fibulin 5  (Wuhan Feiyue Biotechnology Co Ltd)

Journal: Food Science & Nutrition

Article Title: Anti‐skin aging effect of sea buckthorn proanthocyanidins in D‐galactose‐induced aging mice

doi: 10.1002/fsn3.3823

Figure Lengend Snippet: The effect of SBP on Col I and tropoelastin synthesis pathways. (a) Associated protein bands. (b, c) Expression of TGF‐β1, Smad3, Col I, Elastin, LOX, and Fibulin 5 in skin tissues of different experimental groups. Protein blots are representative of at least three independent experiments. (d) Scanning electron microscopic observation of fibrous tissue in the dermis of mice skin tissue. a‐f Mean values within the same row not sharing a common superscript letter are significantly different ( p < .05) by Duncan's multiple range test.

Article Snippet: Primary antibodies: anti‐Smad 3 was purchased from Proma (Hunan, China, cat. no. 30072); anti‐TGF‐β1 was purchased from ABclonal (Wuhan, China, cat. no. A2124); and anti‐type I collagen (cat. no. ab260043), anti‐tropoelastin (cat. no. Ab217356), anti‐LOX (cat. no. ab174316), and anti‐Fibulin 5 (cat. no. ab109428) were purchased from Abcam (Cambridge, MA, USA). β‐Actin was purchased from Protein Group, Inc. (Wuhan, China, cat. no. 66009‐1‐Ig).

Techniques: Expressing