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his tag  (ACROBiosystems)


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    Structured Review

    ACROBiosystems his tag
    His Tag, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc6-c52h9-50ug/Cynomolgus+Fc+gamma+RIII+%2F+CD16+Protein%2C+His+Tag/pmc12380559-64-36-38
    Average 95 stars, based on 1 article reviews
    his tag - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Binding Assay:

    Article Title: PD-1 Bispecific Killer Engager (PD-1 BiKE) effectively depletes effector T lymphocytes in experimental autoimmune encephalomyelitis
    Article Snippet: Flow cytometry (BD FACS Canto) was used to detect BiKE-bound cells. .. For simultaneous binding to PD-1 + cells and CD16, PD-1 + cells were incubated with varying concentrations of PD-1 BiKE for 30 min in PBS at 4°C, washed, then 150 nM CD16 soluble protein carrying a His tag (AcroBiosystems, cat. CDA-M52H8) was added for 30 min. After washing, anti-His-APC antibody (BioLegend, cat. 36205) was added to cells to detect CD16-bound cells; cells were washed, then flow cytometry was used to determine the quantity of cells bound by PD-1 BiKE and CD16 protein. .. For simultaneous binding to CD16 + cells and PD-1, NK cells were incubated with varying concentrations of PD-1 BiKE for 30 min in PBS at 4°C, washed, then 150 nM PD-1 soluble protein carrying a His tag (AcroBiosystems, cat. PD1-M52H3) was added for 30 min. After washing, anti-His-APC antibody (BioLegend, cat. 36205) was added to cells to detect PD-1-bound cells, cells were washed, then flow cytometry was used to determine the quantity of cells bound by PD-1 BiKE and PD-1 protein.

    Incubation:

    Article Title: PD-1 Bispecific Killer Engager (PD-1 BiKE) effectively depletes effector T lymphocytes in experimental autoimmune encephalomyelitis
    Article Snippet: Flow cytometry (BD FACS Canto) was used to detect BiKE-bound cells. .. For simultaneous binding to PD-1 + cells and CD16, PD-1 + cells were incubated with varying concentrations of PD-1 BiKE for 30 min in PBS at 4°C, washed, then 150 nM CD16 soluble protein carrying a His tag (AcroBiosystems, cat. CDA-M52H8) was added for 30 min. After washing, anti-His-APC antibody (BioLegend, cat. 36205) was added to cells to detect CD16-bound cells; cells were washed, then flow cytometry was used to determine the quantity of cells bound by PD-1 BiKE and CD16 protein. .. For simultaneous binding to CD16 + cells and PD-1, NK cells were incubated with varying concentrations of PD-1 BiKE for 30 min in PBS at 4°C, washed, then 150 nM PD-1 soluble protein carrying a His tag (AcroBiosystems, cat. PD1-M52H3) was added for 30 min. After washing, anti-His-APC antibody (BioLegend, cat. 36205) was added to cells to detect PD-1-bound cells, cells were washed, then flow cytometry was used to determine the quantity of cells bound by PD-1 BiKE and PD-1 protein.

    Flow Cytometry:

    Article Title: PD-1 Bispecific Killer Engager (PD-1 BiKE) effectively depletes effector T lymphocytes in experimental autoimmune encephalomyelitis
    Article Snippet: Flow cytometry (BD FACS Canto) was used to detect BiKE-bound cells. .. For simultaneous binding to PD-1 + cells and CD16, PD-1 + cells were incubated with varying concentrations of PD-1 BiKE for 30 min in PBS at 4°C, washed, then 150 nM CD16 soluble protein carrying a His tag (AcroBiosystems, cat. CDA-M52H8) was added for 30 min. After washing, anti-His-APC antibody (BioLegend, cat. 36205) was added to cells to detect CD16-bound cells; cells were washed, then flow cytometry was used to determine the quantity of cells bound by PD-1 BiKE and CD16 protein. .. For simultaneous binding to CD16 + cells and PD-1, NK cells were incubated with varying concentrations of PD-1 BiKE for 30 min in PBS at 4°C, washed, then 150 nM PD-1 soluble protein carrying a His tag (AcroBiosystems, cat. PD1-M52H3) was added for 30 min. After washing, anti-His-APC antibody (BioLegend, cat. 36205) was added to cells to detect PD-1-bound cells, cells were washed, then flow cytometry was used to determine the quantity of cells bound by PD-1 BiKE and PD-1 protein.



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    his tag  (ACROBiosystems)
    95
    ACROBiosystems his tag
    His Tag, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc6-c52h9-50ug/Cynomolgus+Fc+gamma+RIII+%2F+CD16+Protein%2C+His+Tag/pmc12380559-64-36-38
    Average 95 stars, based on 1 article reviews
    his tag - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

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