Structured Review

Cedarlane ficoll paque
Ficoll Paque, supplied by Cedarlane, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ficoll paque/product/Cedarlane
Average 92 stars, based on 8 article reviews
Price from $9.99 to $1999.99
ficoll paque - by Bioz Stars, 2020-07
92/100 stars

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Isolation:

Article Title: γ-H2AX/53BP1/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes
Article Snippet: .. HPBLs were isolated by density gradient centrifugation in Ficoll-Paque (Cedarlane Co., Burlington, Ontario, Canada) according to the manufacturer’s instructions. ..

Article Title: Molecular features of the complementarity determining region 3 motif of the T cell population and subsets in the blood of patients with chronic severe hepatitis B
Article Snippet: .. PBMCs from 42 CSHB patients were isolated from fresh EDTAK2 anti-coagulated blood using Ficoll-Paque (CEDARLANE, Netherlands) density gradient centrifugation. .. For 15 samples, CD8+ cells were positively selected from PBMCs using anti-CD8 antibody-coated magnetic beads according to the directions of the manufacturer (Dynal Biotech, Norway), and the remaining fraction was depleted of CD8+ cells (CD8- cells).

Article Title: Molecular characterization of T cell receptor beta variable in the peripheral blood T cell repertoire in subjects with active tuberculosis or latent tuberculosis infection
Article Snippet: .. Isolation of PBMCs, CD4+ and CD8+ T cell sorted Peripheral blood mononuclear cells (PBMCs) from 42 subjects with active TB or LTBI were obtained from fresh heparinized blood by density gradient centrifugation on Ficoll-Paque (CEDARLANE, Netherlands). .. CD4+ and CD8+ T cells were positively selected from PBMCs using anti-CD4/anti-CD8 monoclonal antibodies coupled to antibody-coated magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s instructions.

Article Title: Induction and inhibition of the pan-nuclear gamma-H2AX response in resting human peripheral blood lymphocytes after X-ray irradiation
Article Snippet: .. Lymphocytes were isolated from blood using density gradient centrifugation in Ficoll-Paque (Cedarlane Co., Burlington, ON, Canada) according to the manufacturer’s instructions. .. Isolated HPBLs at a density of 1×106 cell/ml were cultured in RPMI 1640 medium (Gibco, Invitrogen Technologies, Carlsbad, CA, USA) supplemented with 15% fetal calf serum (Invitrogen Technologies), 5% (v/v) plasma, 100 U/ml penicillin and 100 μ g/ml streptomycin at 5% CO2 and 37°C in a humidified incubator.

Centrifugation:

Article Title: In Situ Patrolling of Regulatory T Cells Is Essential for Protecting Autoimmune Exocrinopathy
Article Snippet: .. After various tissues were cut into small pieces, digested by collagenase (500 U/ml) and hyaluronidase (300 U/ml), and homogenized, the lymphocytes were purified by centrifugation with ficoll-paque (CEDARLANE Laboratories LtD., Burlington, NC). ..

Gradient Centrifugation:

Article Title: γ-H2AX/53BP1/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes
Article Snippet: .. HPBLs were isolated by density gradient centrifugation in Ficoll-Paque (Cedarlane Co., Burlington, Ontario, Canada) according to the manufacturer’s instructions. ..

Article Title: Molecular features of the complementarity determining region 3 motif of the T cell population and subsets in the blood of patients with chronic severe hepatitis B
Article Snippet: .. PBMCs from 42 CSHB patients were isolated from fresh EDTAK2 anti-coagulated blood using Ficoll-Paque (CEDARLANE, Netherlands) density gradient centrifugation. .. For 15 samples, CD8+ cells were positively selected from PBMCs using anti-CD8 antibody-coated magnetic beads according to the directions of the manufacturer (Dynal Biotech, Norway), and the remaining fraction was depleted of CD8+ cells (CD8- cells).

Article Title: Molecular characterization of T cell receptor beta variable in the peripheral blood T cell repertoire in subjects with active tuberculosis or latent tuberculosis infection
Article Snippet: .. Isolation of PBMCs, CD4+ and CD8+ T cell sorted Peripheral blood mononuclear cells (PBMCs) from 42 subjects with active TB or LTBI were obtained from fresh heparinized blood by density gradient centrifugation on Ficoll-Paque (CEDARLANE, Netherlands). .. CD4+ and CD8+ T cells were positively selected from PBMCs using anti-CD4/anti-CD8 monoclonal antibodies coupled to antibody-coated magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s instructions.

Article Title: Induction and inhibition of the pan-nuclear gamma-H2AX response in resting human peripheral blood lymphocytes after X-ray irradiation
Article Snippet: .. Lymphocytes were isolated from blood using density gradient centrifugation in Ficoll-Paque (Cedarlane Co., Burlington, ON, Canada) according to the manufacturer’s instructions. .. Isolated HPBLs at a density of 1×106 cell/ml were cultured in RPMI 1640 medium (Gibco, Invitrogen Technologies, Carlsbad, CA, USA) supplemented with 15% fetal calf serum (Invitrogen Technologies), 5% (v/v) plasma, 100 U/ml penicillin and 100 μ g/ml streptomycin at 5% CO2 and 37°C in a humidified incubator.

Purification:

Article Title: In Situ Patrolling of Regulatory T Cells Is Essential for Protecting Autoimmune Exocrinopathy
Article Snippet: .. After various tissues were cut into small pieces, digested by collagenase (500 U/ml) and hyaluronidase (300 U/ml), and homogenized, the lymphocytes were purified by centrifugation with ficoll-paque (CEDARLANE Laboratories LtD., Burlington, NC). ..

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    Cedarlane ficoll paque density separation
    Ficoll Paque Density Separation, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque density separation/product/Cedarlane
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    Cedarlane ficoll paque plus
    Ficoll Paque Plus, supplied by Cedarlane, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque plus/product/Cedarlane
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    Price from $9.99 to $1999.99
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