Structured Review

Avantor ficoll paque
Ficoll Paque, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ficoll paque/product/Avantor
Average 92 stars, based on 7 article reviews
Price from $9.99 to $1999.99
ficoll paque - by Bioz Stars, 2020-07
92/100 stars

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Cell Isolation:

Article Title: Soluble PD-L1 generated by endogenous retroelement exaptation is a receptor antagonist
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were freshly isolated by density gradient centrifugation in Ficoll-Paque (VWR) and CD8+ T cells isolated by negative selection using a CD8+ T Cell Isolation Kit (Miltenyi Biotec). ..

Selection:

Article Title: Soluble PD-L1 generated by endogenous retroelement exaptation is a receptor antagonist
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were freshly isolated by density gradient centrifugation in Ficoll-Paque (VWR) and CD8+ T cells isolated by negative selection using a CD8+ T Cell Isolation Kit (Miltenyi Biotec). ..

Isolation:

Article Title: Co‐inhibitory profile and cytotoxicity of CD57+PD‐1– T cells in end‐stage renal disease patients
Article Snippet: .. Prior to transplantation PBMCs were isolated from heparinized blood by density gradient centrifugation using Ficoll‐Paque (VWR, Amsterdam, the Netherlands), then frozen in supplemented RPMI‐1640 + glutamax (Fisher Scientific, Landsmeer, the Netherlands) with 10% fetal bovine serum (FBS) (Lonza, Verviers, Belgium) and 10% dimethyl sulphoxide (DMSO) (VWR) and stored at −140°C. .. To establish cytotoxic function and co‐inhibitory molecule expression, T cells were sorted by the FACS method.

Article Title: Matrix-metalloproteinase-9 is cleaved and activated by Cathepsin K
Article Snippet: .. PMBC were isolated from buffy coats by Ficoll-Paque according to manufactures protocol (VWR, Germany), and CD14+ monocytes were isolated using CD14 magnetically labeled micro beads as per the manufactures protocol (Miltenyi Biotec, Germany). .. Monocytes were seeded at a concentration of 1.5 × 106 cell/cm2 and cultured for 4 days in alpha-MEM (Invitrogen, Germany) basic medium (HEPES, sodium pyruvate, penicillin–streptomycin-glutamine, 10% FCS) supplemented with 25 ng/mL rhM-CSF (RnD Systems, Germany) after which the media was supplemented with 50 ng/mL rhRANK-L (RnD Systems, Germany).

Article Title: Natural Killer Cells Adapt to Cytomegalovirus Along a Functionally Static Phenotypic Spectrum in Human Immunodeficiency Virus Infection
Article Snippet: .. Whole blood was collected with informed consent from healthy donors and peripheral blood mononuclear cells (PBMC) isolated by Ficoll-Paque (VWR, Mississauga, ON, Canada) density gradient centrifugation were suspended in lymphocyte medium consisting of RPMI-1640 supplemented with 10% fetal calf serum (FCS), 200 IU/mL penicillin/streptomycin (P/S), 1% 1 M HEPES, 1% L-glutamine (all from Invitrogen, Carlsbad, CA, USA) and 2.0 × 10−5 M 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA). .. Individuals infected with HIV recruited through the Newfoundland and Labrador Provincial HIV Clinic provided informed consent for whole blood collection, immunological studies, and researcher access to medical laboratory records.

Article Title: Concentric and Eccentric Endurance Exercise Reverse Hallmarks of T-Cell Senescence in Pre-diabetic Subjects
Article Snippet: .. Analysis of T-Cell Subpopulations PBMCs (peripheral blood mononuclear cells) were isolated by density gradient centrifugation using Ficoll paque (VWR, Austria) and frozen (-196°C) for analysis at a later time point. .. Analysis of T-cell subpopulations was performed by flow cytometry (FACSCANTO II, BD, United States) using specific labeled antibodies against CD3 (APCCy7), CD4 (APC), CD8 (BV421 or PECy7), CD45RO (PE), CCR7 (FITC), CD16 (FITC), CD19 (PE), and CD25 (PE) all from BD/Europe, Biozym/Germany or eBioscience/Austria and the viability dye 7-AAD (Sigma/Germany).

Article Title: Soluble PD-L1 generated by endogenous retroelement exaptation is a receptor antagonist
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were freshly isolated by density gradient centrifugation in Ficoll-Paque (VWR) and CD8+ T cells isolated by negative selection using a CD8+ T Cell Isolation Kit (Miltenyi Biotec). ..

Labeling:

Article Title: Matrix-metalloproteinase-9 is cleaved and activated by Cathepsin K
Article Snippet: .. PMBC were isolated from buffy coats by Ficoll-Paque according to manufactures protocol (VWR, Germany), and CD14+ monocytes were isolated using CD14 magnetically labeled micro beads as per the manufactures protocol (Miltenyi Biotec, Germany). .. Monocytes were seeded at a concentration of 1.5 × 106 cell/cm2 and cultured for 4 days in alpha-MEM (Invitrogen, Germany) basic medium (HEPES, sodium pyruvate, penicillin–streptomycin-glutamine, 10% FCS) supplemented with 25 ng/mL rhM-CSF (RnD Systems, Germany) after which the media was supplemented with 50 ng/mL rhRANK-L (RnD Systems, Germany).

Transplantation Assay:

Article Title: Co‐inhibitory profile and cytotoxicity of CD57+PD‐1– T cells in end‐stage renal disease patients
Article Snippet: .. Prior to transplantation PBMCs were isolated from heparinized blood by density gradient centrifugation using Ficoll‐Paque (VWR, Amsterdam, the Netherlands), then frozen in supplemented RPMI‐1640 + glutamax (Fisher Scientific, Landsmeer, the Netherlands) with 10% fetal bovine serum (FBS) (Lonza, Verviers, Belgium) and 10% dimethyl sulphoxide (DMSO) (VWR) and stored at −140°C. .. To establish cytotoxic function and co‐inhibitory molecule expression, T cells were sorted by the FACS method.

Gradient Centrifugation:

Article Title: Co‐inhibitory profile and cytotoxicity of CD57+PD‐1– T cells in end‐stage renal disease patients
Article Snippet: .. Prior to transplantation PBMCs were isolated from heparinized blood by density gradient centrifugation using Ficoll‐Paque (VWR, Amsterdam, the Netherlands), then frozen in supplemented RPMI‐1640 + glutamax (Fisher Scientific, Landsmeer, the Netherlands) with 10% fetal bovine serum (FBS) (Lonza, Verviers, Belgium) and 10% dimethyl sulphoxide (DMSO) (VWR) and stored at −140°C. .. To establish cytotoxic function and co‐inhibitory molecule expression, T cells were sorted by the FACS method.

Article Title: Natural Killer Cells Adapt to Cytomegalovirus Along a Functionally Static Phenotypic Spectrum in Human Immunodeficiency Virus Infection
Article Snippet: .. Whole blood was collected with informed consent from healthy donors and peripheral blood mononuclear cells (PBMC) isolated by Ficoll-Paque (VWR, Mississauga, ON, Canada) density gradient centrifugation were suspended in lymphocyte medium consisting of RPMI-1640 supplemented with 10% fetal calf serum (FCS), 200 IU/mL penicillin/streptomycin (P/S), 1% 1 M HEPES, 1% L-glutamine (all from Invitrogen, Carlsbad, CA, USA) and 2.0 × 10−5 M 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA). .. Individuals infected with HIV recruited through the Newfoundland and Labrador Provincial HIV Clinic provided informed consent for whole blood collection, immunological studies, and researcher access to medical laboratory records.

Article Title: Concentric and Eccentric Endurance Exercise Reverse Hallmarks of T-Cell Senescence in Pre-diabetic Subjects
Article Snippet: .. Analysis of T-Cell Subpopulations PBMCs (peripheral blood mononuclear cells) were isolated by density gradient centrifugation using Ficoll paque (VWR, Austria) and frozen (-196°C) for analysis at a later time point. .. Analysis of T-cell subpopulations was performed by flow cytometry (FACSCANTO II, BD, United States) using specific labeled antibodies against CD3 (APCCy7), CD4 (APC), CD8 (BV421 or PECy7), CD45RO (PE), CCR7 (FITC), CD16 (FITC), CD19 (PE), and CD25 (PE) all from BD/Europe, Biozym/Germany or eBioscience/Austria and the viability dye 7-AAD (Sigma/Germany).

Article Title: Soluble PD-L1 generated by endogenous retroelement exaptation is a receptor antagonist
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were freshly isolated by density gradient centrifugation in Ficoll-Paque (VWR) and CD8+ T cells isolated by negative selection using a CD8+ T Cell Isolation Kit (Miltenyi Biotec). ..

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    Avantor triton x 100
    Triton X 100, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triton x 100/product/Avantor
    Average 93 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    triton x 100 - by Bioz Stars, 2020-07
    93/100 stars
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