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TaKaRa extaq hot start version
Extaq Hot Start Version, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/extaq hot start version/product/TaKaRa
Average 95 stars, based on 16 article reviews
Price from $9.99 to $1999.99
extaq hot start version - by Bioz Stars, 2020-04
95/100 stars

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Clone Assay:

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: Cloning of miRNAs was performed using a small RNA Cloning Kit (Takara Bio, Shiga, Japan) according to the manufacturer's instructions ( ). .. Finally, miRNAs were transcribed to cDNA and amplified using ExTaq Hot Start Version (Takara Bio).

Article Title: Direct detection of falciparum and non-falciparum malaria DNA from a drop of blood with high sensitivity by the dried-LAMP system
Article Snippet: All PCR reactions were carried out with ExTaq Hot Start Version (Takara-Bio, Shiga, Japan) in a volume of 20 μl. .. In case of detection of mixed wave patterns in the DNA sequencing, the amplicon was cloned into pGEM-T Easy vector (Promega, USA), and then several clones were sequenced.

Amplification:

Article Title: Fc gamma receptor IIb participates in maternal IgG trafficking of human placental endothelial cells
Article Snippet: .. For conventional RT-PCR, ExTaq Hot Start Version (Takara Bio) was used for amplification using gene-specific primers, some of which were designed using DNASIS software (Hitachi, Tokyo, Japan). ..

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: .. Purified 2nd PCR products were amplified using ExTaq Hot Start Version (Takara Bio) with the same conditions as for the 1st PCR methods. ..

Article Title: An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis
Article Snippet: .. A 49.4 µl volume of PCR mixture III [1× ExTaq buffer, 0.25 mM each of dATP, dCTP, dGTP and dTTP, 0.02 µg/µl primer T7-V1 (5′-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGATATGGATCCGGCGCGCCGTCGAC-3′), 0.02 µg/µl primer V3 (dT)24 and 0.05 U/µl ExTaq Hot Start Version] was added to each of eight 0.2-ml thin-walled PCR tubes containing 0.63 µl of the 20 cycle amplified cDNA. .. A nine-cycle amplification was then performed according to the following schedule: 95°C for 5 min 30 s, 64°C for 1 min and 72°C for 5 min 18 s for the first cycle; and 95°C for 30 s, 67°C for 1 min and 72°C for 5 min 18 s with an extension of 6 s per cycle for another eight cycles.

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: .. Finally, miRNAs were transcribed to cDNA and amplified using ExTaq Hot Start Version (Takara Bio). ..

Article Title: Historical Changes in the Ecosystem Condition of a Small Mountain Lake over the Past 60 Years as Revealed by Plankton Remains and Daphnia Ephippial Carapaces Stored in Lake Sediments
Article Snippet: .. PCR amplification of 12S rDNA fragments was carried out according to protocol III (using Type-it Microsatellite PCR Kit (Qiagen) and ExTaq Hot Start Version (Takara)) described in Ishida et al. [ ] with the primer set composed of d5f and d7r. ..

Article Title: Direct detection of falciparum and non-falciparum malaria DNA from a drop of blood with high sensitivity by the dried-LAMP system
Article Snippet: All PCR reactions were carried out with ExTaq Hot Start Version (Takara-Bio, Shiga, Japan) in a volume of 20 μl. .. In case of detection of mixed wave patterns in the DNA sequencing, the amplicon was cloned into pGEM-T Easy vector (Promega, USA), and then several clones were sequenced.

Synthesized:

Article Title: Fc gamma receptor IIb participates in maternal IgG trafficking of human placental endothelial cells
Article Snippet: For conventional RT-PCR, ExTaq Hot Start Version (Takara Bio) was used for amplification using gene-specific primers, some of which were designed using DNASIS software (Hitachi, Tokyo, Japan). .. All primers were synthesized by Nippon EGT.

Cytometry:

Article Title: Lack of a significant impact of Gag-Protease-mediated HIV-1 replication capacity on clinical parameters in treatment-naive Japanese individuals
Article Snippet: Plasma-derived gag -protease DNA fragments were generated from the cDNAs by nested PCR, using TaKaRa ExTaq Hot Start Version (Takara Bio Inc., Shiga, Japan). .. Stock titers were determined by infecting CEM-GXR and measuring GFP+ cells, using flow cytometry.

Quantitative RT-PCR:

Article Title: Fc gamma receptor IIb participates in maternal IgG trafficking of human placental endothelial cells
Article Snippet: Paragraph title: RT-qPCR ... For conventional RT-PCR, ExTaq Hot Start Version (Takara Bio) was used for amplification using gene-specific primers, some of which were designed using DNASIS software (Hitachi, Tokyo, Japan).

Electrophoresis:

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: Second PCR products (approximately 65 bp) were separated by electrophoresis on a 10% TBE gel stained with SYBR Gold nucleic acid gel stain (Invitrogen, Carlsbad, CA, USA). .. Purified 2nd PCR products were amplified using ExTaq Hot Start Version (Takara Bio) with the same conditions as for the 1st PCR methods.

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: Finally, miRNAs were transcribed to cDNA and amplified using ExTaq Hot Start Version (Takara Bio). .. Second PCR products (approximately 65 bp) were separated by electrophoresis on a 10% TBE gel stained with SYBR Gold nucleic acid gel stain (Invitrogen, Carlsbad, CA, USA).

Incubation:

Article Title: Historical Changes in the Ecosystem Condition of a Small Mountain Lake over the Past 60 Years as Revealed by Plankton Remains and Daphnia Ephippial Carapaces Stored in Lake Sediments
Article Snippet: These were incubated at 95°C for 30 min. and stored on ice for > 3 min. A further 50 μl of a neutralizing buffer (40 mM Tris-HCl, pH 5) was added to each tube. .. PCR amplification of 12S rDNA fragments was carried out according to protocol III (using Type-it Microsatellite PCR Kit (Qiagen) and ExTaq Hot Start Version (Takara)) described in Ishida et al. [ ] with the primer set composed of d5f and d7r.

Modification:

Article Title: Recurrent alterations of the WW domain containing oxidoreductase gene spanning the common fragile site FRA16D in multiple myeloma and monoclonal gammopathy of undetermined significance
Article Snippet: Each 0.5 µg sample of genomic DNA was treated with sodium bisulfite using the MethylEasy Xceed Rapid DNA Bisulfite Modification kit (Takara Bio, Inc.) following manufacturer's protocol, and the converted DNA was subjected to PCR. .. Each reaction (20 µl each) contained 0.2 µmol of each primer, 2.0 mM MgCl2 , 0.2 mM dNTP mix, 1X PCR buffer and 1.25 units of Takara ExTaq Hot Start Version (Takara Bio, Inc.).

Flow Cytometry:

Article Title: Lack of a significant impact of Gag-Protease-mediated HIV-1 replication capacity on clinical parameters in treatment-naive Japanese individuals
Article Snippet: Plasma-derived gag -protease DNA fragments were generated from the cDNAs by nested PCR, using TaKaRa ExTaq Hot Start Version (Takara Bio Inc., Shiga, Japan). .. Stock titers were determined by infecting CEM-GXR and measuring GFP+ cells, using flow cytometry.

Ligation:

Article Title: The tRNA-Derived Small RNAs Regulate Gene Expression through Triggering Sequence-Specific Degradation of Target Transcripts in the Oomycete Pathogen Phytophthora sojae
Article Snippet: The ligation products were extracted by phenol/chloroform and precipitated with glycogen before synthesis of the first-strand of cDNA by using SuperScript II Reverse Transcriptase (Invitrogen, USA). .. A nested PCR with ExTaq Hot Start Version (TaKaRa, China) was used to detect the degraded products with primers RA44OP/IP and GSP1/GSP2.

Infection:

Article Title: Direct detection of falciparum and non-falciparum malaria DNA from a drop of blood with high sensitivity by the dried-LAMP system
Article Snippet: Nested PCR detection of non-P. falciparum infection was double-checked by mtDNA nested PCR primers newly designed in this study by using Primer 3 online program (Table ). .. All PCR reactions were carried out with ExTaq Hot Start Version (Takara-Bio, Shiga, Japan) in a volume of 20 μl.

Generated:

Article Title: Lack of a significant impact of Gag-Protease-mediated HIV-1 replication capacity on clinical parameters in treatment-naive Japanese individuals
Article Snippet: .. Plasma-derived gag -protease DNA fragments were generated from the cDNAs by nested PCR, using TaKaRa ExTaq Hot Start Version (Takara Bio Inc., Shiga, Japan). .. Chimeric HIV-1NL4-3 encoding plasma-derived gag -protease was generated by electroporating gag -protease PCR fragments with linearized gag -protease -deficient pNL4-3 (delta gag -pro NL4-3) into a CEM-derived GFP-reporter T-cell line engineered to carry LTR- G FP, C X CR4, and CC R 5 (CEM-GXR, a generous gift from T. Miura and M.A.

Polymerase Chain Reaction:

Article Title: Lack of a significant impact of Gag-Protease-mediated HIV-1 replication capacity on clinical parameters in treatment-naive Japanese individuals
Article Snippet: Plasma-derived gag -protease DNA fragments were generated from the cDNAs by nested PCR, using TaKaRa ExTaq Hot Start Version (Takara Bio Inc., Shiga, Japan). .. Chimeric HIV-1NL4-3 encoding plasma-derived gag -protease was generated by electroporating gag -protease PCR fragments with linearized gag -protease -deficient pNL4-3 (delta gag -pro NL4-3) into a CEM-derived GFP-reporter T-cell line engineered to carry LTR- G FP, C X CR4, and CC R 5 (CEM-GXR, a generous gift from T. Miura and M.A.

Article Title: Evaluation of repetitive-PCR and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for rapid strain typing of Bacillus coagulans
Article Snippet: .. PCR was done with TaKaRa ExTaq Hot Start Version (Takara Bio, Shiga, Japan), template DNA, and the primers in a reaction volume of 50 μL. .. The PCR conditions were initial denaturation at 94°C for 2 min, then 30 cycles of denaturation at 94°C for 1 min, annealing at 55°C for 1 min, and extension at 72°C for 1 min, and final extension at 72°C for 2 min.

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: .. Purified 2nd PCR products were amplified using ExTaq Hot Start Version (Takara Bio) with the same conditions as for the 1st PCR methods. ..

Article Title: An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis
Article Snippet: .. A 49.4 µl volume of PCR mixture III [1× ExTaq buffer, 0.25 mM each of dATP, dCTP, dGTP and dTTP, 0.02 µg/µl primer T7-V1 (5′-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGATATGGATCCGGCGCGCCGTCGAC-3′), 0.02 µg/µl primer V3 (dT)24 and 0.05 U/µl ExTaq Hot Start Version] was added to each of eight 0.2-ml thin-walled PCR tubes containing 0.63 µl of the 20 cycle amplified cDNA. .. A nine-cycle amplification was then performed according to the following schedule: 95°C for 5 min 30 s, 64°C for 1 min and 72°C for 5 min 18 s for the first cycle; and 95°C for 30 s, 67°C for 1 min and 72°C for 5 min 18 s with an extension of 6 s per cycle for another eight cycles.

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: Finally, miRNAs were transcribed to cDNA and amplified using ExTaq Hot Start Version (Takara Bio). .. Amplification was performed for 15 cycles with annealing at 60°C for 30 s, extension at 72°C for 30 s, and a final extension for 3 min. First PCR products were amplified with the same conditions as for the 1st PCR methods.

Article Title: Historical Changes in the Ecosystem Condition of a Small Mountain Lake over the Past 60 Years as Revealed by Plankton Remains and Daphnia Ephippial Carapaces Stored in Lake Sediments
Article Snippet: .. PCR amplification of 12S rDNA fragments was carried out according to protocol III (using Type-it Microsatellite PCR Kit (Qiagen) and ExTaq Hot Start Version (Takara)) described in Ishida et al. [ ] with the primer set composed of d5f and d7r. ..

Article Title: Recurrent alterations of the WW domain containing oxidoreductase gene spanning the common fragile site FRA16D in multiple myeloma and monoclonal gammopathy of undetermined significance
Article Snippet: .. Each reaction (20 µl each) contained 0.2 µmol of each primer, 2.0 mM MgCl2 , 0.2 mM dNTP mix, 1X PCR buffer and 1.25 units of Takara ExTaq Hot Start Version (Takara Bio, Inc.). .. The thermocycling conditions maintained were as follows: 95°C for 8 min; 35 cycles at 94°C for 30 sec, 58°C for 30 sec, and 72°C for 1 min; and an extension step of 72°C for 5 min.

DNA Sequencing:

Article Title: Historical Changes in the Ecosystem Condition of a Small Mountain Lake over the Past 60 Years as Revealed by Plankton Remains and Daphnia Ephippial Carapaces Stored in Lake Sediments
Article Snippet: PCR amplification of 12S rDNA fragments was carried out according to protocol III (using Type-it Microsatellite PCR Kit (Qiagen) and ExTaq Hot Start Version (Takara)) described in Ishida et al. [ ] with the primer set composed of d5f and d7r. .. DNA sequencing reaction was performed using a BigDye Terminator v3.1 Cycle Sequencing Ready Reaction Kit (Applied Biosystems) and each PCR product purified by CleanSEQ (Agencourt Bioscience).

Article Title: Direct detection of falciparum and non-falciparum malaria DNA from a drop of blood with high sensitivity by the dried-LAMP system
Article Snippet: All PCR reactions were carried out with ExTaq Hot Start Version (Takara-Bio, Shiga, Japan) in a volume of 20 μl. .. In case of detection of mixed wave patterns in the DNA sequencing, the amplicon was cloned into pGEM-T Easy vector (Promega, USA), and then several clones were sequenced.

Sequencing:

Article Title: Evaluation of repetitive-PCR and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for rapid strain typing of Bacillus coagulans
Article Snippet: Polymorphism analysis of the internal transcribed spacer (ITS) region of the 16S-23S ribosomal DNA sequence was performed according to the method of Daffonchio et al. [ ]. .. PCR was done with TaKaRa ExTaq Hot Start Version (Takara Bio, Shiga, Japan), template DNA, and the primers in a reaction volume of 50 μL.

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: Paragraph title: MiRNA library construction and high‐throughput whole‐miRNAs sequencing ... Purified 2nd PCR products were amplified using ExTaq Hot Start Version (Takara Bio) with the same conditions as for the 1st PCR methods.

Article Title: The tRNA-Derived Small RNAs Regulate Gene Expression through Triggering Sequence-Specific Degradation of Target Transcripts in the Oomycete Pathogen Phytophthora sojae
Article Snippet: A nested PCR with ExTaq Hot Start Version (TaKaRa, China) was used to detect the degraded products with primers RA44OP/IP and GSP1/GSP2. .. The amplicons were further confirmed by sequencing.

Article Title: Historical Changes in the Ecosystem Condition of a Small Mountain Lake over the Past 60 Years as Revealed by Plankton Remains and Daphnia Ephippial Carapaces Stored in Lake Sediments
Article Snippet: PCR amplification of 12S rDNA fragments was carried out according to protocol III (using Type-it Microsatellite PCR Kit (Qiagen) and ExTaq Hot Start Version (Takara)) described in Ishida et al. [ ] with the primer set composed of d5f and d7r. .. DNA sequencing reaction was performed using a BigDye Terminator v3.1 Cycle Sequencing Ready Reaction Kit (Applied Biosystems) and each PCR product purified by CleanSEQ (Agencourt Bioscience).

Sonication:

Article Title: Historical Changes in the Ecosystem Condition of a Small Mountain Lake over the Past 60 Years as Revealed by Plankton Remains and Daphnia Ephippial Carapaces Stored in Lake Sediments
Article Snippet: The samples were then vortexed followed by sonication for 1 min. using a USP-50 ultrasonic homogenizer (Shimadzu). .. PCR amplification of 12S rDNA fragments was carried out according to protocol III (using Type-it Microsatellite PCR Kit (Qiagen) and ExTaq Hot Start Version (Takara)) described in Ishida et al. [ ] with the primer set composed of d5f and d7r.

DNA Extraction:

Article Title: Historical Changes in the Ecosystem Condition of a Small Mountain Lake over the Past 60 Years as Revealed by Plankton Remains and Daphnia Ephippial Carapaces Stored in Lake Sediments
Article Snippet: Genetic analysis DNA extraction from ECs was performed by the method described in Ishida et al. [ ]. .. PCR amplification of 12S rDNA fragments was carried out according to protocol III (using Type-it Microsatellite PCR Kit (Qiagen) and ExTaq Hot Start Version (Takara)) described in Ishida et al. [ ] with the primer set composed of d5f and d7r.

Methylation:

Article Title: Recurrent alterations of the WW domain containing oxidoreductase gene spanning the common fragile site FRA16D in multiple myeloma and monoclonal gammopathy of undetermined significance
Article Snippet: Paragraph title: Methylation-specific PCR (MSP) ... Each reaction (20 µl each) contained 0.2 µmol of each primer, 2.0 mM MgCl2 , 0.2 mM dNTP mix, 1X PCR buffer and 1.25 units of Takara ExTaq Hot Start Version (Takara Bio, Inc.).

Isolation:

Article Title: The tRNA-Derived Small RNAs Regulate Gene Expression through Triggering Sequence-Specific Degradation of Target Transcripts in the Oomycete Pathogen Phytophthora sojae
Article Snippet: 5′ RLM-RACE Analysis To perform 5′ RNA ligase -mediated rapid amplification of cDNA ends (5′ RLM-RACE), the poly (A)+ RNA was isolated by using Magnetic mRNA Isolation Kit (NEB, UK) from 200 μg total RNA. .. A nested PCR with ExTaq Hot Start Version (TaKaRa, China) was used to detect the degraded products with primers RA44OP/IP and GSP1/GSP2.

Size-exclusion Chromatography:

Article Title: Historical Changes in the Ecosystem Condition of a Small Mountain Lake over the Past 60 Years as Revealed by Plankton Remains and Daphnia Ephippial Carapaces Stored in Lake Sediments
Article Snippet: In short, regardless of intact of the resting eggs, ECs were individually transferred into a 0.2 ml tube containing 50 μl of alkaline lysis buffer (25 mM NaOH and 0.2 mM of disodium EDTA, pH 12) and subjected to five cycles of thermal shock consisting of 5 min. at −80°C and 20 sec. at 70°C. .. PCR amplification of 12S rDNA fragments was carried out according to protocol III (using Type-it Microsatellite PCR Kit (Qiagen) and ExTaq Hot Start Version (Takara)) described in Ishida et al. [ ] with the primer set composed of d5f and d7r.

Article Title: Recurrent alterations of the WW domain containing oxidoreductase gene spanning the common fragile site FRA16D in multiple myeloma and monoclonal gammopathy of undetermined significance
Article Snippet: Each reaction (20 µl each) contained 0.2 µmol of each primer, 2.0 mM MgCl2 , 0.2 mM dNTP mix, 1X PCR buffer and 1.25 units of Takara ExTaq Hot Start Version (Takara Bio, Inc.). .. The thermocycling conditions maintained were as follows: 95°C for 8 min; 35 cycles at 94°C for 30 sec, 58°C for 30 sec, and 72°C for 1 min; and an extension step of 72°C for 5 min.

Purification:

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: .. Purified 2nd PCR products were amplified using ExTaq Hot Start Version (Takara Bio) with the same conditions as for the 1st PCR methods. ..

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: Briefly, purified‐miRNAs were treated with Bacterial Alkaline Phosphatase to dephosphorylate the 3′ end of miRNAs at 37°C for 1 h, then purified and precipitated by standard phenol/chloroform extraction and ethanol precipitation methods. .. Finally, miRNAs were transcribed to cDNA and amplified using ExTaq Hot Start Version (Takara Bio).

Article Title: Historical Changes in the Ecosystem Condition of a Small Mountain Lake over the Past 60 Years as Revealed by Plankton Remains and Daphnia Ephippial Carapaces Stored in Lake Sediments
Article Snippet: PCR amplification of 12S rDNA fragments was carried out according to protocol III (using Type-it Microsatellite PCR Kit (Qiagen) and ExTaq Hot Start Version (Takara)) described in Ishida et al. [ ] with the primer set composed of d5f and d7r. .. DNA sequencing reaction was performed using a BigDye Terminator v3.1 Cycle Sequencing Ready Reaction Kit (Applied Biosystems) and each PCR product purified by CleanSEQ (Agencourt Bioscience).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Fc gamma receptor IIb participates in maternal IgG trafficking of human placental endothelial cells
Article Snippet: .. For conventional RT-PCR, ExTaq Hot Start Version (Takara Bio) was used for amplification using gene-specific primers, some of which were designed using DNASIS software (Hitachi, Tokyo, Japan). ..

Staining:

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: Second PCR products (approximately 65 bp) were separated by electrophoresis on a 10% TBE gel stained with SYBR Gold nucleic acid gel stain (Invitrogen, Carlsbad, CA, USA). .. Purified 2nd PCR products were amplified using ExTaq Hot Start Version (Takara Bio) with the same conditions as for the 1st PCR methods.

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: Finally, miRNAs were transcribed to cDNA and amplified using ExTaq Hot Start Version (Takara Bio). .. Second PCR products (approximately 65 bp) were separated by electrophoresis on a 10% TBE gel stained with SYBR Gold nucleic acid gel stain (Invitrogen, Carlsbad, CA, USA).

Nested PCR:

Article Title: Lack of a significant impact of Gag-Protease-mediated HIV-1 replication capacity on clinical parameters in treatment-naive Japanese individuals
Article Snippet: .. Plasma-derived gag -protease DNA fragments were generated from the cDNAs by nested PCR, using TaKaRa ExTaq Hot Start Version (Takara Bio Inc., Shiga, Japan). .. Chimeric HIV-1NL4-3 encoding plasma-derived gag -protease was generated by electroporating gag -protease PCR fragments with linearized gag -protease -deficient pNL4-3 (delta gag -pro NL4-3) into a CEM-derived GFP-reporter T-cell line engineered to carry LTR- G FP, C X CR4, and CC R 5 (CEM-GXR, a generous gift from T. Miura and M.A.

Article Title: The tRNA-Derived Small RNAs Regulate Gene Expression through Triggering Sequence-Specific Degradation of Target Transcripts in the Oomycete Pathogen Phytophthora sojae
Article Snippet: .. A nested PCR with ExTaq Hot Start Version (TaKaRa, China) was used to detect the degraded products with primers RA44OP/IP and GSP1/GSP2. .. The amplicons were further confirmed by sequencing.

Article Title: Direct detection of falciparum and non-falciparum malaria DNA from a drop of blood with high sensitivity by the dried-LAMP system
Article Snippet: Paragraph title: Plasmodium nested PCR ... All PCR reactions were carried out with ExTaq Hot Start Version (Takara-Bio, Shiga, Japan) in a volume of 20 μl.

Rapid Amplification of cDNA Ends:

Article Title: The tRNA-Derived Small RNAs Regulate Gene Expression through Triggering Sequence-Specific Degradation of Target Transcripts in the Oomycete Pathogen Phytophthora sojae
Article Snippet: 5′ RLM-RACE Analysis To perform 5′ RNA ligase -mediated rapid amplification of cDNA ends (5′ RLM-RACE), the poly (A)+ RNA was isolated by using Magnetic mRNA Isolation Kit (NEB, UK) from 200 μg total RNA. .. A nested PCR with ExTaq Hot Start Version (TaKaRa, China) was used to detect the degraded products with primers RA44OP/IP and GSP1/GSP2.

Plasmid Preparation:

Article Title: Direct detection of falciparum and non-falciparum malaria DNA from a drop of blood with high sensitivity by the dried-LAMP system
Article Snippet: All PCR reactions were carried out with ExTaq Hot Start Version (Takara-Bio, Shiga, Japan) in a volume of 20 μl. .. In case of detection of mixed wave patterns in the DNA sequencing, the amplicon was cloned into pGEM-T Easy vector (Promega, USA), and then several clones were sequenced.

Software:

Article Title: Fc gamma receptor IIb participates in maternal IgG trafficking of human placental endothelial cells
Article Snippet: .. For conventional RT-PCR, ExTaq Hot Start Version (Takara Bio) was used for amplification using gene-specific primers, some of which were designed using DNASIS software (Hitachi, Tokyo, Japan). ..

Agarose Gel Electrophoresis:

Article Title: Recurrent alterations of the WW domain containing oxidoreductase gene spanning the common fragile site FRA16D in multiple myeloma and monoclonal gammopathy of undetermined significance
Article Snippet: Each reaction (20 µl each) contained 0.2 µmol of each primer, 2.0 mM MgCl2 , 0.2 mM dNTP mix, 1X PCR buffer and 1.25 units of Takara ExTaq Hot Start Version (Takara Bio, Inc.). .. The amplicons were electrophoresed through a 2% agarose gel and visualized using ethidium bromide.

In Vitro:

Article Title: Lack of a significant impact of Gag-Protease-mediated HIV-1 replication capacity on clinical parameters in treatment-naive Japanese individuals
Article Snippet: Paragraph title: Generation of chimeric viruses and in vitro replication assays ... Plasma-derived gag -protease DNA fragments were generated from the cDNAs by nested PCR, using TaKaRa ExTaq Hot Start Version (Takara Bio Inc., Shiga, Japan).

Ethanol Precipitation:

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: Briefly, purified‐miRNAs were treated with Bacterial Alkaline Phosphatase to dephosphorylate the 3′ end of miRNAs at 37°C for 1 h, then purified and precipitated by standard phenol/chloroform extraction and ethanol precipitation methods. .. Finally, miRNAs were transcribed to cDNA and amplified using ExTaq Hot Start Version (Takara Bio).

Alkaline Lysis:

Article Title: Historical Changes in the Ecosystem Condition of a Small Mountain Lake over the Past 60 Years as Revealed by Plankton Remains and Daphnia Ephippial Carapaces Stored in Lake Sediments
Article Snippet: In short, regardless of intact of the resting eggs, ECs were individually transferred into a 0.2 ml tube containing 50 μl of alkaline lysis buffer (25 mM NaOH and 0.2 mM of disodium EDTA, pH 12) and subjected to five cycles of thermal shock consisting of 5 min. at −80°C and 20 sec. at 70°C. .. PCR amplification of 12S rDNA fragments was carried out according to protocol III (using Type-it Microsatellite PCR Kit (Qiagen) and ExTaq Hot Start Version (Takara)) described in Ishida et al. [ ] with the primer set composed of d5f and d7r.

High Throughput Screening Assay:

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: Paragraph title: MiRNA library construction and high‐throughput whole‐miRNAs sequencing ... Purified 2nd PCR products were amplified using ExTaq Hot Start Version (Takara Bio) with the same conditions as for the 1st PCR methods.

Gel Extraction:

Article Title: Targeting miR‐223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds
Article Snippet: Second PCR products were extracted from the gel and purified using a small RNA gel Extraction kit (Takara Bio) according to the manufacturer's instructions. .. Purified 2nd PCR products were amplified using ExTaq Hot Start Version (Takara Bio) with the same conditions as for the 1st PCR methods.

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  • 99
    TaKaRa extaq hot start version
    Extaq Hot Start Version, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/extaq hot start version/product/TaKaRa
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    extaq hot start version - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    TaKaRa extaq polymerase hot start version
    Extaq Polymerase Hot Start Version, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/extaq polymerase hot start version/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    extaq polymerase hot start version - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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