expression vector ptrchis 2c  (Thermo Fisher)


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    Name:
    pTrcHis A B C Bacterial Expression Vectors
    Description:
    Our pTrcHis A B C vectors are designed to offer enhanced translation initiation and high level expression in E coli These vectors feature • High level regulated transcription from the trc promoter • Enhanced translation efficiency of eukaryotic genes in E coli • The lacO operator and lacIq repressor gene for transcriptional regulation in any E coli strain This particular vector offers • N terminal polyhistidine 6xHis tag for rapid purification with nickel chelating resin and detection with an anti HisG antibody • N terminal Xpress epitope for easy detection with an anti Xpress antibody • Enterokinase cleavage site for removal of fusion tag For C terminal polyhistidine tag and c myc epitope please see our pTrcHis2 A B C Vector
    Catalog Number:
    v36020
    Price:
    None
    Applications:
    Bacterial Expression|Cloning|Destination Vectors|Gateway Cloning|Protein Biology|Protein Expression|pTrc and pBAD Regulated Bacterial Expression
    Category:
    DNA Vectors Clones Purified Nucleic Acids Libraries
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    Structured Review

    Thermo Fisher expression vector ptrchis 2c
    Our pTrcHis A B C vectors are designed to offer enhanced translation initiation and high level expression in E coli These vectors feature • High level regulated transcription from the trc promoter • Enhanced translation efficiency of eukaryotic genes in E coli • The lacO operator and lacIq repressor gene for transcriptional regulation in any E coli strain This particular vector offers • N terminal polyhistidine 6xHis tag for rapid purification with nickel chelating resin and detection with an anti HisG antibody • N terminal Xpress epitope for easy detection with an anti Xpress antibody • Enterokinase cleavage site for removal of fusion tag For C terminal polyhistidine tag and c myc epitope please see our pTrcHis2 A B C Vector
    https://www.bioz.com/result/expression vector ptrchis 2c/product/Thermo Fisher
    Average 94 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    expression vector ptrchis 2c - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Identification of Major Histocompatibility Complex Class II-Restricted Antigens and Epitopes of the Epstein-Barr Virus by a Novel Bacterial Expression Cloning Approach
    Article Snippet: .. To generate the expression vector, the open reading frame of chloramphenicol acetyltransferase (CAT) was cloned into the bacterial expression vector pTrcHisA (Invitrogen) to yield plasmid CAT-pTrcHisA. .. Using site-directed mutagenesis, the MscI site within the CAT ORF was destroyed without altering the amino acid sequence, giving rise to plasmid CAT-pTrcHisA M ̂ sc.

    Article Title: New vehicles allow detergent-free mass spectrometry of membrane protein complexes
    Article Snippet: .. Diaceylglycerol Kinase (DgkA) Expression/Purification The dgkA gene was synthesised (Genscript, Piscataway, NJ) based on the DNA sequence of dgkA in E. coli K12 and was cloned into pTrcHisB (Cat. V360-20, Invitrogen, Carlsbad, CA) using NcoI and EcoRI. .. The amino acid sequence of the expressed, wild-type (WT) protein is the same as that reported for pSD0005 , where the N-terminal methionine is replaced by a hexa-His tag-containing decapeptide (MGHHHHHHEL) to facilitate purification.

    Article Title: Host Lipid and Temperature as Important Screening Variables for Crystallizing Integral Membrane Proteins in Lipidic Mesophases. Trials with Diacylglycerol Kinase
    Article Snippet: .. The dgk A gene was synthesised (Genscript, Piscataway, NJ) based on the DNA sequence of dgk A in E. coli K12 and was cloned into pTrcHisB (Cat. V360-20, Invitrogen, Carlsbad , CA) using Nco I and EcoR I. .. The amino acid sequence of the expressed, wild-type (WT) protein is the same as that reported for pSD0005 by Bowie, where the N-terminal methionine is replaced by a hexa-His tag-containing decapeptide (MGHHHHHHEL) to facilitate purification.

    Article Title: A Tetratricopeptide Repeat Domain Protein has Profound Effects on Assembly of Periplasmic Flagella, Morphology, and Motility of the Lyme disease spirochete Borrelia burgdorferi
    Article Snippet: .. Briefly, B. burgdorferi FlbB was cloned in the expression vector pTrcHis-TOPO (Invitrogen) after removing the transmembrane binding domain (a.a. 1–50). .. E. coli codon plus cells harboring pTrcHis-TOPO:: flbB was expressed and purified according to the manufacturer’s protocol (Invitrogen).

    Article Title: PED/PEA-15 interacts with the 67 kD laminin receptor and regulates cell adhesion, migration, proliferation and apoptosis
    Article Snippet: .. To this end, wild-type 37LRP cDNA (33) was cloned into the pTrcHis B expression vector (Invitrogen, San Diego, CA, USA) and expressed in TOP-10 bacteria (Invitrogen). .. According to the procedures specified by Invitrogen, transformed bacteria were lysed in a denaturing lysis buffer (20 mM sodium phosphate, 500 mM sodium chloride, pH 7.8) containing 6M guanidium and His-tagged 37LRP (His-37LRP) was bound to nickel-NTA agarose beads, through its His-tagged N-terminus, in the same denaturing buffer containing 8M urea.

    Amplification:

    Article Title: An evolutionary perspective on the role of mesencephalic astrocyte-derived neurotrophic factor (MANF): At the crossroads of poriferan innate immune and apoptotic pathways
    Article Snippet: .. The amplicon, then, was inserted into the bacterial expression vector pTrcHis2 (Life Technologies). .. Following transformation of chemically competent One Shot BL21-AI or TOP10 Escherichia coli cells, the recombinant proteins with their vector-encoded N-terminal (pDEST17) or C-terminal (pTrcHis2) 6x His-tag were extracted and purified by immobilized metal affinity chromatography (IMAC), using the Profinia protein purification system (Bio-Rad, München, Germany) with a nickel-nitrilotriacetic acid (Ni-NTA) column.

    Agarose Gel Electrophoresis:

    Article Title: Peptides from American alligator plasma are antimicrobial against multi-drug resistant bacterial pathogens including Acinetobacter baumannii
    Article Snippet: .. Briefly, 200 ng of plasmid DNA (pTrcHis, Invitrogen V36020) was incubated with increasing concentrations of peptides in 20 μl of binding buffer (5 % glycerol, 10 mM Tris–HCl [pH 8.0], 1 mM EDTA, 1 mM DTT, 20 mM KCl and 50 μg/ml BSA) at room temperature for 20 min and subjected to electrophoresis on a 1.0 % agarose gel. .. DNA bands were visualized by ethidium bromide staining.

    Electrophoresis:

    Article Title: Peptides from American alligator plasma are antimicrobial against multi-drug resistant bacterial pathogens including Acinetobacter baumannii
    Article Snippet: .. Briefly, 200 ng of plasmid DNA (pTrcHis, Invitrogen V36020) was incubated with increasing concentrations of peptides in 20 μl of binding buffer (5 % glycerol, 10 mM Tris–HCl [pH 8.0], 1 mM EDTA, 1 mM DTT, 20 mM KCl and 50 μg/ml BSA) at room temperature for 20 min and subjected to electrophoresis on a 1.0 % agarose gel. .. DNA bands were visualized by ethidium bromide staining.

    Incubation:

    Article Title: Peptides from American alligator plasma are antimicrobial against multi-drug resistant bacterial pathogens including Acinetobacter baumannii
    Article Snippet: .. Briefly, 200 ng of plasmid DNA (pTrcHis, Invitrogen V36020) was incubated with increasing concentrations of peptides in 20 μl of binding buffer (5 % glycerol, 10 mM Tris–HCl [pH 8.0], 1 mM EDTA, 1 mM DTT, 20 mM KCl and 50 μg/ml BSA) at room temperature for 20 min and subjected to electrophoresis on a 1.0 % agarose gel. .. DNA bands were visualized by ethidium bromide staining.

    Expressing:

    Article Title: Identification of Major Histocompatibility Complex Class II-Restricted Antigens and Epitopes of the Epstein-Barr Virus by a Novel Bacterial Expression Cloning Approach
    Article Snippet: .. To generate the expression vector, the open reading frame of chloramphenicol acetyltransferase (CAT) was cloned into the bacterial expression vector pTrcHisA (Invitrogen) to yield plasmid CAT-pTrcHisA. .. Using site-directed mutagenesis, the MscI site within the CAT ORF was destroyed without altering the amino acid sequence, giving rise to plasmid CAT-pTrcHisA M ̂ sc.

    Article Title: New vehicles allow detergent-free mass spectrometry of membrane protein complexes
    Article Snippet: .. Diaceylglycerol Kinase (DgkA) Expression/Purification The dgkA gene was synthesised (Genscript, Piscataway, NJ) based on the DNA sequence of dgkA in E. coli K12 and was cloned into pTrcHisB (Cat. V360-20, Invitrogen, Carlsbad, CA) using NcoI and EcoRI. .. The amino acid sequence of the expressed, wild-type (WT) protein is the same as that reported for pSD0005 , where the N-terminal methionine is replaced by a hexa-His tag-containing decapeptide (MGHHHHHHEL) to facilitate purification.

    Article Title: Phosphorylation of the Synaptic Protein Interaction Site on N-type Calcium Channels Inhibits Interactions with SNARE Proteins
    Article Snippet: .. Recombinant DNA segments encoding the synprint region from the rat N-type calcium channel α1B subunit, designated α1B (718–859), α1B (832–963), and α1B (718–963), were subcloned into the bacterial expression vector pTrcHis C (Invitrogen, San Diego, CA) as described ( ). .. Recombinant rat cDNA for both syntaxin 1A and SNAP-25 were subcloned into the pGEX-4T bacterial expression vector (Pharmacia, Piscataway, NJ) as described ( , ; ).

    Article Title: An evolutionary perspective on the role of mesencephalic astrocyte-derived neurotrophic factor (MANF): At the crossroads of poriferan innate immune and apoptotic pathways
    Article Snippet: .. The amplicon, then, was inserted into the bacterial expression vector pTrcHis2 (Life Technologies). .. Following transformation of chemically competent One Shot BL21-AI or TOP10 Escherichia coli cells, the recombinant proteins with their vector-encoded N-terminal (pDEST17) or C-terminal (pTrcHis2) 6x His-tag were extracted and purified by immobilized metal affinity chromatography (IMAC), using the Profinia protein purification system (Bio-Rad, München, Germany) with a nickel-nitrilotriacetic acid (Ni-NTA) column.

    Article Title: A Tetratricopeptide Repeat Domain Protein has Profound Effects on Assembly of Periplasmic Flagella, Morphology, and Motility of the Lyme disease spirochete Borrelia burgdorferi
    Article Snippet: .. Briefly, B. burgdorferi FlbB was cloned in the expression vector pTrcHis-TOPO (Invitrogen) after removing the transmembrane binding domain (a.a. 1–50). .. E. coli codon plus cells harboring pTrcHis-TOPO:: flbB was expressed and purified according to the manufacturer’s protocol (Invitrogen).

    Article Title: PED/PEA-15 interacts with the 67 kD laminin receptor and regulates cell adhesion, migration, proliferation and apoptosis
    Article Snippet: .. To this end, wild-type 37LRP cDNA (33) was cloned into the pTrcHis B expression vector (Invitrogen, San Diego, CA, USA) and expressed in TOP-10 bacteria (Invitrogen). .. According to the procedures specified by Invitrogen, transformed bacteria were lysed in a denaturing lysis buffer (20 mM sodium phosphate, 500 mM sodium chloride, pH 7.8) containing 6M guanidium and His-tagged 37LRP (His-37LRP) was bound to nickel-NTA agarose beads, through its His-tagged N-terminus, in the same denaturing buffer containing 8M urea.

    Sequencing:

    Article Title: New vehicles allow detergent-free mass spectrometry of membrane protein complexes
    Article Snippet: .. Diaceylglycerol Kinase (DgkA) Expression/Purification The dgkA gene was synthesised (Genscript, Piscataway, NJ) based on the DNA sequence of dgkA in E. coli K12 and was cloned into pTrcHisB (Cat. V360-20, Invitrogen, Carlsbad, CA) using NcoI and EcoRI. .. The amino acid sequence of the expressed, wild-type (WT) protein is the same as that reported for pSD0005 , where the N-terminal methionine is replaced by a hexa-His tag-containing decapeptide (MGHHHHHHEL) to facilitate purification.

    Article Title: Host Lipid and Temperature as Important Screening Variables for Crystallizing Integral Membrane Proteins in Lipidic Mesophases. Trials with Diacylglycerol Kinase
    Article Snippet: .. The dgk A gene was synthesised (Genscript, Piscataway, NJ) based on the DNA sequence of dgk A in E. coli K12 and was cloned into pTrcHisB (Cat. V360-20, Invitrogen, Carlsbad , CA) using Nco I and EcoR I. .. The amino acid sequence of the expressed, wild-type (WT) protein is the same as that reported for pSD0005 by Bowie, where the N-terminal methionine is replaced by a hexa-His tag-containing decapeptide (MGHHHHHHEL) to facilitate purification.

    Recombinant:

    Article Title: Phosphorylation of the Synaptic Protein Interaction Site on N-type Calcium Channels Inhibits Interactions with SNARE Proteins
    Article Snippet: .. Recombinant DNA segments encoding the synprint region from the rat N-type calcium channel α1B subunit, designated α1B (718–859), α1B (832–963), and α1B (718–963), were subcloned into the bacterial expression vector pTrcHis C (Invitrogen, San Diego, CA) as described ( ). .. Recombinant rat cDNA for both syntaxin 1A and SNAP-25 were subcloned into the pGEX-4T bacterial expression vector (Pharmacia, Piscataway, NJ) as described ( , ; ).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Identification of Major Histocompatibility Complex Class II-Restricted Antigens and Epitopes of the Epstein-Barr Virus by a Novel Bacterial Expression Cloning Approach
    Article Snippet: .. To generate the expression vector, the open reading frame of chloramphenicol acetyltransferase (CAT) was cloned into the bacterial expression vector pTrcHisA (Invitrogen) to yield plasmid CAT-pTrcHisA. .. Using site-directed mutagenesis, the MscI site within the CAT ORF was destroyed without altering the amino acid sequence, giving rise to plasmid CAT-pTrcHisA M ̂ sc.

    Binding Assay:

    Article Title: Peptides from American alligator plasma are antimicrobial against multi-drug resistant bacterial pathogens including Acinetobacter baumannii
    Article Snippet: .. Briefly, 200 ng of plasmid DNA (pTrcHis, Invitrogen V36020) was incubated with increasing concentrations of peptides in 20 μl of binding buffer (5 % glycerol, 10 mM Tris–HCl [pH 8.0], 1 mM EDTA, 1 mM DTT, 20 mM KCl and 50 μg/ml BSA) at room temperature for 20 min and subjected to electrophoresis on a 1.0 % agarose gel. .. DNA bands were visualized by ethidium bromide staining.

    Article Title: A Tetratricopeptide Repeat Domain Protein has Profound Effects on Assembly of Periplasmic Flagella, Morphology, and Motility of the Lyme disease spirochete Borrelia burgdorferi
    Article Snippet: .. Briefly, B. burgdorferi FlbB was cloned in the expression vector pTrcHis-TOPO (Invitrogen) after removing the transmembrane binding domain (a.a. 1–50). .. E. coli codon plus cells harboring pTrcHis-TOPO:: flbB was expressed and purified according to the manufacturer’s protocol (Invitrogen).

    Plasmid Preparation:

    Article Title: Identification of Major Histocompatibility Complex Class II-Restricted Antigens and Epitopes of the Epstein-Barr Virus by a Novel Bacterial Expression Cloning Approach
    Article Snippet: .. To generate the expression vector, the open reading frame of chloramphenicol acetyltransferase (CAT) was cloned into the bacterial expression vector pTrcHisA (Invitrogen) to yield plasmid CAT-pTrcHisA. .. Using site-directed mutagenesis, the MscI site within the CAT ORF was destroyed without altering the amino acid sequence, giving rise to plasmid CAT-pTrcHisA M ̂ sc.

    Article Title: Phosphorylation of the Synaptic Protein Interaction Site on N-type Calcium Channels Inhibits Interactions with SNARE Proteins
    Article Snippet: .. Recombinant DNA segments encoding the synprint region from the rat N-type calcium channel α1B subunit, designated α1B (718–859), α1B (832–963), and α1B (718–963), were subcloned into the bacterial expression vector pTrcHis C (Invitrogen, San Diego, CA) as described ( ). .. Recombinant rat cDNA for both syntaxin 1A and SNAP-25 were subcloned into the pGEX-4T bacterial expression vector (Pharmacia, Piscataway, NJ) as described ( , ; ).

    Article Title: Peptides from American alligator plasma are antimicrobial against multi-drug resistant bacterial pathogens including Acinetobacter baumannii
    Article Snippet: .. Briefly, 200 ng of plasmid DNA (pTrcHis, Invitrogen V36020) was incubated with increasing concentrations of peptides in 20 μl of binding buffer (5 % glycerol, 10 mM Tris–HCl [pH 8.0], 1 mM EDTA, 1 mM DTT, 20 mM KCl and 50 μg/ml BSA) at room temperature for 20 min and subjected to electrophoresis on a 1.0 % agarose gel. .. DNA bands were visualized by ethidium bromide staining.

    Article Title: An evolutionary perspective on the role of mesencephalic astrocyte-derived neurotrophic factor (MANF): At the crossroads of poriferan innate immune and apoptotic pathways
    Article Snippet: .. The amplicon, then, was inserted into the bacterial expression vector pTrcHis2 (Life Technologies). .. Following transformation of chemically competent One Shot BL21-AI or TOP10 Escherichia coli cells, the recombinant proteins with their vector-encoded N-terminal (pDEST17) or C-terminal (pTrcHis2) 6x His-tag were extracted and purified by immobilized metal affinity chromatography (IMAC), using the Profinia protein purification system (Bio-Rad, München, Germany) with a nickel-nitrilotriacetic acid (Ni-NTA) column.

    Article Title: A Tetratricopeptide Repeat Domain Protein has Profound Effects on Assembly of Periplasmic Flagella, Morphology, and Motility of the Lyme disease spirochete Borrelia burgdorferi
    Article Snippet: .. Briefly, B. burgdorferi FlbB was cloned in the expression vector pTrcHis-TOPO (Invitrogen) after removing the transmembrane binding domain (a.a. 1–50). .. E. coli codon plus cells harboring pTrcHis-TOPO:: flbB was expressed and purified according to the manufacturer’s protocol (Invitrogen).