extravidin alkaline phosphatase  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    ExtrAvidin Alkaline Phosphatase
    Description:
    Avidin is a tetrameric or dimeric biotin binding protein produced in the oviducts of birds reptiles and amphibians and deposited in the whites of their eggs It consists of four high affinity binding sites for biotin ExtrAvidin R is prepared from egg white avidin It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin It is a biotin binding protein produced by the bacteria Streptomyces avidinii ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques
    Catalog Number:
    e2636
    Price:
    None
    Applications:
    ExtrAvidin(TM) -Alkaline Phosphatase has been used in:. Dot blot. Immunohistochemistry (formalin-fixed, paraffin-embedded sections) . Indirect ELISA . Enzyme linked immunosorbent assay (ELISA). Enzyme-linked immunosorbent spot (ELISPOT) analysis. Competitive ELISA
    Buy from Supplier


    Structured Review

    Millipore extravidin alkaline phosphatase
    ExtrAvidin Alkaline Phosphatase
    Avidin is a tetrameric or dimeric biotin binding protein produced in the oviducts of birds reptiles and amphibians and deposited in the whites of their eggs It consists of four high affinity binding sites for biotin ExtrAvidin R is prepared from egg white avidin It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin It is a biotin binding protein produced by the bacteria Streptomyces avidinii ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques
    https://www.bioz.com/result/extravidin alkaline phosphatase/product/Millipore
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    extravidin alkaline phosphatase - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "TSG-6 inhibits neutrophil migration via direct interaction with the chemokine CXCL8"

    Article Title: TSG-6 inhibits neutrophil migration via direct interaction with the chemokine CXCL8

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1300194

    Interaction between the Link module domain of T6G-6 and the GAG-binding site of CXCL8 inhibits CXCL8-heparin binding and CXCL8-mediated neutrophil transmigration WT or mutant CXCL8 (250 nM) was immobilized onto MaxiSorp plates and b-heparin (0-100 ng/well) (A) or b-heparin (25 ng/well) in combination with a range of concentrations of rhTSG-6 or Link_TSG6 (0-1000 nM) (B) , or in combination with a range of concentrations of WT Link_TSG6, Link_TSG6_D or Link_TSG6_T (0-2000 nM) (C) was added in the fluid phase. Binding of b-heparin was detected using Extravidin alkaline phosphatase followed by disodium p -nitrophenyl phosphate; absorbance at 405 nm was determined after 5 min (A) or 10 min (B,C) . Data are plotted as mean values ( n = 8) ± SEM and fitted using Origin Pro (v8). K D values of 5 ± 12 nM and 26 ± 11 nM were determined for the binding of b-heparin to WT CXCL8 and CXCL8_D, respectively (A) . IC 50 values of ( B ) 81 ± 18 nM and 71 ± 14 nM for the inhibition of b-heparin binding to CXCL8 by rhTSG-6 and Link_TSG6, respectively, and (C) 105 ± 5 nM, 172 ± 3 nM and 45 ± 5 nM for the inhibition of this interaction by WT Link_TSG6, Link_TSG6_D and Link_TSG6_T, respectively, were determined. In (D) , migration of differentiated HL-60 cells across an EA.hy 926 cell monolayer was determined in response to CXCL8 (3.6 nM (+)) alone or in combination with 5:1 molar ratios of WT Link_TSG6, Link_TSG6_D or Link_TSG6_T ( n = 8). Data are plotted as mean values (± SEM) relative to non-stimulated controls (−), where ** and *** = p
    Figure Legend Snippet: Interaction between the Link module domain of T6G-6 and the GAG-binding site of CXCL8 inhibits CXCL8-heparin binding and CXCL8-mediated neutrophil transmigration WT or mutant CXCL8 (250 nM) was immobilized onto MaxiSorp plates and b-heparin (0-100 ng/well) (A) or b-heparin (25 ng/well) in combination with a range of concentrations of rhTSG-6 or Link_TSG6 (0-1000 nM) (B) , or in combination with a range of concentrations of WT Link_TSG6, Link_TSG6_D or Link_TSG6_T (0-2000 nM) (C) was added in the fluid phase. Binding of b-heparin was detected using Extravidin alkaline phosphatase followed by disodium p -nitrophenyl phosphate; absorbance at 405 nm was determined after 5 min (A) or 10 min (B,C) . Data are plotted as mean values ( n = 8) ± SEM and fitted using Origin Pro (v8). K D values of 5 ± 12 nM and 26 ± 11 nM were determined for the binding of b-heparin to WT CXCL8 and CXCL8_D, respectively (A) . IC 50 values of ( B ) 81 ± 18 nM and 71 ± 14 nM for the inhibition of b-heparin binding to CXCL8 by rhTSG-6 and Link_TSG6, respectively, and (C) 105 ± 5 nM, 172 ± 3 nM and 45 ± 5 nM for the inhibition of this interaction by WT Link_TSG6, Link_TSG6_D and Link_TSG6_T, respectively, were determined. In (D) , migration of differentiated HL-60 cells across an EA.hy 926 cell monolayer was determined in response to CXCL8 (3.6 nM (+)) alone or in combination with 5:1 molar ratios of WT Link_TSG6, Link_TSG6_D or Link_TSG6_T ( n = 8). Data are plotted as mean values (± SEM) relative to non-stimulated controls (−), where ** and *** = p

    Techniques Used: Binding Assay, Transmigration Assay, Mutagenesis, Inhibition, Migration

    2) Product Images from "Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting"

    Article Title: Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting

    Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

    doi: 10.5114/ada.2017.65623

    A – IgE immunoblot from D. glomerata 2-D separation, revealed with biotin-ExtrAvidin-alkaline phosphatase anti-dog-IgE. Dog D. glomerata allergogram with a pool of sera. B – Two-dimensional diagram from dog D. glomerata allergogram in Figure 3 A. Recognized allergen spots are identified from a to n
    Figure Legend Snippet: A – IgE immunoblot from D. glomerata 2-D separation, revealed with biotin-ExtrAvidin-alkaline phosphatase anti-dog-IgE. Dog D. glomerata allergogram with a pool of sera. B – Two-dimensional diagram from dog D. glomerata allergogram in Figure 3 A. Recognized allergen spots are identified from a to n

    Techniques Used:

    A – IgE immunoblot from P. pratense 2-D separation, revealed with biotin-ExtrAvidin-alkaline phosphatase antidog-IgE. Dog P. pratense allergogram with a pool of sera. B – Two-dimensional diagram from dog P. pratense allergogram in Figure 4 A. Recognized allergen spots are identified from a to z 7
    Figure Legend Snippet: A – IgE immunoblot from P. pratense 2-D separation, revealed with biotin-ExtrAvidin-alkaline phosphatase antidog-IgE. Dog P. pratense allergogram with a pool of sera. B – Two-dimensional diagram from dog P. pratense allergogram in Figure 4 A. Recognized allergen spots are identified from a to z 7

    Techniques Used:

    3) Product Images from "TSG-6 inhibits neutrophil migration via direct interaction with the chemokine CXCL8"

    Article Title: TSG-6 inhibits neutrophil migration via direct interaction with the chemokine CXCL8

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1300194

    Interaction between the Link module domain of T6G-6 and the GAG-binding site of CXCL8 inhibits CXCL8-heparin binding and CXCL8-mediated neutrophil transmigration WT or mutant CXCL8 (250 nM) was immobilized onto MaxiSorp plates and b-heparin (0-100 ng/well) (A) or b-heparin (25 ng/well) in combination with a range of concentrations of rhTSG-6 or Link_TSG6 (0-1000 nM) (B) , or in combination with a range of concentrations of WT Link_TSG6, Link_TSG6_D or Link_TSG6_T (0-2000 nM) (C) was added in the fluid phase. Binding of b-heparin was detected using Extravidin alkaline phosphatase followed by disodium p -nitrophenyl phosphate; absorbance at 405 nm was determined after 5 min (A) or 10 min (B,C) . Data are plotted as mean values ( n = 8) ± SEM and fitted using Origin Pro (v8). K D values of 5 ± 12 nM and 26 ± 11 nM were determined for the binding of b-heparin to WT CXCL8 and CXCL8_D, respectively (A) . IC 50 values of ( B ) 81 ± 18 nM and 71 ± 14 nM for the inhibition of b-heparin binding to CXCL8 by rhTSG-6 and Link_TSG6, respectively, and (C) 105 ± 5 nM, 172 ± 3 nM and 45 ± 5 nM for the inhibition of this interaction by WT Link_TSG6, Link_TSG6_D and Link_TSG6_T, respectively, were determined. In (D) , migration of differentiated HL-60 cells across an EA.hy 926 cell monolayer was determined in response to CXCL8 (3.6 nM (+)) alone or in combination with 5:1 molar ratios of WT Link_TSG6, Link_TSG6_D or Link_TSG6_T ( n = 8). Data are plotted as mean values (± SEM) relative to non-stimulated controls (−), where ** and *** = p
    Figure Legend Snippet: Interaction between the Link module domain of T6G-6 and the GAG-binding site of CXCL8 inhibits CXCL8-heparin binding and CXCL8-mediated neutrophil transmigration WT or mutant CXCL8 (250 nM) was immobilized onto MaxiSorp plates and b-heparin (0-100 ng/well) (A) or b-heparin (25 ng/well) in combination with a range of concentrations of rhTSG-6 or Link_TSG6 (0-1000 nM) (B) , or in combination with a range of concentrations of WT Link_TSG6, Link_TSG6_D or Link_TSG6_T (0-2000 nM) (C) was added in the fluid phase. Binding of b-heparin was detected using Extravidin alkaline phosphatase followed by disodium p -nitrophenyl phosphate; absorbance at 405 nm was determined after 5 min (A) or 10 min (B,C) . Data are plotted as mean values ( n = 8) ± SEM and fitted using Origin Pro (v8). K D values of 5 ± 12 nM and 26 ± 11 nM were determined for the binding of b-heparin to WT CXCL8 and CXCL8_D, respectively (A) . IC 50 values of ( B ) 81 ± 18 nM and 71 ± 14 nM for the inhibition of b-heparin binding to CXCL8 by rhTSG-6 and Link_TSG6, respectively, and (C) 105 ± 5 nM, 172 ± 3 nM and 45 ± 5 nM for the inhibition of this interaction by WT Link_TSG6, Link_TSG6_D and Link_TSG6_T, respectively, were determined. In (D) , migration of differentiated HL-60 cells across an EA.hy 926 cell monolayer was determined in response to CXCL8 (3.6 nM (+)) alone or in combination with 5:1 molar ratios of WT Link_TSG6, Link_TSG6_D or Link_TSG6_T ( n = 8). Data are plotted as mean values (± SEM) relative to non-stimulated controls (−), where ** and *** = p

    Techniques Used: Binding Assay, Transmigration Assay, Mutagenesis, Inhibition, Migration

    Related Articles

    Protease Inhibitor:

    Article Title: Immunosuppressive Drugs Affect High-Mannose/Hybrid N-Glycans on Human Allostimulated Leukocytes
    Article Snippet: .. Rabbit polyclonal anti-GAPDH (G9545), AP-conjugated goat anti-mouse (084K4861) antibody, AP-conjugated ExtrAvidin (E2636), FITC-conjugated ExtrAvidin (E2761), Coomassie Brilliant Blue G (B2025), protease inhibitor cocktail (P2714), and trypan blue (T8154) were purchased from Sigma-Aldrich. .. Horseradish peroxidase- (HRP-) conjugated horse anti-mouse (7076S) and sheep anti-rabbit (70742) antibody were obtained from Cell Signaling Technology.

    Incubation:

    Article Title: Isolation of lactic acid bacteria bound to the porcine intestinal mucosa and an analysis of their moonlighting adhesins
    Article Snippet: .. After washing with TBS-T, ExtrAvidin Alkaline Phosphatase Conjugate (Sigma-Aldrich) was added, followed by incubation for 30 min. After washing with TBS-T, ECF Substrate for Western Blotting (GE Healthcare, Tokyo, Japan) was added, and visualization was performed using an LAS-3000 luminescent image analyzer (Fujifilm, Tokyo, Japan) (excitation, 440 nm; emission, 560 nm). .. Biotinylated molecular weight markers (Sigma-Aldrich) were used for SDS-PAGE.

    Article Title: Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting
    Article Snippet: .. Specific IgE was detected by anti-dog-IgE biotin-labeled monoclonal antibody (AbD Serotec, Kidlington, UK) at 1 : 500 in blocking buffer, followed by 4 × 5 min washes in 0.9% NaCl – 0.1% Tween-20 and 1 h incubation with ExtrAvidin-alkaline phosphatase (Sigma-Aldrich) at 1 : 5000. .. Another 4 × 5 min washing cycle was done and specific IgE visualization was performed by chromogenic reaction with nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (Sigma-Aldrich) in substrate buffer.

    Western Blot:

    Article Title: Isolation of lactic acid bacteria bound to the porcine intestinal mucosa and an analysis of their moonlighting adhesins
    Article Snippet: .. After washing with TBS-T, ExtrAvidin Alkaline Phosphatase Conjugate (Sigma-Aldrich) was added, followed by incubation for 30 min. After washing with TBS-T, ECF Substrate for Western Blotting (GE Healthcare, Tokyo, Japan) was added, and visualization was performed using an LAS-3000 luminescent image analyzer (Fujifilm, Tokyo, Japan) (excitation, 440 nm; emission, 560 nm). .. Biotinylated molecular weight markers (Sigma-Aldrich) were used for SDS-PAGE.

    Blocking Assay:

    Article Title: Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting
    Article Snippet: .. Specific IgE was detected by anti-dog-IgE biotin-labeled monoclonal antibody (AbD Serotec, Kidlington, UK) at 1 : 500 in blocking buffer, followed by 4 × 5 min washes in 0.9% NaCl – 0.1% Tween-20 and 1 h incubation with ExtrAvidin-alkaline phosphatase (Sigma-Aldrich) at 1 : 5000. .. Another 4 × 5 min washing cycle was done and specific IgE visualization was performed by chromogenic reaction with nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (Sigma-Aldrich) in substrate buffer.

    MTT Assay:

    Article Title: Prokaryotic soluble overexpression and purification of oncostatin M using a fusion approach and genetically engineered E. coli strains
    Article Snippet: .. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT), ExtrAvidin-alkaline phosphatase, phorbol 12-myristate 13-acetate (PMA), 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), and ionomycin were from Sigma Aldrich (St. Louis, MO). .. Commercial OSM was purchased from R & D Systems (Minneapolis, MN).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore rabbit extravidin alkaline phosphatase staining kit antibody
    Rabbit Extravidin Alkaline Phosphatase Staining Kit Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit extravidin alkaline phosphatase staining kit antibody/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit extravidin alkaline phosphatase staining kit antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results