prerna  (Roche)


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    Structured Review

    Roche prerna
    Expression analysis of genes with CRBSs in their promoters. ( A ) UCSC browser views of BMAL1 (top), CLOCK (middle) and CRY1 (bottom) occupancy at the promoters of ATG3 , EIF5A2 , and SCN5A . Binding sites of the individual transcription regulators are indicated by colored bars. Black bars indicate common binding sites of the three regulators. ( B ) Temporal expression profiles (microarray analysis) of ATG3 , EIF5A2 , and SCN5A , which have strong CRBSs in their promoters. ( C, D ) Around the clock qRT-PCR analysis of <t>preRNA</t> ( C ) and mRNA ( D ) levels of ATG3 , EIF5A2 , and SCN5A . qRT-PCR analysis was carried out with intron- and exon-specific probes, respectively, of ATG3 , EIF5A2, and SCN5A using <t>RNA</t> of time courses A and B. Expression levels of HPRT1 were used for normalization.
    Prerna, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 16628 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prerna/product/Roche
    Average 86 stars, based on 16628 article reviews
    Price from $9.99 to $1999.99
    prerna - by Bioz Stars, 2020-12
    86/100 stars

    Images

    1) Product Images from "Non-Circadian Expression Masking Clock-Driven Weak Transcription Rhythms in U2OS Cells"

    Article Title: Non-Circadian Expression Masking Clock-Driven Weak Transcription Rhythms in U2OS Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0102238

    Expression analysis of genes with CRBSs in their promoters. ( A ) UCSC browser views of BMAL1 (top), CLOCK (middle) and CRY1 (bottom) occupancy at the promoters of ATG3 , EIF5A2 , and SCN5A . Binding sites of the individual transcription regulators are indicated by colored bars. Black bars indicate common binding sites of the three regulators. ( B ) Temporal expression profiles (microarray analysis) of ATG3 , EIF5A2 , and SCN5A , which have strong CRBSs in their promoters. ( C, D ) Around the clock qRT-PCR analysis of preRNA ( C ) and mRNA ( D ) levels of ATG3 , EIF5A2 , and SCN5A . qRT-PCR analysis was carried out with intron- and exon-specific probes, respectively, of ATG3 , EIF5A2, and SCN5A using RNA of time courses A and B. Expression levels of HPRT1 were used for normalization.
    Figure Legend Snippet: Expression analysis of genes with CRBSs in their promoters. ( A ) UCSC browser views of BMAL1 (top), CLOCK (middle) and CRY1 (bottom) occupancy at the promoters of ATG3 , EIF5A2 , and SCN5A . Binding sites of the individual transcription regulators are indicated by colored bars. Black bars indicate common binding sites of the three regulators. ( B ) Temporal expression profiles (microarray analysis) of ATG3 , EIF5A2 , and SCN5A , which have strong CRBSs in their promoters. ( C, D ) Around the clock qRT-PCR analysis of preRNA ( C ) and mRNA ( D ) levels of ATG3 , EIF5A2 , and SCN5A . qRT-PCR analysis was carried out with intron- and exon-specific probes, respectively, of ATG3 , EIF5A2, and SCN5A using RNA of time courses A and B. Expression levels of HPRT1 were used for normalization.

    Techniques Used: Expressing, Binding Assay, Microarray, Quantitative RT-PCR

    2) Product Images from "The Nuclear Effector of Wnt-Signaling, Tcf1, Functions as a T-Cell-Specific Tumor Suppressor for Development of Lymphomas"

    Article Title: The Nuclear Effector of Wnt-Signaling, Tcf1, Functions as a T-Cell-Specific Tumor Suppressor for Development of Lymphomas

    Journal: PLoS Biology

    doi: 10.1371/journal.pbio.1001430

    Tcf1 −/− lymphomas show deregulated Wnt signaling. (A) RNA isolated from thymi of 17 different mice was used for microarray analysis. Expression of Lef1, Tcf7, Myc, and Hes1 in the Tcf1 −/− mice without lymphoma ( n = 4), Tcf1 +/− mice (control, n = 5) and Tcf1 −/− mice with a lymphoma (Lymphoma, n = 8) is shown. For the Tcf1 −/− lymphoma mice, the phenotype of the tumor is indicated on the horizontal axis. Columns represent independent RNA preparations of the different mice groups. A principal component analysis was performed using Wnt and Notch target genes. A PCA analysis shows clustering of the three groups as well as the effect each of the target genes has on the separation of these groups with samples of Tcf1 +/− , Tcf1 −/− , and Tcf1 −/− with tumors indicated by red, black, and green spheres, respectively. (B) Expression levels of Tcf1, Lef1, Axin2, Cyclin D1, cMyc, Hes1, and Deltex1 as determined by Affymetrix microarray were summarized and normalized using RMA, and the expression relative to Abl was plotted for each sample. Statistical significant differences ( p
    Figure Legend Snippet: Tcf1 −/− lymphomas show deregulated Wnt signaling. (A) RNA isolated from thymi of 17 different mice was used for microarray analysis. Expression of Lef1, Tcf7, Myc, and Hes1 in the Tcf1 −/− mice without lymphoma ( n = 4), Tcf1 +/− mice (control, n = 5) and Tcf1 −/− mice with a lymphoma (Lymphoma, n = 8) is shown. For the Tcf1 −/− lymphoma mice, the phenotype of the tumor is indicated on the horizontal axis. Columns represent independent RNA preparations of the different mice groups. A principal component analysis was performed using Wnt and Notch target genes. A PCA analysis shows clustering of the three groups as well as the effect each of the target genes has on the separation of these groups with samples of Tcf1 +/− , Tcf1 −/− , and Tcf1 −/− with tumors indicated by red, black, and green spheres, respectively. (B) Expression levels of Tcf1, Lef1, Axin2, Cyclin D1, cMyc, Hes1, and Deltex1 as determined by Affymetrix microarray were summarized and normalized using RMA, and the expression relative to Abl was plotted for each sample. Statistical significant differences ( p

    Techniques Used: Isolation, Mouse Assay, Microarray, Expressing

    3) Product Images from "Spinoculation Enhances HBV Infection in NTCP-Reconstituted Hepatocytes"

    Article Title: Spinoculation Enhances HBV Infection in NTCP-Reconstituted Hepatocytes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0129889

    Centrifugal inoculation of HBV. HepG2-NTCP12 cells in 24-well-plate were inoculated by HBV (500 vge/cell) without centrifugation or at different centrifugal force (g) for 30 min. After spinoculation, the cells were transferred to regular culture condition. 7 days later, the infected cells were analyzed by HBcAg immunofluorescence (A), the levels of intracellular HBV RNA transcription and DNA replication were determined by Northern and Southern blot, respectively (B).
    Figure Legend Snippet: Centrifugal inoculation of HBV. HepG2-NTCP12 cells in 24-well-plate were inoculated by HBV (500 vge/cell) without centrifugation or at different centrifugal force (g) for 30 min. After spinoculation, the cells were transferred to regular culture condition. 7 days later, the infected cells were analyzed by HBcAg immunofluorescence (A), the levels of intracellular HBV RNA transcription and DNA replication were determined by Northern and Southern blot, respectively (B).

    Techniques Used: Centrifugation, Infection, Immunofluorescence, Northern Blot, Southern Blot

    HBV infection of HepG2-NTCP12 cells with different viral inoculum size. Cells were mock infected or infected with HBV at indicated inoculum size (vge/cell) in the presence of 2% DMSO. Seven days later, the cells were subjected to HBcAg immunofluorescence microscopy (A). The intracellular HBV RNA and core DNA were analyzed by Northern and Southern blot, respectively (B).
    Figure Legend Snippet: HBV infection of HepG2-NTCP12 cells with different viral inoculum size. Cells were mock infected or infected with HBV at indicated inoculum size (vge/cell) in the presence of 2% DMSO. Seven days later, the cells were subjected to HBcAg immunofluorescence microscopy (A). The intracellular HBV RNA and core DNA were analyzed by Northern and Southern blot, respectively (B).

    Techniques Used: Infection, Immunofluorescence, Microscopy, Northern Blot, Southern Blot

    Quantification of the genome equivalent of HBV virion DNA in concentrated HBV particles. (A) HBV virions and naked capsids in the indicated volume of virus stock were separated by native agarose gel electrophoresis, and viral DNA was detected by hybridization. The virion DNA to capsid DNA ratio was calculated by ImageQuant IQTL software using the hybridization signal intensity. (B) HBV DNA were extracted from 10 μl of virus stock and subjected to Southern blot analysis, 100 pg of 3.2 kb HBV linear DNA (approximately 3.1×10 7 HBV DNA copies quantified by qPCR) served as loading marker. HBV DNA replicative intermediates, including relaxed circular (RC) DNA and single stranded (SS) DNA were labeled. The copy number of total HBV DNA was quantified by using the HBV DNA loading marker as standard. (C) Formula for calculation of HBV virion DNA genome equivalent (v.g.e).
    Figure Legend Snippet: Quantification of the genome equivalent of HBV virion DNA in concentrated HBV particles. (A) HBV virions and naked capsids in the indicated volume of virus stock were separated by native agarose gel electrophoresis, and viral DNA was detected by hybridization. The virion DNA to capsid DNA ratio was calculated by ImageQuant IQTL software using the hybridization signal intensity. (B) HBV DNA were extracted from 10 μl of virus stock and subjected to Southern blot analysis, 100 pg of 3.2 kb HBV linear DNA (approximately 3.1×10 7 HBV DNA copies quantified by qPCR) served as loading marker. HBV DNA replicative intermediates, including relaxed circular (RC) DNA and single stranded (SS) DNA were labeled. The copy number of total HBV DNA was quantified by using the HBV DNA loading marker as standard. (C) Formula for calculation of HBV virion DNA genome equivalent (v.g.e).

    Techniques Used: Agarose Gel Electrophoresis, Hybridization, Software, Southern Blot, Real-time Polymerase Chain Reaction, Marker, Labeling

    Effect of centrifugation time on HBV spinoculation. HepG2-NTCP12 cells were inoculated with HBV (500 vge/cell) by centrifugation at 1,000×g for different time as indicated. 8 days post inoculation, HBV infection was analyzed by HBcAg immunofluorescence (A), viral RNA and core DNA hybridization (B), and HBV cccDNA produced by 60-min-spinoculation was detected by Southern blot and validated by heat denature and EcoR I linearization (C).
    Figure Legend Snippet: Effect of centrifugation time on HBV spinoculation. HepG2-NTCP12 cells were inoculated with HBV (500 vge/cell) by centrifugation at 1,000×g for different time as indicated. 8 days post inoculation, HBV infection was analyzed by HBcAg immunofluorescence (A), viral RNA and core DNA hybridization (B), and HBV cccDNA produced by 60-min-spinoculation was detected by Southern blot and validated by heat denature and EcoR I linearization (C).

    Techniques Used: Centrifugation, Infection, Immunofluorescence, DNA Hybridization, Produced, Southern Blot

    Related Articles

    Transfection:

    Article Title: Hepatitis B Viral DNA Decline at Loss of HBeAg Is Mainly Explained by Reduced cccDNA Load - Down-Regulated Transcription of PgRNA Has Limited Impact
    Article Snippet: .. Extraction of DNA and RNA from Cells DNA and RNA were extracted from transfected and non-transfected hepatoma cells in a Magnapure robot (Roche Applied Science, Germany) using the Total NA protocol. .. Harvested cells were washed in 1 mL PBS and after centrifugation at 5000 rpm for 3 min the pellet was re-suspended in 800 µL RLT lysis buffer (Qiagen Sciences, MD, USA) before extraction.

    Synthesized:

    Article Title: Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1, et al. Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1
    Article Snippet: .. 2.5 Real‐time quantitative RT‐PCR First‐strand cDNA was synthesized from the total RNA using ThermoScript RT‐PCR System (Roche, Indianapolis, IN, USA). .. PCR was performed on a LightCycler system (Roche) using LightCycler FastStart DNA Master SYBR Green I reaction mix (Roche) and QuantiTect Primer Assays (QIAGEN, Hilden, Germany).

    Isolation:

    Article Title: MERS-coronavirus replication induces severe in vitro cytopathology and is strongly inhibited by cyclosporin A or interferon-? treatment
    Article Snippet: .. RNA from 200 µl culture medium of CoV-infected cells was isolated with a MagnaPure LC Total Nucleic Acid Isolation kit (Roche) and eluted in 100 µl. .. RT-PCR conditions for quantifying MERS-CoV and SARS-CoV RNA and amplification parameters have been described previously ( ; ).

    Article Title: Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion
    Article Snippet: .. RNA purification and Real Time RT-PCR Entamoeba cells were harvested and suspended immediately in TriZol reagent (Invitrogen) and RNA was isolated and treated with DNase I (Roche) according to manufacturer's protocol. .. RNA was reverse transcribed into cDNA using Superscript III reverse transcriptase (Invitrogen) and random hexamers used as a primer.

    Article Title: Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) Total RNA was isolated from two million cells by SV total RNA isolation kit (Promega). cDNA was prepared by annealing 500 ng RNA with oligo dT as per the manufacturer’s instructions (Transcriptor High Fidelity cDNA Synthesis kit, Roche). ..

    Quantitative RT-PCR:

    Article Title: Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1, et al. Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1
    Article Snippet: .. 2.5 Real‐time quantitative RT‐PCR First‐strand cDNA was synthesized from the total RNA using ThermoScript RT‐PCR System (Roche, Indianapolis, IN, USA). .. PCR was performed on a LightCycler system (Roche) using LightCycler FastStart DNA Master SYBR Green I reaction mix (Roche) and QuantiTect Primer Assays (QIAGEN, Hilden, Germany).

    Article Title: Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion
    Article Snippet: .. RNA purification and Real Time RT-PCR Entamoeba cells were harvested and suspended immediately in TriZol reagent (Invitrogen) and RNA was isolated and treated with DNase I (Roche) according to manufacturer's protocol. .. RNA was reverse transcribed into cDNA using Superscript III reverse transcriptase (Invitrogen) and random hexamers used as a primer.

    Article Title: Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) Total RNA was isolated from two million cells by SV total RNA isolation kit (Promega). cDNA was prepared by annealing 500 ng RNA with oligo dT as per the manufacturer’s instructions (Transcriptor High Fidelity cDNA Synthesis kit, Roche). ..

    Purification:

    Article Title: Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion
    Article Snippet: .. RNA purification and Real Time RT-PCR Entamoeba cells were harvested and suspended immediately in TriZol reagent (Invitrogen) and RNA was isolated and treated with DNase I (Roche) according to manufacturer's protocol. .. RNA was reverse transcribed into cDNA using Superscript III reverse transcriptase (Invitrogen) and random hexamers used as a primer.

    Real-time Polymerase Chain Reaction:

    Article Title: Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) Total RNA was isolated from two million cells by SV total RNA isolation kit (Promega). cDNA was prepared by annealing 500 ng RNA with oligo dT as per the manufacturer’s instructions (Transcriptor High Fidelity cDNA Synthesis kit, Roche). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1, et al. Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1
    Article Snippet: .. 2.5 Real‐time quantitative RT‐PCR First‐strand cDNA was synthesized from the total RNA using ThermoScript RT‐PCR System (Roche, Indianapolis, IN, USA). .. PCR was performed on a LightCycler system (Roche) using LightCycler FastStart DNA Master SYBR Green I reaction mix (Roche) and QuantiTect Primer Assays (QIAGEN, Hilden, Germany).

    Article Title: Arabidopsis ETO1 specifically interacts with and negatively regulates type 2 1-aminocyclopropane-1-carboxylate synthases
    Article Snippet: .. RNA (1 μg) was used in each RT-PCR following the manufacturer's protocol (Titan One Tube RT-PCR System, Roche Diagnostics). ..

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    Roche fam tamra labeled probes
    Fam Tamra Labeled Probes, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fam tamra labeled probes/product/Roche
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fam tamra labeled probes - by Bioz Stars, 2020-12
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