Journal: Molecular Medicine Reports

Article Title: Stigmasterol exerts antiglioma effects by regulating lipid metabolism

doi: 10.3892/mmr.2024.13351

Figure Lengend Snippet: Primers used for reverse transcription quantitative PCR.

Article Snippet: The following primary antibodies were utilized for western blotting: Rabbit anti-fatty acid binding protein 5 (FABP5) antibody (1:2,000; cat. no. 12348-1-AP; Proteintech Group, Inc.), rabbit anti-α-1B adrenergic receptor (ADRA1B) antibody (1:1,000; cat. no. 22419-1-AP; Proteintech Group, Inc.) and mouse anti-β-actin antibody (1:5,000; cat. no. T0022; Affinity Biosciences).

Techniques: Reverse Transcription, Sequencing

Journal: Molecular Medicine Reports

Article Title: Stigmasterol exerts antiglioma effects by regulating lipid metabolism

doi: 10.3892/mmr.2024.13351

Figure Lengend Snippet: Construction and validation of a prognostic model associated with stigmasterol target genes. (A) Venn diagram of potential targets of stigmasterol in the treatment of GBM, prognosis-related genes of patients with GBM and lipid metabolism-related genes. (B) Two prognosis-related target mRNAs of stigmasterol for GBM treatment were selected. (C and D) Least absolute shrinkage and selection operator variable screening process. (E) Distribution of survival status and risk score of patients with GBM in the training set. (F) Expression characteristics of the two prognosis-related target mRNAs of stigmasterol for GBM treatment in the training set. (G) ROC curve for patient survival of the training set. (H) Survival analysis between the two risk subgroups in the training set. (I) ROC curve for patient survival of the internal validation set. (J) Survival analysis between the two risk subgroups in the internal validation set. (K) ROC curve for patient survival of the external validation set. (L) Survival analysis between the two risk subgroups in the external validation set. GBM, glioblastoma; ROC, receiver operating characteristic; FABP5, fatty acid binding protein 5; ADRA1B, α-1B adrenergic receptor; AUC, area under the curve.

Article Snippet: The following primary antibodies were utilized for western blotting: Rabbit anti-fatty acid binding protein 5 (FABP5) antibody (1:2,000; cat. no. 12348-1-AP; Proteintech Group, Inc.), rabbit anti-α-1B adrenergic receptor (ADRA1B) antibody (1:1,000; cat. no. 22419-1-AP; Proteintech Group, Inc.) and mouse anti-β-actin antibody (1:5,000; cat. no. T0022; Affinity Biosciences).

Techniques: Selection, Expressing, Binding Assay

Journal: Molecular Medicine Reports

Article Title: Stigmasterol exerts antiglioma effects by regulating lipid metabolism

doi: 10.3892/mmr.2024.13351

Figure Lengend Snippet: Effects of stigmasterol on lipid metabolism and the expression of related target genes in GBM cells. (A) Effect of stigmasterol on free fatty acid levels in GBM cells. (B) Effect of stigmasterol on total cholesterol levels in GBM cells. (C) Effect of stigmasterol on the mRNA expression of the two target genes (FABP5 and ADRA1B) in GBM cells. (D) Effect of stigmasterol on the expression of the proteins encoded by the two target genes in GBM cells. (E) Molecular docking of the protein encoded by FABP5 with stigmasterol. (F) Molecular docking of the protein encoded by ADRA1B with stigmasterol. *P<0.05 and **P<0.01. GBM, glioblastoma; FABP5, fatty acid binding protein 5; ADRA1B, α-1B adrenergic receptor.

Article Snippet: The following primary antibodies were utilized for western blotting: Rabbit anti-fatty acid binding protein 5 (FABP5) antibody (1:2,000; cat. no. 12348-1-AP; Proteintech Group, Inc.), rabbit anti-α-1B adrenergic receptor (ADRA1B) antibody (1:1,000; cat. no. 22419-1-AP; Proteintech Group, Inc.) and mouse anti-β-actin antibody (1:5,000; cat. no. T0022; Affinity Biosciences).

Techniques: Expressing, Binding Assay

Journal: Molecular Medicine Reports

Article Title: Stigmasterol exerts antiglioma effects by regulating lipid metabolism

doi: 10.3892/mmr.2024.13351

Figure Lengend Snippet: Primers used for reverse transcription quantitative PCR.

Article Snippet: For detection, the following secondary antibodies were used: HRP-conjugated goat anti-rabbit immunoglobulin G (IgG) antibody (dilution, 1:5,000; cat. no. A0208; Beyotime Institute of Biotechnology) for FABP5 and ADRA1B detection, and HRP-conjugated goat anti-mouse IgG antibody (dilution, 1:5,000, cat. no. SA00001-1; Proteintech Group, Inc.) for β-actin detection.

Techniques: Reverse Transcription, Sequencing

Journal: Molecular Medicine Reports

Article Title: Stigmasterol exerts antiglioma effects by regulating lipid metabolism

doi: 10.3892/mmr.2024.13351

Figure Lengend Snippet: Construction and validation of a prognostic model associated with stigmasterol target genes. (A) Venn diagram of potential targets of stigmasterol in the treatment of GBM, prognosis-related genes of patients with GBM and lipid metabolism-related genes. (B) Two prognosis-related target mRNAs of stigmasterol for GBM treatment were selected. (C and D) Least absolute shrinkage and selection operator variable screening process. (E) Distribution of survival status and risk score of patients with GBM in the training set. (F) Expression characteristics of the two prognosis-related target mRNAs of stigmasterol for GBM treatment in the training set. (G) ROC curve for patient survival of the training set. (H) Survival analysis between the two risk subgroups in the training set. (I) ROC curve for patient survival of the internal validation set. (J) Survival analysis between the two risk subgroups in the internal validation set. (K) ROC curve for patient survival of the external validation set. (L) Survival analysis between the two risk subgroups in the external validation set. GBM, glioblastoma; ROC, receiver operating characteristic; FABP5, fatty acid binding protein 5; ADRA1B, α-1B adrenergic receptor; AUC, area under the curve.

Article Snippet: For detection, the following secondary antibodies were used: HRP-conjugated goat anti-rabbit immunoglobulin G (IgG) antibody (dilution, 1:5,000; cat. no. A0208; Beyotime Institute of Biotechnology) for FABP5 and ADRA1B detection, and HRP-conjugated goat anti-mouse IgG antibody (dilution, 1:5,000, cat. no. SA00001-1; Proteintech Group, Inc.) for β-actin detection.

Techniques: Selection, Expressing, Binding Assay

Journal: Molecular Medicine Reports

Article Title: Stigmasterol exerts antiglioma effects by regulating lipid metabolism

doi: 10.3892/mmr.2024.13351

Figure Lengend Snippet: Effects of stigmasterol on lipid metabolism and the expression of related target genes in GBM cells. (A) Effect of stigmasterol on free fatty acid levels in GBM cells. (B) Effect of stigmasterol on total cholesterol levels in GBM cells. (C) Effect of stigmasterol on the mRNA expression of the two target genes (FABP5 and ADRA1B) in GBM cells. (D) Effect of stigmasterol on the expression of the proteins encoded by the two target genes in GBM cells. (E) Molecular docking of the protein encoded by FABP5 with stigmasterol. (F) Molecular docking of the protein encoded by ADRA1B with stigmasterol. *P<0.05 and **P<0.01. GBM, glioblastoma; FABP5, fatty acid binding protein 5; ADRA1B, α-1B adrenergic receptor.

Article Snippet: For detection, the following secondary antibodies were used: HRP-conjugated goat anti-rabbit immunoglobulin G (IgG) antibody (dilution, 1:5,000; cat. no. A0208; Beyotime Institute of Biotechnology) for FABP5 and ADRA1B detection, and HRP-conjugated goat anti-mouse IgG antibody (dilution, 1:5,000, cat. no. SA00001-1; Proteintech Group, Inc.) for β-actin detection.

Techniques: Expressing, Binding Assay

Journal: Molecular Medicine Reports

Article Title: Stigmasterol exerts antiglioma effects by regulating lipid metabolism

doi: 10.3892/mmr.2024.13351

Figure Lengend Snippet: Primers used for reverse transcription quantitative PCR.

Article Snippet: For detection, the following secondary antibodies were used: HRP-conjugated goat anti-rabbit immunoglobulin G (IgG) antibody (dilution, 1:5,000; cat. no. A0208; Beyotime Institute of Biotechnology) for FABP5 and ADRA1B detection, and HRP-conjugated goat anti-mouse IgG antibody (dilution, 1:5,000, cat. no. SA00001-1; Proteintech Group, Inc.) for β-actin detection.

Techniques: Reverse Transcription, Sequencing

Journal: Molecular Medicine Reports

Article Title: Stigmasterol exerts antiglioma effects by regulating lipid metabolism

doi: 10.3892/mmr.2024.13351

Figure Lengend Snippet: Construction and validation of a prognostic model associated with stigmasterol target genes. (A) Venn diagram of potential targets of stigmasterol in the treatment of GBM, prognosis-related genes of patients with GBM and lipid metabolism-related genes. (B) Two prognosis-related target mRNAs of stigmasterol for GBM treatment were selected. (C and D) Least absolute shrinkage and selection operator variable screening process. (E) Distribution of survival status and risk score of patients with GBM in the training set. (F) Expression characteristics of the two prognosis-related target mRNAs of stigmasterol for GBM treatment in the training set. (G) ROC curve for patient survival of the training set. (H) Survival analysis between the two risk subgroups in the training set. (I) ROC curve for patient survival of the internal validation set. (J) Survival analysis between the two risk subgroups in the internal validation set. (K) ROC curve for patient survival of the external validation set. (L) Survival analysis between the two risk subgroups in the external validation set. GBM, glioblastoma; ROC, receiver operating characteristic; FABP5, fatty acid binding protein 5; ADRA1B, α-1B adrenergic receptor; AUC, area under the curve.

Article Snippet: For detection, the following secondary antibodies were used: HRP-conjugated goat anti-rabbit immunoglobulin G (IgG) antibody (dilution, 1:5,000; cat. no. A0208; Beyotime Institute of Biotechnology) for FABP5 and ADRA1B detection, and HRP-conjugated goat anti-mouse IgG antibody (dilution, 1:5,000, cat. no. SA00001-1; Proteintech Group, Inc.) for β-actin detection.

Techniques: Selection, Expressing, Binding Assay

Journal: Molecular Medicine Reports

Article Title: Stigmasterol exerts antiglioma effects by regulating lipid metabolism

doi: 10.3892/mmr.2024.13351

Figure Lengend Snippet: Effects of stigmasterol on lipid metabolism and the expression of related target genes in GBM cells. (A) Effect of stigmasterol on free fatty acid levels in GBM cells. (B) Effect of stigmasterol on total cholesterol levels in GBM cells. (C) Effect of stigmasterol on the mRNA expression of the two target genes (FABP5 and ADRA1B) in GBM cells. (D) Effect of stigmasterol on the expression of the proteins encoded by the two target genes in GBM cells. (E) Molecular docking of the protein encoded by FABP5 with stigmasterol. (F) Molecular docking of the protein encoded by ADRA1B with stigmasterol. *P<0.05 and **P<0.01. GBM, glioblastoma; FABP5, fatty acid binding protein 5; ADRA1B, α-1B adrenergic receptor.

Article Snippet: For detection, the following secondary antibodies were used: HRP-conjugated goat anti-rabbit immunoglobulin G (IgG) antibody (dilution, 1:5,000; cat. no. A0208; Beyotime Institute of Biotechnology) for FABP5 and ADRA1B detection, and HRP-conjugated goat anti-mouse IgG antibody (dilution, 1:5,000, cat. no. SA00001-1; Proteintech Group, Inc.) for β-actin detection.

Techniques: Expressing, Binding Assay