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Miltenyi Biotec ficoll paque
Ficoll Paque, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ficoll paque/product/Miltenyi Biotec
Average 93 stars, based on 29 article reviews
Price from $9.99 to $1999.99
ficoll paque - by Bioz Stars, 2020-07
93/100 stars

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Related Articles

Cell Isolation:

Article Title: Extended Freeze-Dried BCG Instructed pDCs Induce Suppressive Tregs and Dampen EAE
Article Snippet: .. PBMC from Buffy Coats were first isolated with Ficoll Paque and pDC purified with Diamond Plasmacytoid Dendritic Cell Isolation Kit II human according to the manufacturer (Miltenyi Biotec). .. For in vitro co-culture assay, purified pDCs (60 × 104 /200μl) were stimulated with 20μg/ml EFD BCG in complete RPMI medium for 16 h in presence of human IL-3 (10 ng/ml, PeproTech), extensively washed, and co-cultured with purified allogenic naïve CD4+ T cells (Naive CD4+ T Cell Isolation Kit II human, Miltenyi Biotec) at a 1/3 ratio (16'000 DC/50'000 T) for 6 days in triplicates.

Selection:

Article Title: Dipalmitoylphosphatidylcholine modulates inflammatory functions of monocytic cells independently of mitogen activated protein kinases
Article Snippet: .. Isolation of human monocytes from peripheral blood was performed by density centrifugation over Ficoll Paque® and negative selection of monocytes using midiMACS (Miltenyi Biotec, Camberley, U.K.). ..

Article Title: Oxidative Stress and Dietary Micronutrient Deficiencies Contribute to Overexpression of Epigenetically Regulated Genes by Lupus T Cells
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by density gradient centrifugation on Ficoll-Paque, cultured in standard RPMI 1640/10% fetal bovine serum and stimulated with 1 μg/ml phytohemagglutinin (PHA) for 24 h. CD4+ T cells were then separated by positive selection using magnetic beads (Miltenyi Biotec), washed, and cultured for 72 h with 10 ng/ml IL-2 in either RPMI 1640/10% fetal bovine serum (FBS) or custom RPMI 1640 without vitamin B6, methionine (Met), folate, vitamin B12, riboflavin, and choline as the base. ..

Article Title: Cytosolic DNA-activated Human Dendritic Cells are Potent Activators of the Adaptive Immune Response #
Article Snippet: .. Human monocyte-derived DCs were generated from CD14+ blood monocytes isolated from peripheral blood mononuclear cells (PBMCs) separated from Buffy Coats by Ficoll-Paque (Fisher, Pittsburgh, PA) gradient centrifugation ( ) followed by positive selection with anti-CD14-coated magnetic beads (Miltenyi Biotech, Auburn, CA). .. Purified CD14+ monocytes (≥95%) were plated at 2×106 cell/ml concentration and cultured in RPMI 1640 medium (Sigma, St. Louis, MO) supplemented with 2 mM Glutamine, 100 U/ml penicillin and 100 g/ml streptomycin in the presence of 100 ng/ml IL-4 and 75 ng/ml GM-CSF (Peprotech, Rocky Hill, NJ) given on days 0 and 2.

Magnetic Beads:

Article Title: The Human Cytomegalovirus Lytic Cycle Is Induced by 1,25-Dihydroxyvitamin D3 in Peripheral Blood Monocytes and in the THP-1 Monocytic Cell Line
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) from healthy anonymous donors were isolated by Ficoll-paque and CD14 positive monocytes were subsequently isolated from PBMCs using CD14 magnetic beads (Miltenyi). .. Monocytes were treated with 1,25-dihydroxyvitamin D3 (100 nM) for 2 days and infected with HCMV TB40E at an MOI of 10.

Article Title: Oxidative Stress and Dietary Micronutrient Deficiencies Contribute to Overexpression of Epigenetically Regulated Genes by Lupus T Cells
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by density gradient centrifugation on Ficoll-Paque, cultured in standard RPMI 1640/10% fetal bovine serum and stimulated with 1 μg/ml phytohemagglutinin (PHA) for 24 h. CD4+ T cells were then separated by positive selection using magnetic beads (Miltenyi Biotec), washed, and cultured for 72 h with 10 ng/ml IL-2 in either RPMI 1640/10% fetal bovine serum (FBS) or custom RPMI 1640 without vitamin B6, methionine (Met), folate, vitamin B12, riboflavin, and choline as the base. ..

Article Title: Cytosolic DNA-activated Human Dendritic Cells are Potent Activators of the Adaptive Immune Response #
Article Snippet: .. Human monocyte-derived DCs were generated from CD14+ blood monocytes isolated from peripheral blood mononuclear cells (PBMCs) separated from Buffy Coats by Ficoll-Paque (Fisher, Pittsburgh, PA) gradient centrifugation ( ) followed by positive selection with anti-CD14-coated magnetic beads (Miltenyi Biotech, Auburn, CA). .. Purified CD14+ monocytes (≥95%) were plated at 2×106 cell/ml concentration and cultured in RPMI 1640 medium (Sigma, St. Louis, MO) supplemented with 2 mM Glutamine, 100 U/ml penicillin and 100 g/ml streptomycin in the presence of 100 ng/ml IL-4 and 75 ng/ml GM-CSF (Peprotech, Rocky Hill, NJ) given on days 0 and 2.

Isolation:

Article Title: Extended Freeze-Dried BCG Instructed pDCs Induce Suppressive Tregs and Dampen EAE
Article Snippet: .. PBMC from Buffy Coats were first isolated with Ficoll Paque and pDC purified with Diamond Plasmacytoid Dendritic Cell Isolation Kit II human according to the manufacturer (Miltenyi Biotec). .. For in vitro co-culture assay, purified pDCs (60 × 104 /200μl) were stimulated with 20μg/ml EFD BCG in complete RPMI medium for 16 h in presence of human IL-3 (10 ng/ml, PeproTech), extensively washed, and co-cultured with purified allogenic naïve CD4+ T cells (Naive CD4+ T Cell Isolation Kit II human, Miltenyi Biotec) at a 1/3 ratio (16'000 DC/50'000 T) for 6 days in triplicates.

Article Title: Siglec-7 engagement by GBS β-protein suppresses pyroptotic cell death of natural killer cells
Article Snippet: .. Peripheral blood mononuclear cells were isolated by Ficoll-Paque–based centrifugation, and NK cells were isolated by the Human NK Cell Enrichment Kit (Miltenyi) per manufacturer’s instructions. .. Streptococcus agalactiae also includes details on GBS strains and growth conditions, human NK cell isolation and exposure to GBS methods, ELISA conditions for binding assays and cytokine secretion analysis, flow cytometry procedures, confocal microscopy procedures, and all relevant antibody clones and other reagent details required to execute the experimental procedures referred in the text.

Article Title: The Human Cytomegalovirus Lytic Cycle Is Induced by 1,25-Dihydroxyvitamin D3 in Peripheral Blood Monocytes and in the THP-1 Monocytic Cell Line
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) from healthy anonymous donors were isolated by Ficoll-paque and CD14 positive monocytes were subsequently isolated from PBMCs using CD14 magnetic beads (Miltenyi). .. Monocytes were treated with 1,25-dihydroxyvitamin D3 (100 nM) for 2 days and infected with HCMV TB40E at an MOI of 10.

Article Title: Dipalmitoylphosphatidylcholine modulates inflammatory functions of monocytic cells independently of mitogen activated protein kinases
Article Snippet: .. Isolation of human monocytes from peripheral blood was performed by density centrifugation over Ficoll Paque® and negative selection of monocytes using midiMACS (Miltenyi Biotec, Camberley, U.K.). ..

Article Title: Oxidative Stress and Dietary Micronutrient Deficiencies Contribute to Overexpression of Epigenetically Regulated Genes by Lupus T Cells
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by density gradient centrifugation on Ficoll-Paque, cultured in standard RPMI 1640/10% fetal bovine serum and stimulated with 1 μg/ml phytohemagglutinin (PHA) for 24 h. CD4+ T cells were then separated by positive selection using magnetic beads (Miltenyi Biotec), washed, and cultured for 72 h with 10 ng/ml IL-2 in either RPMI 1640/10% fetal bovine serum (FBS) or custom RPMI 1640 without vitamin B6, methionine (Met), folate, vitamin B12, riboflavin, and choline as the base. ..

Article Title: Cytosolic DNA-activated Human Dendritic Cells are Potent Activators of the Adaptive Immune Response #
Article Snippet: .. Human monocyte-derived DCs were generated from CD14+ blood monocytes isolated from peripheral blood mononuclear cells (PBMCs) separated from Buffy Coats by Ficoll-Paque (Fisher, Pittsburgh, PA) gradient centrifugation ( ) followed by positive selection with anti-CD14-coated magnetic beads (Miltenyi Biotech, Auburn, CA). .. Purified CD14+ monocytes (≥95%) were plated at 2×106 cell/ml concentration and cultured in RPMI 1640 medium (Sigma, St. Louis, MO) supplemented with 2 mM Glutamine, 100 U/ml penicillin and 100 g/ml streptomycin in the presence of 100 ng/ml IL-4 and 75 ng/ml GM-CSF (Peprotech, Rocky Hill, NJ) given on days 0 and 2.

Centrifugation:

Article Title: Siglec-7 engagement by GBS β-protein suppresses pyroptotic cell death of natural killer cells
Article Snippet: .. Peripheral blood mononuclear cells were isolated by Ficoll-Paque–based centrifugation, and NK cells were isolated by the Human NK Cell Enrichment Kit (Miltenyi) per manufacturer’s instructions. .. Streptococcus agalactiae also includes details on GBS strains and growth conditions, human NK cell isolation and exposure to GBS methods, ELISA conditions for binding assays and cytokine secretion analysis, flow cytometry procedures, confocal microscopy procedures, and all relevant antibody clones and other reagent details required to execute the experimental procedures referred in the text.

Article Title: Dipalmitoylphosphatidylcholine modulates inflammatory functions of monocytic cells independently of mitogen activated protein kinases
Article Snippet: .. Isolation of human monocytes from peripheral blood was performed by density centrifugation over Ficoll Paque® and negative selection of monocytes using midiMACS (Miltenyi Biotec, Camberley, U.K.). ..

Cell Culture:

Article Title: Oxidative Stress and Dietary Micronutrient Deficiencies Contribute to Overexpression of Epigenetically Regulated Genes by Lupus T Cells
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by density gradient centrifugation on Ficoll-Paque, cultured in standard RPMI 1640/10% fetal bovine serum and stimulated with 1 μg/ml phytohemagglutinin (PHA) for 24 h. CD4+ T cells were then separated by positive selection using magnetic beads (Miltenyi Biotec), washed, and cultured for 72 h with 10 ng/ml IL-2 in either RPMI 1640/10% fetal bovine serum (FBS) or custom RPMI 1640 without vitamin B6, methionine (Met), folate, vitamin B12, riboflavin, and choline as the base. ..

Purification:

Article Title: Extended Freeze-Dried BCG Instructed pDCs Induce Suppressive Tregs and Dampen EAE
Article Snippet: .. PBMC from Buffy Coats were first isolated with Ficoll Paque and pDC purified with Diamond Plasmacytoid Dendritic Cell Isolation Kit II human according to the manufacturer (Miltenyi Biotec). .. For in vitro co-culture assay, purified pDCs (60 × 104 /200μl) were stimulated with 20μg/ml EFD BCG in complete RPMI medium for 16 h in presence of human IL-3 (10 ng/ml, PeproTech), extensively washed, and co-cultured with purified allogenic naïve CD4+ T cells (Naive CD4+ T Cell Isolation Kit II human, Miltenyi Biotec) at a 1/3 ratio (16'000 DC/50'000 T) for 6 days in triplicates.

Generated:

Article Title: Cytosolic DNA-activated Human Dendritic Cells are Potent Activators of the Adaptive Immune Response #
Article Snippet: .. Human monocyte-derived DCs were generated from CD14+ blood monocytes isolated from peripheral blood mononuclear cells (PBMCs) separated from Buffy Coats by Ficoll-Paque (Fisher, Pittsburgh, PA) gradient centrifugation ( ) followed by positive selection with anti-CD14-coated magnetic beads (Miltenyi Biotech, Auburn, CA). .. Purified CD14+ monocytes (≥95%) were plated at 2×106 cell/ml concentration and cultured in RPMI 1640 medium (Sigma, St. Louis, MO) supplemented with 2 mM Glutamine, 100 U/ml penicillin and 100 g/ml streptomycin in the presence of 100 ng/ml IL-4 and 75 ng/ml GM-CSF (Peprotech, Rocky Hill, NJ) given on days 0 and 2.

Gradient Centrifugation:

Article Title: mRNA PGC-1α levels in blood samples reliably correlates with its myocardial expression: study in patients undergoing cardiac surgery
Article Snippet: .. Preparation of mononuclear cells from blood samples Immediately before starting extracorporeal circulation, 6–8 mL of peripheral blood collected in EDTA vacutainers (BD) were used to isolate mononuclear cells by Ficoll density gradient centrifugation using Ficoll-Paque™ (Miltenyi Biotec, Surrey, United Kingdom), following the manufacturer’s instructions. .. Isolated cells were analyzed using Cytospin and Fast Panoptic Staining (Panreac, Barcelona, Spain).

Article Title: Oxidative Stress and Dietary Micronutrient Deficiencies Contribute to Overexpression of Epigenetically Regulated Genes by Lupus T Cells
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by density gradient centrifugation on Ficoll-Paque, cultured in standard RPMI 1640/10% fetal bovine serum and stimulated with 1 μg/ml phytohemagglutinin (PHA) for 24 h. CD4+ T cells were then separated by positive selection using magnetic beads (Miltenyi Biotec), washed, and cultured for 72 h with 10 ng/ml IL-2 in either RPMI 1640/10% fetal bovine serum (FBS) or custom RPMI 1640 without vitamin B6, methionine (Met), folate, vitamin B12, riboflavin, and choline as the base. ..

Article Title: Cytosolic DNA-activated Human Dendritic Cells are Potent Activators of the Adaptive Immune Response #
Article Snippet: .. Human monocyte-derived DCs were generated from CD14+ blood monocytes isolated from peripheral blood mononuclear cells (PBMCs) separated from Buffy Coats by Ficoll-Paque (Fisher, Pittsburgh, PA) gradient centrifugation ( ) followed by positive selection with anti-CD14-coated magnetic beads (Miltenyi Biotech, Auburn, CA). .. Purified CD14+ monocytes (≥95%) were plated at 2×106 cell/ml concentration and cultured in RPMI 1640 medium (Sigma, St. Louis, MO) supplemented with 2 mM Glutamine, 100 U/ml penicillin and 100 g/ml streptomycin in the presence of 100 ng/ml IL-4 and 75 ng/ml GM-CSF (Peprotech, Rocky Hill, NJ) given on days 0 and 2.

Magnetic Cell Separation:

Article Title: Assessment by miRNA microarray of an autologous cancer antigen‐pulsed adoptive immune ensemble cell therapy (AC‐ACT) approach; demonstrated induction of anti‐oncogenic and anti‐PD‐L1 miRNAs, et al. Assessment by miRNA microarray of an autologous cancer antigen‐pulsed adoptive immune ensemble cell therapy (AC‐ACT) approach; demonstrated induction of anti‐oncogenic and anti‐PD‐L1 miRNAs
Article Snippet: .. PBMC was separated with Ficoll‐Paque, and a buffy coat fraction was obtained, and CD14 + cells were separated by MACSprep CD14 MicroBeads, human (Miltenyi Biotec) in an ice‐cold MACS buffer. .. CD14+ cells were cultured for dendritic cells.

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    Miltenyi Biotec ficoll paque density gradient centrifugation
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    https://www.bioz.com/result/ficoll paque density gradient centrifugation/product/Miltenyi Biotec
    Average 92 stars, based on 10 article reviews
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    https://www.bioz.com/result/ficoll paque plus centrifugation/product/Miltenyi Biotec
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ficoll paque plus centrifugation - by Bioz Stars, 2020-07
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      Buy from Supplier

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    Miltenyi Biotec ficoll paque plus density centrifugation
    Ficoll Paque Plus Density Centrifugation, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque plus density centrifugation/product/Miltenyi Biotec
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ficoll paque plus density centrifugation - by Bioz Stars, 2020-07
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      Buy from Supplier

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    Miltenyi Biotec ficoll paque separation
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    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    ficoll paque separation - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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