Structured Review

Eurobio ficoll paque
Ficoll Paque, supplied by Eurobio, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ficoll paque/product/Eurobio
Average 92 stars, based on 12 article reviews
Price from $9.99 to $1999.99
ficoll paque - by Bioz Stars, 2020-07
92/100 stars

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Isolation:

Article Title: Inducing CTLA-4-dependent Immune Regulation by Selective CD28 blockade Promotes Regulatory T cells in Organ Transplantation
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by density centrifugation over Ficoll-Paque (Eurobio, Les Ulis, France). .. Freshly isolated PBMC were incubated with allogeneic irradiated PBMC (105 cells/well of each cell type) for 5 days at 37°C, 5% CO2 , in complete medium (RPMI 1640, 10% heat-inactivated allogeneic pooled sera, 2mM L-glutamine, 100 U/ml penicillin, 0.1 mg/ml streptomycin, 1% nonessential amino acids, 1mM sodium pyruvate and 5mM Hepes, all from Sigma).

Article Title: MHC class I diversity of olive baboons (Papio anubis) unravelled by next-generation sequencing
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by density centrifugation over Ficoll-Paque (Eurobio, Courtaboeuf, France), and sent to BPRC on dry ice. .. RNA was isolated at BPRC using the All prep DNA/RNA mini kit (QIAGEN Benelux B.V) following the manufacturer’s instructions. cDNA was produced from the isolated RNA using the RevertAid First Strand cDNA Kit (ThermoFisher Scientific) in accordance the manufacturer’s instructions.

Article Title: Anti-CD28 Antibody and Belatacept Exert Differential Effects on Mechanisms of Renal Allograft Rejection
Article Snippet: .. PBMCs were isolated from baboon whole blood by density centrifugation over Ficoll-Paque (Eurobio). .. Freshly isolated PBMCs were incubated with irradiated allogeneic PBMCs (105 cells per well of each cell type) for 5 days at 37°C and 5% CO2 in complete medium (RPMI 1640 [Gibco, Carlsbad, CA], 5% human or pooled baboon serum baboon serum is from our laboratory, 2 mM l -glutamine [Sigma-Aldrich], 100 U/ml penicillin [Sigma-Aldrich, St. Louis, MO], 0.1 mg/ml streptomycin [Sigma-Aldrich], 1% nonessential amino acids [Sigma-Aldrich], 1 mM sodium pyruvate [Sigma-Aldrich], and 5 mM Hepes [Sigma-Aldrich]).

Article Title: Association of KIR2DS1 and KIR2DS3 with fatal outcome in Ebola virus infection
Article Snippet: .. Genomic DNA was extracted using QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) from whole blood or PBMCs isolated using standard density gradient centrifugation with Ficoll-Paque (Eurobio, les Ulis, France). .. Genotyping was performed by polymerase chain reaction using the KIR typing kit (Miltenyi Biotec, Inc., Auburn, CA, USA) following manufacturer's instructions.

Article Title: Decreased Numbers of Blood Dendritic Cells and Defective Function of Regulatory T Cells in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis
Article Snippet: .. CD4+ CD25− , CD4+ CD25high CD127low/− Cell sorting PBMCs were isolated from EDTA whole blood by density centrifugation over Ficoll-Paque (Eurobio). .. 45×106 freshly isolated PBMC were incubated for 10 min in 100 µL of PBS- EDTA with anti-CD4 FITC (clone RPA-T4 BD biosciences), CD127 PE (clone SB/199 BD biosciences) and anti-CD25 AlexaFluor 647 (in house clone 33B3.1 conjugated to AF647 using a kit from Invitrogen).

Centrifugation:

Article Title: Identification and Isolation of Brucella suis Virulence Genes Involved in Resistance to the Human Innate Immune System ▿
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) from healthy donors were prepared by density centrifugation on Ficoll-Paque (Eurobio, Les Ulis, France). .. Monocytes were purified from PBMC based on their adherence properties.

Article Title: Inducing CTLA-4-dependent Immune Regulation by Selective CD28 blockade Promotes Regulatory T cells in Organ Transplantation
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by density centrifugation over Ficoll-Paque (Eurobio, Les Ulis, France). .. Freshly isolated PBMC were incubated with allogeneic irradiated PBMC (105 cells/well of each cell type) for 5 days at 37°C, 5% CO2 , in complete medium (RPMI 1640, 10% heat-inactivated allogeneic pooled sera, 2mM L-glutamine, 100 U/ml penicillin, 0.1 mg/ml streptomycin, 1% nonessential amino acids, 1mM sodium pyruvate and 5mM Hepes, all from Sigma).

Article Title: IL-21 promotes the development of a CD73-positive Vγ9Vδ2 T cell regulatory population
Article Snippet: .. PBMC were obtained by density centrifugation on Ficoll-Paque (Eurobio, Les Ulis, France) from the blood of healthy donors provided by the Etablissement Français du Sang (EFS). .. CD14+ monocytes were purified from PBMC by magnetic positive separation using magnetic beads (Miltenyi Biotec).

Article Title: MHC class I diversity of olive baboons (Papio anubis) unravelled by next-generation sequencing
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by density centrifugation over Ficoll-Paque (Eurobio, Courtaboeuf, France), and sent to BPRC on dry ice. .. RNA was isolated at BPRC using the All prep DNA/RNA mini kit (QIAGEN Benelux B.V) following the manufacturer’s instructions. cDNA was produced from the isolated RNA using the RevertAid First Strand cDNA Kit (ThermoFisher Scientific) in accordance the manufacturer’s instructions.

Article Title: Impairment of Intramacrophagic Brucella suis Multiplication by Human Natural Killer Cells through a Contact-Dependent Mechanism
Article Snippet: .. Peripheral blood mononuclear cells from healthy donors were prepared by density centrifugation on Ficoll-Paque (Eurobio, Les Ulis, France). .. Monocytes were purified from peripheral blood mononuclear cells by adherence ( ).

Article Title: Anti-CD28 Antibody and Belatacept Exert Differential Effects on Mechanisms of Renal Allograft Rejection
Article Snippet: .. PBMCs were isolated from baboon whole blood by density centrifugation over Ficoll-Paque (Eurobio). .. Freshly isolated PBMCs were incubated with irradiated allogeneic PBMCs (105 cells per well of each cell type) for 5 days at 37°C and 5% CO2 in complete medium (RPMI 1640 [Gibco, Carlsbad, CA], 5% human or pooled baboon serum baboon serum is from our laboratory, 2 mM l -glutamine [Sigma-Aldrich], 100 U/ml penicillin [Sigma-Aldrich, St. Louis, MO], 0.1 mg/ml streptomycin [Sigma-Aldrich], 1% nonessential amino acids [Sigma-Aldrich], 1 mM sodium pyruvate [Sigma-Aldrich], and 5 mM Hepes [Sigma-Aldrich]).

Article Title: Decreased Numbers of Blood Dendritic Cells and Defective Function of Regulatory T Cells in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis
Article Snippet: .. CD4+ CD25− , CD4+ CD25high CD127low/− Cell sorting PBMCs were isolated from EDTA whole blood by density centrifugation over Ficoll-Paque (Eurobio). .. 45×106 freshly isolated PBMC were incubated for 10 min in 100 µL of PBS- EDTA with anti-CD4 FITC (clone RPA-T4 BD biosciences), CD127 PE (clone SB/199 BD biosciences) and anti-CD25 AlexaFluor 647 (in house clone 33B3.1 conjugated to AF647 using a kit from Invitrogen).

Gradient Centrifugation:

Article Title: Association of KIR2DS1 and KIR2DS3 with fatal outcome in Ebola virus infection
Article Snippet: .. Genomic DNA was extracted using QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) from whole blood or PBMCs isolated using standard density gradient centrifugation with Ficoll-Paque (Eurobio, les Ulis, France). .. Genotyping was performed by polymerase chain reaction using the KIR typing kit (Miltenyi Biotec, Inc., Auburn, CA, USA) following manufacturer's instructions.

FACS:

Article Title: Decreased Numbers of Blood Dendritic Cells and Defective Function of Regulatory T Cells in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis
Article Snippet: .. CD4+ CD25− , CD4+ CD25high CD127low/− Cell sorting PBMCs were isolated from EDTA whole blood by density centrifugation over Ficoll-Paque (Eurobio). .. 45×106 freshly isolated PBMC were incubated for 10 min in 100 µL of PBS- EDTA with anti-CD4 FITC (clone RPA-T4 BD biosciences), CD127 PE (clone SB/199 BD biosciences) and anti-CD25 AlexaFluor 647 (in house clone 33B3.1 conjugated to AF647 using a kit from Invitrogen).

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  • 92
    Eurobio ficoll paque
    Ficoll Paque, supplied by Eurobio, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque/product/Eurobio
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    ficoll paque - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    89
    Eurobio ficoll paque density centrifugation
    Ficoll Paque Density Centrifugation, supplied by Eurobio, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque density centrifugation/product/Eurobio
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ficoll paque density centrifugation - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    90
    Eurobio ficoll paque plus density gradient centrifugation
    Ficoll Paque Plus Density Gradient Centrifugation, supplied by Eurobio, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque plus density gradient centrifugation/product/Eurobio
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ficoll paque plus density gradient centrifugation - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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