extravidine alkaline phosphatase  (Millipore)


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  • 99
    Name:
    Alkaline Phosphatase
    Description:
    Orthophosphoric monoester phosphohydrolase alkaline optimum Amino groups 8 16 moles mole enzyme
    Catalog Number:
    10567752001
    Price:
    None
    Applications:
    Alkaline phosphatase can be coupled to other proteins via its amino groups. The enzyme solution can be used directly for conjugation without prior dialysis.
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    Structured Review

    Millipore extravidine alkaline phosphatase
    Orthophosphoric monoester phosphohydrolase alkaline optimum Amino groups 8 16 moles mole enzyme
    https://www.bioz.com/result/extravidine alkaline phosphatase/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    extravidine alkaline phosphatase - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Cytidine Deaminase Motifs within the DYW Domain of Two Pentatricopeptide Repeat-containing Proteins Are Required for Site-specific Chloroplast RNA Editing *
    Article Snippet: .. The protein extracts from each set of transfected protoplasts, as well as protein extract from untransfected wild-type and qed1 protoplasts and along with bacteria alkaline phosphatase (BAP) tagged with FLAG (Sigma), were then probed with α-FLAG antibody to probe for expression of the N-tagged constructs ( ). .. As expected, no signal was observed in the wild-type and qed1 protein extracts from protoplasts that were not transfected by the FLAG-tagged constructs.

    AST Assay:

    Article Title: Hepatoprotective effect of food preservatives (butylated hydroxyanisole, butylated hydroxytoluene) on carbon tetrachloride-induced hepatotoxicity in rat
    Article Snippet: .. 2.3.1 Biochemical markers of hepatotoxicity Serum hepatic marker enzymes namely, serum glutamate oxaloacetate transaminases (AST), serum glutamate pyruvate transaminase (ALT) , alkaline phosphatase (ALP) , LDH , bilirubin , total protein concentration were measured by using assay kits Sigma (USA). ..

    Construct:

    Article Title: Cytidine Deaminase Motifs within the DYW Domain of Two Pentatricopeptide Repeat-containing Proteins Are Required for Site-specific Chloroplast RNA Editing *
    Article Snippet: .. The protein extracts from each set of transfected protoplasts, as well as protein extract from untransfected wild-type and qed1 protoplasts and along with bacteria alkaline phosphatase (BAP) tagged with FLAG (Sigma), were then probed with α-FLAG antibody to probe for expression of the N-tagged constructs ( ). .. As expected, no signal was observed in the wild-type and qed1 protein extracts from protoplasts that were not transfected by the FLAG-tagged constructs.

    ALP Assay:

    Article Title: Hepatoprotective effect of food preservatives (butylated hydroxyanisole, butylated hydroxytoluene) on carbon tetrachloride-induced hepatotoxicity in rat
    Article Snippet: .. 2.3.1 Biochemical markers of hepatotoxicity Serum hepatic marker enzymes namely, serum glutamate oxaloacetate transaminases (AST), serum glutamate pyruvate transaminase (ALT) , alkaline phosphatase (ALP) , LDH , bilirubin , total protein concentration were measured by using assay kits Sigma (USA). ..

    Article Title: The influence of estrogen deficiency on bone around osseointegrated dental implants. An experimental study in the rat jaw model
    Article Snippet: .. Tartrate-resistant acid phosphate (TRAP) and alkaline phosphatase (ALP) activities were also measured in serum by biochemical assay using the α-naphthyl- phosphate (Sigma Aldrich, Saint Louis, MO, EUA) as a substrate. .. After using D’Agostino test for evaluation of data normality, both analysis of Mann Whitney (used for OCN, CTX, OPG, RANKL, TRAP and ALP activity measurements) and Student’s t-test (for BMD of femur and lumbar vertebrae, BIC and BAFO analyses) were used for comparison of the groups.

    Activity Assay:

    Article Title: Functional and Bioinformatics Analysis of Two Campylobacter jejuni Homologs of the Thiol-Disulfide Oxidoreductase, DsbA
    Article Snippet: .. The PhoX (alkaline phosphatase) activity in C. jejuni strains was determined on defined medium as described by van Mourik et al. , and quantified by monitoring the release of p-nitrophenol from p-nitrophenyl phosphate (PNPP) (Sigma). .. Determination of the CjDsbA1 redox state The redox state of CjDsbA1 was visualized by alkylating the free cysteine residues using 4-acetamido-4′-maleimidylstilbene-2,2′-disulfonic acid (AMS), resulting in a molecular mass increase of 0.5 kDa.

    Expressing:

    Article Title: Cytidine Deaminase Motifs within the DYW Domain of Two Pentatricopeptide Repeat-containing Proteins Are Required for Site-specific Chloroplast RNA Editing *
    Article Snippet: .. The protein extracts from each set of transfected protoplasts, as well as protein extract from untransfected wild-type and qed1 protoplasts and along with bacteria alkaline phosphatase (BAP) tagged with FLAG (Sigma), were then probed with α-FLAG antibody to probe for expression of the N-tagged constructs ( ). .. As expected, no signal was observed in the wild-type and qed1 protein extracts from protoplasts that were not transfected by the FLAG-tagged constructs.

    Protein Concentration:

    Article Title: Hepatoprotective effect of food preservatives (butylated hydroxyanisole, butylated hydroxytoluene) on carbon tetrachloride-induced hepatotoxicity in rat
    Article Snippet: .. 2.3.1 Biochemical markers of hepatotoxicity Serum hepatic marker enzymes namely, serum glutamate oxaloacetate transaminases (AST), serum glutamate pyruvate transaminase (ALT) , alkaline phosphatase (ALP) , LDH , bilirubin , total protein concentration were measured by using assay kits Sigma (USA). ..

    Marker:

    Article Title: Hepatoprotective effect of food preservatives (butylated hydroxyanisole, butylated hydroxytoluene) on carbon tetrachloride-induced hepatotoxicity in rat
    Article Snippet: .. 2.3.1 Biochemical markers of hepatotoxicity Serum hepatic marker enzymes namely, serum glutamate oxaloacetate transaminases (AST), serum glutamate pyruvate transaminase (ALT) , alkaline phosphatase (ALP) , LDH , bilirubin , total protein concentration were measured by using assay kits Sigma (USA). ..

    Staining:

    Article Title: The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation
    Article Snippet: .. Alkaline Phosphatase Staining Alkaline phosphatase was purchased from Millipore (SCR004). ..

    Article Title: High throughput sequencing identifies an imprinted gene, Grb10, associated with the pluripotency state in nuclear transfer embryonic stem cells
    Article Snippet: .. Alkaline phosphatase (AP) staining AP staining was performed with a Leukocyte Alkaline Phosphatase Kit (Sigma, St Louis, MO, USA) according to the manufacturer's instructions. .. Karyotype analysis The cells were incubated in ESCs medium with 0.25 μg/ml colcemid (Invitrogen, Thermo Fisher Scientific) for 2-3 h and harvested with 0.05% Trypsin-EDTA (Invitrogen, Thermo Fisher Scientific).

    Article Title: Biological Functions of miR-29b Contribute to Positive Regulation of Osteoblast Differentiation *
    Article Snippet: .. Cell layers were either harvested for protein and mRNA or fixed in 2% paraformaldehyde for histochemical detection of alkaline phosphatase (AlkP) (using Sigma reagents) and mineral by the von Kossa stain (3% silver nitrate in H2 0) as previously described ( , ). .. Whole cell lysate preparation and Western blot analysis were performed as described previously ( ).

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  • 99
    Millipore rabbit extravidin alkaline phosphatase staining kit antibody
    Rabbit Extravidin Alkaline Phosphatase Staining Kit Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit extravidin alkaline phosphatase staining kit antibody/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit extravidin alkaline phosphatase staining kit antibody - by Bioz Stars, 2020-09
    99/100 stars
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