Review





Similar Products

human lung fibroblast cell line  (ATCC)
99
ATCC human lung fibroblast cell line
Direct effect of dextrose exposure on <t>fibroblast</t> metabolic activity immediately following treatment. XTT assay, displaying optical density (OD) readings of fibroblasts treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, run in duplicate, n = 5, error bars represent SEM, * P <0.05.
Human Lung Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human lung fibroblast cell line/product/ATCC
Average 99 stars, based on 1 article reviews
human lung fibroblast cell line - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
human fibroblast cell line mrc 5  (ATCC)
99
ATCC human fibroblast cell line mrc 5
Direct effect of dextrose exposure on <t>fibroblast</t> metabolic activity immediately following treatment. XTT assay, displaying optical density (OD) readings of fibroblasts treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, run in duplicate, n = 5, error bars represent SEM, * P <0.05.
Human Fibroblast Cell Line Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human fibroblast cell line mrc 5/product/ATCC
Average 99 stars, based on 1 article reviews
human fibroblast cell line mrc 5 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
human lung fibroblast mrc5 cells  (ATCC)
99
ATCC human lung fibroblast mrc5 cells
Direct effect of dextrose exposure on <t>fibroblast</t> metabolic activity immediately following treatment. XTT assay, displaying optical density (OD) readings of fibroblasts treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, run in duplicate, n = 5, error bars represent SEM, * P <0.05.
Human Lung Fibroblast Mrc5 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human lung fibroblast mrc5 cells/product/ATCC
Average 99 stars, based on 1 article reviews
human lung fibroblast mrc5 cells - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
normal human lung fibroblast cell line mrc  (ATCC)
99
ATCC normal human lung fibroblast cell line mrc
Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase <t>on</t> <t>MRC-5</t> cells. The results denote the average of the data received from three experiments and the error bars show standard deviation
Normal Human Lung Fibroblast Cell Line Mrc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/normal human lung fibroblast cell line mrc/product/ATCC
Average 99 stars, based on 1 article reviews
normal human lung fibroblast cell line mrc - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
human fibroblast cells mrc 5  (ATCC)
99
ATCC human fibroblast cells mrc 5
Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase <t>on</t> <t>MRC-5</t> cells. The results denote the average of the data received from three experiments and the error bars show standard deviation
Human Fibroblast Cells Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human fibroblast cells mrc 5/product/ATCC
Average 99 stars, based on 1 article reviews
human fibroblast cells mrc 5 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
lung fibroblast cell line mrc 5  (ATCC)
99
ATCC lung fibroblast cell line mrc 5
Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase <t>on</t> <t>MRC-5</t> cells. The results denote the average of the data received from three experiments and the error bars show standard deviation
Lung Fibroblast Cell Line Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lung fibroblast cell line mrc 5/product/ATCC
Average 99 stars, based on 1 article reviews
lung fibroblast cell line mrc 5 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier

Image Search Results


Direct effect of dextrose exposure on fibroblast metabolic activity immediately following treatment. XTT assay, displaying optical density (OD) readings of fibroblasts treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, run in duplicate, n = 5, error bars represent SEM, * P <0.05.

Journal: Cartilage

Article Title: In Vitro Model Exploring the Mechanisms of Dextrose Prolotherapy: Fibroblasts Exposed to Clinical Concentrations of Dextrose Exhibit Significant Rebound Effects 48 Hours After Exposure

doi: 10.1177/19476035251408601

Figure Lengend Snippet: Direct effect of dextrose exposure on fibroblast metabolic activity immediately following treatment. XTT assay, displaying optical density (OD) readings of fibroblasts treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, run in duplicate, n = 5, error bars represent SEM, * P <0.05.

Article Snippet: The MRC-5 cells, a human lung fibroblast cell line (ATCC; cat# CCL-171), were cultured in complete medium made from Eagle’s Minimum Essential Medium (ATCC; cat# 3002003) supplemented with 10% fetal bovine serum (Atlanta Biologicals; cat# S11150 ), 1% penicillin/streptomycin (ATCC; cat# 30-2300), and 2 mM L-glutamine (ATCC; cat# 30-2214) according to the protocol for subculture recommended by ATCC.

Techniques: Activity Assay, XTT Assay, Control

Direct effect of dextrose exposure on fibroblast metabolic activity 48 hours after treatment. After fibroblasts were treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, the supernatant fluid was replaced with fresh media and the cells were maintained at 37°C, 5% CO 2 for 48 hours prior to running the XTT assay, displaying optical density (OD) readings, run in triplicate, n = 5, error bars represent SEM, * P < 0.05.

Journal: Cartilage

Article Title: In Vitro Model Exploring the Mechanisms of Dextrose Prolotherapy: Fibroblasts Exposed to Clinical Concentrations of Dextrose Exhibit Significant Rebound Effects 48 Hours After Exposure

doi: 10.1177/19476035251408601

Figure Lengend Snippet: Direct effect of dextrose exposure on fibroblast metabolic activity 48 hours after treatment. After fibroblasts were treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, the supernatant fluid was replaced with fresh media and the cells were maintained at 37°C, 5% CO 2 for 48 hours prior to running the XTT assay, displaying optical density (OD) readings, run in triplicate, n = 5, error bars represent SEM, * P < 0.05.

Article Snippet: The MRC-5 cells, a human lung fibroblast cell line (ATCC; cat# CCL-171), were cultured in complete medium made from Eagle’s Minimum Essential Medium (ATCC; cat# 3002003) supplemented with 10% fetal bovine serum (Atlanta Biologicals; cat# S11150 ), 1% penicillin/streptomycin (ATCC; cat# 30-2300), and 2 mM L-glutamine (ATCC; cat# 30-2214) according to the protocol for subculture recommended by ATCC.

Techniques: Activity Assay, Control, XTT Assay

Percent change in fibroblast metabolic activity comparing immediately following dextrose treatment to 48 hours following treatment. MRC-5 fibroblasts were exposed to concentrations of 5%, 10%, 15%, 20%, or 25% dextrose for 15, 30, 60, or 120 min. Absorbance was measured by XTT assay immediately following these treatment conditions and then also 48 hours following each of these treatment conditions. Average absorbance values using the XTT assay were calculated for each type of treatment: (1) immediately following direct treatment with dextrose and (2) 48 hours following direct treatment. The percent change was calculated as a ratio of the 48-hour average absorbance value minus the immediate average absorbance value divided by the average absorbance value immediately following treatment for each matched dextrose concentration and exposure time. These values were normalized to the corresponding media control for each duration of dextrose treatment (15, 30, 60, or 120 min). Averaged values represent 5 independent experiments.

Journal: Cartilage

Article Title: In Vitro Model Exploring the Mechanisms of Dextrose Prolotherapy: Fibroblasts Exposed to Clinical Concentrations of Dextrose Exhibit Significant Rebound Effects 48 Hours After Exposure

doi: 10.1177/19476035251408601

Figure Lengend Snippet: Percent change in fibroblast metabolic activity comparing immediately following dextrose treatment to 48 hours following treatment. MRC-5 fibroblasts were exposed to concentrations of 5%, 10%, 15%, 20%, or 25% dextrose for 15, 30, 60, or 120 min. Absorbance was measured by XTT assay immediately following these treatment conditions and then also 48 hours following each of these treatment conditions. Average absorbance values using the XTT assay were calculated for each type of treatment: (1) immediately following direct treatment with dextrose and (2) 48 hours following direct treatment. The percent change was calculated as a ratio of the 48-hour average absorbance value minus the immediate average absorbance value divided by the average absorbance value immediately following treatment for each matched dextrose concentration and exposure time. These values were normalized to the corresponding media control for each duration of dextrose treatment (15, 30, 60, or 120 min). Averaged values represent 5 independent experiments.

Article Snippet: The MRC-5 cells, a human lung fibroblast cell line (ATCC; cat# CCL-171), were cultured in complete medium made from Eagle’s Minimum Essential Medium (ATCC; cat# 3002003) supplemented with 10% fetal bovine serum (Atlanta Biologicals; cat# S11150 ), 1% penicillin/streptomycin (ATCC; cat# 30-2300), and 2 mM L-glutamine (ATCC; cat# 30-2214) according to the protocol for subculture recommended by ATCC.

Techniques: Activity Assay, XTT Assay, Concentration Assay, Control

Indirect effect of dextrose exposure on the metabolic activity of nascent fibroblasts 48 hours after treatment. After fibroblasts were treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, the supernatant fluid was replaced with fresh media and the cells were maintained at 37°C, 5% CO 2 for 8 hours prior to collecting the supernatant fluid. Nascent fibroblasts (not exposed to dextrose) were then incubated with this supernatant fluid from dextrose-treated cells for 48 hours. XTT assay, displaying optical density (OD) readings of nascent fibroblasts 48 hours after being exposed to supernatant fluid taken from fibroblasts directly treated with dextrose, run in triplicate, n = 5, error bars represent SEM. * P < 0.05.

Journal: Cartilage

Article Title: In Vitro Model Exploring the Mechanisms of Dextrose Prolotherapy: Fibroblasts Exposed to Clinical Concentrations of Dextrose Exhibit Significant Rebound Effects 48 Hours After Exposure

doi: 10.1177/19476035251408601

Figure Lengend Snippet: Indirect effect of dextrose exposure on the metabolic activity of nascent fibroblasts 48 hours after treatment. After fibroblasts were treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, the supernatant fluid was replaced with fresh media and the cells were maintained at 37°C, 5% CO 2 for 8 hours prior to collecting the supernatant fluid. Nascent fibroblasts (not exposed to dextrose) were then incubated with this supernatant fluid from dextrose-treated cells for 48 hours. XTT assay, displaying optical density (OD) readings of nascent fibroblasts 48 hours after being exposed to supernatant fluid taken from fibroblasts directly treated with dextrose, run in triplicate, n = 5, error bars represent SEM. * P < 0.05.

Article Snippet: The MRC-5 cells, a human lung fibroblast cell line (ATCC; cat# CCL-171), were cultured in complete medium made from Eagle’s Minimum Essential Medium (ATCC; cat# 3002003) supplemented with 10% fetal bovine serum (Atlanta Biologicals; cat# S11150 ), 1% penicillin/streptomycin (ATCC; cat# 30-2300), and 2 mM L-glutamine (ATCC; cat# 30-2214) according to the protocol for subculture recommended by ATCC.

Techniques: Activity Assay, Control, Incubation, XTT Assay

Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase on MRC-5 cells. The results denote the average of the data received from three experiments and the error bars show standard deviation

Journal: Microbial Cell Factories

Article Title: Recombinant L-asparaginase from Stenotrophomonas maltophilia : a promising low-immunogenic anticancer agent

doi: 10.1186/s12934-025-02856-0

Figure Lengend Snippet: Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase on MRC-5 cells. The results denote the average of the data received from three experiments and the error bars show standard deviation

Article Snippet: Human leukemia cancer cell line K-562, human hepatocellular carcinoma cell line HepG-2, and normal human lung fibroblast cell line MRC-5 were purchased from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Activity Assay, Recombinant, Produced, Standard Deviation