fastdigest ncoi  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    FastDigest NcoI
    Description:
    5 C ↓C A T G G 3 3 G G T A C ↑C 5 Thermo Scientific FastDigest NcoI restriction enzyme recognizes C CATGG site and cuts best at 37°C in 5 15 minutes using universal FastDigest Buffer Isoschizomers Bsp19I Thermo Scientific FastDigest NcoI is one of an advanced line of fast restriction enzymes that are all 100 active in the universal FastDigest and FastDigest Green reaction buffers The universal buffer allows rapid single double or multiple DNA digestion within 5 15 minutes eliminating any need for buffer change or subsequent DNA clean up steps See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity heat inactivation and incubation times for this and other FastDigest restriction enzymes DNA modifying enzymes such as Klenow Fragment T4 DNA Ligase alkaline phosphatases and T4 DNA Polymerase all have 100 activity in FastDigest Buffer Therefore enzymes for downstream applications can be directly added to the FastDigest reaction mix For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects Features• 100 activity of all FastDigest enzymes in the universal buffer• 100 buffer compatibility with downstream applications• Complete digestion in 5 15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes availableApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern Blot• Restriction fragment length polymorphism RFLP • SNP analysisNote The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer For applications that require product analysis by fluorescence excitation e g concentration measurements in UV light the colorless FastDigest Buffer is recommended For methylation sensitivity refer to product specifications
    Catalog Number:
    fd0573
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher fastdigest ncoi
    5 C ↓C A T G G 3 3 G G T A C ↑C 5 Thermo Scientific FastDigest NcoI restriction enzyme recognizes C CATGG site and cuts best at 37°C in 5 15 minutes using universal FastDigest Buffer Isoschizomers Bsp19I Thermo Scientific FastDigest NcoI is one of an advanced line of fast restriction enzymes that are all 100 active in the universal FastDigest and FastDigest Green reaction buffers The universal buffer allows rapid single double or multiple DNA digestion within 5 15 minutes eliminating any need for buffer change or subsequent DNA clean up steps See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity heat inactivation and incubation times for this and other FastDigest restriction enzymes DNA modifying enzymes such as Klenow Fragment T4 DNA Ligase alkaline phosphatases and T4 DNA Polymerase all have 100 activity in FastDigest Buffer Therefore enzymes for downstream applications can be directly added to the FastDigest reaction mix For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects Features• 100 activity of all FastDigest enzymes in the universal buffer• 100 buffer compatibility with downstream applications• Complete digestion in 5 15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes availableApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern Blot• Restriction fragment length polymorphism RFLP • SNP analysisNote The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer For applications that require product analysis by fluorescence excitation e g concentration measurements in UV light the colorless FastDigest Buffer is recommended For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/fastdigest ncoi/product/Thermo Fisher
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    fastdigest ncoi - by Bioz Stars, 2020-07
    94/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: BtsCI and BseGI display sequence preference in the nucleotides flanking the recognition sequence
    Article Snippet: .. We digest the amplicon and pBAD-Ara::cmyc-His with FastDigest NcoI and FastDigest BgIII (both ThermoFischer Scientific) for 30min in Fast Digest buffer according to the supplier’s double digestion protocol. .. We treated the vector with alkaline phosphatase (NEB) at 37°C for 1h to avoid recircularization.

    Article Title: Tumour necrosis factor gene polymorphisms in Egyptian patients with rheumatoid arthritis and their relation to disease activity and severity
    Article Snippet: .. The PCR cycling conditions were performed according to the protocol described by Cabrara et al. [ ] with a slight modification: initial denaturation at 95°C for 5 minutes followed by 35 cycles of amplification: 94°C for 45 sec., 62.5°C for 45 sec, and a final extension at 72°C for 1.5 min. PCR products were digested (at 37°C) by NcoI (Thermo Scientific Cat. no. FD0574) according to the manufacturer’s protocol. .. Digested PCR products were electrophoresed in 4% agarose gel stained with ethidium bromide and followed by ultraviolet visualisation.

    In Vitro:

    Article Title: Characterization of a novel zebrafish (Danio rerio) gene, wdr81, associated with cerebellar ataxia, mental retardation and dysequilibrium syndrome (CAMRQ)
    Article Snippet: .. NcoI (FD0573, Thermo Fisher Scientific) and ApaI (ER1411, Thermo Fisher Scientific) were utilized for linearization of the plasmid construct and the sense RNA probe was made using the SP6 enzyme mix (AM1320, MaxiScript SP6/T7 In Vitro Transcription Kit, Ambion, USA) and DIG-labelling mix (11277073910, DIG RNA Labelling Mix, Roche, Germany). .. The slides were visualized with the brightfield upright microscope (Fluorescent and DIC equipped upright microscope, Zeiss, Germany).

    Ligation:

    Article Title: Genetically engineered probiotic for the treatment of phenylketonuria (PKU); assessment of a novel treatment in vitro and in the PAHenu2 mouse model of PKU
    Article Snippet: .. Desired ligation of the plasmid in each colony was determined from extracted plasmid (Ultraclean 6 Minute Mini Plasmid Prep Kit, MoBio, USA) by restriction digest with FastDigest enzymes NcoI and SalI (Thermo Scientific, USA) and visualization on ethidium bromide gel. .. The clone with successful insertion, pSLERGT, was sequence verified by BioBasic Inc. (Canada).

    Article Title: Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves. Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves
    Article Snippet: .. Upon confirmation of the recombinants with blue‐white colony screening and colony PCR, the easI gene was excised from the plasmid by digestion with FastDigest NcoI and BamHI (Thermo Scientific, USA), followed by ligation with linearized overexpression vector, pET28a (Novagen, Germany) to produce pET28a‐ easI . .. The DNA sequence of the constructed plasmids was verified by automated Sanger sequencing.

    Size-exclusion Chromatography:

    Article Title: Tumour necrosis factor gene polymorphisms in Egyptian patients with rheumatoid arthritis and their relation to disease activity and severity
    Article Snippet: .. The PCR cycling conditions were performed according to the protocol described by Cabrara et al. [ ] with a slight modification: initial denaturation at 95°C for 5 minutes followed by 35 cycles of amplification: 94°C for 45 sec., 62.5°C for 45 sec, and a final extension at 72°C for 1.5 min. PCR products were digested (at 37°C) by NcoI (Thermo Scientific Cat. no. FD0574) according to the manufacturer’s protocol. .. Digested PCR products were electrophoresed in 4% agarose gel stained with ethidium bromide and followed by ultraviolet visualisation.

    Construct:

    Article Title: Characterization of a novel zebrafish (Danio rerio) gene, wdr81, associated with cerebellar ataxia, mental retardation and dysequilibrium syndrome (CAMRQ)
    Article Snippet: .. NcoI (FD0573, Thermo Fisher Scientific) and ApaI (ER1411, Thermo Fisher Scientific) were utilized for linearization of the plasmid construct and the sense RNA probe was made using the SP6 enzyme mix (AM1320, MaxiScript SP6/T7 In Vitro Transcription Kit, Ambion, USA) and DIG-labelling mix (11277073910, DIG RNA Labelling Mix, Roche, Germany). .. The slides were visualized with the brightfield upright microscope (Fluorescent and DIC equipped upright microscope, Zeiss, Germany).

    Colony Assay:

    Article Title: Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves. Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves
    Article Snippet: .. Upon confirmation of the recombinants with blue‐white colony screening and colony PCR, the easI gene was excised from the plasmid by digestion with FastDigest NcoI and BamHI (Thermo Scientific, USA), followed by ligation with linearized overexpression vector, pET28a (Novagen, Germany) to produce pET28a‐ easI . .. The DNA sequence of the constructed plasmids was verified by automated Sanger sequencing.

    Polymerase Chain Reaction:

    Article Title: A Specific Blood Signature Reveals Higher Levels of S100A12: A Potential Bladder Cancer Diagnostic Biomarker Along With Urinary Engrailed-2 Protein Detection
    Article Snippet: .. The ends of the PCR product were cleaved using FastDigest restriction endonucleases NcoI and HindIII (Thermo Scientific). .. By ligation to IPTG-inducible expression vector pLEXWO481 digested before with the same enzymes set the S100A12 reading frame was fused in frame with the N-terminal His-tag.

    Article Title: Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves. Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves
    Article Snippet: .. Upon confirmation of the recombinants with blue‐white colony screening and colony PCR, the easI gene was excised from the plasmid by digestion with FastDigest NcoI and BamHI (Thermo Scientific, USA), followed by ligation with linearized overexpression vector, pET28a (Novagen, Germany) to produce pET28a‐ easI . .. The DNA sequence of the constructed plasmids was verified by automated Sanger sequencing.

    Article Title: Tumour necrosis factor gene polymorphisms in Egyptian patients with rheumatoid arthritis and their relation to disease activity and severity
    Article Snippet: .. The PCR cycling conditions were performed according to the protocol described by Cabrara et al. [ ] with a slight modification: initial denaturation at 95°C for 5 minutes followed by 35 cycles of amplification: 94°C for 45 sec., 62.5°C for 45 sec, and a final extension at 72°C for 1.5 min. PCR products were digested (at 37°C) by NcoI (Thermo Scientific Cat. no. FD0574) according to the manufacturer’s protocol. .. Digested PCR products were electrophoresed in 4% agarose gel stained with ethidium bromide and followed by ultraviolet visualisation.

    Modification:

    Article Title: Tumour necrosis factor gene polymorphisms in Egyptian patients with rheumatoid arthritis and their relation to disease activity and severity
    Article Snippet: .. The PCR cycling conditions were performed according to the protocol described by Cabrara et al. [ ] with a slight modification: initial denaturation at 95°C for 5 minutes followed by 35 cycles of amplification: 94°C for 45 sec., 62.5°C for 45 sec, and a final extension at 72°C for 1.5 min. PCR products were digested (at 37°C) by NcoI (Thermo Scientific Cat. no. FD0574) according to the manufacturer’s protocol. .. Digested PCR products were electrophoresed in 4% agarose gel stained with ethidium bromide and followed by ultraviolet visualisation.

    Over Expression:

    Article Title: Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves. Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves
    Article Snippet: .. Upon confirmation of the recombinants with blue‐white colony screening and colony PCR, the easI gene was excised from the plasmid by digestion with FastDigest NcoI and BamHI (Thermo Scientific, USA), followed by ligation with linearized overexpression vector, pET28a (Novagen, Germany) to produce pET28a‐ easI . .. The DNA sequence of the constructed plasmids was verified by automated Sanger sequencing.

    Plasmid Preparation:

    Article Title: Genetically engineered probiotic for the treatment of phenylketonuria (PKU); assessment of a novel treatment in vitro and in the PAHenu2 mouse model of PKU
    Article Snippet: .. Desired ligation of the plasmid in each colony was determined from extracted plasmid (Ultraclean 6 Minute Mini Plasmid Prep Kit, MoBio, USA) by restriction digest with FastDigest enzymes NcoI and SalI (Thermo Scientific, USA) and visualization on ethidium bromide gel. .. The clone with successful insertion, pSLERGT, was sequence verified by BioBasic Inc. (Canada).

    Article Title: Characterization of a novel zebrafish (Danio rerio) gene, wdr81, associated with cerebellar ataxia, mental retardation and dysequilibrium syndrome (CAMRQ)
    Article Snippet: .. NcoI (FD0573, Thermo Fisher Scientific) and ApaI (ER1411, Thermo Fisher Scientific) were utilized for linearization of the plasmid construct and the sense RNA probe was made using the SP6 enzyme mix (AM1320, MaxiScript SP6/T7 In Vitro Transcription Kit, Ambion, USA) and DIG-labelling mix (11277073910, DIG RNA Labelling Mix, Roche, Germany). .. The slides were visualized with the brightfield upright microscope (Fluorescent and DIC equipped upright microscope, Zeiss, Germany).

    Article Title: Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves. Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves
    Article Snippet: .. Upon confirmation of the recombinants with blue‐white colony screening and colony PCR, the easI gene was excised from the plasmid by digestion with FastDigest NcoI and BamHI (Thermo Scientific, USA), followed by ligation with linearized overexpression vector, pET28a (Novagen, Germany) to produce pET28a‐ easI . .. The DNA sequence of the constructed plasmids was verified by automated Sanger sequencing.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Thermo Fisher fastdigest ncoi
    Fastdigest Ncoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest ncoi/product/Thermo Fisher
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    fastdigest ncoi - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results