fetal bovine serum fbs  (Biological Industries Inc)

Journal: Bioscience Reports

Article Title: A short peptide potentially promotes the healing of skin wound

doi: 10.1042/BSR20181734

Figure Lengend Snippet: Effect of OA-GL12 on the repair rate of HaCaT cells scratches HaCaT cells (2.5 × 10 5 /well) were cultured in 24-well plates for 12–24 h with serum starvation and then made to the mechanical scratch wound by a sterile pipette tip. After washed, cells were cultured for next periods (from 0 to 24 h) in a serum-free basal medium with the continued presence of OA-GL12. The same volume of DMEM (serum free) was added as vehicle and OM-LV20 (50 nM) as positive control. ( A ). OA-GL12 (10 pM) showed prominent wound-healing capacity on HaCaT cells. ( B ). OA-GL12 illustrated time- and concentration- dependent wound-healing capacity on HaCaT cells. Contain cells were added into 96-well plates with basic DMEM (90 μl) without 10% FBS and incubated for 2-4 h. Afterward, OA-GL12 (10 μl) was added to each well and incubated for 24 h at various final concentrations then tested using a CellTiter 96® AQueous One Solution Assay. ( C ). OM-LV20 (50 nM) showed proliferative effect, but OA-GL12 did not show on HaCaT cells from 2 to 10 pM. Data were presented as mean ± S.D., n =9. * P

Article Snippet: Briefly, human keratinocytes (HaCaT) (KCB 200442YJ) and human skin fibroblasts (HSF) (KCB 200537) were provided by Conservation Genetics CAS Kunming Cell Bank of Kunming Institute of Zoology, the Chinese Academy of Sciences, and cultured in Dulbecco’s modified Eagle’s medium (DMEM, BI, Israel) with 10% FBS (BI, Israel) and penicillin (100 units/ml)–streptomycin (100 units/ml) in a humidified atmosphere of 5% CO2 (37°C).

Techniques: Cell Culture, Transferring, Positive Control, Concentration Assay, Incubation

Journal: Bioscience Reports

Article Title: A short peptide potentially promotes the healing of skin wound

doi: 10.1042/BSR20181734

Figure Lengend Snippet: Effect of OA-GL12 on the repair rate of HSF cell scratches HSF cells (2.5 × 10 5 /well) were cultured in 24-well plates for 12–24 h and then made to the mechanical scratch wound by a sterile pipette tip. After washing, cells were cultured for next periods in a serum-free basal medium (500 μl) with the continued presence of OA-GL12. The same volume of DMEM (serum free) was added as vehicle. ( A ) OA-GL12 (10 pM) illustrated distinct HSF cells scratch-healing activity. ( B ) OA-GL12 illustrated time- and concentration-dependent HSF cells scratch-healing activity. Contain cells were added into 96-well plates with basic DMEM (90 μl) without 10% FBS and incubated for 2–4 h. Afterward, OA-GL12 (10 μl) was added to each well and incubated for 24 h at various final concentrations then tested using a CellTiter 96® AQueous One Solution Assay. ( C , D ). OA-GL12 showed no impact on the proliferation of HSF and HUVEC cells from 2 pM to 10 nM. Data were presented as mean ± S.D., n =9. * P

Article Snippet: Briefly, human keratinocytes (HaCaT) (KCB 200442YJ) and human skin fibroblasts (HSF) (KCB 200537) were provided by Conservation Genetics CAS Kunming Cell Bank of Kunming Institute of Zoology, the Chinese Academy of Sciences, and cultured in Dulbecco’s modified Eagle’s medium (DMEM, BI, Israel) with 10% FBS (BI, Israel) and penicillin (100 units/ml)–streptomycin (100 units/ml) in a humidified atmosphere of 5% CO2 (37°C).

Techniques: Cell Culture, Transferring, Activity Assay, Concentration Assay, Incubation

Journal: Journal of Anatomy

Article Title: A simple method of culture of 11.5-day-old rat embryos in DMEM/F12 and 20% fetal bovine serum

doi: 10.1046/j.1469-7580.2003.00225.x

Figure Lengend Snippet: Photograph of an E12.5 embryo following culture for 28 h in DMEM/F12 and 20% FBS. Notice the head, divided brain vesicles, right forelimb bud and somites marked by arrows. Also note subcutaneous haemorrhage in the head region.

Article Snippet: The embryos from each dam were randomly assigned to two groups: 69 embryos were cultured on 90% heat-inactivated rat serum with 10% double distilled water and additional 1 mg mL−1 glucose (total of 2 mg mL−1 ); 85 embryos cultured on DMEM/F12 (50%/50%) supplemented with 20% FBS (all purchased from Biological Industries, Beth Haemek, Israel).

Techniques:

Journal: Journal of Anatomy

Article Title: A simple method of culture of 11.5-day-old rat embryos in DMEM/F12 and 20% fetal bovine serum

doi: 10.1046/j.1469-7580.2003.00225.x

Figure Lengend Snippet: Photograph of an E12.5 embryo following culture for 28 h in DMEM/F12 and 20% FBS. Notice the head, divided brain vesicles, two branchial arches, forelimb bud, cardiac prominence and the attached open yolk sac. The arrowhead points to the optic vesicle.

Article Snippet: The embryos from each dam were randomly assigned to two groups: 69 embryos were cultured on 90% heat-inactivated rat serum with 10% double distilled water and additional 1 mg mL−1 glucose (total of 2 mg mL−1 ); 85 embryos cultured on DMEM/F12 (50%/50%) supplemented with 20% FBS (all purchased from Biological Industries, Beth Haemek, Israel).

Techniques:

Journal: Oncotarget

Article Title: Regulation of p53wt glioma cell proliferation by androgen receptor-mediated inhibition of small VCP/p97-interacting protein expression

doi: 10.18632/oncotarget.15509

Figure Lengend Snippet: AR antagonist inhibited U87 proliferation in vitro and in vivo , but it had no effect on U251 ( A ) U251 and ( B ) U87 MTT assay. Cells cultured in phenol red-free DMEM containing 5% (v/v) charcoal stripped fetal bovine serum supplemented with 0.1% (v/v) DMSO or 10 nM R1881 or 10 μM ARN-509 or 10 μM MDV3100 (final concentration), error bar indicates standard deviation, ** P

Article Snippet: For treatment with R1881 or ARN-509 and MDV3100 (Enzalutamide, brand name Xtandi) both obtained from APExBio, TX, USA, cells were grown in phenol red-free DMEM (Gibco) medium supplemented with 10% (or indicated concentration, v/v) dextran-coated charcoal stripped FBS (Biological Industries, Israel) for 72 h prior to treatment.

Techniques: In Vitro, In Vivo, MTT Assay, Cell Culture, Concentration Assay, Standard Deviation

Journal: Scientific Reports

Article Title: Mineral particles stimulate innate immunity through neutrophil extracellular traps containing HMGB1

doi: 10.1038/s41598-017-16778-4

Figure Lengend Snippet: Mineralo-organic particles induce NET release by neutrophils. Mineralo-organic particles were prepared by adding 3 mM of CaCl 2 and NaH 2 PO 4 each in DMEM containing ( A ) 0.1% or ( B ) 3% FBS, prior to incubation and preparation for TEM without staining as described in Methods . Data are representative of three independent experiments. Scale bars: 200 nm. ( C ) Live cell imaging of human neutrophils stained with Hoechst 33342 (blue) and treated at time 0 with mineral particles (labeled with FITC-bovine serum albumin; FITC-BSA; green). NET-associated DNA was stained with Sytox (red). Data are representative of three independent experiments. Scale bars: 10 μm. Neutrophils were incubated for 2 hours in the absence ( D ) or presence of particles in 0.1% FBS ( E ), 0.12 mg/ml bovine serum fetuin-A (BSF) ( F ), or 40 mg/ml BSF ( G ). NET-associated DNA was stained green by Sytox. Data are representative of three independent experiments. Scale bars: 20 μm. ( H ) Percentage of neutrophils forming NETs 2 hours after addition of particles. Data are shown as means ± standard errors of the mean (SEM) and the results of at least three independent experiments. ** p

Article Snippet: Preparation of mineralo-organic particles FBS (Biological Industries) for particle preparation was heat-inactivated and then filtered through 0.2-μm disc filters.

Techniques: Incubation, Transmission Electron Microscopy, Staining, Live Cell Imaging, Labeling