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    Structured Review

    Qiagen extraction kit
    Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/extraction kit/product/Qiagen
    Average 99 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    extraction kit - by Bioz Stars, 2020-03
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    Diagnostic Assay:

    Article Title: Responding to ever-changing epidemiological dynamics of Ebola virus disease
    Article Snippet: .. Recently, RT-LAMP in combination with a new extraction kit, SpeedXtract (Qiagen, Germany), and an improved diagnostic reverse transcription recombinase polymerase amplification assay (EBOV-RT-RPA), has been validated on sera and swabs in Guinea for case management as well as for safe burial management. .. The result on this combined device showed higher sensitivity than the RealStar Filovirus rRT-PCR kit (Altona-Diagnostics, Germany), and provided significantly fast results, within 30–60 min, with comparatively high specificity and sensitivity to the results shown by the above RT-LAMP method.

    Amplification:

    Article Title: Characterization of microflora in Latin-style cheeses by next-generation sequencing technology
    Article Snippet: Efforts exist to manipulate sample preparation to detect only cells with intact membranes by sample treatment with propidium monoazide in combination with PCR amplification [ ] or the generation of transcriptomes. .. First, the extraction kit used to perform the DNA extractions was chosen based on data collected in which the Qiagen DNeasy Blood and Tissue kit was compared to five other commercially-available kits for the extraction of Brucella neotomae DNA from the same Latin-style cheeses used in this study (T. Lusk, E. Strain, and J.A.

    Article Title: 1,4‐Dioxane‐degrading consortia can be enriched from uncontaminated soils: prevalence of Mycobacterium and soluble di‐iron monooxygenase genes
    Article Snippet: The extraction kit was the UltraClean® Microbial DNA Isolation Kit (MO BIO, Carlsbad, CA, USA). .. The V4 region of the 16S rRNA gene was amplified by PCR using the forward 515F and reverse 806R primers (Caporaso et al ., ).

    Article Title: Ancient DNA from mastics solidifies connection between material culture and genetics of mesolithic hunter–gatherers in Scandinavia
    Article Snippet: For the ble008 sample we tested one more extraction method using an extraction kit designed to process samples with high-inhibitor content (QIAamp PowerFecal DNA Kit, Qiagen), with slight modification to the protocol provided with the kit (described in the Methods chapter). .. The libraries were amplified using polymerase chain reaction (PCR) and shotgun sequenced at the Science for Life laboratories (SciLife) in Stockholm, on Illumina Hiseq X platform.

    Quantitative RT-PCR:

    Article Title: Responding to ever-changing epidemiological dynamics of Ebola virus disease
    Article Snippet: If rRT-PCR is not available for confirmatory diagnosis of EVD, RT-LAMP can be alternatively deployed for efficient decision-making and reduction of the health resource burden. .. Recently, RT-LAMP in combination with a new extraction kit, SpeedXtract (Qiagen, Germany), and an improved diagnostic reverse transcription recombinase polymerase amplification assay (EBOV-RT-RPA), has been validated on sera and swabs in Guinea for case management as well as for safe burial management.

    Real-time Polymerase Chain Reaction:

    Article Title: PSIII-25 Adipose gene expression of mature neutered male cats during the transition from a lean to an obese phenotype
    Article Snippet: .. RNA was isolated using a commercially available extraction kit specific for lipids (RNeasy lipid tissue mini kit, Qiagen). mRNA expression of over 40 genes encoding adipokines, transcription factors, glucose transporters, inflammatory processes, and metabolic enzymes was measured by Fluidigm multiplex qPCR. .. This generated a gene expression profile portraying the upregulation of genes associated with energy storage but not energy mobilization.

    Article Title: Glutamate signaling through the NMDA receptor reduces the expression of scleraxis in plantaris tendon derived cells
    Article Snippet: .. RNA isolation, reverse transcription, qPCR For the extraction of RNA, an extraction kit was used (code: 74106; Qiagen) and the protocol from the manufactures was followed. .. The isolated RNA was then reverse transcribed into cDNA via a High Capacity cDNA Reverse Transcription kit (code: 4368813; Applied Biosystems).

    Incubation:

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
    Article Snippet: These kits differ in the recommended time and temperature of tissue lysis with the PicoPure lysis incubation being 30 min at 42°C and the QIAGEN lysis incubation conditions being 5 min at room temperature. .. This was true when using either extraction kit, however, we note that our PixCell PicoPure samples consisted solely of samples captured from brain tissue and our CellCut QIAGEN samples were entirely from liver tissue (See Table ).

    Article Title: A Metagenomic-Based Approach for the Characterization of Bacterial Diversity Associated with Spontaneous Malolactic Fermentations in Wine
    Article Snippet: Genomic DNA Extraction Samples of 5 mL from the wine fermentations, incubated during 30 days, were centrifuged (10000 rpm, 5 min), washed with ultrapure water (1 vol), then with 1 vol of 10% TEN buffer (0.1M Tris-HCl pH 7.5, 0.05 M EDTA, 0.8 M NaCl) supplemented with polyvidone 10 (PVP) (2% w /v ) (Sigma-Aldrich, Saint Louis, MO, USA), and then twice with ultrapure water (1 vol). .. The genomic DNA from the 5 mL sample, after the washing steps, was extracted with the commercial extraction kit, known as PowerSoil Microbial DNA Isolation Kit (Mo Bio, Carlsbad, CA, USA), according to the manufacturer’s instructions.

    Expressing:

    Article Title: PSIII-25 Adipose gene expression of mature neutered male cats during the transition from a lean to an obese phenotype
    Article Snippet: .. RNA was isolated using a commercially available extraction kit specific for lipids (RNeasy lipid tissue mini kit, Qiagen). mRNA expression of over 40 genes encoding adipokines, transcription factors, glucose transporters, inflammatory processes, and metabolic enzymes was measured by Fluidigm multiplex qPCR. .. This generated a gene expression profile portraying the upregulation of genes associated with energy storage but not energy mobilization.

    Article Title: Exacerbation of Autoimmune Neuro-Inflammation in Mice Cured from Blood-Stage Plasmodium berghei Infection
    Article Snippet: RNA extraction from DP-T cells was carried our immediately after sorting, using an extraction kit according to the manufactureŕs instruction (RNeasy Micro kit, Qiagen USA). .. Expression of AIRE (Mm00477461_m1), IL-7 (Mm01295803_m1), IL-6 (Mm00446190_m1), IL-17 (Mm00439618_m1), IFNg (Mm01168134_m1), FOXP3 (Mm00475162_m1) and RAR-related orphan receptor C (RORc) (Mm01261022_m1) were analyzed in comparison to GAPDH (Mm99999915_g1, housekeeping gene) levels.

    Modification:

    Article Title: Ancient DNA from mastics solidifies connection between material culture and genetics of mesolithic hunter–gatherers in Scandinavia
    Article Snippet: .. For the ble008 sample we tested one more extraction method using an extraction kit designed to process samples with high-inhibitor content (QIAamp PowerFecal DNA Kit, Qiagen), with slight modification to the protocol provided with the kit (described in the Methods chapter). ..

    Recombinase Polymerase Amplification:

    Article Title: Responding to ever-changing epidemiological dynamics of Ebola virus disease
    Article Snippet: .. Recently, RT-LAMP in combination with a new extraction kit, SpeedXtract (Qiagen, Germany), and an improved diagnostic reverse transcription recombinase polymerase amplification assay (EBOV-RT-RPA), has been validated on sera and swabs in Guinea for case management as well as for safe burial management. .. The result on this combined device showed higher sensitivity than the RealStar Filovirus rRT-PCR kit (Altona-Diagnostics, Germany), and provided significantly fast results, within 30–60 min, with comparatively high specificity and sensitivity to the results shown by the above RT-LAMP method.

    Flow Cytometry:

    Article Title: Akonni TruTip® and Qiagen® Methods for Extraction of Fetal Circulating DNA - Evaluation by Real-Time and Digital PCR
    Article Snippet: At the time of this study, the only commercially available extraction kit for the isolation of cell-free DNA able to accommodate volumes of at least 5 mL was the manual Qiagen Circulating Nucleic Acid Kit, which uses silica filter spin columns [ ]. .. The binding matrix has greater porosity than a typical silica filter, which effectively lowers the backpressure, allowing bidirectional flow and processing of viscous samples such as plasma or blood without the high force of a vacuum manifold or centrifuge.

    Transferring:

    Article Title: Akonni TruTip® and Qiagen® Methods for Extraction of Fetal Circulating DNA - Evaluation by Real-Time and Digital PCR
    Article Snippet: At the time of this study, the only commercially available extraction kit for the isolation of cell-free DNA able to accommodate volumes of at least 5 mL was the manual Qiagen Circulating Nucleic Acid Kit, which uses silica filter spin columns [ ]. .. TruTip consists of a porous rigid silica monolith inserted into a pipette tip that connects to standard laboratory pipettors or automated liquid handling systems.

    Infection:

    Article Title: Responding to ever-changing epidemiological dynamics of Ebola virus disease
    Article Snippet: Recently, RT-LAMP in combination with a new extraction kit, SpeedXtract (Qiagen, Germany), and an improved diagnostic reverse transcription recombinase polymerase amplification assay (EBOV-RT-RPA), has been validated on sera and swabs in Guinea for case management as well as for safe burial management. .. A point-of-care diagnostic system such as RT-LAMP is essential to precisely screen and confirm each infection risk and case management in local settings.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Functional Polymorphism of the CK2? Intronless Gene Plays Oncogenic Roles in Lung Cancer
    Article Snippet: .. Semi-quantitative reverse transcription-PCR (RT-PCR) analysis Total RNA from cell lines and tissues was isolated using an extraction kit, and DNA was eliminated by on-column treatment with DNase (RNeasy Mini kit; Qiagen, Valencia, CA, USA). .. Semiquantitative RT-PCR was performed by using SuperScript One-step RT-PCR with Platinum Taq kit (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol.

    Article Title: Exacerbation of Autoimmune Neuro-Inflammation in Mice Cured from Blood-Stage Plasmodium berghei Infection
    Article Snippet: Paragraph title: RT-PCR assays ... RNA extraction from DP-T cells was carried our immediately after sorting, using an extraction kit according to the manufactureŕs instruction (RNeasy Micro kit, Qiagen USA).

    Generated:

    Article Title: PSIII-25 Adipose gene expression of mature neutered male cats during the transition from a lean to an obese phenotype
    Article Snippet: RNA was isolated using a commercially available extraction kit specific for lipids (RNeasy lipid tissue mini kit, Qiagen). mRNA expression of over 40 genes encoding adipokines, transcription factors, glucose transporters, inflammatory processes, and metabolic enzymes was measured by Fluidigm multiplex qPCR. .. This generated a gene expression profile portraying the upregulation of genes associated with energy storage but not energy mobilization.

    other:

    Article Title: Syndrome Evaluation System for Simultaneous Detection Pathogens Causing Acute Encephalitic Syndrome in India, Part-1: Development and Standardization of the Assay
    Article Snippet: The extraction kit used for RNA viruses was the Qiagen QIAamp Viral RNA Mini kit (Cat No. 52906) while that used for DNA organisms was the Qiagen QIAamp DNA mini kit (Cat no: 51306).

    Transmission Assay:

    Article Title: Responding to ever-changing epidemiological dynamics of Ebola virus disease
    Article Snippet: Recently, RT-LAMP in combination with a new extraction kit, SpeedXtract (Qiagen, Germany), and an improved diagnostic reverse transcription recombinase polymerase amplification assay (EBOV-RT-RPA), has been validated on sera and swabs in Guinea for case management as well as for safe burial management. .. Moreover, when combined with PEP therapy discussed in more detail below, RT-LAMP can support treatment-for-prevention measures and follow-up intervention for those at risk of sexual transmission.

    Polymerase Chain Reaction:

    Article Title: Characterization of microflora in Latin-style cheeses by next-generation sequencing technology
    Article Snippet: Efforts exist to manipulate sample preparation to detect only cells with intact membranes by sample treatment with propidium monoazide in combination with PCR amplification [ ] or the generation of transcriptomes. .. First, the extraction kit used to perform the DNA extractions was chosen based on data collected in which the Qiagen DNeasy Blood and Tissue kit was compared to five other commercially-available kits for the extraction of Brucella neotomae DNA from the same Latin-style cheeses used in this study (T. Lusk, E. Strain, and J.A.

    Article Title: Glutamate signaling through the NMDA receptor reduces the expression of scleraxis in plantaris tendon derived cells
    Article Snippet: RNA isolation, reverse transcription, qPCR For the extraction of RNA, an extraction kit was used (code: 74106; Qiagen) and the protocol from the manufactures was followed. .. The quantitative PCR (qPCR) was accomplished using TaqMan fast universal PCR mastermix (code: 4352042) and probes for Scx (code: Hs03054634; Applied Biosystems), Gls (code: Hs00248163; Applied Biosystems), and Got1 (code: Hs00157798; Applied Biosystems).

    Article Title: 1,4‐Dioxane‐degrading consortia can be enriched from uncontaminated soils: prevalence of Mycobacterium and soluble di‐iron monooxygenase genes
    Article Snippet: The extraction kit was the UltraClean® Microbial DNA Isolation Kit (MO BIO, Carlsbad, CA, USA). .. The V4 region of the 16S rRNA gene was amplified by PCR using the forward 515F and reverse 806R primers (Caporaso et al ., ).

    Article Title: Ancient DNA from mastics solidifies connection between material culture and genetics of mesolithic hunter–gatherers in Scandinavia
    Article Snippet: For the ble008 sample we tested one more extraction method using an extraction kit designed to process samples with high-inhibitor content (QIAamp PowerFecal DNA Kit, Qiagen), with slight modification to the protocol provided with the kit (described in the Methods chapter). .. The libraries were amplified using polymerase chain reaction (PCR) and shotgun sequenced at the Science for Life laboratories (SciLife) in Stockholm, on Illumina Hiseq X platform.

    Binding Assay:

    Article Title: Akonni TruTip® and Qiagen® Methods for Extraction of Fetal Circulating DNA - Evaluation by Real-Time and Digital PCR
    Article Snippet: At the time of this study, the only commercially available extraction kit for the isolation of cell-free DNA able to accommodate volumes of at least 5 mL was the manual Qiagen Circulating Nucleic Acid Kit, which uses silica filter spin columns [ ]. .. This method was compared to the newly developed technology from Akonni Biosystems, the TruTip® extraction method [ ] that also uses the BOOM® silica binding chemistry [ ] for nucleic acid binding and elution.

    DNA Extraction:

    Article Title: Characterization of microflora in Latin-style cheeses by next-generation sequencing technology
    Article Snippet: It is known that DNA extraction efficiency varies within and between laboratories, and that this can have an effect on subsequent microflora analysis [ ]. .. First, the extraction kit used to perform the DNA extractions was chosen based on data collected in which the Qiagen DNeasy Blood and Tissue kit was compared to five other commercially-available kits for the extraction of Brucella neotomae DNA from the same Latin-style cheeses used in this study (T. Lusk, E. Strain, and J.A.

    Article Title: A New Subspecies Identification and Population Study of the Asian Small-Clawed Otter (Aonyx cinereus) in Malay Peninsula and Southern Thailand Based on Fecal DNA Method
    Article Snippet: Paragraph title: 2.1. Sampling Locations, Samples, and DNA Isolation ... Total genomic DNA was extracted by using the standard extraction kit and the protocol provided by the Qiagen DNeasy Stool Kit [ ].

    Article Title: 1,4‐Dioxane‐degrading consortia can be enriched from uncontaminated soils: prevalence of Mycobacterium and soluble di‐iron monooxygenase genes
    Article Snippet: .. The extraction kit was the UltraClean® Microbial DNA Isolation Kit (MO BIO, Carlsbad, CA, USA). .. The V4 region of the 16S rRNA gene was amplified by PCR using the forward 515F and reverse 806R primers (Caporaso et al ., ).

    Article Title: Ancient DNA from mastics solidifies connection between material culture and genetics of mesolithic hunter–gatherers in Scandinavia
    Article Snippet: Extracting and authentication of aDNA from ancient mastics We performed DNA extraction on eight mastic samples from Huseby Klev (Fig. , Supplementary Note , Supplementary Data ). .. For the ble008 sample we tested one more extraction method using an extraction kit designed to process samples with high-inhibitor content (QIAamp PowerFecal DNA Kit, Qiagen), with slight modification to the protocol provided with the kit (described in the Methods chapter).

    Article Title: A Metagenomic-Based Approach for the Characterization of Bacterial Diversity Associated with Spontaneous Malolactic Fermentations in Wine
    Article Snippet: .. The genomic DNA from the 5 mL sample, after the washing steps, was extracted with the commercial extraction kit, known as PowerSoil Microbial DNA Isolation Kit (Mo Bio, Carlsbad, CA, USA), according to the manufacturer’s instructions. .. High Throughput Sequencing Illumina libraries were prepared using a NEXTflex 16S V4 Amplicon-Seq Kit (Bioo Scientific, Austin, TX, USA).

    RNA Sequencing Assay:

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
    Article Snippet: Type of Laser and RNA Extraction Method Impact RNA Quality In order to determine the optimal workflow for LCM RNA samples for downstream RNA-Seq we compared the RNA quality of LCM samples (as measured by RIN) captured from various mouse brain and liver samples (Table ) using two common LCM instruments, the MMI CellCut and Arcturus PixCell IIe, which differ in the type of laser used (UV and IR, respectively) and method of capturing tissue (polymer slides vs. polymer-coated caps, respectively). .. This was true when using either extraction kit, however, we note that our PixCell PicoPure samples consisted solely of samples captured from brain tissue and our CellCut QIAGEN samples were entirely from liver tissue (See Table ).

    Isolation:

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
    Article Snippet: Furthermore, for a within sample experiment to compare the RNA extraction kits, we simultaneously captured two adjacent brain regions, hippocampal subregions CA1 and CA2 (Figures ), using the PixCell IIe instrument and isolated RNA using either the PicoPure (CA1 samples) or QIAGEN (CA2 samples) kits. .. This was true when using either extraction kit, however, we note that our PixCell PicoPure samples consisted solely of samples captured from brain tissue and our CellCut QIAGEN samples were entirely from liver tissue (See Table ).

    Article Title: Functional Polymorphism of the CK2? Intronless Gene Plays Oncogenic Roles in Lung Cancer
    Article Snippet: .. Semi-quantitative reverse transcription-PCR (RT-PCR) analysis Total RNA from cell lines and tissues was isolated using an extraction kit, and DNA was eliminated by on-column treatment with DNase (RNeasy Mini kit; Qiagen, Valencia, CA, USA). .. Semiquantitative RT-PCR was performed by using SuperScript One-step RT-PCR with Platinum Taq kit (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol.

    Article Title: Characterization of microflora in Latin-style cheeses by next-generation sequencing technology
    Article Snippet: Additionally, Renye et al. found more variety in the types of bacteria isolated from cheeses made with raw milk versus those made with pasteurized milk [ ]; another public health risk best evaluated with tools that can distinguish between live and dead cells. .. First, the extraction kit used to perform the DNA extractions was chosen based on data collected in which the Qiagen DNeasy Blood and Tissue kit was compared to five other commercially-available kits for the extraction of Brucella neotomae DNA from the same Latin-style cheeses used in this study (T. Lusk, E. Strain, and J.A.

    Article Title: PSIII-25 Adipose gene expression of mature neutered male cats during the transition from a lean to an obese phenotype
    Article Snippet: .. RNA was isolated using a commercially available extraction kit specific for lipids (RNeasy lipid tissue mini kit, Qiagen). mRNA expression of over 40 genes encoding adipokines, transcription factors, glucose transporters, inflammatory processes, and metabolic enzymes was measured by Fluidigm multiplex qPCR. .. This generated a gene expression profile portraying the upregulation of genes associated with energy storage but not energy mobilization.

    Article Title: Glutamate signaling through the NMDA receptor reduces the expression of scleraxis in plantaris tendon derived cells
    Article Snippet: .. RNA isolation, reverse transcription, qPCR For the extraction of RNA, an extraction kit was used (code: 74106; Qiagen) and the protocol from the manufactures was followed. .. The isolated RNA was then reverse transcribed into cDNA via a High Capacity cDNA Reverse Transcription kit (code: 4368813; Applied Biosystems).

    Article Title: Akonni TruTip® and Qiagen® Methods for Extraction of Fetal Circulating DNA - Evaluation by Real-Time and Digital PCR
    Article Snippet: .. At the time of this study, the only commercially available extraction kit for the isolation of cell-free DNA able to accommodate volumes of at least 5 mL was the manual Qiagen Circulating Nucleic Acid Kit, which uses silica filter spin columns [ ]. .. This method was compared to the newly developed technology from Akonni Biosystems, the TruTip® extraction method [ ] that also uses the BOOM® silica binding chemistry [ ] for nucleic acid binding and elution.

    RNA Extraction:

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
    Article Snippet: Paragraph title: Type of Laser and RNA Extraction Method Impact RNA Quality ... This was true when using either extraction kit, however, we note that our PixCell PicoPure samples consisted solely of samples captured from brain tissue and our CellCut QIAGEN samples were entirely from liver tissue (See Table ).

    Article Title: RNA sequencing dataset describing transcriptional changes in cervical dorsal root ganglia after bilateral pyramidotomy and forelimb intramuscular gene therapy with an adeno-associated viral vector encoding human neurotrophin-3
    Article Snippet: Paragraph title: 2.1. Total RNA extraction for sequencing ... 1.5 volumes of 100% ethanol were added to the aqueous phase and transferred to filtered spin columns from the extraction kit for poly A RNA and small RNAs (miRNeasy kit, Qiagen, 217004) and we proceeded according to manufacturer׳s instructions.

    Article Title: Exacerbation of Autoimmune Neuro-Inflammation in Mice Cured from Blood-Stage Plasmodium berghei Infection
    Article Snippet: .. RNA extraction from DP-T cells was carried our immediately after sorting, using an extraction kit according to the manufactureŕs instruction (RNeasy Micro kit, Qiagen USA). .. Expression of AIRE (Mm00477461_m1), IL-7 (Mm01295803_m1), IL-6 (Mm00446190_m1), IL-17 (Mm00439618_m1), IFNg (Mm01168134_m1), FOXP3 (Mm00475162_m1) and RAR-related orphan receptor C (RORc) (Mm01261022_m1) were analyzed in comparison to GAPDH (Mm99999915_g1, housekeeping gene) levels.

    Mouse Assay:

    Article Title: Exacerbation of Autoimmune Neuro-Inflammation in Mice Cured from Blood-Stage Plasmodium berghei Infection
    Article Snippet: RT-PCR assays Mice were killed at the indicated time-points and frozen tissues were used for RNA extraction (Trizol reagent, Life Technologies, USA) and cDNA synthesis according to the manufactureŕs recommendations (High Capacity RNA-to-cDNA converter kit, Life Techcnologies, USA). .. RNA extraction from DP-T cells was carried our immediately after sorting, using an extraction kit according to the manufactureŕs instruction (RNeasy Micro kit, Qiagen USA).

    Sequencing:

    Article Title: RNA sequencing dataset describing transcriptional changes in cervical dorsal root ganglia after bilateral pyramidotomy and forelimb intramuscular gene therapy with an adeno-associated viral vector encoding human neurotrophin-3
    Article Snippet: Paragraph title: 2.1. Total RNA extraction for sequencing ... 1.5 volumes of 100% ethanol were added to the aqueous phase and transferred to filtered spin columns from the extraction kit for poly A RNA and small RNAs (miRNeasy kit, Qiagen, 217004) and we proceeded according to manufacturer׳s instructions.

    Article Title: 1,4‐Dioxane‐degrading consortia can be enriched from uncontaminated soils: prevalence of Mycobacterium and soluble di‐iron monooxygenase genes
    Article Snippet: Microbial community analyses 16S rRNA gene sequencing was used to determine the microbial community composition of the original soil samples and the enriched two consortia. .. The extraction kit was the UltraClean® Microbial DNA Isolation Kit (MO BIO, Carlsbad, CA, USA).

    Purification:

    Article Title: Akonni TruTip® and Qiagen® Methods for Extraction of Fetal Circulating DNA - Evaluation by Real-Time and Digital PCR
    Article Snippet: All of these technologies and applications require up-front purification and concentration of the sample to be analyzed, which stresses the importance of finding a method capable of efficient and reliable recovery of freely circulating DNA. .. At the time of this study, the only commercially available extraction kit for the isolation of cell-free DNA able to accommodate volumes of at least 5 mL was the manual Qiagen Circulating Nucleic Acid Kit, which uses silica filter spin columns [ ].

    Multiplex Assay:

    Article Title: PSIII-25 Adipose gene expression of mature neutered male cats during the transition from a lean to an obese phenotype
    Article Snippet: .. RNA was isolated using a commercially available extraction kit specific for lipids (RNeasy lipid tissue mini kit, Qiagen). mRNA expression of over 40 genes encoding adipokines, transcription factors, glucose transporters, inflammatory processes, and metabolic enzymes was measured by Fluidigm multiplex qPCR. .. This generated a gene expression profile portraying the upregulation of genes associated with energy storage but not energy mobilization.

    Sample Prep:

    Article Title: Characterization of microflora in Latin-style cheeses by next-generation sequencing technology
    Article Snippet: Efforts exist to manipulate sample preparation to detect only cells with intact membranes by sample treatment with propidium monoazide in combination with PCR amplification [ ] or the generation of transcriptomes. .. First, the extraction kit used to perform the DNA extractions was chosen based on data collected in which the Qiagen DNeasy Blood and Tissue kit was compared to five other commercially-available kits for the extraction of Brucella neotomae DNA from the same Latin-style cheeses used in this study (T. Lusk, E. Strain, and J.A.

    Next-Generation Sequencing:

    Article Title: Characterization of microflora in Latin-style cheeses by next-generation sequencing technology
    Article Snippet: This will improve NGS as a tool for assessing microflora of cheese at different stages of the aging process. .. First, the extraction kit used to perform the DNA extractions was chosen based on data collected in which the Qiagen DNeasy Blood and Tissue kit was compared to five other commercially-available kits for the extraction of Brucella neotomae DNA from the same Latin-style cheeses used in this study (T. Lusk, E. Strain, and J.A.

    Laser Capture Microdissection:

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
    Article Snippet: When comparing the CellCut vs. the PixCell LCM instruments, we found that the PixCell instrument produced samples with significantly higher quality RNA (Figure , p < 0.0001, CellCut vs. PixCell F (1,119) = 114.6, two-way ANOVA). .. This was true when using either extraction kit, however, we note that our PixCell PicoPure samples consisted solely of samples captured from brain tissue and our CellCut QIAGEN samples were entirely from liver tissue (See Table ).

    Spectrophotometry:

    Article Title: RNA sequencing dataset describing transcriptional changes in cervical dorsal root ganglia after bilateral pyramidotomy and forelimb intramuscular gene therapy with an adeno-associated viral vector encoding human neurotrophin-3
    Article Snippet: 1.5 volumes of 100% ethanol were added to the aqueous phase and transferred to filtered spin columns from the extraction kit for poly A RNA and small RNAs (miRNeasy kit, Qiagen, 217004) and we proceeded according to manufacturer׳s instructions. .. We estimated the total RNA quality and quantity by spectrophotometry (NanoDrop, ND-1000) and measured the RNA integrity numbers (RINs) (Agilent RNA 6000 Nano Reagents Part I and Agilent 2100 Bioanalyzer).

    Sampling:

    Article Title: Responding to ever-changing epidemiological dynamics of Ebola virus disease
    Article Snippet: The RT-LAMP test kit has also been used with timely sampling with oral swabs as a postmortem diagnostic to purposively support safe burial in communities in Guinea. .. Recently, RT-LAMP in combination with a new extraction kit, SpeedXtract (Qiagen, Germany), and an improved diagnostic reverse transcription recombinase polymerase amplification assay (EBOV-RT-RPA), has been validated on sera and swabs in Guinea for case management as well as for safe burial management.

    Article Title: A New Subspecies Identification and Population Study of the Asian Small-Clawed Otter (Aonyx cinereus) in Malay Peninsula and Southern Thailand Based on Fecal DNA Method
    Article Snippet: Paragraph title: 2.1. Sampling Locations, Samples, and DNA Isolation ... Total genomic DNA was extracted by using the standard extraction kit and the protocol provided by the Qiagen DNeasy Stool Kit [ ].

    Produced:

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
    Article Snippet: When comparing the CellCut vs. the PixCell LCM instruments, we found that the PixCell instrument produced samples with significantly higher quality RNA (Figure , p < 0.0001, CellCut vs. PixCell F (1,119) = 114.6, two-way ANOVA). .. This was true when using either extraction kit, however, we note that our PixCell PicoPure samples consisted solely of samples captured from brain tissue and our CellCut QIAGEN samples were entirely from liver tissue (See Table ).

    Concentration Assay:

    Article Title: Akonni TruTip® and Qiagen® Methods for Extraction of Fetal Circulating DNA - Evaluation by Real-Time and Digital PCR
    Article Snippet: All of these technologies and applications require up-front purification and concentration of the sample to be analyzed, which stresses the importance of finding a method capable of efficient and reliable recovery of freely circulating DNA. .. At the time of this study, the only commercially available extraction kit for the isolation of cell-free DNA able to accommodate volumes of at least 5 mL was the manual Qiagen Circulating Nucleic Acid Kit, which uses silica filter spin columns [ ].

    Lysis:

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
    Article Snippet: These kits differ in the recommended time and temperature of tissue lysis with the PicoPure lysis incubation being 30 min at 42°C and the QIAGEN lysis incubation conditions being 5 min at room temperature. .. This was true when using either extraction kit, however, we note that our PixCell PicoPure samples consisted solely of samples captured from brain tissue and our CellCut QIAGEN samples were entirely from liver tissue (See Table ).

    Fluorescence In Situ Hybridization:

    Article Title: A New Subspecies Identification and Population Study of the Asian Small-Clawed Otter (Aonyx cinereus) in Malay Peninsula and Southern Thailand Based on Fecal DNA Method
    Article Snippet: In this preliminary study, sampling locations were selected based on several reports of the existence of otters either by local people or parties, mainly at industrial fish ponds, lakes, and riversides within the same period of time. .. Total genomic DNA was extracted by using the standard extraction kit and the protocol provided by the Qiagen DNeasy Stool Kit [ ].

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    Qiagen qiaquick gel extractin kit
    Qiaquick Gel Extractin Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaquick gel extractin kit/product/Qiagen
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    Qiagen gel extracion kit
    Gel Extracion Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gel extracion kit/product/Qiagen
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gel extracion kit - by Bioz Stars, 2020-03
    84/100 stars
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    79
    Qiagen no 114391 5g qiaquick gel extractio kit
    No 114391 5g Qiaquick Gel Extractio Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/no 114391 5g qiaquick gel extractio kit/product/Qiagen
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    no 114391 5g qiaquick gel extractio kit - by Bioz Stars, 2020-03
    79/100 stars
      Buy from Supplier

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