factor xa  (GE Healthcare)

 
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  • 95
    Name:
    Factor Xa
    Description:

    Catalog Number:
    27084901
    Price:
    Discontinued
    Size:
    400 units
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    Structured Review

    GE Healthcare factor xa

    https://www.bioz.com/result/factor xa/product/GE Healthcare
    Average 95 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    factor xa - by Bioz Stars, 2021-01
    95/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Residues gating the periplasmic pathway of LacY
    Article Snippet: .. Cross-linking was tested as follows: aliquots (2 μg protein) were digested with factor Xa protease as described , and samples (2 μg protein) were subjected to sodium dodecyl sulfate-12% polycrylamide gel electrophoresis (SDS-12% PAGE) and western blotting with a site-directed polyclonal antibody against the C-terminus of LacY followed by horseradish peroxidase-coupled anti-rabbit antibody (Amersham Biosciences, NA-934) as described . .. The remaining sample was treated with MPB (475 μM, final concentration) on ice for given times, and the reaction was terminated by addition of 10 mM dithiothreitol (DTT).

    Flow Cytometry:

    Article Title: Domain:domain interactions within Hop, the Hsp70/Hsp90 organizing protein, are required for protein stability and structure
    Article Snippet: .. Removal of the Factor Xa protease was accomplished by applying the flowthrough of the glutathione-Sepharose column onto a 1-mL HiTrap Benzamidine Sepharose 4 fast flow column (Amersham). .. Proteins were analyzed on a precast 4%–20% gradient gel (BioRad) and quantitated by measuring protein absorbance at 280 nm.

    Affinity Chromatography:

    Article Title: The structure of a replication initiator unites diverse aspects of nucleic acid metabolism
    Article Snippet: .. The soluble fusion protein was purified by affinity chromatography, subjected to factor Xa protease digestion, and repurified by size-exclusion chromatography on a Superdex-75 column (Amersham Pharmacia). .. Purity, extent of labeling, and identity of the 15 N-labeled Rep domain was assessed by mass spectrometry (13,730 Da expected mass, 13,716 Da observed mass) and N-terminal amino acid sequencing, establishing that the cleaved protein comprised residues 4–121.

    Buffer Exchange:

    Article Title: Group X Secreted Phospholipase A2 Proenzyme Is Matured by a Furin-like Proprotein Convertase and Releases Arachidonic Acid inside of Human HEK293 Cells *
    Article Snippet: .. Briefly, the ΔGST-FXa-proenzyme proteins were produced in Escherichia coli as inclusion bodies, solubilized and sulfonated in high chaotrope, refolded by rapid dilution in a semichaotropic buffer (same buffer as mGX ( )), and cleaved by Factor Xa (GE Healthcare) after Factor Xa buffer exchange. .. All proteins were purified to homogeneity by a two-step HPLC purification using cation-exchange and reverse-phase columns, and the pure proteins were characterized by SDS-PAGE analysis, MALDI-TOF mass spectrometry, and enzymatic assays, as described for mature mGX sPLA2 ( ).

    Size-exclusion Chromatography:

    Article Title: The structure of a replication initiator unites diverse aspects of nucleic acid metabolism
    Article Snippet: .. The soluble fusion protein was purified by affinity chromatography, subjected to factor Xa protease digestion, and repurified by size-exclusion chromatography on a Superdex-75 column (Amersham Pharmacia). .. Purity, extent of labeling, and identity of the 15 N-labeled Rep domain was assessed by mass spectrometry (13,730 Da expected mass, 13,716 Da observed mass) and N-terminal amino acid sequencing, establishing that the cleaved protein comprised residues 4–121.

    Purification:

    Article Title: The structure of a replication initiator unites diverse aspects of nucleic acid metabolism
    Article Snippet: .. The soluble fusion protein was purified by affinity chromatography, subjected to factor Xa protease digestion, and repurified by size-exclusion chromatography on a Superdex-75 column (Amersham Pharmacia). .. Purity, extent of labeling, and identity of the 15 N-labeled Rep domain was assessed by mass spectrometry (13,730 Da expected mass, 13,716 Da observed mass) and N-terminal amino acid sequencing, establishing that the cleaved protein comprised residues 4–121.

    Article Title: The Flavoprotein MrsD Catalyzes the Oxidative Decarboxylation Reaction Involved in Formation of the Peptidoglycan Biosynthesis Inhibitor Mersacidin
    Article Snippet: .. Cleavage of the purified MBP-MrsD fusion protein was carried out in 20 mM Tris-HCl-200 mM NaCl buffer containing 1 U of factor Xa/100 μl (Amersham Pharmacia Biotech). .. The mix was incubated for several hours at room temperature, and cleavage was monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Produced:

    Article Title: Group X Secreted Phospholipase A2 Proenzyme Is Matured by a Furin-like Proprotein Convertase and Releases Arachidonic Acid inside of Human HEK293 Cells *
    Article Snippet: .. Briefly, the ΔGST-FXa-proenzyme proteins were produced in Escherichia coli as inclusion bodies, solubilized and sulfonated in high chaotrope, refolded by rapid dilution in a semichaotropic buffer (same buffer as mGX ( )), and cleaved by Factor Xa (GE Healthcare) after Factor Xa buffer exchange. .. All proteins were purified to homogeneity by a two-step HPLC purification using cation-exchange and reverse-phase columns, and the pure proteins were characterized by SDS-PAGE analysis, MALDI-TOF mass spectrometry, and enzymatic assays, as described for mature mGX sPLA2 ( ).

    Incubation:

    Article Title: Expression, purification, crystallization and preliminary X-ray crystallographic studies of alkyl hydroperoxide reductase (AhpC) from the cyanobacterium Anabaena sp. PCC 7120
    Article Snippet: .. For cleavage of the GST tag, the GST-AhpC fusion protein solution was incubated with 10 U mg−1 factor Xa protease (GE Healthcare, Sweden) for 16 h at 295 K. After digestion, the protein solution was loaded onto a 5 ml GSTrap FF column pre-equilibrated with 50 m M Tris–HCl, 150 m M NaCl, 1 m M CaCl2 . .. The cleaved AhpC protein was collected in the flowthrough fraction and was further purified by size-exclusion chromatography using a HiPrep 16/60 Sephacryl S-100 HR column (GE Healthcare, Sweden) equilibrated with 50 m M Tris–HCl, 150 m M NaCl, 1 m M CaCl2 .

    Article Title: Role of tryptophan anchor in human topoisomerase I structure, function, and inhibition
    Article Snippet: .. The 6-His tag was removed by Factor Xa protease (Amersham Biosciences, Uppsala, Sweden and New England BioLabs, Beverly, MA), a serine protease, during 5 hr incubation at 21°C in 5 mM CaCl2 , 100 mM NaCl, and 50 mM Tris, pH 8.0. ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: Residues gating the periplasmic pathway of LacY
    Article Snippet: .. Cross-linking was tested as follows: aliquots (2 μg protein) were digested with factor Xa protease as described , and samples (2 μg protein) were subjected to sodium dodecyl sulfate-12% polycrylamide gel electrophoresis (SDS-12% PAGE) and western blotting with a site-directed polyclonal antibody against the C-terminus of LacY followed by horseradish peroxidase-coupled anti-rabbit antibody (Amersham Biosciences, NA-934) as described . .. The remaining sample was treated with MPB (475 μM, final concentration) on ice for given times, and the reaction was terminated by addition of 10 mM dithiothreitol (DTT).

    Western Blot:

    Article Title: Residues gating the periplasmic pathway of LacY
    Article Snippet: .. Cross-linking was tested as follows: aliquots (2 μg protein) were digested with factor Xa protease as described , and samples (2 μg protein) were subjected to sodium dodecyl sulfate-12% polycrylamide gel electrophoresis (SDS-12% PAGE) and western blotting with a site-directed polyclonal antibody against the C-terminus of LacY followed by horseradish peroxidase-coupled anti-rabbit antibody (Amersham Biosciences, NA-934) as described . .. The remaining sample was treated with MPB (475 μM, final concentration) on ice for given times, and the reaction was terminated by addition of 10 mM dithiothreitol (DTT).

    Chick Chorioallantoic Membrane Assay:

    Article Title: The Recognition of Calmodulin to the Target Sequence of Calcineurin—A Novel Binding Mode
    Article Snippet: .. The linker (IEGR) in between the sequences of CaM and CaNp was then cleaved by factor Xa according to the instructions of manufacturer (GE Healthcare). .. CaNp was separated from CaM by ultrafiltration with an YM-10 membrane (Millipore-Sigma, St. Louis, MO, USA) at pH 2.

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  • 95
    GE Healthcare factor xa
    Factor Xa, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/factor xa/product/GE Healthcare
    Average 95 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    factor xa - by Bioz Stars, 2021-01
    95/100 stars
      Buy from Supplier

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