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Abcam fascin ab74487 abcam
Fascin Ab74487 Abcam, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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fascin ab74487 abcam - by Bioz Stars, 2020-07
86/100 stars

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Incubation:

Article Title: The cognitive defects of neonatally irradiated mice are accompanied by changed synaptic plasticity, adult neurogenesis and neuroinflammation
Article Snippet: .. Immunoblotting analysis Hippocampal and cortical protein extracts (15 μg) were separated on 12% SDS polyacrylamide gels and were blotted to nitrocellulose membranes (GE Healthcare, Germany) via BIO-RAD criterion™ Blotter system at 100 V for 2 h. Membranes were blocked with RotiR -Block solution (Roth, Germany), washed and incubated overnight at 4°C with primary antibody dilutions as indicated by the manufacturer (GAPDH – sc-47724 Santa Cruz, Germany; CDC42 – ab106374 Abcam, Germany; Rac1 – ab33186 Abcam, Germany; p-PAK1 – 2601 Cell Signaling, Germany; Fascin – ab74487 Abcam, Germany; Cofilin – 3312 Cell Signaling, Germany; Rho-GDIα – ab108977 Epitomics, Germany; p-LIMK1/2 – sc-28409-R Santa Cruz, Germany; p-Cofilin – 3311 Cell Signalling, Germany; LIMK1 – 3842 Cell Signaling, Germany; p-Rho-GDIα – sc-33047 Santa Cruz, Germany; TNFα – 3707 Cell Signaling, Germany; p-IGF1Rβ/INSRβ – 3021 Cell Signalling, Germany; Arc – ab118929 Abcam, Germany; c-Fos – sc-52 Santa Cruz, Germany; p-CREB – 9191 Cell Signaling, Germany; CREB – 4820 Cell Signaling, Germany). .. After a washing step, blots were incubated with appropriate horseradish peroxidase-conjugated secondary antibodies in 8% milk for 1 h at room temperature and developed using the ECL system (GE Healthcare, Germany) using standard procedures.

Blocking Assay:

Article Title: The cognitive defects of neonatally irradiated mice are accompanied by changed synaptic plasticity, adult neurogenesis and neuroinflammation
Article Snippet: .. Immunoblotting analysis Hippocampal and cortical protein extracts (15 μg) were separated on 12% SDS polyacrylamide gels and were blotted to nitrocellulose membranes (GE Healthcare, Germany) via BIO-RAD criterion™ Blotter system at 100 V for 2 h. Membranes were blocked with RotiR -Block solution (Roth, Germany), washed and incubated overnight at 4°C with primary antibody dilutions as indicated by the manufacturer (GAPDH – sc-47724 Santa Cruz, Germany; CDC42 – ab106374 Abcam, Germany; Rac1 – ab33186 Abcam, Germany; p-PAK1 – 2601 Cell Signaling, Germany; Fascin – ab74487 Abcam, Germany; Cofilin – 3312 Cell Signaling, Germany; Rho-GDIα – ab108977 Epitomics, Germany; p-LIMK1/2 – sc-28409-R Santa Cruz, Germany; p-Cofilin – 3311 Cell Signalling, Germany; LIMK1 – 3842 Cell Signaling, Germany; p-Rho-GDIα – sc-33047 Santa Cruz, Germany; TNFα – 3707 Cell Signaling, Germany; p-IGF1Rβ/INSRβ – 3021 Cell Signalling, Germany; Arc – ab118929 Abcam, Germany; c-Fos – sc-52 Santa Cruz, Germany; p-CREB – 9191 Cell Signaling, Germany; CREB – 4820 Cell Signaling, Germany). .. After a washing step, blots were incubated with appropriate horseradish peroxidase-conjugated secondary antibodies in 8% milk for 1 h at room temperature and developed using the ECL system (GE Healthcare, Germany) using standard procedures.

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    Abcam fascin ab74487 abcam
    Fascin Ab74487 Abcam, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fascin ab74487 abcam/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fascin ab74487 abcam - by Bioz Stars, 2020-07
    86/100 stars
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