Structured Review

PAA Laboratories ficoll paque
Ficoll Paque, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ficoll paque/product/PAA Laboratories
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
ficoll paque - by Bioz Stars, 2020-07
92/100 stars

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Isolation:

Article Title: Adjuvant effects of aluminium hydroxide-adsorbed allergens and allergoids – differences in vivo and in vitro
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll Paque 1077 g/ml (PAA Laboratories GmbH, Cölbe, Germany) density centrifugation from heparinized blood. .. CD14+ cells were enriched by incubation of 5 × 106 PBMC in a 12-well-plate (Greiner, Frickenhausen, Germany) with 1 ml/well Iscove's modified Dulbecco's medium containing L-glutamine and 25 mM Hepes (IMDM; PAA Laboratories GmbH) and 3% autologous heat-inactivated plasma at 37°C for 45 min. Non-adherent cells were washed twice with prewarmed PBS.

Article Title: Helicobacter pylori Stimulates Dendritic Cells To Induce Interleukin-17 Expression from CD4+ T Lymphocytes ▿
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats (National Blood Transfusion Centre, South Thames, United Kingdom) through Ficoll-Paque (PAA Laboratories Ltd., United Kingdom) gradient centrifugation. .. Monocyte-derived DCs were generated from CD14+ cells isolated by using CD14 beads (Miltenyi Biotec, Surrey, United Kingdom) as previously described ( ).

Article Title: Quantifying Cathepsin S Activity in Antigen Presenting Cells Using a Novel Specific Substrate *
Article Snippet: .. Peripheral blood mononuclear cells were isolated by Ficoll/Paque (PAA Laboratories, Pasching, Austria) density gradient centrifugation of heparinized blood obtained from buffy coats. .. Isolated peripheral blood mononuclear cells were plated (DCs: 1 × 108 cells/8 ml/flask; macrophages: 8 × 107 cells/10 ml/flask) into 75-cm2 tissue culture flasks (DCs: Cell Star, Greiner Bio-One GmbH, Frickenhausen, Germany; macrophages: BD Primaria T 75, BD Biosciences, San Jose, CA) in RPMI 1640 (Invitrogen) under the same culture conditions as for WT100.

Centrifugation:

Article Title: Adjuvant effects of aluminium hydroxide-adsorbed allergens and allergoids – differences in vivo and in vitro
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll Paque 1077 g/ml (PAA Laboratories GmbH, Cölbe, Germany) density centrifugation from heparinized blood. .. CD14+ cells were enriched by incubation of 5 × 106 PBMC in a 12-well-plate (Greiner, Frickenhausen, Germany) with 1 ml/well Iscove's modified Dulbecco's medium containing L-glutamine and 25 mM Hepes (IMDM; PAA Laboratories GmbH) and 3% autologous heat-inactivated plasma at 37°C for 45 min. Non-adherent cells were washed twice with prewarmed PBS.

Gradient Centrifugation:

Article Title: Helicobacter pylori Stimulates Dendritic Cells To Induce Interleukin-17 Expression from CD4+ T Lymphocytes ▿
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats (National Blood Transfusion Centre, South Thames, United Kingdom) through Ficoll-Paque (PAA Laboratories Ltd., United Kingdom) gradient centrifugation. .. Monocyte-derived DCs were generated from CD14+ cells isolated by using CD14 beads (Miltenyi Biotec, Surrey, United Kingdom) as previously described ( ).

Article Title: Inhibition of cathepsin X enzyme influences the immune response of THP-1 cells and dendritic cells infected with Helicobacter pylori
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were obtained through Ficoll-Paque (PAA Laboratories Ltd., UK) gradient centrifugation. .. Monocyte-derived DCs were generated from CD14+ cells isolated by using CD14 coated immunomagnetic beads (DynabeadsFlowComp, Dynal, Norway).

Article Title: Quantifying Cathepsin S Activity in Antigen Presenting Cells Using a Novel Specific Substrate *
Article Snippet: .. Peripheral blood mononuclear cells were isolated by Ficoll/Paque (PAA Laboratories, Pasching, Austria) density gradient centrifugation of heparinized blood obtained from buffy coats. .. Isolated peripheral blood mononuclear cells were plated (DCs: 1 × 108 cells/8 ml/flask; macrophages: 8 × 107 cells/10 ml/flask) into 75-cm2 tissue culture flasks (DCs: Cell Star, Greiner Bio-One GmbH, Frickenhausen, Germany; macrophages: BD Primaria T 75, BD Biosciences, San Jose, CA) in RPMI 1640 (Invitrogen) under the same culture conditions as for WT100.

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    PAA Laboratories ficoll paque
    Ficoll Paque, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque/product/PAA Laboratories
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ficoll paque - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    PAA Laboratories ficoll paque 1
    Ficoll Paque 1, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque 1/product/PAA Laboratories
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ficoll paque 1 - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

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