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MAG induced ferroptosis in TNBC cells (A) Intracellular Fe 2+ levels in MAG-treated TNBC cells detected using the FerroOrange probe (B,C) Measurement of LPO and GSH levels in TNBC cells following MAG treatment (D) Protein expression levels of SLC7A11, GPX4, and DMT1 in MAG-treated TNBC cells (E) The morphological characteristics was observed by TEM after treatment with MAG (40 μM) for 24 h (F–I) Relative levels of Fe 2+ , LPO, GSH, and ROS in MDA-MB-231 cells following 2 h pretreatment <t>with</t> <t>Fer-1</t> or DFO, followed by MAG exposure (J) Western blotting of GPX4, SLC7A11, and DMT1 expression in TNBC cells pretreated with or without Fer-1 or DFO for 2 h, followed by MAG for 24 h * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
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MAG induced ferroptosis in TNBC cells (A) Intracellular Fe 2+ levels in MAG-treated TNBC cells detected using the FerroOrange probe (B,C) Measurement of LPO and GSH levels in TNBC cells following MAG treatment (D) Protein expression levels of SLC7A11, GPX4, and DMT1 in MAG-treated TNBC cells (E) The morphological characteristics was observed by TEM after treatment with MAG (40 μM) for 24 h (F–I) Relative levels of Fe 2+ , LPO, GSH, and ROS in MDA-MB-231 cells following 2 h pretreatment with Fer-1 or DFO, followed by MAG exposure (J) Western blotting of GPX4, SLC7A11, and DMT1 expression in TNBC cells pretreated with or without Fer-1 or DFO for 2 h, followed by MAG for 24 h * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Magnolol triggers ferroptosis in triple-negative breast cancer through modulation of the ETS2/SLC7A11/GPX4 signaling axis

doi: 10.3389/fphar.2026.1876648

Figure Lengend Snippet: MAG induced ferroptosis in TNBC cells (A) Intracellular Fe 2+ levels in MAG-treated TNBC cells detected using the FerroOrange probe (B,C) Measurement of LPO and GSH levels in TNBC cells following MAG treatment (D) Protein expression levels of SLC7A11, GPX4, and DMT1 in MAG-treated TNBC cells (E) The morphological characteristics was observed by TEM after treatment with MAG (40 μM) for 24 h (F–I) Relative levels of Fe 2+ , LPO, GSH, and ROS in MDA-MB-231 cells following 2 h pretreatment with Fer-1 or DFO, followed by MAG exposure (J) Western blotting of GPX4, SLC7A11, and DMT1 expression in TNBC cells pretreated with or without Fer-1 or DFO for 2 h, followed by MAG for 24 h * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: Ferrostatin-1 (Fer-1) and deferoxamine (DFO) were purchased from MedChemExpress (Shanghai, China).

Techniques: Expressing, Western Blot