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Lonza ficoll paque
Ficoll Paque, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ficoll paque/product/Lonza
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
ficoll paque - by Bioz Stars, 2020-07
92/100 stars

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Isolation:

Article Title: Macrophage-associated wound healing contributes to African green monkey SIV pathogenesis control
Article Snippet: .. Monocyte sorting and RNA sequencing AGM and RM PBMCs were isolated from EDTA-treated whole blood with dilution in sterile phosphate-buffered saline (PBS) and centrifugation for 30 min at 1850 RPM at 25 °C in Ficoll-Paque™ (Lonza) at a 3:2 ratio. .. The isolated buffy coat was washed with PBS and contaminating red blood cells lysed using an ammonium-chloride-potassium lysing buffer (Lonza) for 10 min before washing with PBS (10 min, 1800 RPM, 25 °C).

Article Title: Redirecting T-cell specificity to EGFR using mRNA to self-limit expression of chimeric antigen receptor
Article Snippet: .. PBMC (Gulf Coast Regional Blood Bank) isolated by Ficoll-Paque were thawed and rested in complete RPMI-1640 for 2 hours, suspended at 2×108 /mL in human T cell electroporation buffer (Lonza, cat# VPA-1002), then 100 µL of cell suspension was mixed with 15 µg transposon and 5 µg SB11 transposase in an electroporation cuvette and electroporated via Amaxa Nucleofector (Lonza) using program U-014 for unstimulated human T cells. .. Following electroporation, cells were immediately transferred to phenol-free RPMI supplemented with 20% heat-inactivated FBS (HyClone), and 2 mM Glutamax-100 (Gibco) to recover overnight.

Centrifugation:

Article Title: Macrophage-associated wound healing contributes to African green monkey SIV pathogenesis control
Article Snippet: .. Monocyte sorting and RNA sequencing AGM and RM PBMCs were isolated from EDTA-treated whole blood with dilution in sterile phosphate-buffered saline (PBS) and centrifugation for 30 min at 1850 RPM at 25 °C in Ficoll-Paque™ (Lonza) at a 3:2 ratio. .. The isolated buffy coat was washed with PBS and contaminating red blood cells lysed using an ammonium-chloride-potassium lysing buffer (Lonza) for 10 min before washing with PBS (10 min, 1800 RPM, 25 °C).

Article Title: Biochemical and Parasitological Studies on the Effect of hUCB-Selected CD34+ Progenitor/Stem Cells in Mice Infected with Schistosoma mansoni
Article Snippet: .. A density-gradient centrifugation at 800 g for 20 min at 20°C using Ficoll-paque (1.077 g/mL; Lonza Bioscience) was performed to collect the buffy coat layer according to the manufacturer’s protocol. ..

RNA Sequencing Assay:

Article Title: Macrophage-associated wound healing contributes to African green monkey SIV pathogenesis control
Article Snippet: .. Monocyte sorting and RNA sequencing AGM and RM PBMCs were isolated from EDTA-treated whole blood with dilution in sterile phosphate-buffered saline (PBS) and centrifugation for 30 min at 1850 RPM at 25 °C in Ficoll-Paque™ (Lonza) at a 3:2 ratio. .. The isolated buffy coat was washed with PBS and contaminating red blood cells lysed using an ammonium-chloride-potassium lysing buffer (Lonza) for 10 min before washing with PBS (10 min, 1800 RPM, 25 °C).

Electroporation:

Article Title: Redirecting T-cell specificity to EGFR using mRNA to self-limit expression of chimeric antigen receptor
Article Snippet: .. PBMC (Gulf Coast Regional Blood Bank) isolated by Ficoll-Paque were thawed and rested in complete RPMI-1640 for 2 hours, suspended at 2×108 /mL in human T cell electroporation buffer (Lonza, cat# VPA-1002), then 100 µL of cell suspension was mixed with 15 µg transposon and 5 µg SB11 transposase in an electroporation cuvette and electroporated via Amaxa Nucleofector (Lonza) using program U-014 for unstimulated human T cells. .. Following electroporation, cells were immediately transferred to phenol-free RPMI supplemented with 20% heat-inactivated FBS (HyClone), and 2 mM Glutamax-100 (Gibco) to recover overnight.

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  • 91
    Lonza ficoll paque gradient centrifugation
    Ficoll Paque Gradient Centrifugation, supplied by Lonza, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque gradient centrifugation/product/Lonza
    Average 91 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    ficoll paque gradient centrifugation - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    92
    Lonza ficoll paque
    Ficoll Paque, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque/product/Lonza
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ficoll paque - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    Lonza ficoll paque geheathcare
    Ficoll Paque Geheathcare, supplied by Lonza, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque geheathcare/product/Lonza
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ficoll paque geheathcare - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

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