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Biochrom ficoll paque
Ficoll Paque, supplied by Biochrom, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ficoll paque/product/Biochrom
Average 93 stars, based on 26 article reviews
Price from $9.99 to $1999.99
ficoll paque - by Bioz Stars, 2020-07
93/100 stars

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Centrifugation:

Article Title: Dose assessment inter-comparisons within the RENEB network using G0-lymphocyte prematurely condensed chromosomes (PCC assay)
Article Snippet: .. The peripheral blood was always sampled in heparinized vials and, for the isolation of lymphocytes, whole blood was carefully layered on top of equal amount of Ficoll-Paque or Biocoll gradient (Biochrom) in a test tube before centrifugation. .. In order to quantitate radiation exposure by means of initially induced PCC fragments, the PCC methodology was applied as soon as possible, since the initial radiation-induced fragments in excess of 46 PCCs decrease with time ( , ).

Article Title: Nitration of Wheat Amylase Trypsin Inhibitors Increases Their Innate and Adaptive Immunostimulatory Potential in vitro
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Paque 1.077 g/mL (Biochrom) density centrifugation. ..

Isolation:

Article Title: Dose assessment inter-comparisons within the RENEB network using G0-lymphocyte prematurely condensed chromosomes (PCC assay)
Article Snippet: .. The peripheral blood was always sampled in heparinized vials and, for the isolation of lymphocytes, whole blood was carefully layered on top of equal amount of Ficoll-Paque or Biocoll gradient (Biochrom) in a test tube before centrifugation. .. In order to quantitate radiation exposure by means of initially induced PCC fragments, the PCC methodology was applied as soon as possible, since the initial radiation-induced fragments in excess of 46 PCCs decrease with time ( , ).

Article Title: The effect of interleukin (IL)‐21 and CD4+CD25++ T cells on cytokine production of CD4+ responder T cells in patients with myasthenia gravis
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from ethylenediamine tetraacetic acid (EDTA) blood samples by density gradient centrifugation using Ficoll‐Paque (Histopaque‐1077; Biochrom, Cambridge, UK) at 1000 × g for 30 min. .. The acquired PBMCs were washed twice with phosphate‐buffered saline (PBS) and stored in a cell culture medium.

Article Title: Nitration of Wheat Amylase Trypsin Inhibitors Increases Their Innate and Adaptive Immunostimulatory Potential in vitro
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Paque 1.077 g/mL (Biochrom) density centrifugation. ..

Article Title: The transcription factor MITF is a critical regulator of GPNMB expression in dendritic cells
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll/Paque (Biochrom, Berlin, Germany) density gradient centrifugation of buffy coats obtained from healthy volunteers (Blood Donation Center, University of Tübingen). .. For plastic adherence cells were seeded (1 × 108 /10 mL) into 75 cm2 cell culture flasks (Corning, Cambridge, MA, USA) in serum-free X-VIVO 20 medium (Cambrex Bio Science, Verviers, Belgium).

Article Title: Efficacy of different hemodialysis methods on dendritic cell marker CD40 and CD80 and platelet activation marker CD62P and P10 in patients with chronic renal failure. Efficacy of different hemodialysis methods on dendritic cell marker CD40 and CD80 and platelet activation marker CD62P and P10 in patients with chronic renal failure
Article Snippet: .. Mononuclear cells were isolated using a Ficoll‐Paque (Biochrom, Berlin, Germany) density gradient centrifugation, seeded into a 6‐well plate at a density of 1 × 106 cells/cm2 , and cultured in a 5% CO2 incubator at 37°C after the addition of Royal Park Memorial Institute 1640 medium containing 10% fetal calf serum (FCS). .. Four hours later, the nonadherent cells were discarded, while the adherent cells were further cultured with the addition of FM‐CSF (100 ng/mL) and IL‐4 (50 ng/mL) with daily solution replacement.

Article Title: M2 macrophage-mediated interleukin-4 signalling induces myofibroblast phenotype during the progression of benign prostatic hyperplasia
Article Snippet: .. Additionally, human peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood of patients with BPH, using Ficoll-Paque (Biochrom, Berlin, Germany) density gradient centrifugation. .. Human monocytes were isolated via flow cytometry with a CD14-FITC antibody (BD Biosciences, CA, USA), and treated with 1000 units/ml rh GM-CSF (PeproTech) for 7 d to obtain macrophages (Supplementary Figure ).

Article Title: Infiltrating CD4+ T cells attenuate chemotherapy sensitivity in prostate cancer via CCL5 signaling, et al. Infiltrating CD4+ T cells attenuate chemotherapy sensitivity in prostate cancer via CCL5 signaling
Article Snippet: .. 2.10 Isolation of CD4+ T cells and flow cytometry Human peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood of healthy patients, using Ficoll‐Paque (Biochrom, Berlin, Germany) density gradient centrifugation. .. Then PBMCs incubated with CD4‐APC antibody (BD Biosciences, CA) for 20 minutes.

Flow Cytometry:

Article Title: Infiltrating CD4+ T cells attenuate chemotherapy sensitivity in prostate cancer via CCL5 signaling, et al. Infiltrating CD4+ T cells attenuate chemotherapy sensitivity in prostate cancer via CCL5 signaling
Article Snippet: .. 2.10 Isolation of CD4+ T cells and flow cytometry Human peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood of healthy patients, using Ficoll‐Paque (Biochrom, Berlin, Germany) density gradient centrifugation. .. Then PBMCs incubated with CD4‐APC antibody (BD Biosciences, CA) for 20 minutes.

Cell Culture:

Article Title: Efficacy of different hemodialysis methods on dendritic cell marker CD40 and CD80 and platelet activation marker CD62P and P10 in patients with chronic renal failure. Efficacy of different hemodialysis methods on dendritic cell marker CD40 and CD80 and platelet activation marker CD62P and P10 in patients with chronic renal failure
Article Snippet: .. Mononuclear cells were isolated using a Ficoll‐Paque (Biochrom, Berlin, Germany) density gradient centrifugation, seeded into a 6‐well plate at a density of 1 × 106 cells/cm2 , and cultured in a 5% CO2 incubator at 37°C after the addition of Royal Park Memorial Institute 1640 medium containing 10% fetal calf serum (FCS). .. Four hours later, the nonadherent cells were discarded, while the adherent cells were further cultured with the addition of FM‐CSF (100 ng/mL) and IL‐4 (50 ng/mL) with daily solution replacement.

Cytometry:

Article Title: Infiltrating CD4+ T cells attenuate chemotherapy sensitivity in prostate cancer via CCL5 signaling, et al. Infiltrating CD4+ T cells attenuate chemotherapy sensitivity in prostate cancer via CCL5 signaling
Article Snippet: .. 2.10 Isolation of CD4+ T cells and flow cytometry Human peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood of healthy patients, using Ficoll‐Paque (Biochrom, Berlin, Germany) density gradient centrifugation. .. Then PBMCs incubated with CD4‐APC antibody (BD Biosciences, CA) for 20 minutes.

Gradient Centrifugation:

Article Title: The effect of interleukin (IL)‐21 and CD4+CD25++ T cells on cytokine production of CD4+ responder T cells in patients with myasthenia gravis
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from ethylenediamine tetraacetic acid (EDTA) blood samples by density gradient centrifugation using Ficoll‐Paque (Histopaque‐1077; Biochrom, Cambridge, UK) at 1000 × g for 30 min. .. The acquired PBMCs were washed twice with phosphate‐buffered saline (PBS) and stored in a cell culture medium.

Article Title: The transcription factor MITF is a critical regulator of GPNMB expression in dendritic cells
Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll/Paque (Biochrom, Berlin, Germany) density gradient centrifugation of buffy coats obtained from healthy volunteers (Blood Donation Center, University of Tübingen). .. For plastic adherence cells were seeded (1 × 108 /10 mL) into 75 cm2 cell culture flasks (Corning, Cambridge, MA, USA) in serum-free X-VIVO 20 medium (Cambrex Bio Science, Verviers, Belgium).

Article Title: Efficacy of different hemodialysis methods on dendritic cell marker CD40 and CD80 and platelet activation marker CD62P and P10 in patients with chronic renal failure. Efficacy of different hemodialysis methods on dendritic cell marker CD40 and CD80 and platelet activation marker CD62P and P10 in patients with chronic renal failure
Article Snippet: .. Mononuclear cells were isolated using a Ficoll‐Paque (Biochrom, Berlin, Germany) density gradient centrifugation, seeded into a 6‐well plate at a density of 1 × 106 cells/cm2 , and cultured in a 5% CO2 incubator at 37°C after the addition of Royal Park Memorial Institute 1640 medium containing 10% fetal calf serum (FCS). .. Four hours later, the nonadherent cells were discarded, while the adherent cells were further cultured with the addition of FM‐CSF (100 ng/mL) and IL‐4 (50 ng/mL) with daily solution replacement.

Article Title: Mechanical Stress Downregulates MHC Class I Expression on Human Cancer Cell Membrane
Article Snippet: .. Peripheral Blood Lymphocytes (PBLs) were obtained from healthy donors by Ficoll-Paque (Biochrom AG, Berlin, Germany) density gradient centrifugation the human samples were collected accordingly with the Ethics Committee of University Magna Graecia of Catanzaro (49/2003). .. Cells were grown in RPMI 1640 medium and in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% of fetal bovine serum (FBS) (SIGMA Aldrich, St. Louis) and penicillin (100 IU/mL) and streptomycin (100 mg/mL) (SIGMA Aldrich, St. Louis) and were maintained at 37°C in a humidified 5% CO2 atmosphere.

Article Title: M2 macrophage-mediated interleukin-4 signalling induces myofibroblast phenotype during the progression of benign prostatic hyperplasia
Article Snippet: .. Additionally, human peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood of patients with BPH, using Ficoll-Paque (Biochrom, Berlin, Germany) density gradient centrifugation. .. Human monocytes were isolated via flow cytometry with a CD14-FITC antibody (BD Biosciences, CA, USA), and treated with 1000 units/ml rh GM-CSF (PeproTech) for 7 d to obtain macrophages (Supplementary Figure ).

Article Title: Infiltrating CD4+ T cells attenuate chemotherapy sensitivity in prostate cancer via CCL5 signaling, et al. Infiltrating CD4+ T cells attenuate chemotherapy sensitivity in prostate cancer via CCL5 signaling
Article Snippet: .. 2.10 Isolation of CD4+ T cells and flow cytometry Human peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood of healthy patients, using Ficoll‐Paque (Biochrom, Berlin, Germany) density gradient centrifugation. .. Then PBMCs incubated with CD4‐APC antibody (BD Biosciences, CA) for 20 minutes.

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  • 90
    Biochrom ficoll paque gradient centrifugation
    Ficoll Paque Gradient Centrifugation, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque gradient centrifugation/product/Biochrom
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ficoll paque gradient centrifugation - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    85
    Biochrom ficoll paque 1 077
    Ficoll Paque 1 077, supplied by Biochrom, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque 1 077/product/Biochrom
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ficoll paque 1 077 - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    93
    Biochrom ficoll paque
    Ficoll Paque, supplied by Biochrom, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque/product/Biochrom
    Average 93 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    ficoll paque - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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