scn8a exon 18  (Roche)


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    Structured Review

    Roche scn8a exon 18
    Alternative splicing of <t>SCN8A</t> <t>exon</t> 18 in the neoplasia-carcinoma sequence of human cervical tissue. ( A ) Alternative splicing of SCN8A Exon 18. Expanded genomic structure of exons 17 to 19. Exon 18 N contains an in frame stop codon. Splice variants generated by alternative splicing of Exon 18 are indicated with the PCR product length expected by using primers located in Exons 17 and 19 (see Methods). ( B – E ) End-point PCR electrophoresis results for SCN8A exon 18 variants expressed in non-cancerous cervix, cervical intraepithelial neoplasia, invasive cervical cancer, and cervical cancer cell lines, respectively. HEK-Nav1.6 cells was used as positive control for the adult splice form of SCN8A (18A; far-right line). A 100-bp molecular weight marker was used as reference (far-left line). The SCN8A variants generated for alternative splicing of exon 18: 18A, 18N and Δ18, were identified in the indicated group of samples. Identity of the SCN8A splice forms was confirmed by automated sequencing. The SCN8A splice forms were relatively more abundant in human cervical cancer samples, more clearly for the Δ18 variant; whereas the adult (18A) variant was practically absent in CeCa cell lines. From the two samples (266 and 275) that were present in all western blots experiments, only mRNA from sample 266 was available for performing these PCR analysis.
    Scn8a Exon 18, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 74 article reviews
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    scn8a exon 18 - by Bioz Stars, 2020-09
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    Images

    1) Product Images from "The invasiveness of human cervical cancer associated to the function of NaV1.6 channels is mediated by MMP-2 activity"

    Article Title: The invasiveness of human cervical cancer associated to the function of NaV1.6 channels is mediated by MMP-2 activity

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-31364-y

    Alternative splicing of SCN8A exon 18 in the neoplasia-carcinoma sequence of human cervical tissue. ( A ) Alternative splicing of SCN8A Exon 18. Expanded genomic structure of exons 17 to 19. Exon 18 N contains an in frame stop codon. Splice variants generated by alternative splicing of Exon 18 are indicated with the PCR product length expected by using primers located in Exons 17 and 19 (see Methods). ( B – E ) End-point PCR electrophoresis results for SCN8A exon 18 variants expressed in non-cancerous cervix, cervical intraepithelial neoplasia, invasive cervical cancer, and cervical cancer cell lines, respectively. HEK-Nav1.6 cells was used as positive control for the adult splice form of SCN8A (18A; far-right line). A 100-bp molecular weight marker was used as reference (far-left line). The SCN8A variants generated for alternative splicing of exon 18: 18A, 18N and Δ18, were identified in the indicated group of samples. Identity of the SCN8A splice forms was confirmed by automated sequencing. The SCN8A splice forms were relatively more abundant in human cervical cancer samples, more clearly for the Δ18 variant; whereas the adult (18A) variant was practically absent in CeCa cell lines. From the two samples (266 and 275) that were present in all western blots experiments, only mRNA from sample 266 was available for performing these PCR analysis.
    Figure Legend Snippet: Alternative splicing of SCN8A exon 18 in the neoplasia-carcinoma sequence of human cervical tissue. ( A ) Alternative splicing of SCN8A Exon 18. Expanded genomic structure of exons 17 to 19. Exon 18 N contains an in frame stop codon. Splice variants generated by alternative splicing of Exon 18 are indicated with the PCR product length expected by using primers located in Exons 17 and 19 (see Methods). ( B – E ) End-point PCR electrophoresis results for SCN8A exon 18 variants expressed in non-cancerous cervix, cervical intraepithelial neoplasia, invasive cervical cancer, and cervical cancer cell lines, respectively. HEK-Nav1.6 cells was used as positive control for the adult splice form of SCN8A (18A; far-right line). A 100-bp molecular weight marker was used as reference (far-left line). The SCN8A variants generated for alternative splicing of exon 18: 18A, 18N and Δ18, were identified in the indicated group of samples. Identity of the SCN8A splice forms was confirmed by automated sequencing. The SCN8A splice forms were relatively more abundant in human cervical cancer samples, more clearly for the Δ18 variant; whereas the adult (18A) variant was practically absent in CeCa cell lines. From the two samples (266 and 275) that were present in all western blots experiments, only mRNA from sample 266 was available for performing these PCR analysis.

    Techniques Used: Sequencing, Generated, Polymerase Chain Reaction, Electrophoresis, Positive Control, Molecular Weight, Marker, Variant Assay, Western Blot

    2) Product Images from "The invasiveness of human cervical cancer associated to the function of NaV1.6 channels is mediated by MMP-2 activity"

    Article Title: The invasiveness of human cervical cancer associated to the function of NaV1.6 channels is mediated by MMP-2 activity

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-31364-y

    Alternative splicing of SCN8A exon 18 in the neoplasia-carcinoma sequence of human cervical tissue. ( A ) Alternative splicing of SCN8A Exon 18. Expanded genomic structure of exons 17 to 19. Exon 18 N contains an in frame stop codon. Splice variants generated by alternative splicing of Exon 18 are indicated with the PCR product length expected by using primers located in Exons 17 and 19 (see Methods). ( B – E ) End-point PCR electrophoresis results for SCN8A exon 18 variants expressed in non-cancerous cervix, cervical intraepithelial neoplasia, invasive cervical cancer, and cervical cancer cell lines, respectively. HEK-Nav1.6 cells was used as positive control for the adult splice form of SCN8A (18A; far-right line). A 100-bp molecular weight marker was used as reference (far-left line). The SCN8A variants generated for alternative splicing of exon 18: 18A, 18N and Δ18, were identified in the indicated group of samples. Identity of the SCN8A splice forms was confirmed by automated sequencing. The SCN8A splice forms were relatively more abundant in human cervical cancer samples, more clearly for the Δ18 variant; whereas the adult (18A) variant was practically absent in CeCa cell lines. From the two samples (266 and 275) that were present in all western blots experiments, only mRNA from sample 266 was available for performing these PCR analysis.
    Figure Legend Snippet: Alternative splicing of SCN8A exon 18 in the neoplasia-carcinoma sequence of human cervical tissue. ( A ) Alternative splicing of SCN8A Exon 18. Expanded genomic structure of exons 17 to 19. Exon 18 N contains an in frame stop codon. Splice variants generated by alternative splicing of Exon 18 are indicated with the PCR product length expected by using primers located in Exons 17 and 19 (see Methods). ( B – E ) End-point PCR electrophoresis results for SCN8A exon 18 variants expressed in non-cancerous cervix, cervical intraepithelial neoplasia, invasive cervical cancer, and cervical cancer cell lines, respectively. HEK-Nav1.6 cells was used as positive control for the adult splice form of SCN8A (18A; far-right line). A 100-bp molecular weight marker was used as reference (far-left line). The SCN8A variants generated for alternative splicing of exon 18: 18A, 18N and Δ18, were identified in the indicated group of samples. Identity of the SCN8A splice forms was confirmed by automated sequencing. The SCN8A splice forms were relatively more abundant in human cervical cancer samples, more clearly for the Δ18 variant; whereas the adult (18A) variant was practically absent in CeCa cell lines. From the two samples (266 and 275) that were present in all western blots experiments, only mRNA from sample 266 was available for performing these PCR analysis.

    Techniques Used: Sequencing, Generated, Polymerase Chain Reaction, Electrophoresis, Positive Control, Molecular Weight, Marker, Variant Assay, Western Blot

    Related Articles

    Polymerase Chain Reaction:

    Article Title: The invasiveness of human cervical cancer associated to the function of NaV1.6 channels is mediated by MMP-2 activity
    Article Snippet: .. The first strand cDNA product (250 ng) was used as template in 10 µl PCR reactions with final concentrations of 200 µM dNTP, 0.3 µM primers for SCN8A Exon 18 (forward primer 5′-AAGTGGACAGCCTATGGCTTCG-3′, reverse primer 5′-TGTTGACATCTTCAATTTCAAATCGG-3′), 1.5 mM MgCl2 , and 1.3 U of enzyme mix (Expand High Fidelity PCR System, Roche Diagnostics; Mannheim, Germany). ..

    Article Title: The invasiveness of human cervical cancer associated to the function of NaV1.6 channels is mediated by MMP-2 activity
    Article Snippet: .. The first strand cDNA product (250 ng) was used as template in 10 µl PCR reactions with final concentrations of 200 µM dNTP, 0.3 µM primers for SCN8A Exon 18 (forward primer 5′-AAGTGGACAGCCTATGGCTTCG-3′, reverse primer 5′-TGTTGACATCTTCAATTTCAAATCGG-3′), 1.5 mM MgCl2 , and 1.3 U of enzyme mix (Expand High Fidelity PCR System, Roche Diagnostics; Mannheim, Germany). ..

    Article Title: Incorporation of a non-human glycan mediates human susceptibility to a bacterial toxin
    Article Snippet: .. For the S12A mutant, this involved high fidelity PCR amplification (Expand® High Fidelity PCR kit; Roche Molecular Diagnostics, Germany) of subAB -positive O113:H21 STEC DNA using primer pairs pETsubAF / SubBA12R and SubBA12F / pETsubBR ( ). .. This generated two fragments with the necessary mutation in the S12 codon of subB incorporated into the overlapping region by the SubBA12R and SubBA12F primers.

    Mutagenesis:

    Article Title: Incorporation of a non-human glycan mediates human susceptibility to a bacterial toxin
    Article Snippet: .. For the S12A mutant, this involved high fidelity PCR amplification (Expand® High Fidelity PCR kit; Roche Molecular Diagnostics, Germany) of subAB -positive O113:H21 STEC DNA using primer pairs pETsubAF / SubBA12R and SubBA12F / pETsubBR ( ). .. This generated two fragments with the necessary mutation in the S12 codon of subB incorporated into the overlapping region by the SubBA12R and SubBA12F primers.

    Amplification:

    Article Title: Incorporation of a non-human glycan mediates human susceptibility to a bacterial toxin
    Article Snippet: .. For the S12A mutant, this involved high fidelity PCR amplification (Expand® High Fidelity PCR kit; Roche Molecular Diagnostics, Germany) of subAB -positive O113:H21 STEC DNA using primer pairs pETsubAF / SubBA12R and SubBA12F / pETsubBR ( ). .. This generated two fragments with the necessary mutation in the S12 codon of subB incorporated into the overlapping region by the SubBA12R and SubBA12F primers.

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    Roche fidelity pcr amplification expand high fidelity pcr kit roche molecular diagnostics germany
    Fidelity Pcr Amplification Expand High Fidelity Pcr Kit Roche Molecular Diagnostics Germany, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fidelity pcr amplification expand high fidelity pcr kit roche molecular diagnostics germany/product/Roche
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    fidelity pcr amplification expand high fidelity pcr kit roche molecular diagnostics germany - by Bioz Stars, 2020-09
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