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Amaxa expression vector pmax gfp
Expression Vector Pmax Gfp, supplied by Amaxa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expression vector pmax gfp/product/Amaxa
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
expression vector pmax gfp - by Bioz Stars, 2020-08
85/100 stars

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Related Articles

Transfection:

Article Title: Schwann Cell Dedifferentiation Is Independent of Mitogenic Signaling and Uncoupled to Proliferation
Article Snippet: .. The percentage of transfected SCs was 20–30% based on GFP fluorescence from the expression vector pMAX-GFP (Amaxa Biosystems), which was routinely used as a positive control to estimate transfection efficiency and to make the total amount of transfected DNA equivalent in all conditions. ..

Article Title: Protein Kinase A-mediated Gating of Neuregulin-dependent ErbB2-ErbB3 Activation Underlies the Synergistic Action of cAMP on Schwann Cell Proliferation *Protein Kinase A-mediated Gating of Neuregulin-dependent ErbB2-ErbB3 Activation Underlies the Synergistic Action of cAMP on Schwann Cell Proliferation * S⃞
Article Snippet: .. Transfection efficiency for SCs was 40–60% based on green fluorescent protein fluorescence from the expression vector pMAX-green fluorescent protein (Amaxa Biosystems), which was routinely used as a positive control to estimate transfection efficiency. .. Western Blots —Protein samples from total cell lysates were prepared by resuspending the cells in lysis buffer (50 m m Tris, 150 m m NaCl, 1% SDS, 0.5 m m dithiothreitol) containing protease inhibitors (1 m m phenylmethylsulfonyl fluoride, 20 μg/ml aprotinin, and 20 μg/ml leupeptin) and phosphatase inhibitors (phosphatase cocktails I and II, Sigma).

Fluorescence:

Article Title: Schwann Cell Dedifferentiation Is Independent of Mitogenic Signaling and Uncoupled to Proliferation
Article Snippet: .. The percentage of transfected SCs was 20–30% based on GFP fluorescence from the expression vector pMAX-GFP (Amaxa Biosystems), which was routinely used as a positive control to estimate transfection efficiency and to make the total amount of transfected DNA equivalent in all conditions. ..

Article Title: Protein Kinase A-mediated Gating of Neuregulin-dependent ErbB2-ErbB3 Activation Underlies the Synergistic Action of cAMP on Schwann Cell Proliferation *Protein Kinase A-mediated Gating of Neuregulin-dependent ErbB2-ErbB3 Activation Underlies the Synergistic Action of cAMP on Schwann Cell Proliferation * S⃞
Article Snippet: .. Transfection efficiency for SCs was 40–60% based on green fluorescent protein fluorescence from the expression vector pMAX-green fluorescent protein (Amaxa Biosystems), which was routinely used as a positive control to estimate transfection efficiency. .. Western Blots —Protein samples from total cell lysates were prepared by resuspending the cells in lysis buffer (50 m m Tris, 150 m m NaCl, 1% SDS, 0.5 m m dithiothreitol) containing protease inhibitors (1 m m phenylmethylsulfonyl fluoride, 20 μg/ml aprotinin, and 20 μg/ml leupeptin) and phosphatase inhibitors (phosphatase cocktails I and II, Sigma).

Positive Control:

Article Title: Schwann Cell Dedifferentiation Is Independent of Mitogenic Signaling and Uncoupled to Proliferation
Article Snippet: .. The percentage of transfected SCs was 20–30% based on GFP fluorescence from the expression vector pMAX-GFP (Amaxa Biosystems), which was routinely used as a positive control to estimate transfection efficiency and to make the total amount of transfected DNA equivalent in all conditions. ..

Article Title: Protein Kinase A-mediated Gating of Neuregulin-dependent ErbB2-ErbB3 Activation Underlies the Synergistic Action of cAMP on Schwann Cell Proliferation *Protein Kinase A-mediated Gating of Neuregulin-dependent ErbB2-ErbB3 Activation Underlies the Synergistic Action of cAMP on Schwann Cell Proliferation * S⃞
Article Snippet: .. Transfection efficiency for SCs was 40–60% based on green fluorescent protein fluorescence from the expression vector pMAX-green fluorescent protein (Amaxa Biosystems), which was routinely used as a positive control to estimate transfection efficiency. .. Western Blots —Protein samples from total cell lysates were prepared by resuspending the cells in lysis buffer (50 m m Tris, 150 m m NaCl, 1% SDS, 0.5 m m dithiothreitol) containing protease inhibitors (1 m m phenylmethylsulfonyl fluoride, 20 μg/ml aprotinin, and 20 μg/ml leupeptin) and phosphatase inhibitors (phosphatase cocktails I and II, Sigma).

Plasmid Preparation:

Article Title: Schwann Cell Dedifferentiation Is Independent of Mitogenic Signaling and Uncoupled to Proliferation
Article Snippet: .. The percentage of transfected SCs was 20–30% based on GFP fluorescence from the expression vector pMAX-GFP (Amaxa Biosystems), which was routinely used as a positive control to estimate transfection efficiency and to make the total amount of transfected DNA equivalent in all conditions. ..

Article Title: Protein Kinase A-mediated Gating of Neuregulin-dependent ErbB2-ErbB3 Activation Underlies the Synergistic Action of cAMP on Schwann Cell Proliferation *Protein Kinase A-mediated Gating of Neuregulin-dependent ErbB2-ErbB3 Activation Underlies the Synergistic Action of cAMP on Schwann Cell Proliferation * S⃞
Article Snippet: .. Transfection efficiency for SCs was 40–60% based on green fluorescent protein fluorescence from the expression vector pMAX-green fluorescent protein (Amaxa Biosystems), which was routinely used as a positive control to estimate transfection efficiency. .. Western Blots —Protein samples from total cell lysates were prepared by resuspending the cells in lysis buffer (50 m m Tris, 150 m m NaCl, 1% SDS, 0.5 m m dithiothreitol) containing protease inhibitors (1 m m phenylmethylsulfonyl fluoride, 20 μg/ml aprotinin, and 20 μg/ml leupeptin) and phosphatase inhibitors (phosphatase cocktails I and II, Sigma).

Expressing:

Article Title: Schwann Cell Dedifferentiation Is Independent of Mitogenic Signaling and Uncoupled to Proliferation
Article Snippet: .. The percentage of transfected SCs was 20–30% based on GFP fluorescence from the expression vector pMAX-GFP (Amaxa Biosystems), which was routinely used as a positive control to estimate transfection efficiency and to make the total amount of transfected DNA equivalent in all conditions. ..

Article Title: Protein Kinase A-mediated Gating of Neuregulin-dependent ErbB2-ErbB3 Activation Underlies the Synergistic Action of cAMP on Schwann Cell Proliferation *Protein Kinase A-mediated Gating of Neuregulin-dependent ErbB2-ErbB3 Activation Underlies the Synergistic Action of cAMP on Schwann Cell Proliferation * S⃞
Article Snippet: .. Transfection efficiency for SCs was 40–60% based on green fluorescent protein fluorescence from the expression vector pMAX-green fluorescent protein (Amaxa Biosystems), which was routinely used as a positive control to estimate transfection efficiency. .. Western Blots —Protein samples from total cell lysates were prepared by resuspending the cells in lysis buffer (50 m m Tris, 150 m m NaCl, 1% SDS, 0.5 m m dithiothreitol) containing protease inhibitors (1 m m phenylmethylsulfonyl fluoride, 20 μg/ml aprotinin, and 20 μg/ml leupeptin) and phosphatase inhibitors (phosphatase cocktails I and II, Sigma).

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  • 85
    Amaxa expression vector pmax gfp
    Expression Vector Pmax Gfp, supplied by Amaxa, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression vector pmax gfp/product/Amaxa
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    expression vector pmax gfp - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    93
    Amaxa pmax gfp mammalian expression vector
    Pmax Gfp Mammalian Expression Vector, supplied by Amaxa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmax gfp mammalian expression vector/product/Amaxa
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmax gfp mammalian expression vector - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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