extravidinr alkaline phosphatase  (Millipore)


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  • 99
    Name:
    ExtrAvidin
    Description:

    Catalog Number:
    e2511
    Price:
    None
    Applications:
    ExtrAvidin(R) may be used in conjunction with biotin in techniques such as ELISA, RIA, immunohistochemistry, protein blotting, and with DNA probes. It was used to capture biotinylated RNA in a study to understand translation initiation pathway using immobilised mRNA analogues. It was used to bind to biotinylated surface during preparation of substrates for protein assembly.
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    Millipore extravidinr alkaline phosphatase

    https://www.bioz.com/result/extravidinr alkaline phosphatase/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    extravidinr alkaline phosphatase - by Bioz Stars, 2020-09
    99/100 stars

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    Incubation:

    Article Title: The Genomic Sequences Bound to Special AT-rich Sequence-binding Protein 1 (SATB1) In Vivo in Jurkat T Cells Are Tightly Associated with the Nuclear Matrix at the Bases of the Chromatin Loops
    Article Snippet: .. Samples were incubated in 1:500 diluted FITC-conjugated ExtrAvidin (2.7 mg/ml) or rhodamine-conjugated ExtrAvidin (3.5 mg/ml) ( Sigma Chemical Co. ) in 4× SSC, 1% BSA for 30 min at room temperature. .. Samples were then rinsed for 10 min each in 4× SSC, 4× SSC/0.1% Triton X-100, then 4× SSC.

    Article Title: Proteomic examination of Leishmania chagasi plasma membrane proteins: contrast between avirulent and virulent (metacyclic) parasite forms
    Article Snippet: .. After blocking in 10% goat serum/PBS, cells were incubated in 1:200 diluted FITC-conjugated extravidin (Sigma) in blocking solution. .. The cells were suspended in VECTASHIELD® mounting medium (Vector Laboratories, Burlingame, CA) and examined using a Zeiss 510 confocal microscope (Thornwood, NY).

    Article Title: Targeting peptide nucleic acid (PNA) oligomers to mitochondria within cells by conjugation to lipophilic cations: implications for mitochondrial DNA replication, expression and disease
    Article Snippet: .. After washes in PBS (3 × 5 min) and 1% BSA/PBS, sections were incubated with colloidal gold particles (10 nm) linked to either goat anti-rabbit IgG (1:5 in 1% Tween-20/PBS, Sigma) or extravidin (1:10 in 1% Tween-20/PBS, Sigma) for 1 h, followed by washing and fixation (2% glutaraldehyde for 15 min). .. The sections were then stained with osmium tetroxide/uranyl acetate/lead citrate and examined in a CM100 transmission electron microscope.

    Binding Assay:

    Article Title: High-affinity Anticalins with aggregation-blocking activity directed against the Alzheimer β-amyloid peptide
    Article Snippet: .. Dilution series of biotinylated Aβ targets, or control proteins, were added and binding was detected with an ExtrAvidin/AP conjugate (Sigma–Aldrich), followed by chromogenic reaction. .. Kinetic affinity data were measured on Biacore T100 (Anticalins H1GA and US7 with immobilized Aβ40) and Biacore X (immobilized MBP-Aβ40) instruments (Biacore) using PBS supplemented with 0.005% (v/v) Surfactant P20 or Tween 20 as running buffer.

    Blocking Assay:

    Article Title: Antiviral activity of recombinant ankyrin targeted to the capsid domain of HIV-1 Gag polyprotein
    Article Snippet: .. 10 μmol biotinylated proteins was spotted on nitrocellulose membrane, membrane blocked with blocking buffer (5% BSA in TBS), and biotin groups revealed by extravidin-HRP (Sigma) used at dilution 1:5, 000 in blocking buffer (1 h at RT with shaking) and BM Blue POD Substrate (Roche Diagnostics GmbH). .. Assessment of ankyrin reactivity towards HIV-1 MA-CA polyprotein (i) Competitive ELISA .

    Article Title: Proteomic examination of Leishmania chagasi plasma membrane proteins: contrast between avirulent and virulent (metacyclic) parasite forms
    Article Snippet: .. After blocking in 10% goat serum/PBS, cells were incubated in 1:200 diluted FITC-conjugated extravidin (Sigma) in blocking solution. .. The cells were suspended in VECTASHIELD® mounting medium (Vector Laboratories, Burlingame, CA) and examined using a Zeiss 510 confocal microscope (Thornwood, NY).

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  • 99
    Millipore rabbit extravidin alkaline phosphatase staining kit antibody
    Rabbit Extravidin Alkaline Phosphatase Staining Kit Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit extravidin alkaline phosphatase staining kit antibody/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit extravidin alkaline phosphatase staining kit antibody - by Bioz Stars, 2020-09
    99/100 stars
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