acid, bile salt, sodium chloride (NaCl), sodium bicarbonate (NaHCO3 ), LC-MS grade methanol and acetonitrile were supplied by Sigma-Aldrich (Gillingham, UK). .. Mycotoxins—AFB1, ZEN, FB1, OTA, T-2 and DON—crystalline solids were obtained from Romer Labs GmbH (Tulln, Austria).

    Staining:

    Article Title: Evaluation of Posterolateral Lumbar Fusion in Sheep Using Mineral Scaffolds Seeded with Cultured Bone Marrow Cells
    Article Snippet: .. Next, sections were either stained with toluidine blue and pyronin G (using an etching pre-treatment with 2% formic acid), or with the von Kossa stain for a light microscopy study (all strainers from Sigma–Aldrich, Spain). .. Histomorphometry Stained sections were digitized using an optical scanner (HP Scanjet 8200, Hewlett-Packard, Palo Alto, CA, USA) at a 4800-dpi resolution (pixel size = 5.2 µm) [ ].

    Binding Assay:

    Article Title: Galantamine-induced Amyloid-? Clearance Mediated via Stimulation of Microglial Nicotinic Acetylcholine Receptors *
    Article Snippet: .. 4 The abbreviations used are: AD Alzheimer disease Aβ amyloid-β Aβ42 Aβ1–42 AChE acetylcholinesterase α7 nAChR α7 subunit of nAChR APL allosterically potentiating ligand Ca2+ (−) DMEM Ca2+ -free DMEM Ca2+ (+) DMEM Ca2+ -free DMEM supplemented with 1.8 m m Ca2+ CaM calmodulin CaMKII Ca2+ /CaM-dependent protein kinase II choline (−) choline-free choline (+) DMEM choline (−) DMEM supplemented with 28 μ m choline FA formic acid FSB 1-fluoro-2,5-bis(3-carboxy-4-hydroxystyryl)benzene Iba1 ionized calcium binding adaptor molecule 1 JAK2 Janus activated kinase-2 MAPKK mitogen-activated protein kinase kinase MLA methyllycaconitine nAChR nicotinic acetylcholine receptor PI3K phosphoinositide-3 kinase Rac1 Ras-related C3 botulinum toxin substrate 1 TBS Tris-buffered saline MWM Morris water maze WAVE Wiskott-Aldrich syndrome protein family verprolin-homologous protein. ..

    Chick Chorioallantoic Membrane Assay:

    Article Title: Galantamine-induced Amyloid-? Clearance Mediated via Stimulation of Microglial Nicotinic Acetylcholine Receptors *
    Article Snippet: .. 4 The abbreviations used are: AD Alzheimer disease Aβ amyloid-β Aβ42 Aβ1–42 AChE acetylcholinesterase α7 nAChR α7 subunit of nAChR APL allosterically potentiating ligand Ca2+ (−) DMEM Ca2+ -free DMEM Ca2+ (+) DMEM Ca2+ -free DMEM supplemented with 1.8 m m Ca2+ CaM calmodulin CaMKII Ca2+ /CaM-dependent protein kinase II choline (−) choline-free choline (+) DMEM choline (−) DMEM supplemented with 28 μ m choline FA formic acid FSB 1-fluoro-2,5-bis(3-carboxy-4-hydroxystyryl)benzene Iba1 ionized calcium binding adaptor molecule 1 JAK2 Janus activated kinase-2 MAPKK mitogen-activated protein kinase kinase MLA methyllycaconitine nAChR nicotinic acetylcholine receptor PI3K phosphoinositide-3 kinase Rac1 Ras-related C3 botulinum toxin substrate 1 TBS Tris-buffered saline MWM Morris water maze WAVE Wiskott-Aldrich syndrome protein family verprolin-homologous protein. ..

    Plasmid Preparation:

    Article Title: VMAT2 and dopamine neuron loss in a primate model of Parkinson’s disease
    Article Snippet: .. Sections were incubated with antibodies against Glial fibrillary acidic protein (1 : 5000, rabbit, Dako, Carprinteria, CA, USA), LN3 antibody (1 : 500, mouse, Novocastra, Newcastle, UK), tyrosine hydroxylase (TH) (1 : 2000, rabbit, Chemicon, Temecula, CA, USA), nitrotyrosine (NT; 1 : 500, rabbit, Chemicon), and α-synuclein (1 : 5000, Chemicon; with or without pre-treatment with 20% formic acid) at 4°C for 48 h. After rinsing in TBS, sections were incubated with biotinylated secondary antibodies (1 : 250, Vector, Burlingame, CA, USA) for 60 min and the avidin–biotin peroxidase complex solution (1 : 1600, ABC Elite, Vector) for 60 min at 22–24°C. ..

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  • 90
    Millipore tle
    Tle, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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