ez link psoralen peg3 biotin bpso  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Name:
    EZ Link Psoralen PEG3 Biotin
    Description:
    Thermo Scientific EZ Link Psoralen PEG3 Biotin enables biotinylation of DNA or RNA via UV light activated intercalation of the psoralen group with thymine and other pyrimidine containing bases to form a covalent bonds Features of EZ Link Psoralen PEG3 Biotin • Biomolecular labeling biotinylate DNA or RNA approximately 1 biotin per 10 to 20 bases without interfering with hybridization • Photo reactive covalent attachment occurs in the presence of UV light 350nm for 10 to 30 minutes • Psoralen activated reacts through a cyclo addition mechanism with the 5 6 double bond in thymine and other pyrimidine containing bases • Pegylated spacer arm contains a hydrophilic 3 unit polyethylene glycol PEG group • Enhances solubility pegylation imparts water solubility to the biotinylated molecule helping to prevent aggregation of biotinylated antibodies stored in solution • Long reach spacer arm total length added to target is 36 9 angstroms minimizing steric hindrance for binding interactions with streptavidin Psoralen PEG3 Biotin is a photoactivatable reagent for biotinylation of DNA or RNA Upon activation with UV Light 350nm the psoralen group reacts with thymine and other pyrimidine containing bases to form a covalent bond The hydrophilic polyethylene glycol PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule thus reducing aggregation of labeled proteins stored in solution The PEG spacer arm also gives this reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules We manufacture biotin reagents to ensure the highest possible overall product integrity consistency and performance for the intended research applications Applications • Selective labeling of cell surface proteins Ref 1 • Biotinylation of hyaluronan Ref 2 • Labeling of endotoxin for receptor binding studies Ref 3
    Catalog Number:
    29986
    Price:
    None
    Applications:
    Protein Biology|Protein Labeling|Protein Labeling & Crosslinking
    Category:
    Labeling Detection Products
    Buy from Supplier


    Structured Review

    Thermo Fisher ez link psoralen peg3 biotin bpso
    Thermo Scientific EZ Link Psoralen PEG3 Biotin enables biotinylation of DNA or RNA via UV light activated intercalation of the psoralen group with thymine and other pyrimidine containing bases to form a covalent bonds Features of EZ Link Psoralen PEG3 Biotin • Biomolecular labeling biotinylate DNA or RNA approximately 1 biotin per 10 to 20 bases without interfering with hybridization • Photo reactive covalent attachment occurs in the presence of UV light 350nm for 10 to 30 minutes • Psoralen activated reacts through a cyclo addition mechanism with the 5 6 double bond in thymine and other pyrimidine containing bases • Pegylated spacer arm contains a hydrophilic 3 unit polyethylene glycol PEG group • Enhances solubility pegylation imparts water solubility to the biotinylated molecule helping to prevent aggregation of biotinylated antibodies stored in solution • Long reach spacer arm total length added to target is 36 9 angstroms minimizing steric hindrance for binding interactions with streptavidin Psoralen PEG3 Biotin is a photoactivatable reagent for biotinylation of DNA or RNA Upon activation with UV Light 350nm the psoralen group reacts with thymine and other pyrimidine containing bases to form a covalent bond The hydrophilic polyethylene glycol PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule thus reducing aggregation of labeled proteins stored in solution The PEG spacer arm also gives this reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules We manufacture biotin reagents to ensure the highest possible overall product integrity consistency and performance for the intended research applications Applications • Selective labeling of cell surface proteins Ref 1 • Biotinylation of hyaluronan Ref 2 • Labeling of endotoxin for receptor binding studies Ref 3
    https://www.bioz.com/result/ez link psoralen peg3 biotin bpso/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ez link psoralen peg3 biotin bpso - by Bioz Stars, 2020-01
    90/100 stars

    Related Products / Commonly Used Together

    pbs −

    Images

    Related Articles

    Clone Assay:

    Article Title: Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts
    Article Snippet: PCR products were cloned into TOPO® pcr4 vector followed by transformation with TOP-10 chemically competent cells. .. 50–100 ng of purified) inserts were biotinylated using the EZ-Link™ Psoralen-PEG3-Biotin (ThermoFisher Scientific).

    Centrifugation:

    Article Title: Structural alteration of DNA induced by viral protein R of HIV-1 triggers the DNA damage response
    Article Snippet: Nuclear pellet was suspended in PBS (−) supplemented with 5 μM of Ez-Link Psoralen-PEG3 -Biotin (bPso) (Thermo scientific) for 30 min at 4 °C. .. After centrifugation at 500×g at 4 °C for 10 min, pellet was suspended in 100 μl of PBS (−) and removed to 96-well plate.

    Luciferase:

    Article Title: The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response
    Article Snippet: .. Reagents and antibodies Poly(I:C), LPS, R837, PGN, chloroquine (Invivogen); TNFα, IL-1β (R & D Systems); D-galactosamine (Sigma); GM-CSF (peproTech); ELISA kit for murine IFN-β, TNFα, IL-6 (Biolegend); dual-specific luciferase assay kit (Promega); EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); lysosome isolation kit (sigma); mouse monoclonal antibodies against Flag, β-actin (Sigma), HA (OriGene), phospho-IBα (Cell Signaling Technology), TLR3 (IMGENEX); rabbit monoclonal antibodies against TLR3, phospho-IRF3 (Cell Signaling Technology); rabbit polyclonal antibodies against IRF3 (Santa Cruz Biotechnology), TRIF (Cell Signaling Technology), TBK1 and phospho-TBK1(EPITOMICS), Flag (MBL); Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody, Alexa Fluor 647-conjugated goat anti-mouse IgG antibody (Invitrogen) were purchased from the indicated manufacturers. .. Mouse antisera against human Mex3B were raised against recombinant human Mex3B fragments containing aa236-517 and aa1-517, respectively.

    Article Title: The heterogeneous nuclear ribonucleoprotein hnRNPM inhibits RNA virus-triggered innate immunity by antagonizing RNA sensing of RIG-I-like receptors
    Article Snippet: .. Reagents, antibodies, cells and viruses Poly(dA:dT), poly(I:C)-HWM, poly(I:C)-LWM, poly(I:C)-fluorescein, 5’ppp-dsRNA, RNase inhibitor, M-MLV and Lipofectamine 2000 (Invivogen); HT-DNA (Sigma); DTT (Fermentas); RNase I (Ambion); polybrene (Millipore); recombinant IFN-β (PeproTech); SYBR (Bio-Rad); dual-specific luciferase assay kit (Promega); puromycin and EZ-link psoralen-PEG3 -biotin and streptavidin agarose resin (Thermo); protein G sepharose (GE Healthcare); anti-Flag affinity gel (Biomake, B73102); RNAiso plus (Takara); and recombinant IFN-β (R & D systems) were purchased from the indicated companies. .. Anti-Flag (F3165), anti-β-actin (A2228) and anti-β-tubulin (T8328) were from Sigma-Aldrich.

    Article Title: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
    Article Snippet: .. Reagents, antibodies, cells, and viruses Poly(dA:dT), 2′ 3′-cGAMP, and Lipofectamine 2000 (InvivoGen); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin (Sigma); puromycin and EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6 (BioLegend), and CXCL10 (BOSTER); recombinant IFN-β (R & D systems) were purchased for the indicated manufacturers. .. Mouse monoclonal antibodies against HA (BioLegend, 901515; 1:2000), FLAG (Sigma, F3165; 1:2000) and β-actin (Sigma, A2228; 1:3000) and phosphor-IκBα (Cell Signaling Technology, 9246 S; 1:1000); rabbit monoclonal antibodies against cGAS (Cell Signaling Technology, 66546 S/31659 S; 1:1000), phosphor-Tyrosine701-STAT1 (Cell Signaling Technology, 9167 S; 1:1000) and phosphor-IRF3 (Cell Signaling Technology, 4947 S; 1:1000), phosphor-TBK1 (Abcam, ab109272; 1:1000) and TBK1(Abcam, ab40676; 1:2000), IRF3 (Santa Cruz Biotechnology, sc-33641; 1:1000) and STAT1 (Santa Cruz Biotechnology, sc-417; 1:1000) were purchased from the indicated manufacturers.

    Article Title: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
    Article Snippet: .. Poly(dA:dT), 2′ 3′-cGAMP, and Lipofectamine 2000 (InvivoGen); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin (Sigma); puromycin and EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6 (BioLegend), and CXCL10 (BOSTER); recombinant IFN-β (R & D systems) were purchased for the indicated manufacturers. .. Mouse monoclonal antibodies against HA (BioLegend, 901515; 1:2000), FLAG (Sigma, F3165; 1:2000) and β-actin (Sigma, A2228; 1:3000) and phosphor-IκBα (Cell Signaling Technology, 9246 S; 1:1000); rabbit monoclonal antibodies against cGAS (Cell Signaling Technology, 66546 S/31659 S; 1:1000), phosphor-Tyrosine701-STAT1 (Cell Signaling Technology, 9167 S; 1:1000) and phosphor-IRF3 (Cell Signaling Technology, 4947 S; 1:1000), phosphor-TBK1 (Abcam, ab109272; 1:1000) and TBK1(Abcam, ab40676; 1:2000), IRF3 (Santa Cruz Biotechnology, sc-33641; 1:1000) and STAT1 (Santa Cruz Biotechnology, sc-417; 1:1000) were purchased from the indicated manufacturers.

    Enzyme-linked Immunosorbent Assay:

    Article Title: The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response
    Article Snippet: .. Reagents and antibodies Poly(I:C), LPS, R837, PGN, chloroquine (Invivogen); TNFα, IL-1β (R & D Systems); D-galactosamine (Sigma); GM-CSF (peproTech); ELISA kit for murine IFN-β, TNFα, IL-6 (Biolegend); dual-specific luciferase assay kit (Promega); EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); lysosome isolation kit (sigma); mouse monoclonal antibodies against Flag, β-actin (Sigma), HA (OriGene), phospho-IBα (Cell Signaling Technology), TLR3 (IMGENEX); rabbit monoclonal antibodies against TLR3, phospho-IRF3 (Cell Signaling Technology); rabbit polyclonal antibodies against IRF3 (Santa Cruz Biotechnology), TRIF (Cell Signaling Technology), TBK1 and phospho-TBK1(EPITOMICS), Flag (MBL); Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody, Alexa Fluor 647-conjugated goat anti-mouse IgG antibody (Invitrogen) were purchased from the indicated manufacturers. .. Mouse antisera against human Mex3B were raised against recombinant human Mex3B fragments containing aa236-517 and aa1-517, respectively.

    Article Title: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
    Article Snippet: .. Reagents, antibodies, cells, and viruses Poly(dA:dT), 2′ 3′-cGAMP, and Lipofectamine 2000 (InvivoGen); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin (Sigma); puromycin and EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6 (BioLegend), and CXCL10 (BOSTER); recombinant IFN-β (R & D systems) were purchased for the indicated manufacturers. .. Mouse monoclonal antibodies against HA (BioLegend, 901515; 1:2000), FLAG (Sigma, F3165; 1:2000) and β-actin (Sigma, A2228; 1:3000) and phosphor-IκBα (Cell Signaling Technology, 9246 S; 1:1000); rabbit monoclonal antibodies against cGAS (Cell Signaling Technology, 66546 S/31659 S; 1:1000), phosphor-Tyrosine701-STAT1 (Cell Signaling Technology, 9167 S; 1:1000) and phosphor-IRF3 (Cell Signaling Technology, 4947 S; 1:1000), phosphor-TBK1 (Abcam, ab109272; 1:1000) and TBK1(Abcam, ab40676; 1:2000), IRF3 (Santa Cruz Biotechnology, sc-33641; 1:1000) and STAT1 (Santa Cruz Biotechnology, sc-417; 1:1000) were purchased from the indicated manufacturers.

    Article Title: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
    Article Snippet: .. Poly(dA:dT), 2′ 3′-cGAMP, and Lipofectamine 2000 (InvivoGen); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin (Sigma); puromycin and EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6 (BioLegend), and CXCL10 (BOSTER); recombinant IFN-β (R & D systems) were purchased for the indicated manufacturers. .. Mouse monoclonal antibodies against HA (BioLegend, 901515; 1:2000), FLAG (Sigma, F3165; 1:2000) and β-actin (Sigma, A2228; 1:3000) and phosphor-IκBα (Cell Signaling Technology, 9246 S; 1:1000); rabbit monoclonal antibodies against cGAS (Cell Signaling Technology, 66546 S/31659 S; 1:1000), phosphor-Tyrosine701-STAT1 (Cell Signaling Technology, 9167 S; 1:1000) and phosphor-IRF3 (Cell Signaling Technology, 4947 S; 1:1000), phosphor-TBK1 (Abcam, ab109272; 1:1000) and TBK1(Abcam, ab40676; 1:2000), IRF3 (Santa Cruz Biotechnology, sc-33641; 1:1000) and STAT1 (Santa Cruz Biotechnology, sc-417; 1:1000) were purchased from the indicated manufacturers.

    Incubation:

    Article Title: Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates
    Article Snippet: Poly(I:C) was conjugated with biotin using an EZ-Link Psoralen-PEG3-Biotin kit (Thermo, USA) by exposure to UV (365 nm wavelength), according to the manufacturer’s instructions. .. Lysates from 293 cells transfected with the indicated plasmids were incubated with biotinylated-poly(I:C) for 1 h at room temperature and then incubated with streptavidin beads for a further 45 min at 4 °C.

    Transformation Assay:

    Article Title: Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts
    Article Snippet: PCR products were cloned into TOPO® pcr4 vector followed by transformation with TOP-10 chemically competent cells. .. 50–100 ng of purified) inserts were biotinylated using the EZ-Link™ Psoralen-PEG3-Biotin (ThermoFisher Scientific).

    Hybridization:

    Article Title: Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts
    Article Snippet: 50–100 ng of purified) inserts were biotinylated using the EZ-Link™ Psoralen-PEG3-Biotin (ThermoFisher Scientific). .. Membranes were pre-hybridized at 55 °C in North2South hybridization buffer (Pierce) for 30 min followed by overnight hybridization with denatured biotinylated probes at 5 ng/ml in the same buffer at 55 °C.

    Article Title: Low-cost cross-taxon enrichment of mitochondrial DNA using in-house synthesised RNA probes
    Article Snippet: Biotinylation of fragmented RNA Fragmented RNA was photolabelled with biotin using the EZ-Link Psoralen-PEG3-Biotin reagent (ThermoFisher) and the manufacturer's instructions. .. The labelled RNA was purified with a RNeasy MinElute Cleanup kit, quantified with a Qubit RNA BR assay (ThermoFisher), and separated into 125 ng aliquots for use in single hybridization capture reactions.

    Transfection:

    Article Title: Structural alteration of DNA induced by viral protein R of HIV-1 triggers the DNA damage response
    Article Snippet: After 2 days of transfection, 5 × 106 cells were suspended in 250 μl of RSB (10 mM Tris [pH 7.5], 10 mM NaCl, 3 mM MgCl2 ) and combined with 3.5 ml of RSB with 0.1% NP40. .. Nuclear pellet was suspended in PBS (−) supplemented with 5 μM of Ez-Link Psoralen-PEG3 -Biotin (bPso) (Thermo scientific) for 30 min at 4 °C.

    Article Title: Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates
    Article Snippet: Poly(I:C) was conjugated with biotin using an EZ-Link Psoralen-PEG3-Biotin kit (Thermo, USA) by exposure to UV (365 nm wavelength), according to the manufacturer’s instructions. .. Lysates from 293 cells transfected with the indicated plasmids were incubated with biotinylated-poly(I:C) for 1 h at room temperature and then incubated with streptavidin beads for a further 45 min at 4 °C.

    Protease Inhibitor:

    Article Title: Structural alteration of DNA induced by viral protein R of HIV-1 triggers the DNA damage response
    Article Snippet: Nuclear pellet was suspended in PBS (−) supplemented with 5 μM of Ez-Link Psoralen-PEG3 -Biotin (bPso) (Thermo scientific) for 30 min at 4 °C. .. For cross-linking the bPso to DNA, 365 nm wavelength of UV (UVL-21, UVP) was irradiated for 30 min on ice, and then collected nuclei were lysed in sonication buffer (50 mM Tris [pH 7.5], 140 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% TritonX-100, 0.1% DOC, 0.1% SDS) supplemented with 1× protease inhibitor cocktail.

    Northern Blot:

    Article Title: Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts
    Article Snippet: Paragraph title: Northern blotting ... 50–100 ng of purified) inserts were biotinylated using the EZ-Link™ Psoralen-PEG3-Biotin (ThermoFisher Scientific).

    Generated:

    Article Title: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
    Article Snippet: Reagents, antibodies, cells, and viruses Poly(dA:dT), 2′ 3′-cGAMP, and Lipofectamine 2000 (InvivoGen); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin (Sigma); puromycin and EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6 (BioLegend), and CXCL10 (BOSTER); recombinant IFN-β (R & D systems) were purchased for the indicated manufacturers. .. Antisera against ZCCHC3 were generated by immunizing rabbits or mice with purified recombinant ZCCHC3(133-404) (1:1000).

    Article Title: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
    Article Snippet: Poly(dA:dT), 2′ 3′-cGAMP, and Lipofectamine 2000 (InvivoGen); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin (Sigma); puromycin and EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6 (BioLegend), and CXCL10 (BOSTER); recombinant IFN-β (R & D systems) were purchased for the indicated manufacturers. .. Antisera against ZCCHC3 were generated by immunizing rabbits or mice with purified recombinant ZCCHC3(133-404) (1:1000).

    Polymerase Chain Reaction:

    Article Title: Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts
    Article Snippet: PCR products were cloned into TOPO® pcr4 vector followed by transformation with TOP-10 chemically competent cells. .. 50–100 ng of purified) inserts were biotinylated using the EZ-Link™ Psoralen-PEG3-Biotin (ThermoFisher Scientific).

    Sonication:

    Article Title: Structural alteration of DNA induced by viral protein R of HIV-1 triggers the DNA damage response
    Article Snippet: Nuclear pellet was suspended in PBS (−) supplemented with 5 μM of Ez-Link Psoralen-PEG3 -Biotin (bPso) (Thermo scientific) for 30 min at 4 °C. .. For cross-linking the bPso to DNA, 365 nm wavelength of UV (UVL-21, UVP) was irradiated for 30 min on ice, and then collected nuclei were lysed in sonication buffer (50 mM Tris [pH 7.5], 140 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% TritonX-100, 0.1% DOC, 0.1% SDS) supplemented with 1× protease inhibitor cocktail.

    Recombinant:

    Article Title: The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response
    Article Snippet: Reagents and antibodies Poly(I:C), LPS, R837, PGN, chloroquine (Invivogen); TNFα, IL-1β (R & D Systems); D-galactosamine (Sigma); GM-CSF (peproTech); ELISA kit for murine IFN-β, TNFα, IL-6 (Biolegend); dual-specific luciferase assay kit (Promega); EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); lysosome isolation kit (sigma); mouse monoclonal antibodies against Flag, β-actin (Sigma), HA (OriGene), phospho-IBα (Cell Signaling Technology), TLR3 (IMGENEX); rabbit monoclonal antibodies against TLR3, phospho-IRF3 (Cell Signaling Technology); rabbit polyclonal antibodies against IRF3 (Santa Cruz Biotechnology), TRIF (Cell Signaling Technology), TBK1 and phospho-TBK1(EPITOMICS), Flag (MBL); Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody, Alexa Fluor 647-conjugated goat anti-mouse IgG antibody (Invitrogen) were purchased from the indicated manufacturers. .. Mouse antisera against human Mex3B were raised against recombinant human Mex3B fragments containing aa236-517 and aa1-517, respectively.

    Article Title: The heterogeneous nuclear ribonucleoprotein hnRNPM inhibits RNA virus-triggered innate immunity by antagonizing RNA sensing of RIG-I-like receptors
    Article Snippet: .. Reagents, antibodies, cells and viruses Poly(dA:dT), poly(I:C)-HWM, poly(I:C)-LWM, poly(I:C)-fluorescein, 5’ppp-dsRNA, RNase inhibitor, M-MLV and Lipofectamine 2000 (Invivogen); HT-DNA (Sigma); DTT (Fermentas); RNase I (Ambion); polybrene (Millipore); recombinant IFN-β (PeproTech); SYBR (Bio-Rad); dual-specific luciferase assay kit (Promega); puromycin and EZ-link psoralen-PEG3 -biotin and streptavidin agarose resin (Thermo); protein G sepharose (GE Healthcare); anti-Flag affinity gel (Biomake, B73102); RNAiso plus (Takara); and recombinant IFN-β (R & D systems) were purchased from the indicated companies. .. Anti-Flag (F3165), anti-β-actin (A2228) and anti-β-tubulin (T8328) were from Sigma-Aldrich.

    Article Title: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
    Article Snippet: .. Reagents, antibodies, cells, and viruses Poly(dA:dT), 2′ 3′-cGAMP, and Lipofectamine 2000 (InvivoGen); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin (Sigma); puromycin and EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6 (BioLegend), and CXCL10 (BOSTER); recombinant IFN-β (R & D systems) were purchased for the indicated manufacturers. .. Mouse monoclonal antibodies against HA (BioLegend, 901515; 1:2000), FLAG (Sigma, F3165; 1:2000) and β-actin (Sigma, A2228; 1:3000) and phosphor-IκBα (Cell Signaling Technology, 9246 S; 1:1000); rabbit monoclonal antibodies against cGAS (Cell Signaling Technology, 66546 S/31659 S; 1:1000), phosphor-Tyrosine701-STAT1 (Cell Signaling Technology, 9167 S; 1:1000) and phosphor-IRF3 (Cell Signaling Technology, 4947 S; 1:1000), phosphor-TBK1 (Abcam, ab109272; 1:1000) and TBK1(Abcam, ab40676; 1:2000), IRF3 (Santa Cruz Biotechnology, sc-33641; 1:1000) and STAT1 (Santa Cruz Biotechnology, sc-417; 1:1000) were purchased from the indicated manufacturers.

    Article Title: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
    Article Snippet: .. Poly(dA:dT), 2′ 3′-cGAMP, and Lipofectamine 2000 (InvivoGen); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin (Sigma); puromycin and EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6 (BioLegend), and CXCL10 (BOSTER); recombinant IFN-β (R & D systems) were purchased for the indicated manufacturers. .. Mouse monoclonal antibodies against HA (BioLegend, 901515; 1:2000), FLAG (Sigma, F3165; 1:2000) and β-actin (Sigma, A2228; 1:3000) and phosphor-IκBα (Cell Signaling Technology, 9246 S; 1:1000); rabbit monoclonal antibodies against cGAS (Cell Signaling Technology, 66546 S/31659 S; 1:1000), phosphor-Tyrosine701-STAT1 (Cell Signaling Technology, 9167 S; 1:1000) and phosphor-IRF3 (Cell Signaling Technology, 4947 S; 1:1000), phosphor-TBK1 (Abcam, ab109272; 1:1000) and TBK1(Abcam, ab40676; 1:2000), IRF3 (Santa Cruz Biotechnology, sc-33641; 1:1000) and STAT1 (Santa Cruz Biotechnology, sc-417; 1:1000) were purchased from the indicated manufacturers.

    Article Title: A Naturally Occurring Deletion in the Effector Domain of H5N1 Swine Influenza Virus Nonstructural Protein 1 Regulates Viral Fitness and Host Innate Immunity
    Article Snippet: Antibodies against NDV were prepared in our laboratory by immunizing chickens with the appropriate expressed recombinant proteins. .. Streptavidin agarose resin and EZ-Link psoralen-PEG3-biotin were purchased from Thermo Scientific (Shanghai, China).

    Pull Down Assay:

    Article Title: Structural alteration of DNA induced by viral protein R of HIV-1 triggers the DNA damage response
    Article Snippet: Paragraph title: Pull-down assay of negative supercoiled DNA ... Nuclear pellet was suspended in PBS (−) supplemented with 5 μM of Ez-Link Psoralen-PEG3 -Biotin (bPso) (Thermo scientific) for 30 min at 4 °C.

    Article Title: Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates
    Article Snippet: Paragraph title: Poly(I:C) pull-down assay ... Poly(I:C) was conjugated with biotin using an EZ-Link Psoralen-PEG3-Biotin kit (Thermo, USA) by exposure to UV (365 nm wavelength), according to the manufacturer’s instructions.

    Isolation:

    Article Title: The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response
    Article Snippet: .. Reagents and antibodies Poly(I:C), LPS, R837, PGN, chloroquine (Invivogen); TNFα, IL-1β (R & D Systems); D-galactosamine (Sigma); GM-CSF (peproTech); ELISA kit for murine IFN-β, TNFα, IL-6 (Biolegend); dual-specific luciferase assay kit (Promega); EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); lysosome isolation kit (sigma); mouse monoclonal antibodies against Flag, β-actin (Sigma), HA (OriGene), phospho-IBα (Cell Signaling Technology), TLR3 (IMGENEX); rabbit monoclonal antibodies against TLR3, phospho-IRF3 (Cell Signaling Technology); rabbit polyclonal antibodies against IRF3 (Santa Cruz Biotechnology), TRIF (Cell Signaling Technology), TBK1 and phospho-TBK1(EPITOMICS), Flag (MBL); Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody, Alexa Fluor 647-conjugated goat anti-mouse IgG antibody (Invitrogen) were purchased from the indicated manufacturers. .. Mouse antisera against human Mex3B were raised against recombinant human Mex3B fragments containing aa236-517 and aa1-517, respectively.

    Article Title: Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts
    Article Snippet: For probe preparation, bacteria were grown in Terrific Broth (Fisher) with ampicillin at 50 μg/ml, and plasmids were isolated with a QuickLyse Kit (Qiagen). .. 50–100 ng of purified) inserts were biotinylated using the EZ-Link™ Psoralen-PEG3-Biotin (ThermoFisher Scientific).

    Purification:

    Article Title: Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts
    Article Snippet: .. 50–100 ng of purified) inserts were biotinylated using the EZ-Link™ Psoralen-PEG3-Biotin (ThermoFisher Scientific). .. Membranes were pre-hybridized at 55 °C in North2South hybridization buffer (Pierce) for 30 min followed by overnight hybridization with denatured biotinylated probes at 5 ng/ml in the same buffer at 55 °C.

    Article Title: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
    Article Snippet: Reagents, antibodies, cells, and viruses Poly(dA:dT), 2′ 3′-cGAMP, and Lipofectamine 2000 (InvivoGen); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin (Sigma); puromycin and EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6 (BioLegend), and CXCL10 (BOSTER); recombinant IFN-β (R & D systems) were purchased for the indicated manufacturers. .. Antisera against ZCCHC3 were generated by immunizing rabbits or mice with purified recombinant ZCCHC3(133-404) (1:1000).

    Article Title: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
    Article Snippet: Poly(dA:dT), 2′ 3′-cGAMP, and Lipofectamine 2000 (InvivoGen); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin (Sigma); puromycin and EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6 (BioLegend), and CXCL10 (BOSTER); recombinant IFN-β (R & D systems) were purchased for the indicated manufacturers. .. Antisera against ZCCHC3 were generated by immunizing rabbits or mice with purified recombinant ZCCHC3(133-404) (1:1000).

    Article Title: Low-cost cross-taxon enrichment of mitochondrial DNA using in-house synthesised RNA probes
    Article Snippet: Biotinylation of fragmented RNA Fragmented RNA was photolabelled with biotin using the EZ-Link Psoralen-PEG3-Biotin reagent (ThermoFisher) and the manufacturer's instructions. .. The labelled RNA was purified with a RNeasy MinElute Cleanup kit, quantified with a Qubit RNA BR assay (ThermoFisher), and separated into 125 ng aliquots for use in single hybridization capture reactions.

    Gel Extraction:

    Article Title: Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts
    Article Snippet: Inserts were released with BamH I/EcoR I for 25S and 5S, and BamH I/NcoI for 18S, and were purified with a QIAquick Gel Extraction Kit (Qiagen). .. 50–100 ng of purified) inserts were biotinylated using the EZ-Link™ Psoralen-PEG3-Biotin (ThermoFisher Scientific).

    Mouse Assay:

    Article Title: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
    Article Snippet: Reagents, antibodies, cells, and viruses Poly(dA:dT), 2′ 3′-cGAMP, and Lipofectamine 2000 (InvivoGen); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin (Sigma); puromycin and EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6 (BioLegend), and CXCL10 (BOSTER); recombinant IFN-β (R & D systems) were purchased for the indicated manufacturers. .. Antisera against ZCCHC3 were generated by immunizing rabbits or mice with purified recombinant ZCCHC3(133-404) (1:1000).

    Article Title: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
    Article Snippet: Poly(dA:dT), 2′ 3′-cGAMP, and Lipofectamine 2000 (InvivoGen); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin (Sigma); puromycin and EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6 (BioLegend), and CXCL10 (BOSTER); recombinant IFN-β (R & D systems) were purchased for the indicated manufacturers. .. Antisera against ZCCHC3 were generated by immunizing rabbits or mice with purified recombinant ZCCHC3(133-404) (1:1000).

    Plasmid Preparation:

    Article Title: Structural alteration of DNA induced by viral protein R of HIV-1 triggers the DNA damage response
    Article Snippet: Pull-down assay of negative supercoiled DNA U2OS/2-6-3 cells were transfected with indicated plasmid DNA by Viafect transfection reagent (Promega). .. Nuclear pellet was suspended in PBS (−) supplemented with 5 μM of Ez-Link Psoralen-PEG3 -Biotin (bPso) (Thermo scientific) for 30 min at 4 °C.

    Article Title: Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts
    Article Snippet: PCR products were cloned into TOPO® pcr4 vector followed by transformation with TOP-10 chemically competent cells. .. 50–100 ng of purified) inserts were biotinylated using the EZ-Link™ Psoralen-PEG3-Biotin (ThermoFisher Scientific).

    Irradiation:

    Article Title: Structural alteration of DNA induced by viral protein R of HIV-1 triggers the DNA damage response
    Article Snippet: Nuclear pellet was suspended in PBS (−) supplemented with 5 μM of Ez-Link Psoralen-PEG3 -Biotin (bPso) (Thermo scientific) for 30 min at 4 °C. .. For cross-linking the bPso to DNA, 365 nm wavelength of UV (UVL-21, UVP) was irradiated for 30 min on ice, and then collected nuclei were lysed in sonication buffer (50 mM Tris [pH 7.5], 140 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% TritonX-100, 0.1% DOC, 0.1% SDS) supplemented with 1× protease inhibitor cocktail.

    In Vitro:

    Article Title: Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates
    Article Snippet: Poly(I:C) pull-down assay We performed an in vitro poly(I:C) pull-down assay to detect the interaction between this synthetic dsRNA viral analogue poly(I:C) and oyster RIG-I-1 protein. .. Poly(I:C) was conjugated with biotin using an EZ-Link Psoralen-PEG3-Biotin kit (Thermo, USA) by exposure to UV (365 nm wavelength), according to the manufacturer’s instructions.

    Lysis:

    Article Title: Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates
    Article Snippet: Poly(I:C) was conjugated with biotin using an EZ-Link Psoralen-PEG3-Biotin kit (Thermo, USA) by exposure to UV (365 nm wavelength), according to the manufacturer’s instructions. .. The beads were washed three times with lysis buffer and analysed by immunoblotting with an anti-Myc antibody (Roche, USA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher ez link psoralen peg3 biotin bpso
    Ez Link Psoralen Peg3 Biotin Bpso, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ez link psoralen peg3 biotin bpso/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ez link psoralen peg3 biotin bpso - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results