ficoll paque  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ficoll paque
    Ficoll Paque, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque/product/Thermo Fisher
    Average 92 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    ficoll paque - by Bioz Stars, 2020-07
    92/100 stars

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    Centrifugation:

    Article Title: Comparison of the therapeutic effectiveness of human CD34+ and rat bone marrow mesenchymal stem cells on improvement of experimental liver fibrosis in Wistar rats
    Article Snippet: .. Briefly, the MNCs were separated by centrifugation over a Ficoll-Paque (Gibco-Invitrogen, Grand Island, NY) density gradient at 400 × g for 35 minutes at 10°C. .. The MNC fraction was washed first in PBS, then with MACS (magnetic cell sorting) buffer (PBS supplemented with 0.5% bovine serum albumin and 2 mM EDTA, pH 7.2).

    Article Title: Circulating Endothelial Cells and Endothelial Function predict Major Adverse Cardiac Events and Early Adverse Left Ventricular Remodeling in Patients with ST-Segment Elevation Myocardial Infarction
    Article Snippet: .. Peripheral blood mononuclear cells (PB-MNCs) fraction were isolated using density-gradient centrifugation with Ficoll-Paque (Gibco-Invitrogen, Grand Island, NY). .. PB-MNCs were cultured in endothelial culture medium (EC basal medium-2; Lonza-Walkersville, MD, USA) and incubated at 37°C with 5% CO2 for 14 days.

    Gradient Centrifugation:

    Article Title: Skeletal stem cell‐mediated suppression on inflammatory osteoclastogenesis occurs via concerted action of cell adhesion molecules and osteoprotegerin, et al. Skeletal stem cell‐mediated suppression on inflammatory osteoclastogenesis occurs via concerted action of cell adhesion molecules and osteoprotegerin
    Article Snippet: .. The murine bone marrow mononuclear cells were harvested from mouse (6‐8 weeks old) femur and tibia bone marrow suspensions with Ficoll‐Paque (1.073 g/mL; Invitrogen) density gradient centrifugation. .. Furthermore, bone marrow mononuclear cells were suspended at a concentration of 107 cells/mL in PBS that contained 2% FBS (Hyclone) and stained with 10 μM carboxyfluorescein diacetate succinimidyl ester (CFSE).

    Article Title: Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer
    Article Snippet: .. PMNs were isolated using Ficoll-Paque followed by dextran (1%) density gradient centrifugation as described previously and fluorescein-labeled with BCECF-AM (Molecular Probes, Eugene, OR) for 30 minutes at 37 °C. .. The percentage of PMNs in the tumor digestion mixture was determined by FACS analysis after washing mixture through a 70 μm nylon strainer and labeling with FITC-conjugated anti-Ly6G antibody (BioLegend, San Diego, CA).

    Article Title: IgA Fc-folate conjugate activates and recruits neutrophils to directly target triple negative breast cancer cells
    Article Snippet: .. PMNs were isolated using Ficoll-Paque followed by dextran (1%) density gradient centrifugation as described previously [ ] and labeled with 10 μg/ml BCECF-AM (ThermoFisher Scientific) for 30 minutes at 37°C just before use. .. GIPZ Lentiviral particles containing FRA shRNA #4 vector ( shFRA ) or non-silenced control vector (NS) were obtained from Karmanos Cancer Institute by subscription to Thermo Scientific GIPZ shRNAmir library.

    Article Title: Recombinant chicken interleukin-7 as a potent adjuvant increases the immunogenicity and protection of inactivated infectious bursal disease vaccine
    Article Snippet: .. Splenic lymphocytes were separated by density gradient centrifugation on Ficoll-Paque and washed twice in fresh RPMI 1640 medium (Invitrogen). .. The cells were resuspended at 2 × 106 cells/mL in RPMI 1640 medium with 10% fetal bovine serum (FBS), 100 units/mL penicillin, 100 μg/mL streptomycin, and 2 mM l -glutamine, 100 mL cells were added to 96-well plates and stimulated in vitro for 72 h at 37 °C in a 5% CO2 incubator with either concanavalin A (Con A, 5 μg/mL, Sigma) as a positive control, or recombinant VP2 protein (5 μg/mL) as specific antigen.

    Isolation:

    Article Title: Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer
    Article Snippet: .. PMNs were isolated using Ficoll-Paque followed by dextran (1%) density gradient centrifugation as described previously and fluorescein-labeled with BCECF-AM (Molecular Probes, Eugene, OR) for 30 minutes at 37 °C. .. The percentage of PMNs in the tumor digestion mixture was determined by FACS analysis after washing mixture through a 70 μm nylon strainer and labeling with FITC-conjugated anti-Ly6G antibody (BioLegend, San Diego, CA).

    Article Title: Circulating Endothelial Cells and Endothelial Function predict Major Adverse Cardiac Events and Early Adverse Left Ventricular Remodeling in Patients with ST-Segment Elevation Myocardial Infarction
    Article Snippet: .. Peripheral blood mononuclear cells (PB-MNCs) fraction were isolated using density-gradient centrifugation with Ficoll-Paque (Gibco-Invitrogen, Grand Island, NY). .. PB-MNCs were cultured in endothelial culture medium (EC basal medium-2; Lonza-Walkersville, MD, USA) and incubated at 37°C with 5% CO2 for 14 days.

    Article Title: Suppression of arthritic bone destruction by adenovirus-mediated csk gene transfer to synoviocytes and osteoclasts
    Article Snippet: .. PBMCs isolated using Ficoll-Paque were cocultured with rheumatoid synovial fibroblasts in α-MEM containing 20% horse serum (GIBCO BRL) in the presence of 10–7 M 1,25(OH)2 vitamin D3 (Wako Pure Chemicals Industries) and 300 U/mL recombinant human M-CSF (Austral Biologicals, San Ramon, California, USA). .. After 3 weeks, multinucleated OCLs formed in coculture were isolated by pronase E treatment.

    Article Title: IgA Fc-folate conjugate activates and recruits neutrophils to directly target triple negative breast cancer cells
    Article Snippet: .. PMNs were isolated using Ficoll-Paque followed by dextran (1%) density gradient centrifugation as described previously [ ] and labeled with 10 μg/ml BCECF-AM (ThermoFisher Scientific) for 30 minutes at 37°C just before use. .. GIPZ Lentiviral particles containing FRA shRNA #4 vector ( shFRA ) or non-silenced control vector (NS) were obtained from Karmanos Cancer Institute by subscription to Thermo Scientific GIPZ shRNAmir library.

    Labeling:

    Article Title: IgA Fc-folate conjugate activates and recruits neutrophils to directly target triple negative breast cancer cells
    Article Snippet: .. PMNs were isolated using Ficoll-Paque followed by dextran (1%) density gradient centrifugation as described previously [ ] and labeled with 10 μg/ml BCECF-AM (ThermoFisher Scientific) for 30 minutes at 37°C just before use. .. GIPZ Lentiviral particles containing FRA shRNA #4 vector ( shFRA ) or non-silenced control vector (NS) were obtained from Karmanos Cancer Institute by subscription to Thermo Scientific GIPZ shRNAmir library.

    other:

    Article Title: Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury?
    Article Snippet: 35 mL of the diluted sample was carefully layered using 15 mL Ficoll-Paque (Gibco-Invitrogen, Grand Island, NY, USA).

    Recombinant:

    Article Title: Suppression of arthritic bone destruction by adenovirus-mediated csk gene transfer to synoviocytes and osteoclasts
    Article Snippet: .. PBMCs isolated using Ficoll-Paque were cocultured with rheumatoid synovial fibroblasts in α-MEM containing 20% horse serum (GIBCO BRL) in the presence of 10–7 M 1,25(OH)2 vitamin D3 (Wako Pure Chemicals Industries) and 300 U/mL recombinant human M-CSF (Austral Biologicals, San Ramon, California, USA). .. After 3 weeks, multinucleated OCLs formed in coculture were isolated by pronase E treatment.

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    Thermo Fisher ficoll paque plus pharmacia
    Ficoll Paque Plus Pharmacia, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ficoll paque density gradient centrifugation method
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    https://www.bioz.com/result/ficoll paque density gradient centrifugation method/product/Thermo Fisher
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    Thermo Fisher ficoll paque
    Ficoll Paque, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque/product/Thermo Fisher
    Average 92 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    ficoll paque - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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