Structured Review

Nycomed ficoll paque
Ficoll Paque, supplied by Nycomed, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ficoll paque/product/Nycomed
Average 92 stars, based on 14 article reviews
Price from $9.99 to $1999.99
ficoll paque - by Bioz Stars, 2020-07
92/100 stars

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Centrifugation:

Article Title: T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy?
Article Snippet: .. The resultant suspension was divided into 10 ml aliquots and separated by density centrifugation over Ficoll-Paque (Nycomed, UK) at 400 g for 25 min. ..

Article Title: Histamine Promotes the Development of Monocyte-Derived Dendritic Cells and Reduces Tumor Growth by Targeting the Myeloid NADPH Oxidase
Article Snippet: .. PBMCs were prepared from blood donor buffy coats by Ficoll-Paque (Lymphoprep; Nycomed, Oslo, Norway) density centrifugation and were separated into CD14+ monocytes using iMag positive selection beads (BD Biosciences, San Diego, CA), according to the manufacturer’s instructions. .. The isolation procedures resulted in > 98% pure monocytes.

Selection:

Article Title: Histamine Promotes the Development of Monocyte-Derived Dendritic Cells and Reduces Tumor Growth by Targeting the Myeloid NADPH Oxidase
Article Snippet: .. PBMCs were prepared from blood donor buffy coats by Ficoll-Paque (Lymphoprep; Nycomed, Oslo, Norway) density centrifugation and were separated into CD14+ monocytes using iMag positive selection beads (BD Biosciences, San Diego, CA), according to the manufacturer’s instructions. .. The isolation procedures resulted in > 98% pure monocytes.

Filtration:

Article Title: Validation of a mouse xenograft model system for gene expression analysis of human acute lymphoblastic leukaemia
Article Snippet: .. Mononuclear cells from spleen samples were purified by syringe homogenisation, followed by filtration and density gradient centrifugation by Ficoll-Paque (n = 4) (LymphoPrep, Nycomed). .. Bone marrow cells were collected by flushing both femurs with RPMI 1640 medium (Life Technologies) into a collection tube without density gradient centrifugation (n = 4).

Homogenization:

Article Title: Validation of a mouse xenograft model system for gene expression analysis of human acute lymphoblastic leukaemia
Article Snippet: .. Mononuclear cells from spleen samples were purified by syringe homogenisation, followed by filtration and density gradient centrifugation by Ficoll-Paque (n = 4) (LymphoPrep, Nycomed). .. Bone marrow cells were collected by flushing both femurs with RPMI 1640 medium (Life Technologies) into a collection tube without density gradient centrifugation (n = 4).

Isolation:

Article Title: IVIg Immune Reconstitution Treatment Alleviates the State of Persistent Immune Activation and Suppressed CD4 T Cell Counts in CVID
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were isolated by density-gradient sedimentation using Ficoll-Paque (Lymphoprep, Nycomed Pharma, Oslo, Norway). .. Isolated PBMC were washed twice in Hank's balanced salt solution (Gibco, Grand Island, NY), and cryopreserved in RPMI 1640 (Gibco), supplemented with 20% heat inactivated fetal bovine serum (FBS; Hyclone Laboratories, Logan UT), 50 U/ml of penicillin (Gibco), 50 µg/ml of streptomycin (Gibco), 10 mM glutamine (Gibco) and 7.5% dimethylsulphoxide (DMSO; Sigma, St Louis, MO).

Article Title: MAIT cells are activated in acute Dengue virus infection and after in vitro Zika virus infection
Article Snippet: .. Sample collection Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient sedimentation using Ficoll-Paque (Lymphoprep, Nycomed Pharma, Oslo, Norway). .. Isolated PBMCs were washed twice in Hank’s balanced salt solution (Gibco, Grand Island, NY), and cryopreserved in RPMI 1640 (Gibco), supplemented with 20% heat inactivated fetal bovine serum (FBS; Hyclone Laboratories, Logan UT), 50 U/ml of penicillin (Gibco), 50 μg/ml of streptomycin (Gibco), 10 mM glutamine (Gibco) and 7.5% dimethylsulphoxide (DMSO; Sigma, St Louis, MO).

Article Title: Latent Mycobacterium tuberculosis Infection Is Associated With a Higher Frequency of Mucosal-Associated Invariant T and Invariant Natural Killer T Cells
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient sedimentation using Ficoll-Paque (Lymphoprep, Nycomed Pharma, Oslo, Norway). .. Isolated PBMCs were washed twice in Hank’s balanced salt solution (Gibco, Grand Island, NY, USA), and cryopreserved in RPMI 1640 (Gibco), supplemented with 20% heat inactivated fetal bovine serum (FBS; Hyclone Laboratories, Logan, UT, USA), 50 U/ml of penicillin (Gibco), 50 µg/ml of streptomycin (Gibco), 10 mM glutamine (Gibco), and 7.5% dimethylsulfoxide (Sigma, St. Louis, MO, USA).

Article Title: Prior Dengue Virus Exposure Shapes T Cell Immunity to Zika Virus in Humans
Article Snippet: .. PBMC were isolated by density-gradient sedimentation using Ficoll-Paque (Lymphoprep; Nycomed Pharma, Oslo, Norway) as previously described ( ). .. Isolated PBMC were cryopreserved in cell recovery media containing 10% dimethyl sulfoxide (DMSO) (Gibco), supplemented with 10 to 50% heat-inactivated fetal bovine serum (FBS; HyClone Laboratories, Logan UT), depending on the processing laboratory, and stored in liquid nitrogen until use in the assays.

Article Title: Latent Mycobacterium tuberculosis Infection Is Associated With a Higher Frequency of Mucosal-Associated Invariant T and Invariant Natural Killer T Cells
Article Snippet: .. Sample Collection Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient sedimentation using Ficoll-Paque (Lymphoprep, Nycomed Pharma, Oslo, Norway). .. Isolated PBMCs were washed twice in Hank’s balanced salt solution (Gibco, Grand Island, NY, USA), and cryopreserved in RPMI 1640 (Gibco), supplemented with 20% heat inactivated fetal bovine serum (FBS; Hyclone Laboratories, Logan, UT, USA), 50 U/ml of penicillin (Gibco), 50 µg/ml of streptomycin (Gibco), 10 mM glutamine (Gibco), and 7.5% dimethylsulfoxide (Sigma, St. Louis, MO, USA).

Purification:

Article Title: Validation of a mouse xenograft model system for gene expression analysis of human acute lymphoblastic leukaemia
Article Snippet: .. Mononuclear cells from spleen samples were purified by syringe homogenisation, followed by filtration and density gradient centrifugation by Ficoll-Paque (n = 4) (LymphoPrep, Nycomed). .. Bone marrow cells were collected by flushing both femurs with RPMI 1640 medium (Life Technologies) into a collection tube without density gradient centrifugation (n = 4).

Sedimentation:

Article Title: IVIg Immune Reconstitution Treatment Alleviates the State of Persistent Immune Activation and Suppressed CD4 T Cell Counts in CVID
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were isolated by density-gradient sedimentation using Ficoll-Paque (Lymphoprep, Nycomed Pharma, Oslo, Norway). .. Isolated PBMC were washed twice in Hank's balanced salt solution (Gibco, Grand Island, NY), and cryopreserved in RPMI 1640 (Gibco), supplemented with 20% heat inactivated fetal bovine serum (FBS; Hyclone Laboratories, Logan UT), 50 U/ml of penicillin (Gibco), 50 µg/ml of streptomycin (Gibco), 10 mM glutamine (Gibco) and 7.5% dimethylsulphoxide (DMSO; Sigma, St Louis, MO).

Article Title: MAIT cells are activated in acute Dengue virus infection and after in vitro Zika virus infection
Article Snippet: .. Sample collection Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient sedimentation using Ficoll-Paque (Lymphoprep, Nycomed Pharma, Oslo, Norway). .. Isolated PBMCs were washed twice in Hank’s balanced salt solution (Gibco, Grand Island, NY), and cryopreserved in RPMI 1640 (Gibco), supplemented with 20% heat inactivated fetal bovine serum (FBS; Hyclone Laboratories, Logan UT), 50 U/ml of penicillin (Gibco), 50 μg/ml of streptomycin (Gibco), 10 mM glutamine (Gibco) and 7.5% dimethylsulphoxide (DMSO; Sigma, St Louis, MO).

Article Title: Latent Mycobacterium tuberculosis Infection Is Associated With a Higher Frequency of Mucosal-Associated Invariant T and Invariant Natural Killer T Cells
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient sedimentation using Ficoll-Paque (Lymphoprep, Nycomed Pharma, Oslo, Norway). .. Isolated PBMCs were washed twice in Hank’s balanced salt solution (Gibco, Grand Island, NY, USA), and cryopreserved in RPMI 1640 (Gibco), supplemented with 20% heat inactivated fetal bovine serum (FBS; Hyclone Laboratories, Logan, UT, USA), 50 U/ml of penicillin (Gibco), 50 µg/ml of streptomycin (Gibco), 10 mM glutamine (Gibco), and 7.5% dimethylsulfoxide (Sigma, St. Louis, MO, USA).

Article Title: Prior Dengue Virus Exposure Shapes T Cell Immunity to Zika Virus in Humans
Article Snippet: .. PBMC were isolated by density-gradient sedimentation using Ficoll-Paque (Lymphoprep; Nycomed Pharma, Oslo, Norway) as previously described ( ). .. Isolated PBMC were cryopreserved in cell recovery media containing 10% dimethyl sulfoxide (DMSO) (Gibco), supplemented with 10 to 50% heat-inactivated fetal bovine serum (FBS; HyClone Laboratories, Logan UT), depending on the processing laboratory, and stored in liquid nitrogen until use in the assays.

Article Title: Latent Mycobacterium tuberculosis Infection Is Associated With a Higher Frequency of Mucosal-Associated Invariant T and Invariant Natural Killer T Cells
Article Snippet: .. Sample Collection Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient sedimentation using Ficoll-Paque (Lymphoprep, Nycomed Pharma, Oslo, Norway). .. Isolated PBMCs were washed twice in Hank’s balanced salt solution (Gibco, Grand Island, NY, USA), and cryopreserved in RPMI 1640 (Gibco), supplemented with 20% heat inactivated fetal bovine serum (FBS; Hyclone Laboratories, Logan, UT, USA), 50 U/ml of penicillin (Gibco), 50 µg/ml of streptomycin (Gibco), 10 mM glutamine (Gibco), and 7.5% dimethylsulfoxide (Sigma, St. Louis, MO, USA).

Gradient Centrifugation:

Article Title: Validation of a mouse xenograft model system for gene expression analysis of human acute lymphoblastic leukaemia
Article Snippet: .. Mononuclear cells from spleen samples were purified by syringe homogenisation, followed by filtration and density gradient centrifugation by Ficoll-Paque (n = 4) (LymphoPrep, Nycomed). .. Bone marrow cells were collected by flushing both femurs with RPMI 1640 medium (Life Technologies) into a collection tube without density gradient centrifugation (n = 4).

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  • 92
    Nycomed ficoll paque
    Ficoll Paque, supplied by Nycomed, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque/product/Nycomed
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    ficoll paque - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    Nycomed ficoll paque gradient centrifugation
    Ficoll Paque Gradient Centrifugation, supplied by Nycomed, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque gradient centrifugation/product/Nycomed
    Average 91 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    ficoll paque gradient centrifugation - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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