ez link psoralen peg3 biotin bpso  (Thermo Fisher)


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    Name:
    EZ Link Psoralen PEG3 Biotin
    Description:
    Thermo Scientific EZ Link Psoralen PEG3 Biotin enables biotinylation of DNA or RNA via UV light activated intercalation of the psoralen group with thymine and other pyrimidine containing bases to form a covalent bonds Features of EZ Link Psoralen PEG3 Biotin • Biomolecular labeling biotinylate DNA or RNA approximately 1 biotin per 10 to 20 bases without interfering with hybridization • Photo reactive covalent attachment occurs in the presence of UV light 350nm for 10 to 30 minutes • Psoralen activated reacts through a cyclo addition mechanism with the 5 6 double bond in thymine and other pyrimidine containing bases • Pegylated spacer arm contains a hydrophilic 3 unit polyethylene glycol PEG group • Enhances solubility pegylation imparts water solubility to the biotinylated molecule helping to prevent aggregation of biotinylated antibodies stored in solution • Long reach spacer arm total length added to target is 36 9 angstroms minimizing steric hindrance for binding interactions with streptavidin Psoralen PEG3 Biotin is a photoactivatable reagent for biotinylation of DNA or RNA Upon activation with UV Light 350nm the psoralen group reacts with thymine and other pyrimidine containing bases to form a covalent bond The hydrophilic polyethylene glycol PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule thus reducing aggregation of labeled proteins stored in solution The PEG spacer arm also gives this reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules We manufacture biotin reagents to ensure the highest possible overall product integrity consistency and performance for the intended research applications Applications • Selective labeling of cell surface proteins Ref 1 • Biotinylation of hyaluronan Ref 2 • Labeling of endotoxin for receptor binding studies Ref 3
    Catalog Number:
    29986
    Price:
    None
    Applications:
    Protein Biology|Protein Labeling|Protein Labeling & Crosslinking
    Category:
    Labeling Detection Products
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    Structured Review

    Thermo Fisher ez link psoralen peg3 biotin bpso
    Thermo Scientific EZ Link Psoralen PEG3 Biotin enables biotinylation of DNA or RNA via UV light activated intercalation of the psoralen group with thymine and other pyrimidine containing bases to form a covalent bonds Features of EZ Link Psoralen PEG3 Biotin • Biomolecular labeling biotinylate DNA or RNA approximately 1 biotin per 10 to 20 bases without interfering with hybridization • Photo reactive covalent attachment occurs in the presence of UV light 350nm for 10 to 30 minutes • Psoralen activated reacts through a cyclo addition mechanism with the 5 6 double bond in thymine and other pyrimidine containing bases • Pegylated spacer arm contains a hydrophilic 3 unit polyethylene glycol PEG group • Enhances solubility pegylation imparts water solubility to the biotinylated molecule helping to prevent aggregation of biotinylated antibodies stored in solution • Long reach spacer arm total length added to target is 36 9 angstroms minimizing steric hindrance for binding interactions with streptavidin Psoralen PEG3 Biotin is a photoactivatable reagent for biotinylation of DNA or RNA Upon activation with UV Light 350nm the psoralen group reacts with thymine and other pyrimidine containing bases to form a covalent bond The hydrophilic polyethylene glycol PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule thus reducing aggregation of labeled proteins stored in solution The PEG spacer arm also gives this reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules We manufacture biotin reagents to ensure the highest possible overall product integrity consistency and performance for the intended research applications Applications • Selective labeling of cell surface proteins Ref 1 • Biotinylation of hyaluronan Ref 2 • Labeling of endotoxin for receptor binding studies Ref 3
    https://www.bioz.com/result/ez link psoralen peg3 biotin bpso/product/Thermo Fisher
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ez link psoralen peg3 biotin bpso - by Bioz Stars, 2020-09
    95/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response
    Article Snippet: .. Reagents and antibodies Poly(I:C), LPS, R837, PGN, chloroquine (Invivogen); TNFα, IL-1β (R & D Systems); D-galactosamine (Sigma); GM-CSF (peproTech); ELISA kit for murine IFN-β, TNFα, IL-6 (Biolegend); dual-specific luciferase assay kit (Promega); EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); lysosome isolation kit (sigma); mouse monoclonal antibodies against Flag, β-actin (Sigma), HA (OriGene), phospho-IBα (Cell Signaling Technology), TLR3 (IMGENEX); rabbit monoclonal antibodies against TLR3, phospho-IRF3 (Cell Signaling Technology); rabbit polyclonal antibodies against IRF3 (Santa Cruz Biotechnology), TRIF (Cell Signaling Technology), TBK1 and phospho-TBK1(EPITOMICS), Flag (MBL); Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody, Alexa Fluor 647-conjugated goat anti-mouse IgG antibody (Invitrogen) were purchased from the indicated manufacturers. .. Mouse antisera against human Mex3B were raised against recombinant human Mex3B fragments containing aa236-517 and aa1-517, respectively.

    Article Title: ZFYVE1 negatively regulates MDA5- but not RIG-I-mediated innate antiviral response
    Article Snippet: .. Reagents, antibodies, cells and viruses Trizol (Takara Bio), SYBR Green (Bio-Rad), RNase I (Ambion), Flag antibody-conjugated beads (Bimake), dual-specific luciferase assay kit (Promega), polybrene (Millipore), poly(I:C)-HMW (Invivogen), poly(I:C)-LMW (Invivogen), 5’ppp-dsRNA (Invivogen), DNAase I, 3 × Flag peptide (Sigma), Z-link Psoralen-PEG3-Biotin (Thermo), type II collagenase (Worthington), first-strand cDNA synthesis kit (Fermentas), GammaBind G Plus-Sepharose (Amersham Biosciences), and ELISA kits for murine IFN-β and TNF (BioLegend); mouse monoclonal antibodies for Flag and β-actin (Sigma), HA (Origene), p-IRF3, p65 and p-p65 (Cell Signaling Technology), IRF3 (Santa Cruz Biotechnology), p-TBK1 and TBK1 (Abcam), and ZFYVE1 (ABclonal); Alexa Fluor 488-conjugated goat anti-mouse IgG and Alexa Fluor 594-conjugated goat anti-rabbit IgG (Invitrogen); and HEK293 (American Type Culture Collection) and THP1 cells (American type culture collection) were purchased from the indicated companies. ..

    Article Title: ZCCHC3 modulates TLR3-mediated signaling by promoting recruitment of TRIF to TLR3
    Article Snippet: .. Reagents, antibodies, and cells poly(I:C), R848, PGN, and Lipofectamine 2000 (InvivoGen); MPLA and LPS (Sigma); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); puromycin and EZ-link Psoralen–PEG3 –Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6, and TNFα (BioLegend). .. Mouse monoclonal antibodies against HA (Origene), FLAG, and β-actin, anti-HA-Peroxidase, and anti-FLAG-M2-Peroxidase (Sigma) and rabbit monoclonal antibodies against TLR3, phosphor-IRF3, p65 (Cell Signaling Technology), phosphor-TBK1, TBK1, TRIF (Abcam), and IRF3 (Santa Cruz Biotechnology) were purchased from the indicated manufacturers.

    Isolation:

    Article Title: The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response
    Article Snippet: .. Reagents and antibodies Poly(I:C), LPS, R837, PGN, chloroquine (Invivogen); TNFα, IL-1β (R & D Systems); D-galactosamine (Sigma); GM-CSF (peproTech); ELISA kit for murine IFN-β, TNFα, IL-6 (Biolegend); dual-specific luciferase assay kit (Promega); EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); lysosome isolation kit (sigma); mouse monoclonal antibodies against Flag, β-actin (Sigma), HA (OriGene), phospho-IBα (Cell Signaling Technology), TLR3 (IMGENEX); rabbit monoclonal antibodies against TLR3, phospho-IRF3 (Cell Signaling Technology); rabbit polyclonal antibodies against IRF3 (Santa Cruz Biotechnology), TRIF (Cell Signaling Technology), TBK1 and phospho-TBK1(EPITOMICS), Flag (MBL); Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody, Alexa Fluor 647-conjugated goat anti-mouse IgG antibody (Invitrogen) were purchased from the indicated manufacturers. .. Mouse antisera against human Mex3B were raised against recombinant human Mex3B fragments containing aa236-517 and aa1-517, respectively.

    SYBR Green Assay:

    Article Title: ZFYVE1 negatively regulates MDA5- but not RIG-I-mediated innate antiviral response
    Article Snippet: .. Reagents, antibodies, cells and viruses Trizol (Takara Bio), SYBR Green (Bio-Rad), RNase I (Ambion), Flag antibody-conjugated beads (Bimake), dual-specific luciferase assay kit (Promega), polybrene (Millipore), poly(I:C)-HMW (Invivogen), poly(I:C)-LMW (Invivogen), 5’ppp-dsRNA (Invivogen), DNAase I, 3 × Flag peptide (Sigma), Z-link Psoralen-PEG3-Biotin (Thermo), type II collagenase (Worthington), first-strand cDNA synthesis kit (Fermentas), GammaBind G Plus-Sepharose (Amersham Biosciences), and ELISA kits for murine IFN-β and TNF (BioLegend); mouse monoclonal antibodies for Flag and β-actin (Sigma), HA (Origene), p-IRF3, p65 and p-p65 (Cell Signaling Technology), IRF3 (Santa Cruz Biotechnology), p-TBK1 and TBK1 (Abcam), and ZFYVE1 (ABclonal); Alexa Fluor 488-conjugated goat anti-mouse IgG and Alexa Fluor 594-conjugated goat anti-rabbit IgG (Invitrogen); and HEK293 (American Type Culture Collection) and THP1 cells (American type culture collection) were purchased from the indicated companies. ..

    Luciferase:

    Article Title: The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response
    Article Snippet: .. Reagents and antibodies Poly(I:C), LPS, R837, PGN, chloroquine (Invivogen); TNFα, IL-1β (R & D Systems); D-galactosamine (Sigma); GM-CSF (peproTech); ELISA kit for murine IFN-β, TNFα, IL-6 (Biolegend); dual-specific luciferase assay kit (Promega); EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); lysosome isolation kit (sigma); mouse monoclonal antibodies against Flag, β-actin (Sigma), HA (OriGene), phospho-IBα (Cell Signaling Technology), TLR3 (IMGENEX); rabbit monoclonal antibodies against TLR3, phospho-IRF3 (Cell Signaling Technology); rabbit polyclonal antibodies against IRF3 (Santa Cruz Biotechnology), TRIF (Cell Signaling Technology), TBK1 and phospho-TBK1(EPITOMICS), Flag (MBL); Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody, Alexa Fluor 647-conjugated goat anti-mouse IgG antibody (Invitrogen) were purchased from the indicated manufacturers. .. Mouse antisera against human Mex3B were raised against recombinant human Mex3B fragments containing aa236-517 and aa1-517, respectively.

    Article Title: ZFYVE1 negatively regulates MDA5- but not RIG-I-mediated innate antiviral response
    Article Snippet: .. Reagents, antibodies, cells and viruses Trizol (Takara Bio), SYBR Green (Bio-Rad), RNase I (Ambion), Flag antibody-conjugated beads (Bimake), dual-specific luciferase assay kit (Promega), polybrene (Millipore), poly(I:C)-HMW (Invivogen), poly(I:C)-LMW (Invivogen), 5’ppp-dsRNA (Invivogen), DNAase I, 3 × Flag peptide (Sigma), Z-link Psoralen-PEG3-Biotin (Thermo), type II collagenase (Worthington), first-strand cDNA synthesis kit (Fermentas), GammaBind G Plus-Sepharose (Amersham Biosciences), and ELISA kits for murine IFN-β and TNF (BioLegend); mouse monoclonal antibodies for Flag and β-actin (Sigma), HA (Origene), p-IRF3, p65 and p-p65 (Cell Signaling Technology), IRF3 (Santa Cruz Biotechnology), p-TBK1 and TBK1 (Abcam), and ZFYVE1 (ABclonal); Alexa Fluor 488-conjugated goat anti-mouse IgG and Alexa Fluor 594-conjugated goat anti-rabbit IgG (Invitrogen); and HEK293 (American Type Culture Collection) and THP1 cells (American type culture collection) were purchased from the indicated companies. ..

    Article Title: ZCCHC3 modulates TLR3-mediated signaling by promoting recruitment of TRIF to TLR3
    Article Snippet: .. Reagents, antibodies, and cells poly(I:C), R848, PGN, and Lipofectamine 2000 (InvivoGen); MPLA and LPS (Sigma); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); puromycin and EZ-link Psoralen–PEG3 –Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6, and TNFα (BioLegend). .. Mouse monoclonal antibodies against HA (Origene), FLAG, and β-actin, anti-HA-Peroxidase, and anti-FLAG-M2-Peroxidase (Sigma) and rabbit monoclonal antibodies against TLR3, phosphor-IRF3, p65 (Cell Signaling Technology), phosphor-TBK1, TBK1, TRIF (Abcam), and IRF3 (Santa Cruz Biotechnology) were purchased from the indicated manufacturers.

    Purification:

    Article Title: Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts
    Article Snippet: .. 50–100 ng of purified) inserts were biotinylated using the EZ-Link™ Psoralen-PEG3-Biotin (ThermoFisher Scientific). .. Membranes were pre-hybridized at 55 °C in North2South hybridization buffer (Pierce) for 30 min followed by overnight hybridization with denatured biotinylated probes at 5 ng/ml in the same buffer at 55 °C.

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  • 95
    Thermo Fisher ez link psoralen peg3 biotin bpso
    Ez Link Psoralen Peg3 Biotin Bpso, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ez link psoralen peg3 biotin bpso/product/Thermo Fisher
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ez link psoralen peg3 biotin bpso - by Bioz Stars, 2020-09
    95/100 stars
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