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Biological Industries Inc fetal bovine serum fbs
Fetal Bovine Serum Fbs, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fetal bovine serum fbs/product/Biological Industries Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fetal bovine serum fbs - by Bioz Stars, 2021-03
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Article Title: Knockdown of PAICS inhibits malignant proliferation of human breast cancer cell lines
Article Snippet: Fetal bovine serum (FBS; #04-001-1A) was obtained from Biological Industries (BI; Israel).

Article Title: Cell Hierarchy and Lineage Commitment in the Bovine Mammary Gland
Article Snippet: Dissociation of bovine mammary organoids into single-cell suspension Organoids were washed in HBSS (Biological Industries) containing 5% FBS (HF) and treated for 3 min first with trypsin-EDTA solution (Biological Industries) and then with dispase enzymatic solution (50 caseinolytic units/ml, BD Biosciences, Bedford, MA) that contained DNAse-I (0.125 mg/ml, Worthington).

Article Title: Highly Stable Tetra-Phenolato Titanium(IV) Agent Formulated into Nanoparticles Demonstrates Anti-Tumoral Activity and Selectivity
Article Snippet: Cells (0.6 × 106 ) in medium containing 1% penicillin/streptomycin antibiotics; 1% l -glutamine; 10% fetal bovine serum (FBS); and 88% medium RPMI-1640, all purchased from Biological Industries Inc., were seeded into a 96-well plate and allowed to attach for a day.

Article Title: PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness
Article Snippet: For differentiation assays, the colonospheres of CSCs were plated on plastic plates in medium containing 2% fetal bovine serum (FBS) for 24–120 h.

Article Title: An Estrogen Receptor Dependent Mechanism of Oroxylin A in the Repression of Inflammatory Response
Article Snippet: Charcoal dextran–stripped FBS (CD–FBS) was purchased from Biological Industries (Kibbutz Beit Haeme of Israel).

Article Title: Effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells in vitro
Article Snippet: The osteoblast media (OB) contained H-DMEM (Hyclone, USA), 10% FBS (BI, USA), 100 nM/L dexamethasone (Sigma, USA), 10 mM/L β-glycerophosphate (Sigma, USA), and 0.2 mM/L ascorbate-2-phosphate (Sigma, USA), while the adipocyte media (AD) contained H-DMEM (Hyclone, USA), 10% FBS (BI, USA), 1 μM/L dexamethasone (Sigma, USA), 0.5 mM/L isobutylmethylxanthine (Sigma, USA), 200 μM/L indomethacin (Sigma, USA), and 10 mg/L insulin (Sigma, USA).

Cell Culture:

Article Title: Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate
Article Snippet: .. Cell culture Human ureteral epithelial cell lines (UECs), which were purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China, were cultured in DMEM medium (Hyclone, China), which was supplemented with 10% FBS (FBS, BI, USA) containing 100 U/ml penicillin and 100 µg/ml streptomycin (Logan, USA) in a water-saturated 5% CO2 /95% air atmosphere at 37 °C. ..

Article Title: Effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells in vitro
Article Snippet: Cell culture and differentiation rbMSCs were obtained from the bone marrow of adult male rats. .. The cells were seeded in basal medium containing L-DMEM (Hyclone, USA) and 8% fetal bovine serum (FBS, BI, USA) and cultured at 37 °C with 5% CO2 . ..

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    Biological Industries Inc fbs
    Effect of OA-GL12 on the repair rate of HaCaT cells scratches HaCaT cells (2.5 × 10 5 /well) were cultured in 24-well plates for 12–24 h with serum starvation and then made to the mechanical scratch wound by a sterile pipette tip. After washed, cells were cultured for next periods (from 0 to 24 h) in a serum-free basal medium with the continued presence of OA-GL12. The same volume of <t>DMEM</t> (serum free) was added as vehicle and OM-LV20 (50 nM) as positive control. ( A ). OA-GL12 (10 pM) showed prominent wound-healing capacity on HaCaT cells. ( B ). OA-GL12 illustrated time- and concentration- dependent wound-healing capacity on HaCaT cells. Contain cells were added into 96-well plates with basic DMEM (90 μl) without 10% <t>FBS</t> and incubated for 2-4 h. Afterward, OA-GL12 (10 μl) was added to each well and incubated for 24 h at various final concentrations then tested using a CellTiter 96® AQueous One Solution Assay. ( C ). OM-LV20 (50 nM) showed proliferative effect, but OA-GL12 did not show on HaCaT cells from 2 to 10 pM. Data were presented as mean ± S.D., n =9. * P
    Fbs, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fbs/product/Biological Industries Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fbs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Biological Industries Inc dextran coated charcoal stripped fbs
    AR antagonist inhibited U87 proliferation in vitro and in vivo , but it had no effect on U251 ( A ) U251 and ( B ) U87 MTT assay. Cells cultured in phenol red-free <t>DMEM</t> containing 5% (v/v) charcoal stripped fetal bovine serum supplemented with 0.1% (v/v) DMSO or 10 nM R1881 or 10 μM ARN-509 or 10 μM MDV3100 (final concentration), error bar indicates standard deviation, ** P
    Dextran Coated Charcoal Stripped Fbs, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dextran coated charcoal stripped fbs/product/Biological Industries Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dextran coated charcoal stripped fbs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Biological Industries Inc mineralo organic particles fbs
    <t>Mineralo-organic</t> particles induce NET release by neutrophils. Mineralo-organic particles were prepared by adding 3 mM of CaCl 2 and NaH 2 PO 4 each in DMEM containing ( A ) 0.1% or ( B ) 3% <t>FBS,</t> prior to incubation and preparation for TEM without staining as described in Methods . Data are representative of three independent experiments. Scale bars: 200 nm. ( C ) Live cell imaging of human neutrophils stained with Hoechst 33342 (blue) and treated at time 0 with mineral particles (labeled with FITC-bovine serum albumin; FITC-BSA; green). NET-associated DNA was stained with Sytox (red). Data are representative of three independent experiments. Scale bars: 10 μm. Neutrophils were incubated for 2 hours in the absence ( D ) or presence of particles in 0.1% FBS ( E ), 0.12 mg/ml bovine serum fetuin-A (BSF) ( F ), or 40 mg/ml BSF ( G ). NET-associated DNA was stained green by Sytox. Data are representative of three independent experiments. Scale bars: 20 μm. ( H ) Percentage of neutrophils forming NETs 2 hours after addition of particles. Data are shown as means ± standard errors of the mean (SEM) and the results of at least three independent experiments. ** p
    Mineralo Organic Particles Fbs, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mineralo organic particles fbs/product/Biological Industries Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mineralo organic particles fbs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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    Effect of OA-GL12 on the repair rate of HaCaT cells scratches HaCaT cells (2.5 × 10 5 /well) were cultured in 24-well plates for 12–24 h with serum starvation and then made to the mechanical scratch wound by a sterile pipette tip. After washed, cells were cultured for next periods (from 0 to 24 h) in a serum-free basal medium with the continued presence of OA-GL12. The same volume of DMEM (serum free) was added as vehicle and OM-LV20 (50 nM) as positive control. ( A ). OA-GL12 (10 pM) showed prominent wound-healing capacity on HaCaT cells. ( B ). OA-GL12 illustrated time- and concentration- dependent wound-healing capacity on HaCaT cells. Contain cells were added into 96-well plates with basic DMEM (90 μl) without 10% FBS and incubated for 2-4 h. Afterward, OA-GL12 (10 μl) was added to each well and incubated for 24 h at various final concentrations then tested using a CellTiter 96® AQueous One Solution Assay. ( C ). OM-LV20 (50 nM) showed proliferative effect, but OA-GL12 did not show on HaCaT cells from 2 to 10 pM. Data were presented as mean ± S.D., n =9. * P

    Journal: Bioscience Reports

    Article Title: A short peptide potentially promotes the healing of skin wound

    doi: 10.1042/BSR20181734

    Figure Lengend Snippet: Effect of OA-GL12 on the repair rate of HaCaT cells scratches HaCaT cells (2.5 × 10 5 /well) were cultured in 24-well plates for 12–24 h with serum starvation and then made to the mechanical scratch wound by a sterile pipette tip. After washed, cells were cultured for next periods (from 0 to 24 h) in a serum-free basal medium with the continued presence of OA-GL12. The same volume of DMEM (serum free) was added as vehicle and OM-LV20 (50 nM) as positive control. ( A ). OA-GL12 (10 pM) showed prominent wound-healing capacity on HaCaT cells. ( B ). OA-GL12 illustrated time- and concentration- dependent wound-healing capacity on HaCaT cells. Contain cells were added into 96-well plates with basic DMEM (90 μl) without 10% FBS and incubated for 2-4 h. Afterward, OA-GL12 (10 μl) was added to each well and incubated for 24 h at various final concentrations then tested using a CellTiter 96® AQueous One Solution Assay. ( C ). OM-LV20 (50 nM) showed proliferative effect, but OA-GL12 did not show on HaCaT cells from 2 to 10 pM. Data were presented as mean ± S.D., n =9. * P

    Article Snippet: Briefly, human keratinocytes (HaCaT) (KCB 200442YJ) and human skin fibroblasts (HSF) (KCB 200537) were provided by Conservation Genetics CAS Kunming Cell Bank of Kunming Institute of Zoology, the Chinese Academy of Sciences, and cultured in Dulbecco’s modified Eagle’s medium (DMEM, BI, Israel) with 10% FBS (BI, Israel) and penicillin (100 units/ml)–streptomycin (100 units/ml) in a humidified atmosphere of 5% CO2 (37°C).

    Techniques: Cell Culture, Transferring, Positive Control, Concentration Assay, Incubation

    Effect of OA-GL12 on the repair rate of HSF cell scratches HSF cells (2.5 × 10 5 /well) were cultured in 24-well plates for 12–24 h and then made to the mechanical scratch wound by a sterile pipette tip. After washing, cells were cultured for next periods in a serum-free basal medium (500 μl) with the continued presence of OA-GL12. The same volume of DMEM (serum free) was added as vehicle. ( A ) OA-GL12 (10 pM) illustrated distinct HSF cells scratch-healing activity. ( B ) OA-GL12 illustrated time- and concentration-dependent HSF cells scratch-healing activity. Contain cells were added into 96-well plates with basic DMEM (90 μl) without 10% FBS and incubated for 2–4 h. Afterward, OA-GL12 (10 μl) was added to each well and incubated for 24 h at various final concentrations then tested using a CellTiter 96® AQueous One Solution Assay. ( C , D ). OA-GL12 showed no impact on the proliferation of HSF and HUVEC cells from 2 pM to 10 nM. Data were presented as mean ± S.D., n =9. * P

    Journal: Bioscience Reports

    Article Title: A short peptide potentially promotes the healing of skin wound

    doi: 10.1042/BSR20181734

    Figure Lengend Snippet: Effect of OA-GL12 on the repair rate of HSF cell scratches HSF cells (2.5 × 10 5 /well) were cultured in 24-well plates for 12–24 h and then made to the mechanical scratch wound by a sterile pipette tip. After washing, cells were cultured for next periods in a serum-free basal medium (500 μl) with the continued presence of OA-GL12. The same volume of DMEM (serum free) was added as vehicle. ( A ) OA-GL12 (10 pM) illustrated distinct HSF cells scratch-healing activity. ( B ) OA-GL12 illustrated time- and concentration-dependent HSF cells scratch-healing activity. Contain cells were added into 96-well plates with basic DMEM (90 μl) without 10% FBS and incubated for 2–4 h. Afterward, OA-GL12 (10 μl) was added to each well and incubated for 24 h at various final concentrations then tested using a CellTiter 96® AQueous One Solution Assay. ( C , D ). OA-GL12 showed no impact on the proliferation of HSF and HUVEC cells from 2 pM to 10 nM. Data were presented as mean ± S.D., n =9. * P

    Article Snippet: Briefly, human keratinocytes (HaCaT) (KCB 200442YJ) and human skin fibroblasts (HSF) (KCB 200537) were provided by Conservation Genetics CAS Kunming Cell Bank of Kunming Institute of Zoology, the Chinese Academy of Sciences, and cultured in Dulbecco’s modified Eagle’s medium (DMEM, BI, Israel) with 10% FBS (BI, Israel) and penicillin (100 units/ml)–streptomycin (100 units/ml) in a humidified atmosphere of 5% CO2 (37°C).

    Techniques: Cell Culture, Transferring, Activity Assay, Concentration Assay, Incubation

    Photograph of an E12.5 embryo following culture for 28 h in DMEM/F12 and 20% FBS. Notice the head, divided brain vesicles, right forelimb bud and somites marked by arrows. Also note subcutaneous haemorrhage in the head region.

    Journal: Journal of Anatomy

    Article Title: A simple method of culture of 11.5-day-old rat embryos in DMEM/F12 and 20% fetal bovine serum

    doi: 10.1046/j.1469-7580.2003.00225.x

    Figure Lengend Snippet: Photograph of an E12.5 embryo following culture for 28 h in DMEM/F12 and 20% FBS. Notice the head, divided brain vesicles, right forelimb bud and somites marked by arrows. Also note subcutaneous haemorrhage in the head region.

    Article Snippet: The embryos from each dam were randomly assigned to two groups: 69 embryos were cultured on 90% heat-inactivated rat serum with 10% double distilled water and additional 1 mg mL−1 glucose (total of 2 mg mL−1 ); 85 embryos cultured on DMEM/F12 (50%/50%) supplemented with 20% FBS (all purchased from Biological Industries, Beth Haemek, Israel).

    Techniques:

    Photograph of an E12.5 embryo following culture for 28 h in DMEM/F12 and 20% FBS. Notice the head, divided brain vesicles, two branchial arches, forelimb bud, cardiac prominence and the attached open yolk sac. The arrowhead points to the optic vesicle.

    Journal: Journal of Anatomy

    Article Title: A simple method of culture of 11.5-day-old rat embryos in DMEM/F12 and 20% fetal bovine serum

    doi: 10.1046/j.1469-7580.2003.00225.x

    Figure Lengend Snippet: Photograph of an E12.5 embryo following culture for 28 h in DMEM/F12 and 20% FBS. Notice the head, divided brain vesicles, two branchial arches, forelimb bud, cardiac prominence and the attached open yolk sac. The arrowhead points to the optic vesicle.

    Article Snippet: The embryos from each dam were randomly assigned to two groups: 69 embryos were cultured on 90% heat-inactivated rat serum with 10% double distilled water and additional 1 mg mL−1 glucose (total of 2 mg mL−1 ); 85 embryos cultured on DMEM/F12 (50%/50%) supplemented with 20% FBS (all purchased from Biological Industries, Beth Haemek, Israel).

    Techniques:

    AR antagonist inhibited U87 proliferation in vitro and in vivo , but it had no effect on U251 ( A ) U251 and ( B ) U87 MTT assay. Cells cultured in phenol red-free DMEM containing 5% (v/v) charcoal stripped fetal bovine serum supplemented with 0.1% (v/v) DMSO or 10 nM R1881 or 10 μM ARN-509 or 10 μM MDV3100 (final concentration), error bar indicates standard deviation, ** P

    Journal: Oncotarget

    Article Title: Regulation of p53wt glioma cell proliferation by androgen receptor-mediated inhibition of small VCP/p97-interacting protein expression

    doi: 10.18632/oncotarget.15509

    Figure Lengend Snippet: AR antagonist inhibited U87 proliferation in vitro and in vivo , but it had no effect on U251 ( A ) U251 and ( B ) U87 MTT assay. Cells cultured in phenol red-free DMEM containing 5% (v/v) charcoal stripped fetal bovine serum supplemented with 0.1% (v/v) DMSO or 10 nM R1881 or 10 μM ARN-509 or 10 μM MDV3100 (final concentration), error bar indicates standard deviation, ** P

    Article Snippet: For treatment with R1881 or ARN-509 and MDV3100 (Enzalutamide, brand name Xtandi) both obtained from APExBio, TX, USA, cells were grown in phenol red-free DMEM (Gibco) medium supplemented with 10% (or indicated concentration, v/v) dextran-coated charcoal stripped FBS (Biological Industries, Israel) for 72 h prior to treatment.

    Techniques: In Vitro, In Vivo, MTT Assay, Cell Culture, Concentration Assay, Standard Deviation

    Mineralo-organic particles induce NET release by neutrophils. Mineralo-organic particles were prepared by adding 3 mM of CaCl 2 and NaH 2 PO 4 each in DMEM containing ( A ) 0.1% or ( B ) 3% FBS, prior to incubation and preparation for TEM without staining as described in Methods . Data are representative of three independent experiments. Scale bars: 200 nm. ( C ) Live cell imaging of human neutrophils stained with Hoechst 33342 (blue) and treated at time 0 with mineral particles (labeled with FITC-bovine serum albumin; FITC-BSA; green). NET-associated DNA was stained with Sytox (red). Data are representative of three independent experiments. Scale bars: 10 μm. Neutrophils were incubated for 2 hours in the absence ( D ) or presence of particles in 0.1% FBS ( E ), 0.12 mg/ml bovine serum fetuin-A (BSF) ( F ), or 40 mg/ml BSF ( G ). NET-associated DNA was stained green by Sytox. Data are representative of three independent experiments. Scale bars: 20 μm. ( H ) Percentage of neutrophils forming NETs 2 hours after addition of particles. Data are shown as means ± standard errors of the mean (SEM) and the results of at least three independent experiments. ** p

    Journal: Scientific Reports

    Article Title: Mineral particles stimulate innate immunity through neutrophil extracellular traps containing HMGB1

    doi: 10.1038/s41598-017-16778-4

    Figure Lengend Snippet: Mineralo-organic particles induce NET release by neutrophils. Mineralo-organic particles were prepared by adding 3 mM of CaCl 2 and NaH 2 PO 4 each in DMEM containing ( A ) 0.1% or ( B ) 3% FBS, prior to incubation and preparation for TEM without staining as described in Methods . Data are representative of three independent experiments. Scale bars: 200 nm. ( C ) Live cell imaging of human neutrophils stained with Hoechst 33342 (blue) and treated at time 0 with mineral particles (labeled with FITC-bovine serum albumin; FITC-BSA; green). NET-associated DNA was stained with Sytox (red). Data are representative of three independent experiments. Scale bars: 10 μm. Neutrophils were incubated for 2 hours in the absence ( D ) or presence of particles in 0.1% FBS ( E ), 0.12 mg/ml bovine serum fetuin-A (BSF) ( F ), or 40 mg/ml BSF ( G ). NET-associated DNA was stained green by Sytox. Data are representative of three independent experiments. Scale bars: 20 μm. ( H ) Percentage of neutrophils forming NETs 2 hours after addition of particles. Data are shown as means ± standard errors of the mean (SEM) and the results of at least three independent experiments. ** p

    Article Snippet: Preparation of mineralo-organic particles FBS (Biological Industries) for particle preparation was heat-inactivated and then filtered through 0.2-μm disc filters.

    Techniques: Incubation, Transmission Electron Microscopy, Staining, Live Cell Imaging, Labeling