ez link tfpa peg3 biotin kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 84
    Name:
    EZ Link TFPA PEG3 Biotin
    Description:
    Thermo Scientific EZ Link TFPA PEG3 Biotin is an efficient photoactivatable reagent based on tetrafluorophenyl azide for biotinylation and includes a 3 unit polyethylene glycol PEG spacer arm Features of EZ Link TFPA PEG3 Biotin • Biomolecular labeling biotinylate proteins DNA RNA and many other macromolecules even if they do not possess primary amines or sulfhydryl groups • Photo reactive perfluorophenyl azido group activates upon exposure to ultraviolet light to form covalent bonds with nucleophiles and many other chemical groups • Pegylated spacer arm contains a hydrophilic 3 unit polyethylene glycol PEG group • Enhances solubility pegylation imparts water solubility to the biotinylated molecule helping to prevent aggregation of biotinylated antibodies stored in solution • Irreversible forms permanent thioether bonds spacer arm cannot be cleaved • Solubility best to dissolve in DMSO or DMF before further dilution in aqueous buffers • Long reach spacer arm total length added to target is 33 4 angstroms minimizing steric hindrance for binding interactions with streptavidin TFPA PEG3 Biotin is a photoactivatable reagent for biotinylation of antibodies proteins and many other kinds of macromolecules The tetrafluorophenyl azide TFPA group activates upon exposure to UV Light maximum absorptivity is at 320 nm to insert covalently at sites containing C H or N H bonds The hydrophilic polyethylene glycol PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule thus reducing aggregation of labeled molecules stored in solution The PEG spacer arm also gives this reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules We manufacture biotin reagents to ensure the highest possible overall product integrity consistency and performance for the intended research applications Biotinylation reagents differ in reactivity length solubility cell permeability and cleavability Several different types of photoreactive compounds are available Aryl azide reagents activate upon exposure to ultraviolet light initiate addition reactions with double bonds insertion into C H and N H sites or subsequent ring expansion to react with a nucleophile e g primary amines
    Catalog Number:
    21303
    Price:
    None
    Applications:
    Protein Biology|Protein Labeling|Protein Labeling & Crosslinking
    Category:
    Labeling Detection Products
    Buy from Supplier


    Structured Review

    Thermo Fisher ez link tfpa peg3 biotin kit
    Thermo Scientific EZ Link TFPA PEG3 Biotin is an efficient photoactivatable reagent based on tetrafluorophenyl azide for biotinylation and includes a 3 unit polyethylene glycol PEG spacer arm Features of EZ Link TFPA PEG3 Biotin • Biomolecular labeling biotinylate proteins DNA RNA and many other macromolecules even if they do not possess primary amines or sulfhydryl groups • Photo reactive perfluorophenyl azido group activates upon exposure to ultraviolet light to form covalent bonds with nucleophiles and many other chemical groups • Pegylated spacer arm contains a hydrophilic 3 unit polyethylene glycol PEG group • Enhances solubility pegylation imparts water solubility to the biotinylated molecule helping to prevent aggregation of biotinylated antibodies stored in solution • Irreversible forms permanent thioether bonds spacer arm cannot be cleaved • Solubility best to dissolve in DMSO or DMF before further dilution in aqueous buffers • Long reach spacer arm total length added to target is 33 4 angstroms minimizing steric hindrance for binding interactions with streptavidin TFPA PEG3 Biotin is a photoactivatable reagent for biotinylation of antibodies proteins and many other kinds of macromolecules The tetrafluorophenyl azide TFPA group activates upon exposure to UV Light maximum absorptivity is at 320 nm to insert covalently at sites containing C H or N H bonds The hydrophilic polyethylene glycol PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule thus reducing aggregation of labeled molecules stored in solution The PEG spacer arm also gives this reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules We manufacture biotin reagents to ensure the highest possible overall product integrity consistency and performance for the intended research applications Biotinylation reagents differ in reactivity length solubility cell permeability and cleavability Several different types of photoreactive compounds are available Aryl azide reagents activate upon exposure to ultraviolet light initiate addition reactions with double bonds insertion into C H and N H sites or subsequent ring expansion to react with a nucleophile e g primary amines
    https://www.bioz.com/result/ez link tfpa peg3 biotin kit/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ez link tfpa peg3 biotin kit - by Bioz Stars, 2020-01
    84/100 stars

    Related Products / Commonly Used Together

    25hc
    biotinylation

    Images

    Related Articles

    Filtration:

    Article Title: Evidence that C9ORF72 dipeptide repeat proteins associate with U2 snRNP to cause mis-splicing in ALS/FTD patients
    Article Snippet: GR, PR, or FLAG peptides were biotinylated using the EZ-Link TFPA-PEG3-Biotin kit (Thermo Fisher). .. Splicing reaction mixtures containing these peptides (10 µM) were separated on Sephacryl-S500 gel filtration columns in a buffer containing 20 mM Tris, pH 7.8, 60 mM KCl, 0.1% Triton X100, and 0.2 mM PMSF.

    In Vitro:

    Article Title: A Wheat SIMILAR TO RCD-ONE Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] [W]
    Article Snippet: Paragraph title: In Vitro Protein-DNA Binding Assay (Dot Blotting) ... The membrane was blocked with 5% (w/v) nonfat milk in 15 mL of PBST (137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2 PO4 , 8 mM Na2 HPO4 , pH 7.4, and 0.1% [v/v] Tween 20) at room temperature for 2 h. The blocked membrane was then washed with 15 mL of PBST buffer and preincubated with 15 mL of DNA probe dilution buffer (0.3% BSA and 1% goat serum in PBST) for 1 h. For DNA probe preparation, the calf thymus DNA (Sigma-Aldrich) was covalently labeled using EZ-Link TFPA-PEG3 -Biotin (Pierce), following the manufacturer’s protocol.

    Labeling:

    Article Title: A Wheat SIMILAR TO RCD-ONE Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] [W]
    Article Snippet: .. The membrane was blocked with 5% (w/v) nonfat milk in 15 mL of PBST (137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2 PO4 , 8 mM Na2 HPO4 , pH 7.4, and 0.1% [v/v] Tween 20) at room temperature for 2 h. The blocked membrane was then washed with 15 mL of PBST buffer and preincubated with 15 mL of DNA probe dilution buffer (0.3% BSA and 1% goat serum in PBST) for 1 h. For DNA probe preparation, the calf thymus DNA (Sigma-Aldrich) was covalently labeled using EZ-Link TFPA-PEG3 -Biotin (Pierce), following the manufacturer’s protocol. .. The biotinylated calf thymus DNA was then added to the probe dilution buffer to a final concentration of 10 μg/mL, followed by incubation with the membrane at room temperature for 1 h. After washing three times with PBST buffer, the membrane was incubated with streptavidin–horseradish peroxidase conjugate (Pierce) in PBST buffer for 30 min. After three washes, the specific protein-DNA binding was detected with ECL Plus Western Blotting Detection Reagents (Pierce), and images were captured by Image Quant 400 (GE Healthcare).

    Concentration Assay:

    Article Title: A Wheat SIMILAR TO RCD-ONE Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] [W]
    Article Snippet: The membrane was blocked with 5% (w/v) nonfat milk in 15 mL of PBST (137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2 PO4 , 8 mM Na2 HPO4 , pH 7.4, and 0.1% [v/v] Tween 20) at room temperature for 2 h. The blocked membrane was then washed with 15 mL of PBST buffer and preincubated with 15 mL of DNA probe dilution buffer (0.3% BSA and 1% goat serum in PBST) for 1 h. For DNA probe preparation, the calf thymus DNA (Sigma-Aldrich) was covalently labeled using EZ-Link TFPA-PEG3 -Biotin (Pierce), following the manufacturer’s protocol. .. The biotinylated calf thymus DNA was then added to the probe dilution buffer to a final concentration of 10 μg/mL, followed by incubation with the membrane at room temperature for 1 h. After washing three times with PBST buffer, the membrane was incubated with streptavidin–horseradish peroxidase conjugate (Pierce) in PBST buffer for 30 min. After three washes, the specific protein-DNA binding was detected with ECL Plus Western Blotting Detection Reagents (Pierce), and images were captured by Image Quant 400 (GE Healthcare).

    Incubation:

    Article Title: A Wheat SIMILAR TO RCD-ONE Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] [W]
    Article Snippet: The membrane was blocked with 5% (w/v) nonfat milk in 15 mL of PBST (137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2 PO4 , 8 mM Na2 HPO4 , pH 7.4, and 0.1% [v/v] Tween 20) at room temperature for 2 h. The blocked membrane was then washed with 15 mL of PBST buffer and preincubated with 15 mL of DNA probe dilution buffer (0.3% BSA and 1% goat serum in PBST) for 1 h. For DNA probe preparation, the calf thymus DNA (Sigma-Aldrich) was covalently labeled using EZ-Link TFPA-PEG3 -Biotin (Pierce), following the manufacturer’s protocol. .. The biotinylated calf thymus DNA was then added to the probe dilution buffer to a final concentration of 10 μg/mL, followed by incubation with the membrane at room temperature for 1 h. After washing three times with PBST buffer, the membrane was incubated with streptavidin–horseradish peroxidase conjugate (Pierce) in PBST buffer for 30 min. After three washes, the specific protein-DNA binding was detected with ECL Plus Western Blotting Detection Reagents (Pierce), and images were captured by Image Quant 400 (GE Healthcare).

    other:

    Article Title: Integrin activation by the lipid molecule 25-hydroxycholesterol induces a proinflammatory response
    Article Snippet: Biotinylation of 25HC The EZ-link® TFPA-PEG3-Biotin kit (Thermo Fisher Scientific, Massachusetts, USA) was used to biotinylate 25HC.

    BIA-KA:

    Article Title: An integrated experimental and modeling approach to propose biotinylated PLGA microparticles as versatile targeting vehicles for drug delivery
    Article Snippet: .. The EZ-Link®TFPA-PEG3 -Biotin, 488-streptavidin, potassium nitrate, and micro bicinchoninic acid (BCA) protein assay kit were all obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. A biotin quantification kit was bought from Pierce Biotechnology (Rockford, IL, USA).

    Western Blot:

    Article Title: Evidence that C9ORF72 dipeptide repeat proteins associate with U2 snRNP to cause mis-splicing in ALS/FTD patients
    Article Snippet: GR, PR, or FLAG peptides were biotinylated using the EZ-Link TFPA-PEG3-Biotin kit (Thermo Fisher). .. Proteins in the gel filtration fractions were analyzed on NuPAGE Novex 4–12% Bis-Tris Gels (Thermo Fisher) followed by western blotting.

    Article Title: A Wheat SIMILAR TO RCD-ONE Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] [W]
    Article Snippet: The membrane was blocked with 5% (w/v) nonfat milk in 15 mL of PBST (137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2 PO4 , 8 mM Na2 HPO4 , pH 7.4, and 0.1% [v/v] Tween 20) at room temperature for 2 h. The blocked membrane was then washed with 15 mL of PBST buffer and preincubated with 15 mL of DNA probe dilution buffer (0.3% BSA and 1% goat serum in PBST) for 1 h. For DNA probe preparation, the calf thymus DNA (Sigma-Aldrich) was covalently labeled using EZ-Link TFPA-PEG3 -Biotin (Pierce), following the manufacturer’s protocol. .. The biotinylated calf thymus DNA was then added to the probe dilution buffer to a final concentration of 10 μg/mL, followed by incubation with the membrane at room temperature for 1 h. After washing three times with PBST buffer, the membrane was incubated with streptavidin–horseradish peroxidase conjugate (Pierce) in PBST buffer for 30 min. After three washes, the specific protein-DNA binding was detected with ECL Plus Western Blotting Detection Reagents (Pierce), and images were captured by Image Quant 400 (GE Healthcare).

    Protein-DNA Binding Assay:

    Article Title: A Wheat SIMILAR TO RCD-ONE Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] [W]
    Article Snippet: Paragraph title: In Vitro Protein-DNA Binding Assay (Dot Blotting) ... The membrane was blocked with 5% (w/v) nonfat milk in 15 mL of PBST (137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2 PO4 , 8 mM Na2 HPO4 , pH 7.4, and 0.1% [v/v] Tween 20) at room temperature for 2 h. The blocked membrane was then washed with 15 mL of PBST buffer and preincubated with 15 mL of DNA probe dilution buffer (0.3% BSA and 1% goat serum in PBST) for 1 h. For DNA probe preparation, the calf thymus DNA (Sigma-Aldrich) was covalently labeled using EZ-Link TFPA-PEG3 -Biotin (Pierce), following the manufacturer’s protocol.

    Binding Assay:

    Article Title: A Wheat SIMILAR TO RCD-ONE Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity [C] [W]
    Article Snippet: The membrane was blocked with 5% (w/v) nonfat milk in 15 mL of PBST (137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2 PO4 , 8 mM Na2 HPO4 , pH 7.4, and 0.1% [v/v] Tween 20) at room temperature for 2 h. The blocked membrane was then washed with 15 mL of PBST buffer and preincubated with 15 mL of DNA probe dilution buffer (0.3% BSA and 1% goat serum in PBST) for 1 h. For DNA probe preparation, the calf thymus DNA (Sigma-Aldrich) was covalently labeled using EZ-Link TFPA-PEG3 -Biotin (Pierce), following the manufacturer’s protocol. .. The biotinylated calf thymus DNA was then added to the probe dilution buffer to a final concentration of 10 μg/mL, followed by incubation with the membrane at room temperature for 1 h. After washing three times with PBST buffer, the membrane was incubated with streptavidin–horseradish peroxidase conjugate (Pierce) in PBST buffer for 30 min. After three washes, the specific protein-DNA binding was detected with ECL Plus Western Blotting Detection Reagents (Pierce), and images were captured by Image Quant 400 (GE Healthcare).

    Staining:

    Article Title: Evidence that C9ORF72 dipeptide repeat proteins associate with U2 snRNP to cause mis-splicing in ALS/FTD patients
    Article Snippet: GR, PR, or FLAG peptides were biotinylated using the EZ-Link TFPA-PEG3-Biotin kit (Thermo Fisher). .. Total RNAs from the fractions were analyzed on an 8% denaturing polyacrylamide gel stained with ethidium bromide.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 84
    Thermo Fisher ez link tfpa peg3 biotin kit
    Ez Link Tfpa Peg3 Biotin Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ez link tfpa peg3 biotin kit/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ez link tfpa peg3 biotin kit - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    Image Search Results