extensor long range pcr enzyme master mix  (Thermo Fisher)

 
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    Name:
    Extensor Long PCR Enzyme Mix Products
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    Catalog Number:
    ab-0720/b
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    None
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    Structured Review

    Thermo Fisher extensor long range pcr enzyme master mix

    https://www.bioz.com/result/extensor long range pcr enzyme master mix/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    extensor long range pcr enzyme master mix - by Bioz Stars, 2021-02
    93/100 stars

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    pcrs
    q5 high-fidelity dna polymerase

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Genetic and neural dissociation of individual responses to emotional expressions in human infants
    Article Snippet: .. PCR-amplification for genotyping the rs4795541 5-HTTLPR indel polymorphism was carried out using the conditions described above, except after the initial denaturation and activation step, there were 34 repeated cycles of denaturation at 95 °C for 30 s, an annealing step for 30 s min at 66 °C and extension at 72 °C for 45 s. The reactions included 20 ng of template DNA, 1× PCR buffer mix 1 (ABgene, Hamburg, Germany), 500 μM dNTPs (Amersham Biosciences, Uppsala, Sweden), 400 nM of each primer (Biotez, Berlin, Germany) and 1.25 unit of Extensor Long PCR Enzyme (ABgene, Hamburg, Germany). ..

    Article Title: RUNX2 tandem repeats and the evolution of facial length in placental mammals
    Article Snippet: .. PCRs were performed using the Extensor Hi-Fidelity PCR enzyme (Thermo Scientific). .. An external touchdown PCR using primers Sears Ext F (5’-TTGTGATGCGTATTCCCGTA-3’) or Sears Int F (5’ATCCGAGCACCAGCCGGCGGCGCTTCAG-5’) with Sears Ext R (5’-ACSGAGCACAGGAAGTTGGG-3’) was performed on approximately 100ng of template DNA with the following cycling conditions: 95°C 2mins, 15x (95°C 30s, 61.3 (down 0.5°C per cycle) 30s, 72°C 50s), 20x (95°C 30s, 54.3°C 30s, 72°C 50s), 72°C 5 mins final extension.

    Article Title: miR-224 overexpression is a strong and independent prognosticator of short-term relapse and poor overall survival in colorectal adenocarcinoma.
    Article Snippet: .. The cycling conditions were as follows: a denaturation step at 95˚C for 3 min, followed by 40 cycles of 95˚C for 3 sec, for denaturation of the PCR prod- ucts, and 60˚C for 30 sec, for primer annealing and extension. .. Each real-time PCR reaction was performed in duplicate to evaluate the reproducibility of data (Fig. 1A).

    Article Title: A highly abundant bacteriophage discovered in the unknown sequences of human faecal metagenomes
    Article Snippet: .. The PCR was performed using the Thermo Scientific Hi-Fidelity Extensor Long-Range PCR Enzyme kit with 9 μl of the 2 × mix and 14 μl of water per reaction (Cat. No. AB-0792/A and AB-0720/B). .. A touchdown method was used for the PCR cycling conditions to account for differing melting temperatures of the primers.

    Article Title: Unusual genome complexity in Lactobacillus salivarius JCM1046
    Article Snippet: .. Scaffolds were ordered and oriented by PCR using primers were designed at the ends of the scaffolds and the inter-scaffold region was amplified using Extensor long PCR enzyme mix (Abgene, Epsom, UK). .. PCR products for both the sequencing gaps and the inter-scaffold gaps were sequenced by Eurofins MWG Operon (Ebersberg, Germany) and the sequences were integrated into the assembly using PHRAP [ ].

    Article Title: Epigenetic Control of Chromosome Breakage at the 5? End of Paramecium tetraurelia Gene A
    Article Snippet: .. Polymerase reactions and PCRs were performed either with enzyme Tfl (Promega) or the PCR Mix Extensor (ABgene). .. PCR products were purified using the Geneclean (Bio 101, Inc.) or Qiaquick (Qiagen) procedure.

    Article Title: Nucleotide Parasitism by Simkania negevensis (Chlamydiae) ▿) ▿ †
    Article Snippet: .. The Sn NTT1 to Sn NTT4 genes were amplified with the Extensor Hi-Fidelity PCR Enzyme Mix (ABgene), and the primers used for the amplification of the genes were designed based on draft genome sequence data for S. negevensis strain Z (see Table S1 in the supplemental material). ..

    Article Title: Genetic Diversity: Frameshift Mechanisms Alter Coding of a Gene (Epstein-Barr Virus LF3 Gene) That Contains Multiple 102-Base-Pair Direct Sequence Repeats
    Article Snippet: .. For amplification, the Extensor Hi-Fidelity PCR enzyme (ABgene) was used because of its low error rate, in the range of 5 × 10−5 to 3 × 10−6 errors/base, depending on the nature of the template (information from the manufacturers). .. Cloned DNA products were sequenced.

    Activation Assay:

    Article Title: Genetic and neural dissociation of individual responses to emotional expressions in human infants
    Article Snippet: .. PCR-amplification for genotyping the rs4795541 5-HTTLPR indel polymorphism was carried out using the conditions described above, except after the initial denaturation and activation step, there were 34 repeated cycles of denaturation at 95 °C for 30 s, an annealing step for 30 s min at 66 °C and extension at 72 °C for 45 s. The reactions included 20 ng of template DNA, 1× PCR buffer mix 1 (ABgene, Hamburg, Germany), 500 μM dNTPs (Amersham Biosciences, Uppsala, Sweden), 400 nM of each primer (Biotez, Berlin, Germany) and 1.25 unit of Extensor Long PCR Enzyme (ABgene, Hamburg, Germany). ..

    Amplification:

    Article Title: Unusual genome complexity in Lactobacillus salivarius JCM1046
    Article Snippet: .. Scaffolds were ordered and oriented by PCR using primers were designed at the ends of the scaffolds and the inter-scaffold region was amplified using Extensor long PCR enzyme mix (Abgene, Epsom, UK). .. PCR products for both the sequencing gaps and the inter-scaffold gaps were sequenced by Eurofins MWG Operon (Ebersberg, Germany) and the sequences were integrated into the assembly using PHRAP [ ].

    Article Title: Nucleotide Parasitism by Simkania negevensis (Chlamydiae) ▿) ▿ †
    Article Snippet: .. The Sn NTT1 to Sn NTT4 genes were amplified with the Extensor Hi-Fidelity PCR Enzyme Mix (ABgene), and the primers used for the amplification of the genes were designed based on draft genome sequence data for S. negevensis strain Z (see Table S1 in the supplemental material). ..

    Article Title: Genetic Diversity: Frameshift Mechanisms Alter Coding of a Gene (Epstein-Barr Virus LF3 Gene) That Contains Multiple 102-Base-Pair Direct Sequence Repeats
    Article Snippet: .. For amplification, the Extensor Hi-Fidelity PCR enzyme (ABgene) was used because of its low error rate, in the range of 5 × 10−5 to 3 × 10−6 errors/base, depending on the nature of the template (information from the manufacturers). .. Cloned DNA products were sequenced.

    Sequencing:

    Article Title: Nucleotide Parasitism by Simkania negevensis (Chlamydiae) ▿) ▿ †
    Article Snippet: .. The Sn NTT1 to Sn NTT4 genes were amplified with the Extensor Hi-Fidelity PCR Enzyme Mix (ABgene), and the primers used for the amplification of the genes were designed based on draft genome sequence data for S. negevensis strain Z (see Table S1 in the supplemental material). ..

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  • 93
    Thermo Fisher extensor long range pcr enzyme master mix
    Extensor Long Range Pcr Enzyme Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/extensor long range pcr enzyme master mix/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    extensor long range pcr enzyme master mix - by Bioz Stars, 2021-02
    93/100 stars
      Buy from Supplier

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