Structured Review

GeneTex fbln2
Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified <t>Fbln2</t> as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
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Images

1) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

2) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

3) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

4) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

5) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

6) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

7) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

8) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

9) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

10) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

11) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

12) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

13) Product Images from "Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling"

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

doi: 10.1161/JAHA.113.000078

Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
Figure Legend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

Techniques Used: Sequencing, Luciferase, Cotransfection, Plasmid Preparation

Related Articles

Clone Assay:

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. A short (≈250 bp) fragment from the 3′‐UTR of Fbln2 was cloned downstream of the stop codon of the Renilla luciferase in a dual‐luciferase reporter vector. .. In parallel, a fragment of the Ncx1 3′‐UTRs predicted to contain a conserved “seed” sequence complementary to miR‐1 was also cloned, serving a positive control for the luciferase assay.

In Vivo:

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. In addition, we evaluated whether Fbln2 and Ncx1 expression was regulated by miR‐1 in vivo in the setting of pressure‐overload–induced hypertrophy. .. Western blot analysis demonstrated that at the protein level, the expression of both Fbln2 and Ncx1 was significantly decreased in the LV tissue of the AAV9.miR‐1 – compared with AAV9.GFP –treated animals ( C through E).

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. Taken together, these results suggest that the restoration of miR‐1 levels in the setting of pressure‐overload–induced hypertrophy in vivo was paralleled by the modulation of a novel direct target gene, Fbln2, as well as the previously identified targets, Ncx1 , Igf1 , Twf1 , and Calm2 . .. Discussion Recent studies have shown that changes in miRNA expression play an important role in diverse aspects of cardiovascular pathophysiology, and the modulation of miRNA activity could provide potential new therapeutic targets for cardiovascular diseases., The ability of a single miRNA to control the expression of hundreds of proteins – suggests that modulation of individual miRNAs can influence multiple pathways simultaneously.

Luciferase:

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. We performed a luciferase‐based expression assays in vitro to verify that Fbln2 is a bona fide miR‐1 target. .. A short (≈250 bp) fragment from the 3′‐UTR of Fbln2 was cloned downstream of the stop codon of the Renilla luciferase in a dual‐luciferase reporter vector.

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. A short (≈250 bp) fragment from the 3′‐UTR of Fbln2 was cloned downstream of the stop codon of the Renilla luciferase in a dual‐luciferase reporter vector. .. In parallel, a fragment of the Ncx1 3′‐UTRs predicted to contain a conserved “seed” sequence complementary to miR‐1 was also cloned, serving a positive control for the luciferase assay.

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. In the presence of miR‐1, we observed a significant decrease in luciferase activity of Fbln2 and Ncx1 constructs ( B). ..

In Vitro:

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. We performed a luciferase‐based expression assays in vitro to verify that Fbln2 is a bona fide miR‐1 target. .. A short (≈250 bp) fragment from the 3′‐UTR of Fbln2 was cloned downstream of the stop codon of the Renilla luciferase in a dual‐luciferase reporter vector.

Construct:

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. In the presence of miR‐1, we observed a significant decrease in luciferase activity of Fbln2 and Ncx1 constructs ( B). ..

other:

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: Using a target prediction algorithm, we identified Fbln2 as putative target gene of miR‐1 .

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: Fbln2 is an extracellular matrix protein that is highly expressed in the developing heart and plays an important role during embryonic development and cardiac remodeling.

Activity Assay:

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. In the presence of miR‐1, we observed a significant decrease in luciferase activity of Fbln2 and Ncx1 constructs ( B). ..

Expressing:

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. Consistent with the postulated role of Fbln2 as a key pro fibrotic factor, we showed that the restoration of miR‐1 expression significantly decreased its expression as well as the marked reduction in collagen content and the downregulation of key profibrotic factors. .. The significance of this observation is strengthened by data from human samples showing that Fbln2 protein expression is significantly increased in hypertrophic hearts ( J), suggesting that it may be a key player in the development of heart failure in humans.

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. In addition, we evaluated whether Fbln2 and Ncx1 expression was regulated by miR‐1 in vivo in the setting of pressure‐overload–induced hypertrophy. .. Western blot analysis demonstrated that at the protein level, the expression of both Fbln2 and Ncx1 was significantly decreased in the LV tissue of the AAV9.miR‐1 – compared with AAV9.GFP –treated animals ( C through E).

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. We performed a luciferase‐based expression assays in vitro to verify that Fbln2 is a bona fide miR‐1 target. .. A short (≈250 bp) fragment from the 3′‐UTR of Fbln2 was cloned downstream of the stop codon of the Renilla luciferase in a dual‐luciferase reporter vector.

Plasmid Preparation:

Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling
Article Snippet: .. A short (≈250 bp) fragment from the 3′‐UTR of Fbln2 was cloned downstream of the stop codon of the Renilla luciferase in a dual‐luciferase reporter vector. .. In parallel, a fragment of the Ncx1 3′‐UTRs predicted to contain a conserved “seed” sequence complementary to miR‐1 was also cloned, serving a positive control for the luciferase assay.

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    GeneTex fbln2
    Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified <t>Fbln2</t> as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P
    Fbln2, supplied by GeneTex, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Therapeutic Cardiac-Targeted Delivery of miR‐1 Reverses Pressure Overload-Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

    doi: 10.1161/JAHA.113.000078

    Figure Lengend Snippet: Identification and validation of the miR‐1 direct target genes. A, Bioinformatics analysis identified Fbln2 as a putative target gene of miR‐1 . The mRNA sequence of Fbln2 is predicted to contain a conserved “seed” sequence complimentary to miR‐1 in the 3′‐untranslated region (3′‐UTR). B, Luciferase reporter assays performed by cotransfection of miR‐1 “mimics” (pre– miR‐1 ) with a luciferase vector linked to the Fbln2 or Ncx1 3′‐UTR or deleted 3′‐UTR complementary site (No 3′‐UTR); a control nontargeting “mimic” (pre–miR‐control) was also included. Data represent mean±SE of n=6 experiments per condition, $ P

    Article Snippet: We performed a luciferase‐based expression assays in vitro to verify that Fbln2 is a bona fide miR‐1 target.

    Techniques: Sequencing, Luciferase, Cotransfection, Plasmid Preparation