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Becton Dickinson ficoll paque
Ficoll Paque, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ficoll paque/product/Becton Dickinson
Average 92 stars, based on 8 article reviews
Price from $9.99 to $1999.99
ficoll paque - by Bioz Stars, 2020-07
92/100 stars

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Irradiation:

Article Title: Cellular immunotherapy on primary multiple myeloma expanded in a 3D bone marrow niche model
Article Snippet: .. Endothelial progenitor cells (EPCs) were isolated from cord blood by density-gradient centrifugation of MNCs using Ficoll-paque, seeded on collagen I (BD Biosciences)-coated wells and expanded in EGM-2 medium (Lonza, Switzerland), SingleQuots™ Kit (Lonza, Switzerland), 10% (v/v) FBS, 100 U/ml penicillin and 100 µg/ml streptomycin. αβT cells from both healthy donors and myeloma patients were retrovirally transduced into TEGs with engineered T cell receptors (TCRs) using the Vγ9Vδ2-TCR clone G115 as described earlier., The transduced T cells were stimulated biweekly with 1 μg/ml PHA-L (Sigma-Aldrich, USA), 50 U/ml IL-2 (Novartis Pharma, Switzerland), 5 ng/ml IL-15 (R & D Systems, USA), and irradiated allogeneic PBMCs, Daudi and LCL-TM cell lines. .. Mock αβT cells were transduced with a non-functional γδTCR (γ9-G115LM1/δ2-G115 wt).

Centrifugation:

Article Title: Cellular immunotherapy on primary multiple myeloma expanded in a 3D bone marrow niche model
Article Snippet: .. Endothelial progenitor cells (EPCs) were isolated from cord blood by density-gradient centrifugation of MNCs using Ficoll-paque, seeded on collagen I (BD Biosciences)-coated wells and expanded in EGM-2 medium (Lonza, Switzerland), SingleQuots™ Kit (Lonza, Switzerland), 10% (v/v) FBS, 100 U/ml penicillin and 100 µg/ml streptomycin. αβT cells from both healthy donors and myeloma patients were retrovirally transduced into TEGs with engineered T cell receptors (TCRs) using the Vγ9Vδ2-TCR clone G115 as described earlier., The transduced T cells were stimulated biweekly with 1 μg/ml PHA-L (Sigma-Aldrich, USA), 50 U/ml IL-2 (Novartis Pharma, Switzerland), 5 ng/ml IL-15 (R & D Systems, USA), and irradiated allogeneic PBMCs, Daudi and LCL-TM cell lines. .. Mock αβT cells were transduced with a non-functional γδTCR (γ9-G115LM1/δ2-G115 wt).

Article Title: Rheumatoid factor isotype switch and somatic mutation variants within rheumatoid arthritis synovium
Article Snippet: .. Synovial mononuclear cells (MNCs) were separated by centrifugation on Ficoll–Paque of 70-μm nylon mesh (Beckton-Dickinson, Oxford, UK) -filtered cells from collagenase (5 mg/ml) -digested diced synovium. .. Monocytes and natural killer (NK) cells were eliminated by 35-min incubation with l-leucine methyl ester (5 mm) (Sigma, Poole, UK).

Isolation:

Article Title: Aberrant Expression of Intracellular let-7e, miR-146a, and miR-155 Correlates with Severity of Depression in Patients with Major Depressive Disorder and Is Ameliorated after Antidepressant Treatment
Article Snippet: .. Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR) Venous blood (15 mL) samples were drawn between 6:00 a.m. and 10:00 a.m., after subjects fasted for 9 h. PBMCs were isolated from venous blood samples by Ficoll–Paque (GE, #17-5442-02) density gradient centrifugation and labeled with BD IMag anti-human CD14 Magnetic Particles DM (BD Biosciences, #557769) according to the manufacturer’s directions. .. RNA samples were extracted using Quick-RNA MiniPrep kits (Zymo Research R1055) and reverse-transcribed to complementary DNA (cDNA) using TaqMan Micro RNA Transcription Kits (Applied Biosystems 4366596).

Article Title: Cellular immunotherapy on primary multiple myeloma expanded in a 3D bone marrow niche model
Article Snippet: .. Endothelial progenitor cells (EPCs) were isolated from cord blood by density-gradient centrifugation of MNCs using Ficoll-paque, seeded on collagen I (BD Biosciences)-coated wells and expanded in EGM-2 medium (Lonza, Switzerland), SingleQuots™ Kit (Lonza, Switzerland), 10% (v/v) FBS, 100 U/ml penicillin and 100 µg/ml streptomycin. αβT cells from both healthy donors and myeloma patients were retrovirally transduced into TEGs with engineered T cell receptors (TCRs) using the Vγ9Vδ2-TCR clone G115 as described earlier., The transduced T cells were stimulated biweekly with 1 μg/ml PHA-L (Sigma-Aldrich, USA), 50 U/ml IL-2 (Novartis Pharma, Switzerland), 5 ng/ml IL-15 (R & D Systems, USA), and irradiated allogeneic PBMCs, Daudi and LCL-TM cell lines. .. Mock αβT cells were transduced with a non-functional γδTCR (γ9-G115LM1/δ2-G115 wt).

Article Title: Allogeneic Mesenchymal Stem Cells Restore Endothelial Function in Heart Failure by Stimulating Endothelial Progenitor Cells
Article Snippet: .. EPCs were isolated from samples using Ficoll-Paque and five million cells were seeded on 6-well fibronectin-coated dishes (BD biosciences) in CFU-Hill medium (stem cell technologies, cat#05900) ( ). ..

Article Title: Extracellular MicroRNA Signature of Human Helper T Cell Subsets in Health and Autoimmunity *
Article Snippet: .. Lymphocytes were isolated from buffy coats, obtained from the blood of healthy donors by Ficoll-Paque, and purified by sorting on a FACSAria III (BD) by specific markers of naïve Treg cells (CD4+ , CD45RO− , CD62L+ , CD25+ , CD127dim ). .. Then, purified cells were expanded using anti-CD3 (30 ng/ml, OKT3 clone), IL-2 (200 units/ml), irradiated peripheral blood mononuclear cells as feeders, and irradiated EBV cell lines.

Article Title: Pilot trial of interleukin-2 and zoledronic acid to augment γδ T cells as treatment for patients with refractory renal cell carcinoma
Article Snippet: .. PBMC were isolated by density gradient centrifugation over Ficoll-Paque using standard techniques and stained with the following fluorochrome-labeled monoclonal antibodies for respective experiments: V γ 9-FITC, V δ 2-PE, CD3-FITC, αβ TCR-PE, CD4-APC, CD8-PE, V δ 2-FITC, CD27-PE, CD45RA-APC (Beckton Dickinson (BD) Bioscience Pharmingen, San Jose, CA), or isotype controls (IgG1-FITC, IgG1-PE (Sigma, St. Louis, MO), IgG1-APC (BD)). .. After labeling, the cells were washed and fixed with paraformaldehyde (Sigma, St. Louis, MO) and the samples analyzed on a FACSCalibur (Becton–Dickinson Biosciences, Mountain View, CA).

Quantitative RT-PCR:

Article Title: Aberrant Expression of Intracellular let-7e, miR-146a, and miR-155 Correlates with Severity of Depression in Patients with Major Depressive Disorder and Is Ameliorated after Antidepressant Treatment
Article Snippet: .. Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR) Venous blood (15 mL) samples were drawn between 6:00 a.m. and 10:00 a.m., after subjects fasted for 9 h. PBMCs were isolated from venous blood samples by Ficoll–Paque (GE, #17-5442-02) density gradient centrifugation and labeled with BD IMag anti-human CD14 Magnetic Particles DM (BD Biosciences, #557769) according to the manufacturer’s directions. .. RNA samples were extracted using Quick-RNA MiniPrep kits (Zymo Research R1055) and reverse-transcribed to complementary DNA (cDNA) using TaqMan Micro RNA Transcription Kits (Applied Biosystems 4366596).

Purification:

Article Title: Extracellular MicroRNA Signature of Human Helper T Cell Subsets in Health and Autoimmunity *
Article Snippet: .. Lymphocytes were isolated from buffy coats, obtained from the blood of healthy donors by Ficoll-Paque, and purified by sorting on a FACSAria III (BD) by specific markers of naïve Treg cells (CD4+ , CD45RO− , CD62L+ , CD25+ , CD127dim ). .. Then, purified cells were expanded using anti-CD3 (30 ng/ml, OKT3 clone), IL-2 (200 units/ml), irradiated peripheral blood mononuclear cells as feeders, and irradiated EBV cell lines.

Labeling:

Article Title: Aberrant Expression of Intracellular let-7e, miR-146a, and miR-155 Correlates with Severity of Depression in Patients with Major Depressive Disorder and Is Ameliorated after Antidepressant Treatment
Article Snippet: .. Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR) Venous blood (15 mL) samples were drawn between 6:00 a.m. and 10:00 a.m., after subjects fasted for 9 h. PBMCs were isolated from venous blood samples by Ficoll–Paque (GE, #17-5442-02) density gradient centrifugation and labeled with BD IMag anti-human CD14 Magnetic Particles DM (BD Biosciences, #557769) according to the manufacturer’s directions. .. RNA samples were extracted using Quick-RNA MiniPrep kits (Zymo Research R1055) and reverse-transcribed to complementary DNA (cDNA) using TaqMan Micro RNA Transcription Kits (Applied Biosystems 4366596).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Aberrant Expression of Intracellular let-7e, miR-146a, and miR-155 Correlates with Severity of Depression in Patients with Major Depressive Disorder and Is Ameliorated after Antidepressant Treatment
Article Snippet: .. Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR) Venous blood (15 mL) samples were drawn between 6:00 a.m. and 10:00 a.m., after subjects fasted for 9 h. PBMCs were isolated from venous blood samples by Ficoll–Paque (GE, #17-5442-02) density gradient centrifugation and labeled with BD IMag anti-human CD14 Magnetic Particles DM (BD Biosciences, #557769) according to the manufacturer’s directions. .. RNA samples were extracted using Quick-RNA MiniPrep kits (Zymo Research R1055) and reverse-transcribed to complementary DNA (cDNA) using TaqMan Micro RNA Transcription Kits (Applied Biosystems 4366596).

Staining:

Article Title: Pilot trial of interleukin-2 and zoledronic acid to augment γδ T cells as treatment for patients with refractory renal cell carcinoma
Article Snippet: .. PBMC were isolated by density gradient centrifugation over Ficoll-Paque using standard techniques and stained with the following fluorochrome-labeled monoclonal antibodies for respective experiments: V γ 9-FITC, V δ 2-PE, CD3-FITC, αβ TCR-PE, CD4-APC, CD8-PE, V δ 2-FITC, CD27-PE, CD45RA-APC (Beckton Dickinson (BD) Bioscience Pharmingen, San Jose, CA), or isotype controls (IgG1-FITC, IgG1-PE (Sigma, St. Louis, MO), IgG1-APC (BD)). .. After labeling, the cells were washed and fixed with paraformaldehyde (Sigma, St. Louis, MO) and the samples analyzed on a FACSCalibur (Becton–Dickinson Biosciences, Mountain View, CA).

Chloramphenicol Acetyltransferase Assay:

Article Title: Allogeneic Mesenchymal Stem Cells Restore Endothelial Function in Heart Failure by Stimulating Endothelial Progenitor Cells
Article Snippet: .. EPCs were isolated from samples using Ficoll-Paque and five million cells were seeded on 6-well fibronectin-coated dishes (BD biosciences) in CFU-Hill medium (stem cell technologies, cat05900) ( ). ..

Gradient Centrifugation:

Article Title: Aberrant Expression of Intracellular let-7e, miR-146a, and miR-155 Correlates with Severity of Depression in Patients with Major Depressive Disorder and Is Ameliorated after Antidepressant Treatment
Article Snippet: .. Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR) Venous blood (15 mL) samples were drawn between 6:00 a.m. and 10:00 a.m., after subjects fasted for 9 h. PBMCs were isolated from venous blood samples by Ficoll–Paque (GE, #17-5442-02) density gradient centrifugation and labeled with BD IMag anti-human CD14 Magnetic Particles DM (BD Biosciences, #557769) according to the manufacturer’s directions. .. RNA samples were extracted using Quick-RNA MiniPrep kits (Zymo Research R1055) and reverse-transcribed to complementary DNA (cDNA) using TaqMan Micro RNA Transcription Kits (Applied Biosystems 4366596).

Article Title: Pilot trial of interleukin-2 and zoledronic acid to augment γδ T cells as treatment for patients with refractory renal cell carcinoma
Article Snippet: .. PBMC were isolated by density gradient centrifugation over Ficoll-Paque using standard techniques and stained with the following fluorochrome-labeled monoclonal antibodies for respective experiments: V γ 9-FITC, V δ 2-PE, CD3-FITC, αβ TCR-PE, CD4-APC, CD8-PE, V δ 2-FITC, CD27-PE, CD45RA-APC (Beckton Dickinson (BD) Bioscience Pharmingen, San Jose, CA), or isotype controls (IgG1-FITC, IgG1-PE (Sigma, St. Louis, MO), IgG1-APC (BD)). .. After labeling, the cells were washed and fixed with paraformaldehyde (Sigma, St. Louis, MO) and the samples analyzed on a FACSCalibur (Becton–Dickinson Biosciences, Mountain View, CA).

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    Becton Dickinson ficoll paque density gradient centrifugation
    Ficoll Paque Density Gradient Centrifugation, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson ficoll paque tubes
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