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TaKaRa synapse rapsyn gene
Engraftment of injected hMSCs into the intestine of RIII mouse model. To determine the engraftment of hMSCs into the intestine of RIII mice and compare the efficiency between Ad-HGF modified MSCs (MSCs-HGF) and Ad-Null modified MSCs (MSCs-Null), the expression of human <t>β-globin</t> gene and β2-microglobin in radiation-injured intestinal tissue were detected by quantitative PCR (qPCR) and immunostaining, respectively. At 1, 7, 14 and 28 days post-transplantation, the expression of human β-globin gene in MSCs-Null and MSCs-HGF treated RIII mice were analyzed by qPCR and normalized to the mouse <t>RAPSYN</t> gene (A). To further confirm the engraftment of MSCs into the injured intestinal tissue, the expression of β2-microglobin was detected in MSCs-Null and MSCs-HGF treated RIII mice (B), and were quantitatively analyzed based on IOD value (C). The results are presented as the means ± SD. ** p
Synapse Rapsyn Gene, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 11650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 89 stars, based on 11650 article reviews
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synapse rapsyn gene - by Bioz Stars, 2020-12
89/100 stars

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1) Product Images from "HGF Gene Modification in Mesenchymal Stem Cells Reduces Radiation-Induced Intestinal Injury by Modulating Immunity"

Article Title: HGF Gene Modification in Mesenchymal Stem Cells Reduces Radiation-Induced Intestinal Injury by Modulating Immunity

Journal: PLoS ONE

doi: 10.1371/journal.pone.0124420

Engraftment of injected hMSCs into the intestine of RIII mouse model. To determine the engraftment of hMSCs into the intestine of RIII mice and compare the efficiency between Ad-HGF modified MSCs (MSCs-HGF) and Ad-Null modified MSCs (MSCs-Null), the expression of human β-globin gene and β2-microglobin in radiation-injured intestinal tissue were detected by quantitative PCR (qPCR) and immunostaining, respectively. At 1, 7, 14 and 28 days post-transplantation, the expression of human β-globin gene in MSCs-Null and MSCs-HGF treated RIII mice were analyzed by qPCR and normalized to the mouse RAPSYN gene (A). To further confirm the engraftment of MSCs into the injured intestinal tissue, the expression of β2-microglobin was detected in MSCs-Null and MSCs-HGF treated RIII mice (B), and were quantitatively analyzed based on IOD value (C). The results are presented as the means ± SD. ** p
Figure Legend Snippet: Engraftment of injected hMSCs into the intestine of RIII mouse model. To determine the engraftment of hMSCs into the intestine of RIII mice and compare the efficiency between Ad-HGF modified MSCs (MSCs-HGF) and Ad-Null modified MSCs (MSCs-Null), the expression of human β-globin gene and β2-microglobin in radiation-injured intestinal tissue were detected by quantitative PCR (qPCR) and immunostaining, respectively. At 1, 7, 14 and 28 days post-transplantation, the expression of human β-globin gene in MSCs-Null and MSCs-HGF treated RIII mice were analyzed by qPCR and normalized to the mouse RAPSYN gene (A). To further confirm the engraftment of MSCs into the injured intestinal tissue, the expression of β2-microglobin was detected in MSCs-Null and MSCs-HGF treated RIII mice (B), and were quantitatively analyzed based on IOD value (C). The results are presented as the means ± SD. ** p

Techniques Used: Injection, Mouse Assay, Modification, Expressing, Real-time Polymerase Chain Reaction, Immunostaining, Transplantation Assay

2) Product Images from "HGF Gene Modification in Mesenchymal Stem Cells Reduces Radiation-Induced Intestinal Injury by Modulating Immunity"

Article Title: HGF Gene Modification in Mesenchymal Stem Cells Reduces Radiation-Induced Intestinal Injury by Modulating Immunity

Journal: PLoS ONE

doi: 10.1371/journal.pone.0124420

Engraftment of injected hMSCs into the intestine of RIII mouse model. To determine the engraftment of hMSCs into the intestine of RIII mice and compare the efficiency between Ad-HGF modified MSCs (MSCs-HGF) and Ad-Null modified MSCs (MSCs-Null), the expression of human β-globin gene and β2-microglobin in radiation-injured intestinal tissue were detected by quantitative PCR (qPCR) and immunostaining, respectively. At 1, 7, 14 and 28 days post-transplantation, the expression of human β-globin gene in MSCs-Null and MSCs-HGF treated RIII mice were analyzed by qPCR and normalized to the mouse RAPSYN gene (A). To further confirm the engraftment of MSCs into the injured intestinal tissue, the expression of β2-microglobin was detected in MSCs-Null and MSCs-HGF treated RIII mice (B), and were quantitatively analyzed based on IOD value (C). The results are presented as the means ± SD. ** p
Figure Legend Snippet: Engraftment of injected hMSCs into the intestine of RIII mouse model. To determine the engraftment of hMSCs into the intestine of RIII mice and compare the efficiency between Ad-HGF modified MSCs (MSCs-HGF) and Ad-Null modified MSCs (MSCs-Null), the expression of human β-globin gene and β2-microglobin in radiation-injured intestinal tissue were detected by quantitative PCR (qPCR) and immunostaining, respectively. At 1, 7, 14 and 28 days post-transplantation, the expression of human β-globin gene in MSCs-Null and MSCs-HGF treated RIII mice were analyzed by qPCR and normalized to the mouse RAPSYN gene (A). To further confirm the engraftment of MSCs into the injured intestinal tissue, the expression of β2-microglobin was detected in MSCs-Null and MSCs-HGF treated RIII mice (B), and were quantitatively analyzed based on IOD value (C). The results are presented as the means ± SD. ** p

Techniques Used: Injection, Mouse Assay, Modification, Expressing, Real-time Polymerase Chain Reaction, Immunostaining, Transplantation Assay

3) Product Images from "Increased PADI4 expression in blood and tissues of patients with malignant tumors"

Article Title: Increased PADI4 expression in blood and tissues of patients with malignant tumors

Journal: BMC Cancer

doi: 10.1186/1471-2407-9-40

PADI4 transcription levels in tumors and corresponding healthy tissues by TaqMan quantitative PCR . cDNA templates were synthesized using an equivalent amount of total RNA for each sample. Lanes 1–2, PADI4 mRNA levels in gastric adenocarcinomas and corresponding healthy tissues; lanes 3–4, lung adenocarcinomas and corresponding healthy tissues; lanes 5–6, hepatocellular carcinomas and corresponding healthy tissues; lanes 7–8, esophageal squamous cancer cells and corresponding healthy tissues; lanes 9–10, breast cancer cells and corresponding healthy tissues; and lanes 11–12, breast fibroadenomas and corresponding healthy tissues.
Figure Legend Snippet: PADI4 transcription levels in tumors and corresponding healthy tissues by TaqMan quantitative PCR . cDNA templates were synthesized using an equivalent amount of total RNA for each sample. Lanes 1–2, PADI4 mRNA levels in gastric adenocarcinomas and corresponding healthy tissues; lanes 3–4, lung adenocarcinomas and corresponding healthy tissues; lanes 5–6, hepatocellular carcinomas and corresponding healthy tissues; lanes 7–8, esophageal squamous cancer cells and corresponding healthy tissues; lanes 9–10, breast cancer cells and corresponding healthy tissues; and lanes 11–12, breast fibroadenomas and corresponding healthy tissues.

Techniques Used: Real-time Polymerase Chain Reaction, Synthesized

Related Articles

Synthesized:

Article Title: Glucose deprivation regulates the progranulin–sortilin axis in PC12 cells
Article Snippet: .. Quantitative PCR analysis PC12 cells were differentiated as previously described, and then cultured in DMEM containing different concentrations of glucose (0, 5, or 22.5 mm ) for 0–24 h. Total RNA was isolated using a NucleoSpin™ RNA Isolation Kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer's protocol. cDNAs were synthesized from total RNA using ReverTra Ace qPCR RT Master Mix (Toyobo Co. Ltd., Osaka, Japan). .. Fluorescence real‐time PCR analysis was performed using a Step One instrument (Life Technologies Corporation, Grand Island, NY, USA) and an SYBR Green detection kit according to the manufacture's protocol (KAPA Biosystems Inc., Woburn, MA, USA).

Isolation:

Article Title: Wnt-β-Catenin Signaling Promotes the Maturation of Mast Cells
Article Snippet: .. Reverse Transcription and Quantitative Polymerase Chain Reaction (RT-PCR) Total RNA was isolated from mast cells by using RNAiso Plus reagent (Takara) and NucleoSpin RNAXS (Takara). .. The cDNA was synthesized using SuperScript VILO cDNA synthesis kit (Life Technologies), and semiquantitative PCR was then performed using TAKARA Ex Taq HS DNA polymerase (Takara).

Article Title: Glucose deprivation regulates the progranulin–sortilin axis in PC12 cells
Article Snippet: .. Quantitative PCR analysis PC12 cells were differentiated as previously described, and then cultured in DMEM containing different concentrations of glucose (0, 5, or 22.5 mm ) for 0–24 h. Total RNA was isolated using a NucleoSpin™ RNA Isolation Kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer's protocol. cDNAs were synthesized from total RNA using ReverTra Ace qPCR RT Master Mix (Toyobo Co. Ltd., Osaka, Japan). .. Fluorescence real‐time PCR analysis was performed using a Step One instrument (Life Technologies Corporation, Grand Island, NY, USA) and an SYBR Green detection kit according to the manufacture's protocol (KAPA Biosystems Inc., Woburn, MA, USA).

Article Title: Laminin-332 alters connexin profile, dye coupling and intercellular Ca2+ waves in ciliated tracheal epithelial cells
Article Snippet: .. Total RNA from remaining outgrowth cells was isolated using the NucleoSpin RNAII kit (Clontech, Mountain View, CA) as per manufacturer's protocol. .. Isolated RNA (2 μg) was used as a template for reverse transcription with a First Strand cDNA Synthesis kit (Fermentas, Inc., Hanover, MD).

Article Title: MUL1 acts in parallel to the PINK1/parkin pathway in regulating mitofusin and compensates for loss of PINK1/parkin
Article Snippet: .. RNA isolation, cDNA synthesis, and quantitative PCR (qPCR) RNA was isolated from whole flies using the Macherry-Nagel Nucleospin RNA II kit. cDNA synthesis was performed using the Clontech RNA to cDNA EcoDry Premix Kit, using a combination of Oligo-dT and random hexamer priming. .. Quantitative PCR was performed using the BioRadiTaq Fast Sybr Green enzyme mix, 10 µl reactions in triplicate, on a Roche Light Cycler 480.

Cell Culture:

Article Title: Glucose deprivation regulates the progranulin–sortilin axis in PC12 cells
Article Snippet: .. Quantitative PCR analysis PC12 cells were differentiated as previously described, and then cultured in DMEM containing different concentrations of glucose (0, 5, or 22.5 mm ) for 0–24 h. Total RNA was isolated using a NucleoSpin™ RNA Isolation Kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer's protocol. cDNAs were synthesized from total RNA using ReverTra Ace qPCR RT Master Mix (Toyobo Co. Ltd., Osaka, Japan). .. Fluorescence real‐time PCR analysis was performed using a Step One instrument (Life Technologies Corporation, Grand Island, NY, USA) and an SYBR Green detection kit according to the manufacture's protocol (KAPA Biosystems Inc., Woburn, MA, USA).

Purification:

Article Title: Increased cytoplasmic TARDBP mRNA in affected spinal motor neurons in ALS caused by abnormal autoregulation of TDP-43
Article Snippet: .. Total RNA was extracted (Nucleospin RNAII; Takara Bio) and poly(A)+ RNA was purified (NucleoTrap mRNA Mini kit; Takara Bio) according to the manufacturer's instructions. .. The RNA samples were loaded on 1.5% gels (NorthernMax-Gly Kit; Life Technologies), transferred to Hybond N+ nylon membranes (GE Healthcare) and probed with internally DIG-labeled sequences following pre-hybridization in ULTRAhyb hybridization buffer (Ambion).

Real-time Polymerase Chain Reaction:

Article Title: Wnt-β-Catenin Signaling Promotes the Maturation of Mast Cells
Article Snippet: .. Reverse Transcription and Quantitative Polymerase Chain Reaction (RT-PCR) Total RNA was isolated from mast cells by using RNAiso Plus reagent (Takara) and NucleoSpin RNAXS (Takara). .. The cDNA was synthesized using SuperScript VILO cDNA synthesis kit (Life Technologies), and semiquantitative PCR was then performed using TAKARA Ex Taq HS DNA polymerase (Takara).

Article Title: Glucose deprivation regulates the progranulin–sortilin axis in PC12 cells
Article Snippet: .. Quantitative PCR analysis PC12 cells were differentiated as previously described, and then cultured in DMEM containing different concentrations of glucose (0, 5, or 22.5 mm ) for 0–24 h. Total RNA was isolated using a NucleoSpin™ RNA Isolation Kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer's protocol. cDNAs were synthesized from total RNA using ReverTra Ace qPCR RT Master Mix (Toyobo Co. Ltd., Osaka, Japan). .. Fluorescence real‐time PCR analysis was performed using a Step One instrument (Life Technologies Corporation, Grand Island, NY, USA) and an SYBR Green detection kit according to the manufacture's protocol (KAPA Biosystems Inc., Woburn, MA, USA).

Article Title: MUL1 acts in parallel to the PINK1/parkin pathway in regulating mitofusin and compensates for loss of PINK1/parkin
Article Snippet: .. RNA isolation, cDNA synthesis, and quantitative PCR (qPCR) RNA was isolated from whole flies using the Macherry-Nagel Nucleospin RNA II kit. cDNA synthesis was performed using the Clontech RNA to cDNA EcoDry Premix Kit, using a combination of Oligo-dT and random hexamer priming. .. Quantitative PCR was performed using the BioRadiTaq Fast Sybr Green enzyme mix, 10 µl reactions in triplicate, on a Roche Light Cycler 480.

Random Hexamer Labeling:

Article Title: MUL1 acts in parallel to the PINK1/parkin pathway in regulating mitofusin and compensates for loss of PINK1/parkin
Article Snippet: .. RNA isolation, cDNA synthesis, and quantitative PCR (qPCR) RNA was isolated from whole flies using the Macherry-Nagel Nucleospin RNA II kit. cDNA synthesis was performed using the Clontech RNA to cDNA EcoDry Premix Kit, using a combination of Oligo-dT and random hexamer priming. .. Quantitative PCR was performed using the BioRadiTaq Fast Sybr Green enzyme mix, 10 µl reactions in triplicate, on a Roche Light Cycler 480.

Reverse Transcription Polymerase Chain Reaction:

Article Title: ASC Induces Apoptosis via Activation of Caspase-9 by Enhancing Gap Junction-Mediated Intercellular Communication
Article Snippet: .. Quantitative reverse-transcription polymerase chain reaction (RT-PCR) Total RNA was extracted with NucleoSpin RNAII (Takara Bio, Otsu, Japan), and RT was performed with PrimeScript® RT Master Mix (Takara Bio). .. The PCR was set up with SYBER Premix Ex Taq™ II (Takara Bio) and carried out on a Thermal Cycler Dice Real Time System II device (Takara Bio).

Article Title: Wnt-β-Catenin Signaling Promotes the Maturation of Mast Cells
Article Snippet: .. Reverse Transcription and Quantitative Polymerase Chain Reaction (RT-PCR) Total RNA was isolated from mast cells by using RNAiso Plus reagent (Takara) and NucleoSpin RNAXS (Takara). .. The cDNA was synthesized using SuperScript VILO cDNA synthesis kit (Life Technologies), and semiquantitative PCR was then performed using TAKARA Ex Taq HS DNA polymerase (Takara).

Article Title: RNA editing by ADAR1 regulates innate and antiviral immune functions in primary macrophages
Article Snippet: .. RNA was extracted using the NucleoSpin RNA XS kit (Magerey-Nagel) including the DNase I treatment step and reverse transcription performed using the PrimeScript™ RT-PCR Kit (Takara). .. A specific set of primers and probe to amplify spliced tat/rev/nef mRNA were used (forward 5′-GGATCTGTCTCTGTCTCTCTCTCCACC-3′, reverse 5′-ACAGTCAGACTCATCAAGTTTCTCTATCAAAGCA-3′ and the dual-labeled fluorescent probe FAM 5′-TTCCTTCGGGCCTGTCGGGTCCC-3′ TAMRA) and mRNA relative levels were measured by two-step quantitative RT-PCR, normalized to GAPDH mRNA expression using the DDCt method .

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    TaKaRa fam tamra labeled probe
    Fam Tamra Labeled Probe, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fam tamra labeled probe/product/TaKaRa
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fam tamra labeled probe - by Bioz Stars, 2020-12
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