fastdigest psti  (Thermo Fisher)


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    Name:
    FastDigest XbaI
    Description:
    5 T ↓C T A G A 3 3 A G A T C ↑T 5 Thermo Scientific FastDigest XbaI restriction enzyme recognizes T CTAGA site and cuts best at 37°C in 5 15 minutes using universal FastDigest Buffer Thermo Scientific FastDigest XbaI is one of an advanced line of fast restriction enzymes that are all 100 active in the universal FastDigest and FastDigest Green reaction buffers The universal buffer allows rapid single double or multiple DNA digestion within 5 15 minutes eliminating any need for buffer change or subsequent DNA clean up steps See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity heat inactivation and incubation times for this and other FastDigest restriction enzymes DNA modifying enzymes such as Klenow Fragment T4 DNA Ligase alkaline phosphatases and T4 DNA Polymerase all have 100 activity in FastDigest Buffer Therefore enzymes for downstream applications can be directly added to the FastDigest reaction mix For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects Features• 100 activity of all FastDigest enzymes in the universal buffer• 100 buffer compatibility with downstream applications• Complete digestion in 5 15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes availableApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern Blot• Restriction fragment length polymorphism RFLP • SNP analysisNote The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer For applications that require product analysis by fluorescence excitation e g concentration measurements in UV light the colorless FastDigest Buffer is recommended For methylation sensitivity refer to product specifications
    Catalog Number:
    fd0684
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher fastdigest psti
    5 T ↓C T A G A 3 3 A G A T C ↑T 5 Thermo Scientific FastDigest XbaI restriction enzyme recognizes T CTAGA site and cuts best at 37°C in 5 15 minutes using universal FastDigest Buffer Thermo Scientific FastDigest XbaI is one of an advanced line of fast restriction enzymes that are all 100 active in the universal FastDigest and FastDigest Green reaction buffers The universal buffer allows rapid single double or multiple DNA digestion within 5 15 minutes eliminating any need for buffer change or subsequent DNA clean up steps See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity heat inactivation and incubation times for this and other FastDigest restriction enzymes DNA modifying enzymes such as Klenow Fragment T4 DNA Ligase alkaline phosphatases and T4 DNA Polymerase all have 100 activity in FastDigest Buffer Therefore enzymes for downstream applications can be directly added to the FastDigest reaction mix For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects Features• 100 activity of all FastDigest enzymes in the universal buffer• 100 buffer compatibility with downstream applications• Complete digestion in 5 15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes availableApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern Blot• Restriction fragment length polymorphism RFLP • SNP analysisNote The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer For applications that require product analysis by fluorescence excitation e g concentration measurements in UV light the colorless FastDigest Buffer is recommended For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/fastdigest psti/product/Thermo Fisher
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fastdigest psti - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Interferon lambda 4 signals via the IFNλ receptor to regulate antiviral activity against HCV and coronaviruses
    Article Snippet: .. The following PCR programme was used 1: 95°C for 5 min 2: 30 cycles of 95°C for 1 min, 59°C for 1 min and 72°C for 1 min and 45 s 3: 72°C for 7 min. All the constructs were cloned into the pEF2 vector using Fastdigest Kpn I (Thermo Scientific, catalogue number FD0524) and Fastdigest XbaI (Thermo Scientific, catalogue number FD0684) following the manufacturer's instructions. .. The IFNλ4 mutant IFNλ4 N61D was generated by site-directed mutagenesis using IFNλ4_FLAG in the pEF2 vector as a template.

    Article Title: Translation, but not transfection limits clinically relevant, exogenous mRNA based induction of alpha-4 integrin expression on human mesenchymal stem cells
    Article Snippet: .. mRNA synthesis in vitro In order to produce mRNA in vitro , a fragment containing itga4 gene cDNA was released from the pITGA4 vector by simultaneous XbaI (FD0684, ThermoScientific) and XhoI (FD0694, ThermoScientific) digestion before being cloned into the pSP72 vector with T4 DNA ligase (EL0014, ThermoScientific). .. This newly created pITGA4-SP72 vector was sequenced (ABI3730 Genetic Analyzer, Applied Biosystems) and used as a template for the mRNA production in vitro .

    Article Title: Design, Construction, and Validation of Histone-Binding Effectors in vitro and in Cells
    Article Snippet: .. Incubate the streak plate overnight at 37 °C. (4) Perform PCR as follows: 95 °C for 2 min; 25 cycles of 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 1 min; 72 °C for 5 min; and held at 4 °C. (5) Analyze 20−50% of each reaction mixture via gel electrophoresis. (6) Inoculate 5 mL of liquid LB, 100 μ g/mL ampicillin cultures with candidate clones from the streak plate, grow for 18 h at 37 °C with shaking, and miniprep the plasmid DNA (Sigma PLN350–1KT). (7) Perform restriction digests as follows: 500−1000 ng of plasmid DNA, 1 μ L of each of FastDigest enzymes XbaI and Pst I, and 1× FastDigest buffer (Thermo Fisher FD0684, FD0614, and B72). .. Analyze the products using standard agarose gel electrophoresis with TAE buffer.

    In Vitro:

    Article Title: Translation, but not transfection limits clinically relevant, exogenous mRNA based induction of alpha-4 integrin expression on human mesenchymal stem cells
    Article Snippet: .. mRNA synthesis in vitro In order to produce mRNA in vitro , a fragment containing itga4 gene cDNA was released from the pITGA4 vector by simultaneous XbaI (FD0684, ThermoScientific) and XhoI (FD0694, ThermoScientific) digestion before being cloned into the pSP72 vector with T4 DNA ligase (EL0014, ThermoScientific). .. This newly created pITGA4-SP72 vector was sequenced (ABI3730 Genetic Analyzer, Applied Biosystems) and used as a template for the mRNA production in vitro .

    Nucleic Acid Electrophoresis:

    Article Title: Design, Construction, and Validation of Histone-Binding Effectors in vitro and in Cells
    Article Snippet: .. Incubate the streak plate overnight at 37 °C. (4) Perform PCR as follows: 95 °C for 2 min; 25 cycles of 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 1 min; 72 °C for 5 min; and held at 4 °C. (5) Analyze 20−50% of each reaction mixture via gel electrophoresis. (6) Inoculate 5 mL of liquid LB, 100 μ g/mL ampicillin cultures with candidate clones from the streak plate, grow for 18 h at 37 °C with shaking, and miniprep the plasmid DNA (Sigma PLN350–1KT). (7) Perform restriction digests as follows: 500−1000 ng of plasmid DNA, 1 μ L of each of FastDigest enzymes XbaI and Pst I, and 1× FastDigest buffer (Thermo Fisher FD0684, FD0614, and B72). .. Analyze the products using standard agarose gel electrophoresis with TAE buffer.

    Construct:

    Article Title: Interferon lambda 4 signals via the IFNλ receptor to regulate antiviral activity against HCV and coronaviruses
    Article Snippet: .. The following PCR programme was used 1: 95°C for 5 min 2: 30 cycles of 95°C for 1 min, 59°C for 1 min and 72°C for 1 min and 45 s 3: 72°C for 7 min. All the constructs were cloned into the pEF2 vector using Fastdigest Kpn I (Thermo Scientific, catalogue number FD0524) and Fastdigest XbaI (Thermo Scientific, catalogue number FD0684) following the manufacturer's instructions. .. The IFNλ4 mutant IFNλ4 N61D was generated by site-directed mutagenesis using IFNλ4_FLAG in the pEF2 vector as a template.

    Purification:

    Article Title: Design, Construction, and Validation of Histone-Binding Effectors in vitro and in Cells
    Article Snippet: .. Restriction digestion followed by electrophoresis (1% agarose in TAE) was used to analyze purified plasmid DNA (Sigma PLN350–1KT): 500−1000 ng of plasmid DNA, 1 μ L each of FastDigest enzymes Xba I and PstI, and 1× FastDigest buffer (Thermo Fisher FD0684, FD0614, and B72). .. HEK293 Gal4-EED/luc cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% tetracycline-free fetal bovine serum and 1% penicillin and streptomycin (pen/strep) at 37 °C in a humidified CO2 incubator.

    Electrophoresis:

    Article Title: Design, Construction, and Validation of Histone-Binding Effectors in vitro and in Cells
    Article Snippet: .. Restriction digestion followed by electrophoresis (1% agarose in TAE) was used to analyze purified plasmid DNA (Sigma PLN350–1KT): 500−1000 ng of plasmid DNA, 1 μ L each of FastDigest enzymes Xba I and PstI, and 1× FastDigest buffer (Thermo Fisher FD0684, FD0614, and B72). .. HEK293 Gal4-EED/luc cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% tetracycline-free fetal bovine serum and 1% penicillin and streptomycin (pen/strep) at 37 °C in a humidified CO2 incubator.

    Polymerase Chain Reaction:

    Article Title: Interferon lambda 4 signals via the IFNλ receptor to regulate antiviral activity against HCV and coronaviruses
    Article Snippet: .. The following PCR programme was used 1: 95°C for 5 min 2: 30 cycles of 95°C for 1 min, 59°C for 1 min and 72°C for 1 min and 45 s 3: 72°C for 7 min. All the constructs were cloned into the pEF2 vector using Fastdigest Kpn I (Thermo Scientific, catalogue number FD0524) and Fastdigest XbaI (Thermo Scientific, catalogue number FD0684) following the manufacturer's instructions. .. The IFNλ4 mutant IFNλ4 N61D was generated by site-directed mutagenesis using IFNλ4_FLAG in the pEF2 vector as a template.

    Article Title: Design, Construction, and Validation of Histone-Binding Effectors in vitro and in Cells
    Article Snippet: .. Incubate the streak plate overnight at 37 °C. (4) Perform PCR as follows: 95 °C for 2 min; 25 cycles of 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 1 min; 72 °C for 5 min; and held at 4 °C. (5) Analyze 20−50% of each reaction mixture via gel electrophoresis. (6) Inoculate 5 mL of liquid LB, 100 μ g/mL ampicillin cultures with candidate clones from the streak plate, grow for 18 h at 37 °C with shaking, and miniprep the plasmid DNA (Sigma PLN350–1KT). (7) Perform restriction digests as follows: 500−1000 ng of plasmid DNA, 1 μ L of each of FastDigest enzymes XbaI and Pst I, and 1× FastDigest buffer (Thermo Fisher FD0684, FD0614, and B72). .. Analyze the products using standard agarose gel electrophoresis with TAE buffer.

    Plasmid Preparation:

    Article Title: Design, Construction, and Validation of Histone-Binding Effectors in vitro and in Cells
    Article Snippet: .. Restriction digestion followed by electrophoresis (1% agarose in TAE) was used to analyze purified plasmid DNA (Sigma PLN350–1KT): 500−1000 ng of plasmid DNA, 1 μ L each of FastDigest enzymes Xba I and PstI, and 1× FastDigest buffer (Thermo Fisher FD0684, FD0614, and B72). .. HEK293 Gal4-EED/luc cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% tetracycline-free fetal bovine serum and 1% penicillin and streptomycin (pen/strep) at 37 °C in a humidified CO2 incubator.

    Article Title: Interferon lambda 4 signals via the IFNλ receptor to regulate antiviral activity against HCV and coronaviruses
    Article Snippet: .. The following PCR programme was used 1: 95°C for 5 min 2: 30 cycles of 95°C for 1 min, 59°C for 1 min and 72°C for 1 min and 45 s 3: 72°C for 7 min. All the constructs were cloned into the pEF2 vector using Fastdigest Kpn I (Thermo Scientific, catalogue number FD0524) and Fastdigest XbaI (Thermo Scientific, catalogue number FD0684) following the manufacturer's instructions. .. The IFNλ4 mutant IFNλ4 N61D was generated by site-directed mutagenesis using IFNλ4_FLAG in the pEF2 vector as a template.

    Article Title: Amdinocillin (Mecillinam) Resistance Mutations in Clinical Isolates and Laboratory-Selected Mutants of Escherichia coli
    Article Snippet: .. The insert and the plasmid were first cleaved with Fast Digest XbaI and PstI according to the manual provided by Thermo Fisher and then ligated with Ready-To-Go T4 DNA ligase (GE Health Care Life Sciences). .. The resulting plasmids were transformed into MG1655 by electroporation and then spread on Cam-supplemented MH agar plates for selection of plasmid uptake.

    Article Title: Translation, but not transfection limits clinically relevant, exogenous mRNA based induction of alpha-4 integrin expression on human mesenchymal stem cells
    Article Snippet: .. mRNA synthesis in vitro In order to produce mRNA in vitro , a fragment containing itga4 gene cDNA was released from the pITGA4 vector by simultaneous XbaI (FD0684, ThermoScientific) and XhoI (FD0694, ThermoScientific) digestion before being cloned into the pSP72 vector with T4 DNA ligase (EL0014, ThermoScientific). .. This newly created pITGA4-SP72 vector was sequenced (ABI3730 Genetic Analyzer, Applied Biosystems) and used as a template for the mRNA production in vitro .

    Article Title: Design, Construction, and Validation of Histone-Binding Effectors in vitro and in Cells
    Article Snippet: .. Incubate the streak plate overnight at 37 °C. (4) Perform PCR as follows: 95 °C for 2 min; 25 cycles of 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 1 min; 72 °C for 5 min; and held at 4 °C. (5) Analyze 20−50% of each reaction mixture via gel electrophoresis. (6) Inoculate 5 mL of liquid LB, 100 μ g/mL ampicillin cultures with candidate clones from the streak plate, grow for 18 h at 37 °C with shaking, and miniprep the plasmid DNA (Sigma PLN350–1KT). (7) Perform restriction digests as follows: 500−1000 ng of plasmid DNA, 1 μ L of each of FastDigest enzymes XbaI and Pst I, and 1× FastDigest buffer (Thermo Fisher FD0684, FD0614, and B72). .. Analyze the products using standard agarose gel electrophoresis with TAE buffer.

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  • 95
    Thermo Fisher fastdigest psti
    Fastdigest Psti, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest psti/product/Thermo Fisher
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fastdigest psti - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    94
    Thermo Fisher fastdigest psti restriction enzyme
    Fastdigest Psti Restriction Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest psti restriction enzyme/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fastdigest psti restriction enzyme - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    98
    Thermo Fisher psti restriction enzymes
    Psti Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psti restriction enzymes/product/Thermo Fisher
    Average 98 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    psti restriction enzymes - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

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