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STEMCELL Technologies Inc ficoll paque
Ficoll Paque, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 93/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ficoll paque/product/STEMCELL Technologies Inc
Average 93 stars, based on 65 article reviews
Price from $9.99 to $1999.99
ficoll paque - by Bioz Stars, 2020-07
93/100 stars

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Centrifugation:

Article Title: Cholecalciferol Supplementation Alters Calcitriol-Responsive Monocyte Proteins and Decreases Inflammatory Cytokines in ESRD
Article Snippet: .. For monocyte isolation, 30 ml of whole blood was collected in phlebotomy tubes containing EDTA anticoagulant and subsequently processed using Ficoll-Paque (Stem Cell Technologies, Vancouver, British Columbia, Canada) centrifugation to obtain the lymphocyte fraction. .. Ficoll-Paque centrifugation was performed by first mixing 15 ml of whole blood with 15 ml of 1× PBS supplemented with 2% FBS, then layering this mixture over 15 ml of Ficoll-Paque solution in a 50-ml conical bottom tube.

Article Title: Soluble Toll-Like Receptor 4 Impairs the Interaction of Shiga Toxin 2a with Human Serum Amyloid P Component
Article Snippet: .. Highly pure neutrophils (99.7%) were isolated under endotoxin-low conditions from buffy coats of healthy donors after centrifugation over Ficoll-Paque, followed by dextran sedimentation, hypotonic lysis of contaminating erythrocytes, and removing of any eventual contaminating cells by using the EasySep human neutrophil enrichment kit (Stemcell Technologies, Vancouver, BC, Canada) [ , ]. .. For binding experiments with purified Stx1a and Stx2a, Eppendorf tubes were precoated with PBS containing 1% endotoxin-low (≤1 endotoxin unit/mg) BSA to avoid nonspecific loss of the toxins.

Gradient Centrifugation:

Article Title: Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice
Article Snippet: .. PBMCs were isolated from leukopaks by gradient centrifugation on Ficoll-Paque for 30 minutes at 600 g. PBMCs collected from the buffy coat were stimulated with PHA (5 µg/ml) for 24 h in RPMI with 10% FBS and gentamicin (10 µg/ml) supplemented with human recombinant interleukin-2 (rIL-2) at a concentration of 30 ng/ml ((STEMCELL Technologies, Seattle, WA). .. Cell culture HIV-1 infection HEK-293T cells were transfected using CaPO4 precipitation method in the presence of chloroquine (50 µM) with 30 µg of pNL4–3 -EGFP-P2A-Nef plasmid /2.5 × 106 cells/100 mm dish.

Sedimentation:

Article Title: Soluble Toll-Like Receptor 4 Impairs the Interaction of Shiga Toxin 2a with Human Serum Amyloid P Component
Article Snippet: .. Highly pure neutrophils (99.7%) were isolated under endotoxin-low conditions from buffy coats of healthy donors after centrifugation over Ficoll-Paque, followed by dextran sedimentation, hypotonic lysis of contaminating erythrocytes, and removing of any eventual contaminating cells by using the EasySep human neutrophil enrichment kit (Stemcell Technologies, Vancouver, BC, Canada) [ , ]. .. For binding experiments with purified Stx1a and Stx2a, Eppendorf tubes were precoated with PBS containing 1% endotoxin-low (≤1 endotoxin unit/mg) BSA to avoid nonspecific loss of the toxins.

Isolation:

Article Title: Chediak-Higashi syndrome: LYST domains regulate exocytosis of lytic granules, but not cytokine secretion by NK cells
Article Snippet: .. PBMCs were isolated from whole blood using Ficoll-Paque method, NK cells were isolated using EasySep Human NK cell kits (StemCell Technologies), and cultured in X-vivo medium supplemented with IL-2 (100U/ml). .. IL-2 cultured NK cells were used in experiments, unless otherwise noted.

Article Title: Cholecalciferol Supplementation Alters Calcitriol-Responsive Monocyte Proteins and Decreases Inflammatory Cytokines in ESRD
Article Snippet: .. For monocyte isolation, 30 ml of whole blood was collected in phlebotomy tubes containing EDTA anticoagulant and subsequently processed using Ficoll-Paque (Stem Cell Technologies, Vancouver, British Columbia, Canada) centrifugation to obtain the lymphocyte fraction. .. Ficoll-Paque centrifugation was performed by first mixing 15 ml of whole blood with 15 ml of 1× PBS supplemented with 2% FBS, then layering this mixture over 15 ml of Ficoll-Paque solution in a 50-ml conical bottom tube.

Article Title: Soluble Toll-Like Receptor 4 Impairs the Interaction of Shiga Toxin 2a with Human Serum Amyloid P Component
Article Snippet: .. Highly pure neutrophils (99.7%) were isolated under endotoxin-low conditions from buffy coats of healthy donors after centrifugation over Ficoll-Paque, followed by dextran sedimentation, hypotonic lysis of contaminating erythrocytes, and removing of any eventual contaminating cells by using the EasySep human neutrophil enrichment kit (Stemcell Technologies, Vancouver, BC, Canada) [ , ]. .. For binding experiments with purified Stx1a and Stx2a, Eppendorf tubes were precoated with PBS containing 1% endotoxin-low (≤1 endotoxin unit/mg) BSA to avoid nonspecific loss of the toxins.

Article Title: Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice
Article Snippet: .. PBMCs were isolated from leukopaks by gradient centrifugation on Ficoll-Paque for 30 minutes at 600 g. PBMCs collected from the buffy coat were stimulated with PHA (5 µg/ml) for 24 h in RPMI with 10% FBS and gentamicin (10 µg/ml) supplemented with human recombinant interleukin-2 (rIL-2) at a concentration of 30 ng/ml ((STEMCELL Technologies, Seattle, WA). .. Cell culture HIV-1 infection HEK-293T cells were transfected using CaPO4 precipitation method in the presence of chloroquine (50 µM) with 30 µg of pNL4–3 -EGFP-P2A-Nef plasmid /2.5 × 106 cells/100 mm dish.

Cell Culture:

Article Title: Chediak-Higashi syndrome: LYST domains regulate exocytosis of lytic granules, but not cytokine secretion by NK cells
Article Snippet: .. PBMCs were isolated from whole blood using Ficoll-Paque method, NK cells were isolated using EasySep Human NK cell kits (StemCell Technologies), and cultured in X-vivo medium supplemented with IL-2 (100U/ml). .. IL-2 cultured NK cells were used in experiments, unless otherwise noted.

Concentration Assay:

Article Title: Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice
Article Snippet: .. PBMCs were isolated from leukopaks by gradient centrifugation on Ficoll-Paque for 30 minutes at 600 g. PBMCs collected from the buffy coat were stimulated with PHA (5 µg/ml) for 24 h in RPMI with 10% FBS and gentamicin (10 µg/ml) supplemented with human recombinant interleukin-2 (rIL-2) at a concentration of 30 ng/ml ((STEMCELL Technologies, Seattle, WA). .. Cell culture HIV-1 infection HEK-293T cells were transfected using CaPO4 precipitation method in the presence of chloroquine (50 µM) with 30 µg of pNL4–3 -EGFP-P2A-Nef plasmid /2.5 × 106 cells/100 mm dish.

other:

Article Title: Prenatal Maternal Physical Activity and Stem Cells in Umbilical Cord Blood
Article Snippet: Samples were processed for mononuclear cells (MNC) using a Ficoll-Paque (STEMCELL Technologies, Vancouver, Canada) density gradient within 24 hour of birth as described previously ( ).

Lysis:

Article Title: Soluble Toll-Like Receptor 4 Impairs the Interaction of Shiga Toxin 2a with Human Serum Amyloid P Component
Article Snippet: .. Highly pure neutrophils (99.7%) were isolated under endotoxin-low conditions from buffy coats of healthy donors after centrifugation over Ficoll-Paque, followed by dextran sedimentation, hypotonic lysis of contaminating erythrocytes, and removing of any eventual contaminating cells by using the EasySep human neutrophil enrichment kit (Stemcell Technologies, Vancouver, BC, Canada) [ , ]. .. For binding experiments with purified Stx1a and Stx2a, Eppendorf tubes were precoated with PBS containing 1% endotoxin-low (≤1 endotoxin unit/mg) BSA to avoid nonspecific loss of the toxins.

Recombinant:

Article Title: Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice
Article Snippet: .. PBMCs were isolated from leukopaks by gradient centrifugation on Ficoll-Paque for 30 minutes at 600 g. PBMCs collected from the buffy coat were stimulated with PHA (5 µg/ml) for 24 h in RPMI with 10% FBS and gentamicin (10 µg/ml) supplemented with human recombinant interleukin-2 (rIL-2) at a concentration of 30 ng/ml ((STEMCELL Technologies, Seattle, WA). .. Cell culture HIV-1 infection HEK-293T cells were transfected using CaPO4 precipitation method in the presence of chloroquine (50 µM) with 30 µg of pNL4–3 -EGFP-P2A-Nef plasmid /2.5 × 106 cells/100 mm dish.

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    STEMCELL Technologies Inc ficoll paque plus
    Ficoll Paque Plus, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque plus/product/STEMCELL Technologies Inc
    Average 93 stars, based on 9 article reviews
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    ficoll paque plus - by Bioz Stars, 2020-07
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    91
    STEMCELL Technologies Inc ficoll paque premium density gradient media
    Ficoll Paque Premium Density Gradient Media, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque premium density gradient media/product/STEMCELL Technologies Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ficoll paque premium density gradient media - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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