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Biological Industries Inc fetal bovine serum fbs
Validation of exosome isolation Exosomes were isolated from Jurkat, <t>K562,</t> MCF7 and HCT116 cells growth medium depleted from <t>FBS</t> exosomes as described in the Methods section following 72 hours of growth. A . Exosomal markers from all cells lines analyzed by Western blotting; B. Capture imaging of Jurkat exosomes obtained by using the NanoSight device; C. amount of these exosomes obtained specified by the NanoSight device.
Fetal Bovine Serum Fbs, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 94/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fetal bovine serum fbs/product/Biological Industries Inc
Average 94 stars, based on 313 article reviews
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fetal bovine serum fbs - by Bioz Stars, 2020-07
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Images

1) Product Images from "Tumor cells derived exosomes contain hTERT mRNA and transform nonmalignant fibroblasts into telomerase positive cells"

Article Title: Tumor cells derived exosomes contain hTERT mRNA and transform nonmalignant fibroblasts into telomerase positive cells

Journal: Oncotarget

doi: 10.18632/oncotarget.10384

Validation of exosome isolation Exosomes were isolated from Jurkat, K562, MCF7 and HCT116 cells growth medium depleted from FBS exosomes as described in the Methods section following 72 hours of growth. A . Exosomal markers from all cells lines analyzed by Western blotting; B. Capture imaging of Jurkat exosomes obtained by using the NanoSight device; C. amount of these exosomes obtained specified by the NanoSight device.
Figure Legend Snippet: Validation of exosome isolation Exosomes were isolated from Jurkat, K562, MCF7 and HCT116 cells growth medium depleted from FBS exosomes as described in the Methods section following 72 hours of growth. A . Exosomal markers from all cells lines analyzed by Western blotting; B. Capture imaging of Jurkat exosomes obtained by using the NanoSight device; C. amount of these exosomes obtained specified by the NanoSight device.

Techniques Used: Isolation, Western Blot, Imaging

2) Product Images from "Role of YAP in lung cancer resistance to cisplatin"

Article Title: Role of YAP in lung cancer resistance to cisplatin

Journal: Oncology Letters

doi: 10.3892/ol.2018.9141

Morphologies of A549 adherent cells and A549 spheres. Adherent A549 cells were cultured in DMEM medium supplemented with 10% fetal bovine serum and floating A549 spheres were cultured in serum-free DMEM/F-12 medium. DMEM, Dulbecco's modified Eagle's medium.
Figure Legend Snippet: Morphologies of A549 adherent cells and A549 spheres. Adherent A549 cells were cultured in DMEM medium supplemented with 10% fetal bovine serum and floating A549 spheres were cultured in serum-free DMEM/F-12 medium. DMEM, Dulbecco's modified Eagle's medium.

Techniques Used: Cell Culture, Modification

3) Product Images from "Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity"

Article Title: Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity

Journal: Molecules

doi: 10.3390/molecules24203667

F10 cell viability after 48-h incubation in the presence of different concentrations of FLs in control culture medium supplemented with 10% fetal bovine serum (FBS) ( a ) or 2% NutriStem V9 XF Basal Medium (XF) ( b ). Each value represents mean ± SD ( n = 5). Significant differences vs. no addition (0 μg/mL): * p
Figure Legend Snippet: F10 cell viability after 48-h incubation in the presence of different concentrations of FLs in control culture medium supplemented with 10% fetal bovine serum (FBS) ( a ) or 2% NutriStem V9 XF Basal Medium (XF) ( b ). Each value represents mean ± SD ( n = 5). Significant differences vs. no addition (0 μg/mL): * p

Techniques Used: Incubation

4) Product Images from "Prospective Identification of Glioblastoma Cells Generating Dormant Tumors"

Article Title: Prospective Identification of Glioblastoma Cells Generating Dormant Tumors

Journal: PLoS ONE

doi: 10.1371/journal.pone.0044395

Angiogenic potential comparison between cells of U-87 MG and Clone #1. A. C.M. of U-87 MG cells induced extensive sprouting of endothelial cells (upper panel) from aortic rings resected from mice as compared with C.M. of Clone #1 (lower panel). B. HUVEC migrate towards C.M. of U-87 MG cells (red bar) at a significantly increased rate ( p = 1.6×10 −13 ) compared with that of Clone #1 (black bar). DMEM containing 10% FBS served as positive control; DMEM served as negative control. C. HUVEC’s ability to form capillary-like tube structures on Matrigel® is significantly higher ( p = 0.001) in the presence of C.M. of U-87 MG cells (red bar) compared with that in the presence of C.M. of Clone #1 cells (black bar). D. Contrast-enhanced ultrasound imaging of subcutaneously-inoculated plugs containing C.M. media from U-87 MG cells (red bar, n = 3) showed increased vascularization compared with C.M. from Clone #1 cells (black bar, n = 3) ( p = 0.04). Data represent mean± s.d. from three independent experiments. * p
Figure Legend Snippet: Angiogenic potential comparison between cells of U-87 MG and Clone #1. A. C.M. of U-87 MG cells induced extensive sprouting of endothelial cells (upper panel) from aortic rings resected from mice as compared with C.M. of Clone #1 (lower panel). B. HUVEC migrate towards C.M. of U-87 MG cells (red bar) at a significantly increased rate ( p = 1.6×10 −13 ) compared with that of Clone #1 (black bar). DMEM containing 10% FBS served as positive control; DMEM served as negative control. C. HUVEC’s ability to form capillary-like tube structures on Matrigel® is significantly higher ( p = 0.001) in the presence of C.M. of U-87 MG cells (red bar) compared with that in the presence of C.M. of Clone #1 cells (black bar). D. Contrast-enhanced ultrasound imaging of subcutaneously-inoculated plugs containing C.M. media from U-87 MG cells (red bar, n = 3) showed increased vascularization compared with C.M. from Clone #1 cells (black bar, n = 3) ( p = 0.04). Data represent mean± s.d. from three independent experiments. * p

Techniques Used: Mouse Assay, Positive Control, Negative Control, Imaging

Related Articles

Modification:

Article Title: Role of YAP in lung cancer resistance to cisplatin
Article Snippet: .. Cell culture media Dulbecco's modified Eagle's medium (DMEM), DMEM/F-12 and fetal bovine serum (FBS) were obtained from Biological Industries (Cromwell, CT, USA). .. Trypsin and EDTA were obtained from Solarbio (Beijing, China), B27 was purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA), EGF and bFGF were obtained from Proteintech (Rosemont, IL, USA).

Article Title: Prospective Identification of Glioblastoma Cells Generating Dormant Tumors
Article Snippet: .. Materials Dulbecco’s modified Eagle’s medium (DMEM), Fetal Bovine Serum (FBS), Penicillin, Streptomycin, Nystatin, and L-glutamine were purchased from Biological Industries (Kibbutz Beit Haemek, Israel). .. Matrigel®, growth factor-reduced Matrigel and Bacto™ Agar were from BD Bioscience (Franklin Lake, NJ, USA).

Article Title: Combination Therapy of PEG-HM-3 and Methotrexate Retards Adjuvant-Induced Arthritis
Article Snippet: .. Splenocytes (1.0 × 106 cells·mL−1 ) were subsequently washed and suspended in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Gaithersburg, MD, USA) containing 10% fetal bovine serum (FBS) (Biological Industries, Beit HaEmek, Israel), and were cultured at 37 °C in 96-well plates in 5% CO2 for 24 h. Vehicle or ConA (5 μg·mL−1 ) were used as a negative or positive control. .. Splenocytes were treated with different doses of PEG-HM-3 (1.13–72.0 μM) in the presence or absence of MTX (1, 2, 4 μM) (Hengrui medicine Co Ltd., Lianyungang, China) for 48 h. RAW264.7 cells were maintained in RPMI 1640 culture medium (Gibco, USA) with 10% FBS until the logarithmic growth phase.

Incubation:

Article Title: Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity
Article Snippet: .. Influence of BSA on Cytotoxicity of FLs Cell viability following incubation of FLs with 10% fetal bovine serum (FBS) or with 2% XF (NutriStem V9 XF Basal Medium, Biological Industries Ltd., Kibbutz Beit Haemek, Israel) is shown in . .. For F11 cells cultured in a medium supplemented with 10% FBS, FLs did not exert any cytotoxicity up to a concentration of 100 μg/mL; however, most cells died at a concentration of 1000 μg/mL.

Cell Culture:

Article Title: Role of YAP in lung cancer resistance to cisplatin
Article Snippet: .. Cell culture media Dulbecco's modified Eagle's medium (DMEM), DMEM/F-12 and fetal bovine serum (FBS) were obtained from Biological Industries (Cromwell, CT, USA). .. Trypsin and EDTA were obtained from Solarbio (Beijing, China), B27 was purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA), EGF and bFGF were obtained from Proteintech (Rosemont, IL, USA).

Article Title: Tumor cells derived exosomes contain hTERT mRNA and transform nonmalignant fibroblasts into telomerase positive cells
Article Snippet: .. Experimental system Jurkat and K562 cell lines were cultured in RPMI-1640 supplemented with 20% and 10% fetal bovine serum (FBS), respectively, containing 100 units/ml L-glutamine and 1% penicillin/streptomycin (Biological Industries Beit Haemek, Israel). .. MCF-7, HCT-116 and pHFF (primary human foreskin fibroblasts, kindly provided by Sara Selig, the Technion - Israel Institute of Technology, Israel) cell lines were cultured in DMEM with 10% FBS supplemented with the same supplements as mentioned for Jurkat and K562 cells under standard incubation conditions.

Article Title: Combination Therapy of PEG-HM-3 and Methotrexate Retards Adjuvant-Induced Arthritis
Article Snippet: .. Splenocytes (1.0 × 106 cells·mL−1 ) were subsequently washed and suspended in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Gaithersburg, MD, USA) containing 10% fetal bovine serum (FBS) (Biological Industries, Beit HaEmek, Israel), and were cultured at 37 °C in 96-well plates in 5% CO2 for 24 h. Vehicle or ConA (5 μg·mL−1 ) were used as a negative or positive control. .. Splenocytes were treated with different doses of PEG-HM-3 (1.13–72.0 μM) in the presence or absence of MTX (1, 2, 4 μM) (Hengrui medicine Co Ltd., Lianyungang, China) for 48 h. RAW264.7 cells were maintained in RPMI 1640 culture medium (Gibco, USA) with 10% FBS until the logarithmic growth phase.

Article Title: Mechanisms of lung toxicity induced by biomass burning aerosols
Article Snippet: .. Cell culture and exposure The human lung bronchial cell line BEAS2B (ATCC® CRL-9609™) was grown in DMEM (Gibco, Thermo Fisher Scientific, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 5 μg/ml penicillin/streptomycin (Biological Industries) at 37 °C in a humidified atmosphere consisting of 95% air and 5% CO2 . ..

Chloramphenicol Acetyltransferase Assay:

Article Title: The Ferroptosis Inducer Erastin Promotes Proliferation and Differentiation in Human Peripheral Blood Mononuclear Cells
Article Snippet: .. PBMCs were maintained in 1640 medium (Cat8116123, GIBCO, Invitrogen China Limited, Shanghai, China) with 10% fetal bovine serum (FBS) (Cat#104-001-1A, BI, Biological Industries Israel Beit Haemek LTD., Kibbutz Beit-Haemek, Israel). .. PBMCs were counted and suspended in certain drug concentration mediums for drug treatment.

Positive Control:

Article Title: Combination Therapy of PEG-HM-3 and Methotrexate Retards Adjuvant-Induced Arthritis
Article Snippet: .. Splenocytes (1.0 × 106 cells·mL−1 ) were subsequently washed and suspended in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Gaithersburg, MD, USA) containing 10% fetal bovine serum (FBS) (Biological Industries, Beit HaEmek, Israel), and were cultured at 37 °C in 96-well plates in 5% CO2 for 24 h. Vehicle or ConA (5 μg·mL−1 ) were used as a negative or positive control. .. Splenocytes were treated with different doses of PEG-HM-3 (1.13–72.0 μM) in the presence or absence of MTX (1, 2, 4 μM) (Hengrui medicine Co Ltd., Lianyungang, China) for 48 h. RAW264.7 cells were maintained in RPMI 1640 culture medium (Gibco, USA) with 10% FBS until the logarithmic growth phase.

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    Biological Industries Inc fbs
    Effect of OA-GL12 on the repair rate of HaCaT cells scratches HaCaT cells (2.5 × 10 5 /well) were cultured in 24-well plates for 12–24 h with serum starvation and then made to the mechanical scratch wound by a sterile pipette tip. After washed, cells were cultured for next periods (from 0 to 24 h) in a serum-free basal medium with the continued presence of OA-GL12. The same volume of <t>DMEM</t> (serum free) was added as vehicle and OM-LV20 (50 nM) as positive control. ( A ). OA-GL12 (10 pM) showed prominent wound-healing capacity on HaCaT cells. ( B ). OA-GL12 illustrated time- and concentration- dependent wound-healing capacity on HaCaT cells. Contain cells were added into 96-well plates with basic DMEM (90 μl) without 10% <t>FBS</t> and incubated for 2-4 h. Afterward, OA-GL12 (10 μl) was added to each well and incubated for 24 h at various final concentrations then tested using a CellTiter 96® AQueous One Solution Assay. ( C ). OM-LV20 (50 nM) showed proliferative effect, but OA-GL12 did not show on HaCaT cells from 2 to 10 pM. Data were presented as mean ± S.D., n =9. * P
    Fbs, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 93/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Biological Industries Inc dextran coated charcoal stripped fbs
    AR antagonist inhibited U87 proliferation in vitro and in vivo , but it had no effect on U251 ( A ) U251 and ( B ) U87 MTT assay. Cells cultured in phenol red-free <t>DMEM</t> containing 5% (v/v) charcoal stripped fetal bovine serum supplemented with 0.1% (v/v) DMSO or 10 nM R1881 or 10 μM ARN-509 or 10 μM MDV3100 (final concentration), error bar indicates standard deviation, ** P
    Dextran Coated Charcoal Stripped Fbs, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dextran coated charcoal stripped fbs/product/Biological Industries Inc
    Average 88 stars, based on 5 article reviews
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    91
    Biological Industries Inc mineralo organic particles fbs
    <t>Mineralo-organic</t> particles induce NET release by neutrophils. Mineralo-organic particles were prepared by adding 3 mM of CaCl 2 and NaH 2 PO 4 each in DMEM containing ( A ) 0.1% or ( B ) 3% <t>FBS,</t> prior to incubation and preparation for TEM without staining as described in Methods . Data are representative of three independent experiments. Scale bars: 200 nm. ( C ) Live cell imaging of human neutrophils stained with Hoechst 33342 (blue) and treated at time 0 with mineral particles (labeled with FITC-bovine serum albumin; FITC-BSA; green). NET-associated DNA was stained with Sytox (red). Data are representative of three independent experiments. Scale bars: 10 μm. Neutrophils were incubated for 2 hours in the absence ( D ) or presence of particles in 0.1% FBS ( E ), 0.12 mg/ml bovine serum fetuin-A (BSF) ( F ), or 40 mg/ml BSF ( G ). NET-associated DNA was stained green by Sytox. Data are representative of three independent experiments. Scale bars: 20 μm. ( H ) Percentage of neutrophils forming NETs 2 hours after addition of particles. Data are shown as means ± standard errors of the mean (SEM) and the results of at least three independent experiments. ** p
    Mineralo Organic Particles Fbs, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of OA-GL12 on the repair rate of HaCaT cells scratches HaCaT cells (2.5 × 10 5 /well) were cultured in 24-well plates for 12–24 h with serum starvation and then made to the mechanical scratch wound by a sterile pipette tip. After washed, cells were cultured for next periods (from 0 to 24 h) in a serum-free basal medium with the continued presence of OA-GL12. The same volume of DMEM (serum free) was added as vehicle and OM-LV20 (50 nM) as positive control. ( A ). OA-GL12 (10 pM) showed prominent wound-healing capacity on HaCaT cells. ( B ). OA-GL12 illustrated time- and concentration- dependent wound-healing capacity on HaCaT cells. Contain cells were added into 96-well plates with basic DMEM (90 μl) without 10% FBS and incubated for 2-4 h. Afterward, OA-GL12 (10 μl) was added to each well and incubated for 24 h at various final concentrations then tested using a CellTiter 96® AQueous One Solution Assay. ( C ). OM-LV20 (50 nM) showed proliferative effect, but OA-GL12 did not show on HaCaT cells from 2 to 10 pM. Data were presented as mean ± S.D., n =9. * P

    Journal: Bioscience Reports

    Article Title: A short peptide potentially promotes the healing of skin wound

    doi: 10.1042/BSR20181734

    Figure Lengend Snippet: Effect of OA-GL12 on the repair rate of HaCaT cells scratches HaCaT cells (2.5 × 10 5 /well) were cultured in 24-well plates for 12–24 h with serum starvation and then made to the mechanical scratch wound by a sterile pipette tip. After washed, cells were cultured for next periods (from 0 to 24 h) in a serum-free basal medium with the continued presence of OA-GL12. The same volume of DMEM (serum free) was added as vehicle and OM-LV20 (50 nM) as positive control. ( A ). OA-GL12 (10 pM) showed prominent wound-healing capacity on HaCaT cells. ( B ). OA-GL12 illustrated time- and concentration- dependent wound-healing capacity on HaCaT cells. Contain cells were added into 96-well plates with basic DMEM (90 μl) without 10% FBS and incubated for 2-4 h. Afterward, OA-GL12 (10 μl) was added to each well and incubated for 24 h at various final concentrations then tested using a CellTiter 96® AQueous One Solution Assay. ( C ). OM-LV20 (50 nM) showed proliferative effect, but OA-GL12 did not show on HaCaT cells from 2 to 10 pM. Data were presented as mean ± S.D., n =9. * P

    Article Snippet: Briefly, human keratinocytes (HaCaT) (KCB 200442YJ) and human skin fibroblasts (HSF) (KCB 200537) were provided by Conservation Genetics CAS Kunming Cell Bank of Kunming Institute of Zoology, the Chinese Academy of Sciences, and cultured in Dulbecco’s modified Eagle’s medium (DMEM, BI, Israel) with 10% FBS (BI, Israel) and penicillin (100 units/ml)–streptomycin (100 units/ml) in a humidified atmosphere of 5% CO2 (37°C).

    Techniques: Cell Culture, Transferring, Positive Control, Concentration Assay, Incubation

    Effect of OA-GL12 on the repair rate of HSF cell scratches HSF cells (2.5 × 10 5 /well) were cultured in 24-well plates for 12–24 h and then made to the mechanical scratch wound by a sterile pipette tip. After washing, cells were cultured for next periods in a serum-free basal medium (500 μl) with the continued presence of OA-GL12. The same volume of DMEM (serum free) was added as vehicle. ( A ) OA-GL12 (10 pM) illustrated distinct HSF cells scratch-healing activity. ( B ) OA-GL12 illustrated time- and concentration-dependent HSF cells scratch-healing activity. Contain cells were added into 96-well plates with basic DMEM (90 μl) without 10% FBS and incubated for 2–4 h. Afterward, OA-GL12 (10 μl) was added to each well and incubated for 24 h at various final concentrations then tested using a CellTiter 96® AQueous One Solution Assay. ( C , D ). OA-GL12 showed no impact on the proliferation of HSF and HUVEC cells from 2 pM to 10 nM. Data were presented as mean ± S.D., n =9. * P

    Journal: Bioscience Reports

    Article Title: A short peptide potentially promotes the healing of skin wound

    doi: 10.1042/BSR20181734

    Figure Lengend Snippet: Effect of OA-GL12 on the repair rate of HSF cell scratches HSF cells (2.5 × 10 5 /well) were cultured in 24-well plates for 12–24 h and then made to the mechanical scratch wound by a sterile pipette tip. After washing, cells were cultured for next periods in a serum-free basal medium (500 μl) with the continued presence of OA-GL12. The same volume of DMEM (serum free) was added as vehicle. ( A ) OA-GL12 (10 pM) illustrated distinct HSF cells scratch-healing activity. ( B ) OA-GL12 illustrated time- and concentration-dependent HSF cells scratch-healing activity. Contain cells were added into 96-well plates with basic DMEM (90 μl) without 10% FBS and incubated for 2–4 h. Afterward, OA-GL12 (10 μl) was added to each well and incubated for 24 h at various final concentrations then tested using a CellTiter 96® AQueous One Solution Assay. ( C , D ). OA-GL12 showed no impact on the proliferation of HSF and HUVEC cells from 2 pM to 10 nM. Data were presented as mean ± S.D., n =9. * P

    Article Snippet: Briefly, human keratinocytes (HaCaT) (KCB 200442YJ) and human skin fibroblasts (HSF) (KCB 200537) were provided by Conservation Genetics CAS Kunming Cell Bank of Kunming Institute of Zoology, the Chinese Academy of Sciences, and cultured in Dulbecco’s modified Eagle’s medium (DMEM, BI, Israel) with 10% FBS (BI, Israel) and penicillin (100 units/ml)–streptomycin (100 units/ml) in a humidified atmosphere of 5% CO2 (37°C).

    Techniques: Cell Culture, Transferring, Activity Assay, Concentration Assay, Incubation

    Photograph of an E12.5 embryo following culture for 28 h in DMEM/F12 and 20% FBS. Notice the head, divided brain vesicles, right forelimb bud and somites marked by arrows. Also note subcutaneous haemorrhage in the head region.

    Journal: Journal of Anatomy

    Article Title: A simple method of culture of 11.5-day-old rat embryos in DMEM/F12 and 20% fetal bovine serum

    doi: 10.1046/j.1469-7580.2003.00225.x

    Figure Lengend Snippet: Photograph of an E12.5 embryo following culture for 28 h in DMEM/F12 and 20% FBS. Notice the head, divided brain vesicles, right forelimb bud and somites marked by arrows. Also note subcutaneous haemorrhage in the head region.

    Article Snippet: The embryos from each dam were randomly assigned to two groups: 69 embryos were cultured on 90% heat-inactivated rat serum with 10% double distilled water and additional 1 mg mL−1 glucose (total of 2 mg mL−1 ); 85 embryos cultured on DMEM/F12 (50%/50%) supplemented with 20% FBS (all purchased from Biological Industries, Beth Haemek, Israel).

    Techniques:

    Photograph of an E12.5 embryo following culture for 28 h in DMEM/F12 and 20% FBS. Notice the head, divided brain vesicles, two branchial arches, forelimb bud, cardiac prominence and the attached open yolk sac. The arrowhead points to the optic vesicle.

    Journal: Journal of Anatomy

    Article Title: A simple method of culture of 11.5-day-old rat embryos in DMEM/F12 and 20% fetal bovine serum

    doi: 10.1046/j.1469-7580.2003.00225.x

    Figure Lengend Snippet: Photograph of an E12.5 embryo following culture for 28 h in DMEM/F12 and 20% FBS. Notice the head, divided brain vesicles, two branchial arches, forelimb bud, cardiac prominence and the attached open yolk sac. The arrowhead points to the optic vesicle.

    Article Snippet: The embryos from each dam were randomly assigned to two groups: 69 embryos were cultured on 90% heat-inactivated rat serum with 10% double distilled water and additional 1 mg mL−1 glucose (total of 2 mg mL−1 ); 85 embryos cultured on DMEM/F12 (50%/50%) supplemented with 20% FBS (all purchased from Biological Industries, Beth Haemek, Israel).

    Techniques:

    AR antagonist inhibited U87 proliferation in vitro and in vivo , but it had no effect on U251 ( A ) U251 and ( B ) U87 MTT assay. Cells cultured in phenol red-free DMEM containing 5% (v/v) charcoal stripped fetal bovine serum supplemented with 0.1% (v/v) DMSO or 10 nM R1881 or 10 μM ARN-509 or 10 μM MDV3100 (final concentration), error bar indicates standard deviation, ** P

    Journal: Oncotarget

    Article Title: Regulation of p53wt glioma cell proliferation by androgen receptor-mediated inhibition of small VCP/p97-interacting protein expression

    doi: 10.18632/oncotarget.15509

    Figure Lengend Snippet: AR antagonist inhibited U87 proliferation in vitro and in vivo , but it had no effect on U251 ( A ) U251 and ( B ) U87 MTT assay. Cells cultured in phenol red-free DMEM containing 5% (v/v) charcoal stripped fetal bovine serum supplemented with 0.1% (v/v) DMSO or 10 nM R1881 or 10 μM ARN-509 or 10 μM MDV3100 (final concentration), error bar indicates standard deviation, ** P

    Article Snippet: For treatment with R1881 or ARN-509 and MDV3100 (Enzalutamide, brand name Xtandi) both obtained from APExBio, TX, USA, cells were grown in phenol red-free DMEM (Gibco) medium supplemented with 10% (or indicated concentration, v/v) dextran-coated charcoal stripped FBS (Biological Industries, Israel) for 72 h prior to treatment.

    Techniques: In Vitro, In Vivo, MTT Assay, Cell Culture, Concentration Assay, Standard Deviation

    Mineralo-organic particles induce NET release by neutrophils. Mineralo-organic particles were prepared by adding 3 mM of CaCl 2 and NaH 2 PO 4 each in DMEM containing ( A ) 0.1% or ( B ) 3% FBS, prior to incubation and preparation for TEM without staining as described in Methods . Data are representative of three independent experiments. Scale bars: 200 nm. ( C ) Live cell imaging of human neutrophils stained with Hoechst 33342 (blue) and treated at time 0 with mineral particles (labeled with FITC-bovine serum albumin; FITC-BSA; green). NET-associated DNA was stained with Sytox (red). Data are representative of three independent experiments. Scale bars: 10 μm. Neutrophils were incubated for 2 hours in the absence ( D ) or presence of particles in 0.1% FBS ( E ), 0.12 mg/ml bovine serum fetuin-A (BSF) ( F ), or 40 mg/ml BSF ( G ). NET-associated DNA was stained green by Sytox. Data are representative of three independent experiments. Scale bars: 20 μm. ( H ) Percentage of neutrophils forming NETs 2 hours after addition of particles. Data are shown as means ± standard errors of the mean (SEM) and the results of at least three independent experiments. ** p

    Journal: Scientific Reports

    Article Title: Mineral particles stimulate innate immunity through neutrophil extracellular traps containing HMGB1

    doi: 10.1038/s41598-017-16778-4

    Figure Lengend Snippet: Mineralo-organic particles induce NET release by neutrophils. Mineralo-organic particles were prepared by adding 3 mM of CaCl 2 and NaH 2 PO 4 each in DMEM containing ( A ) 0.1% or ( B ) 3% FBS, prior to incubation and preparation for TEM without staining as described in Methods . Data are representative of three independent experiments. Scale bars: 200 nm. ( C ) Live cell imaging of human neutrophils stained with Hoechst 33342 (blue) and treated at time 0 with mineral particles (labeled with FITC-bovine serum albumin; FITC-BSA; green). NET-associated DNA was stained with Sytox (red). Data are representative of three independent experiments. Scale bars: 10 μm. Neutrophils were incubated for 2 hours in the absence ( D ) or presence of particles in 0.1% FBS ( E ), 0.12 mg/ml bovine serum fetuin-A (BSF) ( F ), or 40 mg/ml BSF ( G ). NET-associated DNA was stained green by Sytox. Data are representative of three independent experiments. Scale bars: 20 μm. ( H ) Percentage of neutrophils forming NETs 2 hours after addition of particles. Data are shown as means ± standard errors of the mean (SEM) and the results of at least three independent experiments. ** p

    Article Snippet: Preparation of mineralo-organic particles FBS (Biological Industries) for particle preparation was heat-inactivated and then filtered through 0.2-μm disc filters.

    Techniques: Incubation, Transmission Electron Microscopy, Staining, Live Cell Imaging, Labeling