lc pcr master mix  (Roche)


Bioz Verified Symbol Roche is a verified supplier
Bioz Manufacturer Symbol Roche manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85

    Structured Review

    Roche lc pcr master mix
    Differences between the mean loads measured by the branched <t>DNA</t> assay and real-time <t>RT-PCR</t> by using the Bland-Altman plot analysis. The mean difference (bias) is indicated by a solid line, and the 95% confidence interval (±2 standard deviations)
    Lc Pcr Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 5811 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc pcr master mix/product/Roche
    Average 85 stars, based on 5811 article reviews
    Price from $9.99 to $1999.99
    lc pcr master mix - by Bioz Stars, 2020-09
    85/100 stars

    Images

    1) Product Images from "Real-Time PCR Quantification of Genital Shedding of Herpes Simplex Virus (HSV) and Human Immunodeficiency Virus (HIV) in Women Coinfected with HSV and HIV"

    Article Title: Real-Time PCR Quantification of Genital Shedding of Herpes Simplex Virus (HSV) and Human Immunodeficiency Virus (HIV) in Women Coinfected with HSV and HIV

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.44.2.423-432.2006

    Differences between the mean loads measured by the branched DNA assay and real-time RT-PCR by using the Bland-Altman plot analysis. The mean difference (bias) is indicated by a solid line, and the 95% confidence interval (±2 standard deviations)
    Figure Legend Snippet: Differences between the mean loads measured by the branched DNA assay and real-time RT-PCR by using the Bland-Altman plot analysis. The mean difference (bias) is indicated by a solid line, and the 95% confidence interval (±2 standard deviations)

    Techniques Used: Quantitative RT-PCR

    HSV-2 DNA quantification by real-time PCR.
    Figure Legend Snippet: HSV-2 DNA quantification by real-time PCR.

    Techniques Used: Real-time Polymerase Chain Reaction

    2) Product Images from "Aortic VCAM‐1: an early marker of vascular inflammation in collagen‐induced arthritis"

    Article Title: Aortic VCAM‐1: an early marker of vascular inflammation in collagen‐induced arthritis

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.12790

    Early (4 weeks) and late (15 weeks)  VCAM ‐1 staining in aortic sinus of mice. Aortic sinuses from  CIA  mice were removed at 4 and 15 weeks after immunization. Controls were immunized with only  CFA  or were  NI  mice. ( A ) Representative microphotographs of  VCAM ‐1 staining in aortic sinus from cCII/ CFA ‐immunized ( CIA : a, d),  CFA ‐immunized ( CFA : b, e) and  NI (c, f) mice. Black arrows show endothelial staining for  VCAM ‐1; white arrows show  VCAM ‐1 staining in the aortic sinus wall. ( B ) Quantification of  VCAM ‐1–staining at 4 and 15 weeks in  CIA  versus  control mice ( NI  and  CFA  mice pooled) by use of Image J Fiji.  £ P
    Figure Legend Snippet: Early (4 weeks) and late (15 weeks) VCAM ‐1 staining in aortic sinus of mice. Aortic sinuses from CIA mice were removed at 4 and 15 weeks after immunization. Controls were immunized with only CFA or were NI mice. ( A ) Representative microphotographs of VCAM ‐1 staining in aortic sinus from cCII/ CFA ‐immunized ( CIA : a, d), CFA ‐immunized ( CFA : b, e) and NI (c, f) mice. Black arrows show endothelial staining for VCAM ‐1; white arrows show VCAM ‐1 staining in the aortic sinus wall. ( B ) Quantification of VCAM ‐1–staining at 4 and 15 weeks in CIA versus control mice ( NI and CFA mice pooled) by use of Image J Fiji. £ P

    Techniques Used: Staining, Mouse Assay

    VCAM ‐1 staining in aortic sinus from mice fed an  HD  or standard chow diet. ( A ) Representative histological sections of the aortic sinus stained with blue‐haematoxylin for  VCAM ‐1 in  NI  mice fed a standard chow diet or an  HD  and in immunized mice fed a standard diet ( CIA ) or an  HD  ( CIA + HD ). Magnification is 50 × (a, d, g, j) or 400 × (b, e, h, k). Staining for isotype control of  VCAM ‐1 antibody (IgG2a, κ antibody) is shown in c, f, i and l (×400 magnification). Black arrows show  VCAM ‐1 endothelial staining; white arrows show  VCAM ‐1 staining in the aortic sinus wall. ( B ) Quantification of  VCAM ‐1–positive staining by use of Image J Fiji.  $ P
    Figure Legend Snippet: VCAM ‐1 staining in aortic sinus from mice fed an HD or standard chow diet. ( A ) Representative histological sections of the aortic sinus stained with blue‐haematoxylin for VCAM ‐1 in NI mice fed a standard chow diet or an HD and in immunized mice fed a standard diet ( CIA ) or an HD ( CIA + HD ). Magnification is 50 × (a, d, g, j) or 400 × (b, e, h, k). Staining for isotype control of VCAM ‐1 antibody (IgG2a, κ antibody) is shown in c, f, i and l (×400 magnification). Black arrows show VCAM ‐1 endothelial staining; white arrows show VCAM ‐1 staining in the aortic sinus wall. ( B ) Quantification of VCAM ‐1–positive staining by use of Image J Fiji. $ P

    Techniques Used: Staining, Mouse Assay

    Early (4 weeks) and late (15 weeks) aortic  mRNA  expression of  VCAM ‐1,  iNOS , and  IL ‐17 in  CIA  mice. Aortas from  CIA  mice were removed at 4 weeks ( n  = 12) and 15 weeks ( n  = 6) after immunization. Controls were: mice immunized with only  CFA  ( n  = 6) and nonimmunized mice ( NI ) ( n  = 12 or  n  = 6 for aorta removed at 4 or 15 weeks, respectively).  qRT ‐ PCR  analysis of  mRNA  levels of  VCAM ‐1 ( A ),  iNOS  ( B ) and  IL ‐17 ( C ). Data are mean ± S.E.M.;  $ P
    Figure Legend Snippet: Early (4 weeks) and late (15 weeks) aortic mRNA expression of VCAM ‐1, iNOS , and IL ‐17 in CIA mice. Aortas from CIA mice were removed at 4 weeks ( n = 12) and 15 weeks ( n = 6) after immunization. Controls were: mice immunized with only CFA ( n = 6) and nonimmunized mice ( NI ) ( n = 12 or n = 6 for aorta removed at 4 or 15 weeks, respectively). qRT ‐ PCR analysis of mRNA levels of VCAM ‐1 ( A ), iNOS ( B ) and IL ‐17 ( C ). Data are mean ± S.E.M.; $ P

    Techniques Used: Expressing, Mouse Assay, Quantitative RT-PCR

    mRNA  expression of pro‐inflammatory molecules in aortas ( A – C ) and synovium ( D – F ) of immunized and nonimmunized mice fed an  HD  or standard chow diet. Quantitative  RT ‐ PCR  ( qRT ‐ PCR ) analysis of  mRNA  levels of vascular cell adhesion molecule‐1 ( VCAM ‐1) ( A  and  D ), inducible nitric oxide synthase ( iNOS ) ( B  and  E ) and interleukin 17 ( IL ‐17) ( C  and  F ). Data are mean ± S.E.M.  £ P
    Figure Legend Snippet: mRNA expression of pro‐inflammatory molecules in aortas ( A – C ) and synovium ( D – F ) of immunized and nonimmunized mice fed an HD or standard chow diet. Quantitative RT ‐ PCR ( qRT ‐ PCR ) analysis of mRNA levels of vascular cell adhesion molecule‐1 ( VCAM ‐1) ( A and D ), inducible nitric oxide synthase ( iNOS ) ( B and E ) and interleukin 17 ( IL ‐17) ( C and F ). Data are mean ± S.E.M. £ P

    Techniques Used: Expressing, Mouse Assay, Quantitative RT-PCR

    3) Product Images from "Development of real-time PCR for detection of Mycoplasma hominis"

    Article Title: Development of real-time PCR for detection of Mycoplasma hominis

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-4-35

    Reproducibility of the LightCycler assay in clinical samples. The LightCycler PCR run of 10 negative clinical samples spiked with the known PG21 DNA concentration of 10 2 copies/μl. The assay showed no inhibition, all curves came up at the same time as 10 2 copies/μl of PG21 (marked with squares) from the standard dilution series.
    Figure Legend Snippet: Reproducibility of the LightCycler assay in clinical samples. The LightCycler PCR run of 10 negative clinical samples spiked with the known PG21 DNA concentration of 10 2 copies/μl. The assay showed no inhibition, all curves came up at the same time as 10 2 copies/μl of PG21 (marked with squares) from the standard dilution series.

    Techniques Used: Polymerase Chain Reaction, Concentration Assay, Inhibition

    Inhibition of the BEa and SP-4 media on LightCycler PCR. LightCycler PCR with SP-4 medium and BEa spiked with PG21 DNA of the concentrations: 10 5 , 10 4 , and 10 3 copies/μl, respectively.
    Figure Legend Snippet: Inhibition of the BEa and SP-4 media on LightCycler PCR. LightCycler PCR with SP-4 medium and BEa spiked with PG21 DNA of the concentrations: 10 5 , 10 4 , and 10 3 copies/μl, respectively.

    Techniques Used: Inhibition, Polymerase Chain Reaction

    DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.
    Figure Legend Snippet: DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.

    Techniques Used: Polymerase Chain Reaction

    Sensitivity of LightCycler PCR in standard dilution series. (a) The LightCycler PCR run with M. hominis PG21 DNA and fluorescence probes was done. The fluorescence signal of 10-fold dilution series from 10 5 to 5 and 1 copy is shown. No reaction was noted in the negative control (0 copies). (b) Standard curve was generated from the threshold cycles ( C t ) also known as crossing points ( Cp ) of the M. hominis PG21 standard dilution series by the LightCycler software.
    Figure Legend Snippet: Sensitivity of LightCycler PCR in standard dilution series. (a) The LightCycler PCR run with M. hominis PG21 DNA and fluorescence probes was done. The fluorescence signal of 10-fold dilution series from 10 5 to 5 and 1 copy is shown. No reaction was noted in the negative control (0 copies). (b) Standard curve was generated from the threshold cycles ( C t ) also known as crossing points ( Cp ) of the M. hominis PG21 standard dilution series by the LightCycler software.

    Techniques Used: Polymerase Chain Reaction, Fluorescence, Negative Control, Generated, Software

    Specificity of the LightCycler PCR. The LightCycler PCR run with different M. hominis isolates. Concentration of DNA from different isolates used in the study was estimated to 10 4 copies/μl of PG21 DNA, which was used as a standard DNA.
    Figure Legend Snippet: Specificity of the LightCycler PCR. The LightCycler PCR run with different M. hominis isolates. Concentration of DNA from different isolates used in the study was estimated to 10 4 copies/μl of PG21 DNA, which was used as a standard DNA.

    Techniques Used: Polymerase Chain Reaction, Concentration Assay

    Specificty of the LightCycler PCR. (a) LightCycler PCR with human DNA from Hep2 cells and selected Mycoplasma spp. run with the designed primers and probes. (b) Spiking assay showed no inhibition when DNA from five different Mycoplasma species ( M. arginini, M. bovis, M. hyorhinis, M. pulmonis, M. salivarium) was spiked with PG21 DNA of 10 2 copies/μl. All curves came up at the same time as 10 2 copies/μl of PG21 from the standard dilution series (marked with squares).
    Figure Legend Snippet: Specificty of the LightCycler PCR. (a) LightCycler PCR with human DNA from Hep2 cells and selected Mycoplasma spp. run with the designed primers and probes. (b) Spiking assay showed no inhibition when DNA from five different Mycoplasma species ( M. arginini, M. bovis, M. hyorhinis, M. pulmonis, M. salivarium) was spiked with PG21 DNA of 10 2 copies/μl. All curves came up at the same time as 10 2 copies/μl of PG21 from the standard dilution series (marked with squares).

    Techniques Used: Polymerase Chain Reaction, Inhibition

    4) Product Images from "Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis"

    Article Title: Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.40.6.2213-2216.2002

    Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.
    Figure Legend Snippet: Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.

    Techniques Used: Hybridization

    5) Product Images from "Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity"

    Article Title: Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity

    Journal: AIDS Research and Therapy

    doi: 10.1186/1742-6405-5-10

    Evaluation of C14 inhibitory activity on HIV-1 DNA content into primary cells. Monocyte-derived macrophages (A), monocyte-derived dendritic cells (B) and T cells (C) were incubated with R5 or X4 viruses in the presence of increasing concentrations of C14 or an unique dose of T 20 (5 μM) for 3 hours at 37°C. Cells were then washed and cultured in fresh medium for 3 days. DNA was extracted and the viral DNA was quantified by real time PCR. Means are representative of 3 independent experiments and assays were performed in triplicates. *, p ≤ 0.05; **, p ≤ 0.01 between untreated and treated cells.
    Figure Legend Snippet: Evaluation of C14 inhibitory activity on HIV-1 DNA content into primary cells. Monocyte-derived macrophages (A), monocyte-derived dendritic cells (B) and T cells (C) were incubated with R5 or X4 viruses in the presence of increasing concentrations of C14 or an unique dose of T 20 (5 μM) for 3 hours at 37°C. Cells were then washed and cultured in fresh medium for 3 days. DNA was extracted and the viral DNA was quantified by real time PCR. Means are representative of 3 independent experiments and assays were performed in triplicates. *, p ≤ 0.05; **, p ≤ 0.01 between untreated and treated cells.

    Techniques Used: Activity Assay, Derivative Assay, Incubation, Cell Culture, Real-time Polymerase Chain Reaction

    6) Product Images from "Challenging diagnosis of congenital malaria in non-endemic areas"

    Article Title: Challenging diagnosis of congenital malaria in non-endemic areas

    Journal: Malaria Journal

    doi: 10.1186/s12936-018-2614-9

    Electronic image of the gel displaying PCR product sizes of the six molecular markers amplified from mother and newborn DNA samples. The markers Pfmsp2 , FC27 (subfamily of Pfmsp2 ) and Pfhrp3 showed discordant genotypes between the two analyzed samples
    Figure Legend Snippet: Electronic image of the gel displaying PCR product sizes of the six molecular markers amplified from mother and newborn DNA samples. The markers Pfmsp2 , FC27 (subfamily of Pfmsp2 ) and Pfhrp3 showed discordant genotypes between the two analyzed samples

    Techniques Used: Polymerase Chain Reaction, Amplification

    Plasmodium spp. screening by 18S rRNA targeting PCR. M DNA marker, (1/2) Mother’s sample replicates; (3/4) male infant’s sample replicates; (5/6) female infant’s sample duplicates; (7/8) male infant’s sample duplicates
    Figure Legend Snippet: Plasmodium spp. screening by 18S rRNA targeting PCR. M DNA marker, (1/2) Mother’s sample replicates; (3/4) male infant’s sample replicates; (5/6) female infant’s sample duplicates; (7/8) male infant’s sample duplicates

    Techniques Used: Polymerase Chain Reaction, Marker

    Plasmodium falciparum typing by 18S rRNA targeting PCR. M DNA marker, (1) Mother’s sample; (2) male infant’s sample
    Figure Legend Snippet: Plasmodium falciparum typing by 18S rRNA targeting PCR. M DNA marker, (1) Mother’s sample; (2) male infant’s sample

    Techniques Used: Polymerase Chain Reaction, Marker

    7) Product Images from "SMA Diagnosis: Detection of SMN1 Deletion with Real-Time mCOP-PCR System Using Fresh Blood DNA"

    Article Title: SMA Diagnosis: Detection of SMN1 Deletion with Real-Time mCOP-PCR System Using Fresh Blood DNA

    Journal: Kobe Journal of Medical Sciences

    doi:

    Selective amplification of SMN1 and SMN2 by real-time mCOP-PCR. Gene-specific amplification of SMN1 / SMN2 exon 7 by real-time mCOP-PCR. (A) Amplification curves of SMN1 for the three different groups. The numbers shown next to each curve represents the sample number in each group. The samples with SMN1 (+) showed significant amplification, while the samples with SMN1 (−) showed no significant amplification. (B) Amplification curves of SMN2 for the three different groups. The numbers shown next to each curve represents the sample number in each group. The samples with SMN2 (+) showed significant amplification, while the samples with SMN2 (−) showed no significant amplification.
    Figure Legend Snippet: Selective amplification of SMN1 and SMN2 by real-time mCOP-PCR. Gene-specific amplification of SMN1 / SMN2 exon 7 by real-time mCOP-PCR. (A) Amplification curves of SMN1 for the three different groups. The numbers shown next to each curve represents the sample number in each group. The samples with SMN1 (+) showed significant amplification, while the samples with SMN1 (−) showed no significant amplification. (B) Amplification curves of SMN2 for the three different groups. The numbers shown next to each curve represents the sample number in each group. The samples with SMN2 (+) showed significant amplification, while the samples with SMN2 (−) showed no significant amplification.

    Techniques Used: Amplification, Polymerase Chain Reaction

    8) Product Images from "Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis"

    Article Title: Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.40.6.2213-2216.2002

    Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.
    Figure Legend Snippet: Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.

    Techniques Used: Hybridization

    (A) Melting curves for the LightCycler QJ3 BDE and QJ4 BDE FRET hybridization probes. The specific melting peak can be observed only for the strains with the ptxS1B , ptxS1D , and ptxS1E alleles. (B) Melting curves for the LightCycler QJ5 E and QJ6 E FRET hybridization probes, demonstrating differentiation of the strains that harbor the ptxS1E allele from the vaccine type strains ( ptxS1B and ptxS1D ). A specific melting peak can be observed only for the strains with the ptxS1E allele.
    Figure Legend Snippet: (A) Melting curves for the LightCycler QJ3 BDE and QJ4 BDE FRET hybridization probes. The specific melting peak can be observed only for the strains with the ptxS1B , ptxS1D , and ptxS1E alleles. (B) Melting curves for the LightCycler QJ5 E and QJ6 E FRET hybridization probes, demonstrating differentiation of the strains that harbor the ptxS1E allele from the vaccine type strains ( ptxS1B and ptxS1D ). A specific melting peak can be observed only for the strains with the ptxS1E allele.

    Techniques Used: Hybridization

    9) Product Images from "Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis"

    Article Title: Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.40.6.2213-2216.2002

    Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.
    Figure Legend Snippet: Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.

    Techniques Used: Hybridization

    (A) Melting curves for the LightCycler QJ3 BDE and QJ4 BDE FRET hybridization probes. The specific melting peak can be observed only for the strains with the ptxS1B , ptxS1D , and ptxS1E alleles. (B) Melting curves for the LightCycler QJ5 E and QJ6 E FRET hybridization probes, demonstrating differentiation of the strains that harbor the ptxS1E allele from the vaccine type strains ( ptxS1B and ptxS1D ). A specific melting peak can be observed only for the strains with the ptxS1E allele.
    Figure Legend Snippet: (A) Melting curves for the LightCycler QJ3 BDE and QJ4 BDE FRET hybridization probes. The specific melting peak can be observed only for the strains with the ptxS1B , ptxS1D , and ptxS1E alleles. (B) Melting curves for the LightCycler QJ5 E and QJ6 E FRET hybridization probes, demonstrating differentiation of the strains that harbor the ptxS1E allele from the vaccine type strains ( ptxS1B and ptxS1D ). A specific melting peak can be observed only for the strains with the ptxS1E allele.

    Techniques Used: Hybridization

    10) Product Images from "Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis"

    Article Title: Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.40.6.2213-2216.2002

    Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.
    Figure Legend Snippet: Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.

    Techniques Used: Hybridization

    (A) Melting curves for the LightCycler QJ3 BDE and QJ4 BDE FRET hybridization probes. The specific melting peak can be observed only for the strains with the ptxS1B , ptxS1D , and ptxS1E alleles. (B) Melting curves for the LightCycler QJ5 E and QJ6 E FRET hybridization probes, demonstrating differentiation of the strains that harbor the ptxS1E allele from the vaccine type strains ( ptxS1B and ptxS1D ). A specific melting peak can be observed only for the strains with the ptxS1E allele.
    Figure Legend Snippet: (A) Melting curves for the LightCycler QJ3 BDE and QJ4 BDE FRET hybridization probes. The specific melting peak can be observed only for the strains with the ptxS1B , ptxS1D , and ptxS1E alleles. (B) Melting curves for the LightCycler QJ5 E and QJ6 E FRET hybridization probes, demonstrating differentiation of the strains that harbor the ptxS1E allele from the vaccine type strains ( ptxS1B and ptxS1D ). A specific melting peak can be observed only for the strains with the ptxS1E allele.

    Techniques Used: Hybridization

    11) Product Images from "Real-Time PCR Quantification of Genital Shedding of Herpes Simplex Virus (HSV) and Human Immunodeficiency Virus (HIV) in Women Coinfected with HSV and HIV"

    Article Title: Real-Time PCR Quantification of Genital Shedding of Herpes Simplex Virus (HSV) and Human Immunodeficiency Virus (HIV) in Women Coinfected with HSV and HIV

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.44.2.423-432.2006

    Genital HIV-1 RNA shedding in HSV-2 DNA-shedding and -nonshedding women coinfected with HSV-2 and HIV-1. Genital HIV-1 RNA loads from HSV-2 DNA-shedding and HSV-2-nonshedding women, expressed in log 10 copies/ml, are represented by triangles. Means of
    Figure Legend Snippet: Genital HIV-1 RNA shedding in HSV-2 DNA-shedding and -nonshedding women coinfected with HSV-2 and HIV-1. Genital HIV-1 RNA loads from HSV-2 DNA-shedding and HSV-2-nonshedding women, expressed in log 10 copies/ml, are represented by triangles. Means of

    Techniques Used:

    12) Product Images from "Protein and mRNA expression of uPAR and PAI-1 in myoepithelial cells of early breast cancer lesions and normal breast tissue"

    Article Title: Protein and mRNA expression of uPAR and PAI-1 in myoepithelial cells of early breast cancer lesions and normal breast tissue

    Journal: British Journal of Cancer

    doi: 10.1038/sj.bjc.6601990

    ( A ) Quantitative RT–PCR of mRNA derived from microdissected MEs of normal breast tissue, low- and high-grade DCIS using PAI-1-specific primers. From each group one case was selected and a LightCycler™ analysis was performed in triplicate. The amount of PAI-1 mRNA was calculated by assorting each CP to a standard curve. ( B ) The RNA of MEs (DCIS and normal breast tissue) was isolated, reverse transcriptase reaction followed by a PCR using uPAR primers (see Materials and methods) was performed. The RT–PCR reveals a 311 bp product in both probes (DCIS and normal breast tissue); without RT reaction no product was received.
    Figure Legend Snippet: ( A ) Quantitative RT–PCR of mRNA derived from microdissected MEs of normal breast tissue, low- and high-grade DCIS using PAI-1-specific primers. From each group one case was selected and a LightCycler™ analysis was performed in triplicate. The amount of PAI-1 mRNA was calculated by assorting each CP to a standard curve. ( B ) The RNA of MEs (DCIS and normal breast tissue) was isolated, reverse transcriptase reaction followed by a PCR using uPAR primers (see Materials and methods) was performed. The RT–PCR reveals a 311 bp product in both probes (DCIS and normal breast tissue); without RT reaction no product was received.

    Techniques Used: Quantitative RT-PCR, Derivative Assay, Isolation, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

    13) Product Images from "Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity"

    Article Title: Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity

    Journal: AIDS Research and Therapy

    doi: 10.1186/1742-6405-5-10

    Evaluation of C14 inhibitory activity on HIV-1 DNA content into primary cells. Monocyte-derived macrophages (A), monocyte-derived dendritic cells (B) and T cells (C) were incubated with R5 or X4 viruses in the presence of increasing concentrations of C14 or an unique dose of T 20 (5 μM) for 3 hours at 37°C. Cells were then washed and cultured in fresh medium for 3 days. DNA was extracted and the viral DNA was quantified by real time PCR. Means are representative of 3 independent experiments and assays were performed in triplicates. *, p ≤ 0.05; **, p ≤ 0.01 between untreated and treated cells.
    Figure Legend Snippet: Evaluation of C14 inhibitory activity on HIV-1 DNA content into primary cells. Monocyte-derived macrophages (A), monocyte-derived dendritic cells (B) and T cells (C) were incubated with R5 or X4 viruses in the presence of increasing concentrations of C14 or an unique dose of T 20 (5 μM) for 3 hours at 37°C. Cells were then washed and cultured in fresh medium for 3 days. DNA was extracted and the viral DNA was quantified by real time PCR. Means are representative of 3 independent experiments and assays were performed in triplicates. *, p ≤ 0.05; **, p ≤ 0.01 between untreated and treated cells.

    Techniques Used: Activity Assay, Derivative Assay, Incubation, Cell Culture, Real-time Polymerase Chain Reaction

    14) Product Images from "Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis"

    Article Title: Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.40.6.2213-2216.2002

    Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.
    Figure Legend Snippet: Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.

    Techniques Used: Hybridization

    (A) Melting curves for the LightCycler QJ3 BDE and QJ4 BDE FRET hybridization probes. The specific melting peak can be observed only for the strains with the ptxS1B , ptxS1D , and ptxS1E alleles. (B) Melting curves for the LightCycler QJ5 E and QJ6 E FRET hybridization probes, demonstrating differentiation of the strains that harbor the ptxS1E allele from the vaccine type strains ( ptxS1B and ptxS1D ). A specific melting peak can be observed only for the strains with the ptxS1E allele.
    Figure Legend Snippet: (A) Melting curves for the LightCycler QJ3 BDE and QJ4 BDE FRET hybridization probes. The specific melting peak can be observed only for the strains with the ptxS1B , ptxS1D , and ptxS1E alleles. (B) Melting curves for the LightCycler QJ5 E and QJ6 E FRET hybridization probes, demonstrating differentiation of the strains that harbor the ptxS1E allele from the vaccine type strains ( ptxS1B and ptxS1D ). A specific melting peak can be observed only for the strains with the ptxS1E allele.

    Techniques Used: Hybridization

    15) Product Images from "Challenging diagnosis of congenital malaria in non-endemic areas"

    Article Title: Challenging diagnosis of congenital malaria in non-endemic areas

    Journal: Malaria Journal

    doi: 10.1186/s12936-018-2614-9

    Plasmodium spp. screening by 18S rRNA targeting PCR. M DNA marker, (1/2) Mother’s sample replicates; (3/4) male infant’s sample replicates; (5/6) female infant’s sample duplicates; (7/8) male infant’s sample duplicates
    Figure Legend Snippet: Plasmodium spp. screening by 18S rRNA targeting PCR. M DNA marker, (1/2) Mother’s sample replicates; (3/4) male infant’s sample replicates; (5/6) female infant’s sample duplicates; (7/8) male infant’s sample duplicates

    Techniques Used: Polymerase Chain Reaction, Marker

    Plasmodium falciparum typing by 18S rRNA targeting PCR. M DNA marker, (1) Mother’s sample; (2) male infant’s sample
    Figure Legend Snippet: Plasmodium falciparum typing by 18S rRNA targeting PCR. M DNA marker, (1) Mother’s sample; (2) male infant’s sample

    Techniques Used: Polymerase Chain Reaction, Marker

    16) Product Images from "Treponema denticola Does Not Induce Production of Common Innate Immune Mediators from Primary Gingival Epithelial Cells"

    Article Title: Treponema denticola Does Not Induce Production of Common Innate Immune Mediators from Primary Gingival Epithelial Cells

    Journal:

    doi: 10.1111/j.1399-302X.2008.00452.x

    T. denticola does not induce IL-8 or hβD-2 transcription in PGEC
    Figure Legend Snippet: T. denticola does not induce IL-8 or hβD-2 transcription in PGEC

    Techniques Used:

    17) Product Images from "Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis"

    Article Title: Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.40.6.2213-2216.2002

    Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.
    Figure Legend Snippet: Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.

    Techniques Used: Hybridization

    (A) Melting curves for the LightCycler QJ3 BDE and QJ4 BDE FRET hybridization probes. The specific melting peak can be observed only for the strains with the ptxS1B , ptxS1D , and ptxS1E alleles. (B) Melting curves for the LightCycler QJ5 E and QJ6 E FRET hybridization probes, demonstrating differentiation of the strains that harbor the ptxS1E allele from the vaccine type strains ( ptxS1B and ptxS1D ). A specific melting peak can be observed only for the strains with the ptxS1E allele.
    Figure Legend Snippet: (A) Melting curves for the LightCycler QJ3 BDE and QJ4 BDE FRET hybridization probes. The specific melting peak can be observed only for the strains with the ptxS1B , ptxS1D , and ptxS1E alleles. (B) Melting curves for the LightCycler QJ5 E and QJ6 E FRET hybridization probes, demonstrating differentiation of the strains that harbor the ptxS1E allele from the vaccine type strains ( ptxS1B and ptxS1D ). A specific melting peak can be observed only for the strains with the ptxS1E allele.

    Techniques Used: Hybridization

    18) Product Images from "Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis"

    Article Title: Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.40.6.2213-2216.2002

    Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.
    Figure Legend Snippet: Polymorphic nucleotide sequences of the ptxS1 subunit. For ptxS1A, ptxS1B , and ptxS1E , only differences from ptxS1D are indicated. Dots indicate identical bases. Numbers refer to positions in the ptxS1D allele. The nucleotide differences that are used as targets for hybridization probes are boldfaced.

    Techniques Used: Hybridization

    (A) Melting curves for the LightCycler QJ3 BDE and QJ4 BDE FRET hybridization probes. The specific melting peak can be observed only for the strains with the ptxS1B , ptxS1D , and ptxS1E alleles. (B) Melting curves for the LightCycler QJ5 E and QJ6 E FRET hybridization probes, demonstrating differentiation of the strains that harbor the ptxS1E allele from the vaccine type strains ( ptxS1B and ptxS1D ). A specific melting peak can be observed only for the strains with the ptxS1E allele.
    Figure Legend Snippet: (A) Melting curves for the LightCycler QJ3 BDE and QJ4 BDE FRET hybridization probes. The specific melting peak can be observed only for the strains with the ptxS1B , ptxS1D , and ptxS1E alleles. (B) Melting curves for the LightCycler QJ5 E and QJ6 E FRET hybridization probes, demonstrating differentiation of the strains that harbor the ptxS1E allele from the vaccine type strains ( ptxS1B and ptxS1D ). A specific melting peak can be observed only for the strains with the ptxS1E allele.

    Techniques Used: Hybridization

    19) Product Images from "Polymerase chain reaction and deoxyribonucleic acid-sequencing based study on distribution of human papillomavirus 16/18 among histopathological types of cervical intra-epithelial neoplasia and primary invasive cervical carcinoma: A scenario in North Bengal, India"

    Article Title: Polymerase chain reaction and deoxyribonucleic acid-sequencing based study on distribution of human papillomavirus 16/18 among histopathological types of cervical intra-epithelial neoplasia and primary invasive cervical carcinoma: A scenario in North Bengal, India

    Journal: Journal of Mid-Life Health

    doi: 10.4103/0976-7800.127786

    L1 (My09/11) polymerase chain reaction products. Lane 1: 50 bp deoxyribonucleic acid ladder; lanes 2-7: Samples showing specific band for L1 at 450 bp; lane 8: L1 (–)ve sample; lane 9: Positive control; lane 10: Negative control
    Figure Legend Snippet: L1 (My09/11) polymerase chain reaction products. Lane 1: 50 bp deoxyribonucleic acid ladder; lanes 2-7: Samples showing specific band for L1 at 450 bp; lane 8: L1 (–)ve sample; lane 9: Positive control; lane 10: Negative control

    Techniques Used: Polymerase Chain Reaction, Positive Control, Negative Control

    Representative electropherograms showing aligned sequences of L1 obtained by polymerase chain reaction with MY09/11 primers
    Figure Legend Snippet: Representative electropherograms showing aligned sequences of L1 obtained by polymerase chain reaction with MY09/11 primers

    Techniques Used: Polymerase Chain Reaction

    20) Product Images from "Treponema denticola Does Not Induce Production of Common Innate Immune Mediators from Primary Gingival Epithelial Cells"

    Article Title: Treponema denticola Does Not Induce Production of Common Innate Immune Mediators from Primary Gingival Epithelial Cells

    Journal:

    doi: 10.1111/j.1399-302X.2008.00452.x

    T. denticola does not induce IL-8 protein from HUVEC
    Figure Legend Snippet: T. denticola does not induce IL-8 protein from HUVEC

    Techniques Used:

    T. denticola 35405 dentilisin mutant does not induce production of IL-8 protein
    Figure Legend Snippet: T. denticola 35405 dentilisin mutant does not induce production of IL-8 protein

    Techniques Used: Mutagenesis

    T. denticola dentilisin mutant, K1, does not degrade IL-8 protein
    Figure Legend Snippet: T. denticola dentilisin mutant, K1, does not degrade IL-8 protein

    Techniques Used: Mutagenesis

    T. denticola does not induce IL-8 or hβD-2 transcription in PGEC
    Figure Legend Snippet: T. denticola does not induce IL-8 or hβD-2 transcription in PGEC

    Techniques Used:

    T. denticola does not induce production of IL-8 protein from PGEC
    Figure Legend Snippet: T. denticola does not induce production of IL-8 protein from PGEC

    Techniques Used:

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Src Subfamily Kinases Regulate Nuclear Export and Degradation of Transcription Factor Nrf2 to Switch Off Nrf2-mediated Antioxidant Activation of Cytoprotective Gene Expression *
    Article Snippet: .. Similarly, SYF+/+ and SYF−/− mouse embryonic fibroblast cells were plated in 96-well plates, treated with different concentrations of etoposide (1–16 μ m ) for 48 h, and post-treated with DMSO or t -BHQ for an additional 24 h. Cells were harvested, and a photometric enzyme immunoassay was performed for the quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after etoposide exposure to cells using Cell Death Detection ELISA kit (Roche Applied Science) per the manufacturer's instructions. ..

    In Vitro:

    Article Title: Src Subfamily Kinases Regulate Nuclear Export and Degradation of Transcription Factor Nrf2 to Switch Off Nrf2-mediated Antioxidant Activation of Cytoprotective Gene Expression *
    Article Snippet: .. Similarly, SYF+/+ and SYF−/− mouse embryonic fibroblast cells were plated in 96-well plates, treated with different concentrations of etoposide (1–16 μ m ) for 48 h, and post-treated with DMSO or t -BHQ for an additional 24 h. Cells were harvested, and a photometric enzyme immunoassay was performed for the quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after etoposide exposure to cells using Cell Death Detection ELISA kit (Roche Applied Science) per the manufacturer's instructions. ..

    Isolation:

    Article Title: Pleiotropic Anti-Angiogenic and Anti-Oncogenic Activities of the Novel Mithralog Demycarosyl-3D-ß-D-Digitoxosyl-Mithramycin SK (EC-8042)
    Article Snippet: .. Quantitative Real Time PCR (qRT-PCR) Total RNA was isolated from cells treated with DMSO, MTA (200 nM), or DIG-MSK (200 nM) for 8 hours using the High Pure RNA Isolating kit (Roche). cDNA was obtained using High Capacity cDNA Reverse Transcription Kit (Invitrogen). .. Quantitative Real Time PCR (qRT-PCR) was performed in triplicate by using HT7900 RT-PCR and SYBR Green PCR Master Mix (Applied Biosystems).

    Labeling:

    Article Title: Enrichment Followed by Quantitative PCR both for Rapid Detection and as a Tool for Quantitative Risk Assessment of Food-Borne Thermotolerant Campylobacters
    Article Snippet: .. The 25-μl real-time PCR mixture contained 1× PCR buffer for Tth DNA polymerase (Roche A/S, Hvidovre, Denmark), 1 U of Tth DNA polymerase (Roche A/S), 0.4 mM deoxynucleoside triphosphate mixture (Amersham Pharmacia Biotech, Buckinghamshire, United Kingdom), 0.44 μM forward primer 5′ CTG CTT AAC ACA AGT TGA GTA GG 3′, 0.48 μM reverse primer 5′ TTC CTT AGG TAC CGT CAG AA 3′ (DNA Technology, Århus, Denmark; C. jejuni 16S rRNA; GenBank accession no. ), 2.5 mM MgCl2 (Applied Biosystems), 30 μg of bovine serum albumin (BSA) for chicken samples and 5 μg of BSA for pure DNA (Roche A/S), 20 nM target Campylobacter probe labeled with 6-carboxyfluorescein (FAM; reporter dye) and 6-carboxytetramethylrhodamine (TAMRA; quencher dye) (5′ FAM-TGT CAT CCT CCA CGC GGC GTT GCT GC-TAMRA 3′; DNA Technology), 50 nM IAC probe (5′ VIC-TTC ATG AGG ACA CCT GAG TTG A-TAMRA 3′; Applied Biosystems), 5 × 103 copies of IAC (124 bp), and 5 μl of DNA sample. .. The cycle profile was as follows: initial denaturation at 95°C for 3 min, followed by 40 cycles of 95°C for 15 s and 58°C for 60 s. Fluorescence measurements were obtained online and analyzed on the ABI-PRISM with the SDS software (version 1.7a; Applied Biosystems) and on the RotorGene with the version 4.6 software (Corbett Research).

    Real-time Polymerase Chain Reaction:

    Article Title: Enrichment Followed by Quantitative PCR both for Rapid Detection and as a Tool for Quantitative Risk Assessment of Food-Borne Thermotolerant Campylobacters
    Article Snippet: .. The 25-μl real-time PCR mixture contained 1× PCR buffer for Tth DNA polymerase (Roche A/S, Hvidovre, Denmark), 1 U of Tth DNA polymerase (Roche A/S), 0.4 mM deoxynucleoside triphosphate mixture (Amersham Pharmacia Biotech, Buckinghamshire, United Kingdom), 0.44 μM forward primer 5′ CTG CTT AAC ACA AGT TGA GTA GG 3′, 0.48 μM reverse primer 5′ TTC CTT AGG TAC CGT CAG AA 3′ (DNA Technology, Århus, Denmark; C. jejuni 16S rRNA; GenBank accession no. ), 2.5 mM MgCl2 (Applied Biosystems), 30 μg of bovine serum albumin (BSA) for chicken samples and 5 μg of BSA for pure DNA (Roche A/S), 20 nM target Campylobacter probe labeled with 6-carboxyfluorescein (FAM; reporter dye) and 6-carboxytetramethylrhodamine (TAMRA; quencher dye) (5′ FAM-TGT CAT CCT CCA CGC GGC GTT GCT GC-TAMRA 3′; DNA Technology), 50 nM IAC probe (5′ VIC-TTC ATG AGG ACA CCT GAG TTG A-TAMRA 3′; Applied Biosystems), 5 × 103 copies of IAC (124 bp), and 5 μl of DNA sample. .. The cycle profile was as follows: initial denaturation at 95°C for 3 min, followed by 40 cycles of 95°C for 15 s and 58°C for 60 s. Fluorescence measurements were obtained online and analyzed on the ABI-PRISM with the SDS software (version 1.7a; Applied Biosystems) and on the RotorGene with the version 4.6 software (Corbett Research).

    Article Title: Pleiotropic Anti-Angiogenic and Anti-Oncogenic Activities of the Novel Mithralog Demycarosyl-3D-ß-D-Digitoxosyl-Mithramycin SK (EC-8042)
    Article Snippet: .. Quantitative Real Time PCR (qRT-PCR) Total RNA was isolated from cells treated with DMSO, MTA (200 nM), or DIG-MSK (200 nM) for 8 hours using the High Pure RNA Isolating kit (Roche). cDNA was obtained using High Capacity cDNA Reverse Transcription Kit (Invitrogen). .. Quantitative Real Time PCR (qRT-PCR) was performed in triplicate by using HT7900 RT-PCR and SYBR Green PCR Master Mix (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: Real-Time PCR Quantification of Genital Shedding of Herpes Simplex Virus (HSV) and Human Immunodeficiency Virus (HIV) in Women Coinfected with HSV and HIV
    Article Snippet: .. The LC PCR master mix contained 1× Fast-Start Taq DNA polymerase reaction buffer (Roche Diagnostics), 3 mM MgCl2 , 0.5 μM concentrations of each primer, 0.2 μM HSV-2 FLU, and 0.4 μM HSV-2 LCR. .. Cycling conditions were as follows: initial denaturation and Fast-Start Taq DNA polymerase activation at 95°C for 10 min, 45 cycles of denaturation at 95°C for 5 s, annealing at 58°C for 10 s (with fluorescence acquisition at the end of each annealing stage), and extension at 72°C for 12 s. After amplification was complete, melting curve analysis was performed as follows: denaturation at 95°C for 20 s and annealing at 45°C for 30 s followed by a gradual increase in temperature (transition rate of 0.1°C/s) to 85°C with continuous fluorescence acquisition.

    Article Title: Enrichment Followed by Quantitative PCR both for Rapid Detection and as a Tool for Quantitative Risk Assessment of Food-Borne Thermotolerant Campylobacters
    Article Snippet: .. The 25-μl real-time PCR mixture contained 1× PCR buffer for Tth DNA polymerase (Roche A/S, Hvidovre, Denmark), 1 U of Tth DNA polymerase (Roche A/S), 0.4 mM deoxynucleoside triphosphate mixture (Amersham Pharmacia Biotech, Buckinghamshire, United Kingdom), 0.44 μM forward primer 5′ CTG CTT AAC ACA AGT TGA GTA GG 3′, 0.48 μM reverse primer 5′ TTC CTT AGG TAC CGT CAG AA 3′ (DNA Technology, Århus, Denmark; C. jejuni 16S rRNA; GenBank accession no. ), 2.5 mM MgCl2 (Applied Biosystems), 30 μg of bovine serum albumin (BSA) for chicken samples and 5 μg of BSA for pure DNA (Roche A/S), 20 nM target Campylobacter probe labeled with 6-carboxyfluorescein (FAM; reporter dye) and 6-carboxytetramethylrhodamine (TAMRA; quencher dye) (5′ FAM-TGT CAT CCT CCA CGC GGC GTT GCT GC-TAMRA 3′; DNA Technology), 50 nM IAC probe (5′ VIC-TTC ATG AGG ACA CCT GAG TTG A-TAMRA 3′; Applied Biosystems), 5 × 103 copies of IAC (124 bp), and 5 μl of DNA sample. .. The cycle profile was as follows: initial denaturation at 95°C for 3 min, followed by 40 cycles of 95°C for 15 s and 58°C for 60 s. Fluorescence measurements were obtained online and analyzed on the ABI-PRISM with the SDS software (version 1.7a; Applied Biosystems) and on the RotorGene with the version 4.6 software (Corbett Research).

    Article Title: Preferential MGMT methylation could predispose a subset of KIT/PDGFRA-WT GISTs, including SDH-deficient ones, to respond to alkylating agents
    Article Snippet: .. PCR was carried out in a total volume of 25 μl consisting in 20 ng of DNA, 10 × PCR buffer, MgCl2, dNTP, primers (10 pM each), and 1 U FastStart DNA Taq polymerase (Roche). .. PCR conditions were an initial denaturation of 95 °C for 5 min, followed by 40 cycles of 95 °C for 30 s, 52–64 °C for 30 s, and 72 °C for 30 s. PCR products were purified with the Qiaquick PCR purification kit (Qiagen) and sequenced on both strands using the Big Dye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems).

    Incubation:

    Article Title: A novel Aurora-A kinase inhibitor MLN8237 induces cytotoxicity and cell-cycle arrest in multiple myeloma
    Article Snippet: .. MM cells were exposed to DMSO or 0.5 to 1μM of MLN8237 for 24 to 72 hours, permeabilized by 70% ethanol at −20°C, and incubated with 50 μg/mL PI and 20 units/mL RNase-A (Roche Diagnostics). .. DNA content was analyzed by flow cytometry using BDFACS-Canto II (BD Biosciences) and FlowJo software.

    Article Title: The Retinoid X Receptor Agonist, 9-cis UAB30, Inhibits Cutaneous T-cell Lymphoma Proliferation Through the SKP2-p27kip1 Axis
    Article Snippet: .. CTCL cells, plated as above, were incubated with UAB30 or bexarotene or with DMSO for 24 h. Cells were lysed with RIPA buffer containing protease inhibitors (Roche, Indianapolis, IN), centrifuged, and the protein concentrations in the supernatants were determined by the bicinconinic acid (BCA) method (Thermo Fisher Scientific Inc.). .. Equal amounts of protein lysates were separated by 8% or 10% SDS-polyacrylamide gel electrophoresis, transferred onto PVDF membranes, and probed with primary antibodies followed by the appropriate secondary antibodies coupled to horseradish peroxidase.

    Quantitative RT-PCR:

    Article Title: Pleiotropic Anti-Angiogenic and Anti-Oncogenic Activities of the Novel Mithralog Demycarosyl-3D-ß-D-Digitoxosyl-Mithramycin SK (EC-8042)
    Article Snippet: .. Quantitative Real Time PCR (qRT-PCR) Total RNA was isolated from cells treated with DMSO, MTA (200 nM), or DIG-MSK (200 nM) for 8 hours using the High Pure RNA Isolating kit (Roche). cDNA was obtained using High Capacity cDNA Reverse Transcription Kit (Invitrogen). .. Quantitative Real Time PCR (qRT-PCR) was performed in triplicate by using HT7900 RT-PCR and SYBR Green PCR Master Mix (Applied Biosystems).

    BIA-KA:

    Article Title: The Retinoid X Receptor Agonist, 9-cis UAB30, Inhibits Cutaneous T-cell Lymphoma Proliferation Through the SKP2-p27kip1 Axis
    Article Snippet: .. CTCL cells, plated as above, were incubated with UAB30 or bexarotene or with DMSO for 24 h. Cells were lysed with RIPA buffer containing protease inhibitors (Roche, Indianapolis, IN), centrifuged, and the protein concentrations in the supernatants were determined by the bicinconinic acid (BCA) method (Thermo Fisher Scientific Inc.). .. Equal amounts of protein lysates were separated by 8% or 10% SDS-polyacrylamide gel electrophoresis, transferred onto PVDF membranes, and probed with primary antibodies followed by the appropriate secondary antibodies coupled to horseradish peroxidase.

    CTG Assay:

    Article Title: Enrichment Followed by Quantitative PCR both for Rapid Detection and as a Tool for Quantitative Risk Assessment of Food-Borne Thermotolerant Campylobacters
    Article Snippet: .. The 25-μl real-time PCR mixture contained 1× PCR buffer for Tth DNA polymerase (Roche A/S, Hvidovre, Denmark), 1 U of Tth DNA polymerase (Roche A/S), 0.4 mM deoxynucleoside triphosphate mixture (Amersham Pharmacia Biotech, Buckinghamshire, United Kingdom), 0.44 μM forward primer 5′ CTG CTT AAC ACA AGT TGA GTA GG 3′, 0.48 μM reverse primer 5′ TTC CTT AGG TAC CGT CAG AA 3′ (DNA Technology, Århus, Denmark; C. jejuni 16S rRNA; GenBank accession no. ), 2.5 mM MgCl2 (Applied Biosystems), 30 μg of bovine serum albumin (BSA) for chicken samples and 5 μg of BSA for pure DNA (Roche A/S), 20 nM target Campylobacter probe labeled with 6-carboxyfluorescein (FAM; reporter dye) and 6-carboxytetramethylrhodamine (TAMRA; quencher dye) (5′ FAM-TGT CAT CCT CCA CGC GGC GTT GCT GC-TAMRA 3′; DNA Technology), 50 nM IAC probe (5′ VIC-TTC ATG AGG ACA CCT GAG TTG A-TAMRA 3′; Applied Biosystems), 5 × 103 copies of IAC (124 bp), and 5 μl of DNA sample. .. The cycle profile was as follows: initial denaturation at 95°C for 3 min, followed by 40 cycles of 95°C for 15 s and 58°C for 60 s. Fluorescence measurements were obtained online and analyzed on the ABI-PRISM with the SDS software (version 1.7a; Applied Biosystems) and on the RotorGene with the version 4.6 software (Corbett Research).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Enrichment Followed by Quantitative PCR both for Rapid Detection and as a Tool for Quantitative Risk Assessment of Food-Borne Thermotolerant Campylobacters
    Article Snippet: .. The 25-μl real-time PCR mixture contained 1× PCR buffer for Tth DNA polymerase (Roche A/S, Hvidovre, Denmark), 1 U of Tth DNA polymerase (Roche A/S), 0.4 mM deoxynucleoside triphosphate mixture (Amersham Pharmacia Biotech, Buckinghamshire, United Kingdom), 0.44 μM forward primer 5′ CTG CTT AAC ACA AGT TGA GTA GG 3′, 0.48 μM reverse primer 5′ TTC CTT AGG TAC CGT CAG AA 3′ (DNA Technology, Århus, Denmark; C. jejuni 16S rRNA; GenBank accession no. ), 2.5 mM MgCl2 (Applied Biosystems), 30 μg of bovine serum albumin (BSA) for chicken samples and 5 μg of BSA for pure DNA (Roche A/S), 20 nM target Campylobacter probe labeled with 6-carboxyfluorescein (FAM; reporter dye) and 6-carboxytetramethylrhodamine (TAMRA; quencher dye) (5′ FAM-TGT CAT CCT CCA CGC GGC GTT GCT GC-TAMRA 3′; DNA Technology), 50 nM IAC probe (5′ VIC-TTC ATG AGG ACA CCT GAG TTG A-TAMRA 3′; Applied Biosystems), 5 × 103 copies of IAC (124 bp), and 5 μl of DNA sample. .. The cycle profile was as follows: initial denaturation at 95°C for 3 min, followed by 40 cycles of 95°C for 15 s and 58°C for 60 s. Fluorescence measurements were obtained online and analyzed on the ABI-PRISM with the SDS software (version 1.7a; Applied Biosystems) and on the RotorGene with the version 4.6 software (Corbett Research).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Roche fast start taq polymerase buffer
    Fast Start Taq Polymerase Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast start taq polymerase buffer/product/Roche
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    fast start taq polymerase buffer - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results