expanded high fidelity pcr system  (Roche)


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    Structured Review

    Roche expanded high fidelity pcr system
    Expanded High Fidelity Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expanded high fidelity pcr system/product/Roche
    Average 88 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    expanded high fidelity pcr system - by Bioz Stars, 2020-08
    88/100 stars

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    Related Articles

    Amplification:

    Article Title: Deficient for endoplasmic reticulum calcium sensors Stim1 and Stim2 affects aberrant antibody affinity maturation in B cells
    Article Snippet: .. Genomic DNA from 104 cell equivalents were subjected to two round of genomic PCR amplification protocol using the Expanded High Fidelity PCR system (Roche, Nutley, NJ) and primers previously described G. Esposito et al., Proc Natl Acad Sci U S A 97, 1166 (Feb 1, 2000). .. PCR fragments corresponding to the Vh186.2 gene were cloned into the pGEM-T easy (Promega) and sequenced using Sanger sequencing.

    Article Title: Evaluation of All Nonsynonymous Single-Nucleotide Polymorphisms in the Gene Encoding Human Deoxyribonuclease I-Like 1, Possibly Implicated in the Blocking of Endocytosis-Mediated Foreign Gene Transfer
    Article Snippet: .. Expression pcDNA3.1 (+) vectors (Invitrogen, San Diego, CA) inserted with the entire coding sequence of human DNase 1L1 cDNA was prepared from the total RNA of human skeleton muscle (Clontech, Palo Alta, CA) by reverse transcriptase–PCR amplification using an Expanded High Fidelity PCR System (Roche Diagnosis, Mannheim, Germany) with a set of two primers, 5′-GAATTCCAGCCACACAGCCATGCACTACC-3′ (sense) and 5′-CTCGAGCGCTCAGGCAGCAGGGCACAG-3′ (antisense), corresponding to the sequences of cDNA from positions −13–10 and 900–932, respectively; inserted cDNAs was derived from the predominant haplotype of the SNPs in Japanese. .. Using this construct as a template, the DNase 1L1 ΔC construct, which was devoid of the nucleotide region corresponding to the C-terminal domain, including Ala277–Ala302, used for the activity assay was prepared using the KOD-Plus Mutagenesis kit (Toyobo Co., Ltd., Osaka, Japan) and used as a wild type.

    Article Title: Cloning and characterization of feline islet glucokinase
    Article Snippet: .. PCR amplification was done by touchdown PCR using the Expanded High Fidelity PCR System (Roche Applied Science) and the TGradient Thermocycler (Biometra). ..

    Article Title: Survey of single-nucleotide polymorphisms in the gene encoding human deoxyribonuclease I-like 2 producing loss of function potentially implicated in the pathogenesis of parakeratosis
    Article Snippet: .. A DNA fragment containing the entire coding sequence of human DNase 1L2 cDNA was prepared by reverse transcription (RT)-PCR amplification of the total RNA of human peripheral leukocytes (Clontech, Palo Alta, CA, USA) using an Expanded High Fidelity PCR System (Roche Diagnosis, Mannheim, Germany); a set of two primers, 5'- GGATCC CACCCACATCCAAGGCGGCAGCG-3' (sense) and 5'- CTCGAG TCATCGGTGGAACTTGAG-3' (antisense), corresponding to the sequences of positions -54–-31and 883–907 in the DNase 1L2 cDNA, in which the restriction site of Eco RI and Xho I as shown in bold were introduced, respectively, were used. .. After digested with Eco RI and Xho I, the amplified DNA fragment was ligated into pcDNA3.1(+) (Invitrogen, San Diego, CA, USA).

    Article Title: GPR139 and Dopamine D2 Receptor Co-express in the Same Cells of the Brain and May Functionally Interact
    Article Snippet: .. Specific primers (Forward primer: 5’- atg tca GAA TTC GCC ACC atg gat cca ctg aat ctg tcc tgg tat gat-3’; Reverse primer: 5’- tga cat GCG GCC GCt cag cag tga agg atc ttc agg aag gcc ttg cgg aa-3’) designed based on published human DRD2 cDNA coding region (Genbank Accession NO: M30625.1) were used for PCR amplification of the human brain cDNA (Clontech) using an Expanded High Fidelity PCR System (Roche). .. The resulting PCR product was cloned into pcDNA3.1 between EcoR1 and Not1 sites (Promega, Madison, WI, United States).

    Polymerase Chain Reaction:

    Article Title: Deficient for endoplasmic reticulum calcium sensors Stim1 and Stim2 affects aberrant antibody affinity maturation in B cells
    Article Snippet: .. Genomic DNA from 104 cell equivalents were subjected to two round of genomic PCR amplification protocol using the Expanded High Fidelity PCR system (Roche, Nutley, NJ) and primers previously described G. Esposito et al., Proc Natl Acad Sci U S A 97, 1166 (Feb 1, 2000). .. PCR fragments corresponding to the Vh186.2 gene were cloned into the pGEM-T easy (Promega) and sequenced using Sanger sequencing.

    Article Title: Evaluation of All Nonsynonymous Single-Nucleotide Polymorphisms in the Gene Encoding Human Deoxyribonuclease I-Like 1, Possibly Implicated in the Blocking of Endocytosis-Mediated Foreign Gene Transfer
    Article Snippet: .. Expression pcDNA3.1 (+) vectors (Invitrogen, San Diego, CA) inserted with the entire coding sequence of human DNase 1L1 cDNA was prepared from the total RNA of human skeleton muscle (Clontech, Palo Alta, CA) by reverse transcriptase–PCR amplification using an Expanded High Fidelity PCR System (Roche Diagnosis, Mannheim, Germany) with a set of two primers, 5′-GAATTCCAGCCACACAGCCATGCACTACC-3′ (sense) and 5′-CTCGAGCGCTCAGGCAGCAGGGCACAG-3′ (antisense), corresponding to the sequences of cDNA from positions −13–10 and 900–932, respectively; inserted cDNAs was derived from the predominant haplotype of the SNPs in Japanese. .. Using this construct as a template, the DNase 1L1 ΔC construct, which was devoid of the nucleotide region corresponding to the C-terminal domain, including Ala277–Ala302, used for the activity assay was prepared using the KOD-Plus Mutagenesis kit (Toyobo Co., Ltd., Osaka, Japan) and used as a wild type.

    Article Title: Cloning and characterization of feline islet glucokinase
    Article Snippet: .. PCR amplification was done by touchdown PCR using the Expanded High Fidelity PCR System (Roche Applied Science) and the TGradient Thermocycler (Biometra). ..

    Article Title: Survey of single-nucleotide polymorphisms in the gene encoding human deoxyribonuclease I-like 2 producing loss of function potentially implicated in the pathogenesis of parakeratosis
    Article Snippet: .. A DNA fragment containing the entire coding sequence of human DNase 1L2 cDNA was prepared by reverse transcription (RT)-PCR amplification of the total RNA of human peripheral leukocytes (Clontech, Palo Alta, CA, USA) using an Expanded High Fidelity PCR System (Roche Diagnosis, Mannheim, Germany); a set of two primers, 5'- GGATCC CACCCACATCCAAGGCGGCAGCG-3' (sense) and 5'- CTCGAG TCATCGGTGGAACTTGAG-3' (antisense), corresponding to the sequences of positions -54–-31and 883–907 in the DNase 1L2 cDNA, in which the restriction site of Eco RI and Xho I as shown in bold were introduced, respectively, were used. .. After digested with Eco RI and Xho I, the amplified DNA fragment was ligated into pcDNA3.1(+) (Invitrogen, San Diego, CA, USA).

    Article Title: Mutations in the M-Gene Segment Can Substantially Increase Replication Efficiency of NS1 Deletion Influenza A Virus in MDCK Cells
    Article Snippet: .. Universal influenza genome primer uni12 ( ) was used for reverse transcriptase reactions with the Superscript III first strand synthesis system (Invitrogen), followed by segment-specific PCRs with an Expanded high-fidelity PCR system (Roche Applied Science). .. DNA sequencing was performed at Baseclear (Leiden, Netherlands), and sequence analysis was done with Lasergene (DNASTAR Inc., WI).

    Article Title: Characterization of the Role of β-Carotene 9,10-Dioxygenase in Macular Pigment Metabolism *
    Article Snippet: .. All PCRs were carried out with the Expanded High Fidelity PCR system (Roche Applied Science). ..

    Article Title: GPR139 and Dopamine D2 Receptor Co-express in the Same Cells of the Brain and May Functionally Interact
    Article Snippet: .. Specific primers (Forward primer: 5’- atg tca GAA TTC GCC ACC atg gat cca ctg aat ctg tcc tgg tat gat-3’; Reverse primer: 5’- tga cat GCG GCC GCt cag cag tga agg atc ttc agg aag gcc ttg cgg aa-3’) designed based on published human DRD2 cDNA coding region (Genbank Accession NO: M30625.1) were used for PCR amplification of the human brain cDNA (Clontech) using an Expanded High Fidelity PCR System (Roche). .. The resulting PCR product was cloned into pcDNA3.1 between EcoR1 and Not1 sites (Promega, Madison, WI, United States).

    Article Title: Evidence for compartmentalization of mammalian carotenoid metabolism
    Article Snippet: .. All PCRs were carried out with the Expanded High Fidelity PCR system (Roche, Indianapolis, IN, USA). ..

    Sequencing:

    Article Title: Evaluation of All Nonsynonymous Single-Nucleotide Polymorphisms in the Gene Encoding Human Deoxyribonuclease I-Like 1, Possibly Implicated in the Blocking of Endocytosis-Mediated Foreign Gene Transfer
    Article Snippet: .. Expression pcDNA3.1 (+) vectors (Invitrogen, San Diego, CA) inserted with the entire coding sequence of human DNase 1L1 cDNA was prepared from the total RNA of human skeleton muscle (Clontech, Palo Alta, CA) by reverse transcriptase–PCR amplification using an Expanded High Fidelity PCR System (Roche Diagnosis, Mannheim, Germany) with a set of two primers, 5′-GAATTCCAGCCACACAGCCATGCACTACC-3′ (sense) and 5′-CTCGAGCGCTCAGGCAGCAGGGCACAG-3′ (antisense), corresponding to the sequences of cDNA from positions −13–10 and 900–932, respectively; inserted cDNAs was derived from the predominant haplotype of the SNPs in Japanese. .. Using this construct as a template, the DNase 1L1 ΔC construct, which was devoid of the nucleotide region corresponding to the C-terminal domain, including Ala277–Ala302, used for the activity assay was prepared using the KOD-Plus Mutagenesis kit (Toyobo Co., Ltd., Osaka, Japan) and used as a wild type.

    Article Title: Survey of single-nucleotide polymorphisms in the gene encoding human deoxyribonuclease I-like 2 producing loss of function potentially implicated in the pathogenesis of parakeratosis
    Article Snippet: .. A DNA fragment containing the entire coding sequence of human DNase 1L2 cDNA was prepared by reverse transcription (RT)-PCR amplification of the total RNA of human peripheral leukocytes (Clontech, Palo Alta, CA, USA) using an Expanded High Fidelity PCR System (Roche Diagnosis, Mannheim, Germany); a set of two primers, 5'- GGATCC CACCCACATCCAAGGCGGCAGCG-3' (sense) and 5'- CTCGAG TCATCGGTGGAACTTGAG-3' (antisense), corresponding to the sequences of positions -54–-31and 883–907 in the DNase 1L2 cDNA, in which the restriction site of Eco RI and Xho I as shown in bold were introduced, respectively, were used. .. After digested with Eco RI and Xho I, the amplified DNA fragment was ligated into pcDNA3.1(+) (Invitrogen, San Diego, CA, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Survey of single-nucleotide polymorphisms in the gene encoding human deoxyribonuclease I-like 2 producing loss of function potentially implicated in the pathogenesis of parakeratosis
    Article Snippet: .. A DNA fragment containing the entire coding sequence of human DNase 1L2 cDNA was prepared by reverse transcription (RT)-PCR amplification of the total RNA of human peripheral leukocytes (Clontech, Palo Alta, CA, USA) using an Expanded High Fidelity PCR System (Roche Diagnosis, Mannheim, Germany); a set of two primers, 5'- GGATCC CACCCACATCCAAGGCGGCAGCG-3' (sense) and 5'- CTCGAG TCATCGGTGGAACTTGAG-3' (antisense), corresponding to the sequences of positions -54–-31and 883–907 in the DNase 1L2 cDNA, in which the restriction site of Eco RI and Xho I as shown in bold were introduced, respectively, were used. .. After digested with Eco RI and Xho I, the amplified DNA fragment was ligated into pcDNA3.1(+) (Invitrogen, San Diego, CA, USA).

    Expressing:

    Article Title: Evaluation of All Nonsynonymous Single-Nucleotide Polymorphisms in the Gene Encoding Human Deoxyribonuclease I-Like 1, Possibly Implicated in the Blocking of Endocytosis-Mediated Foreign Gene Transfer
    Article Snippet: .. Expression pcDNA3.1 (+) vectors (Invitrogen, San Diego, CA) inserted with the entire coding sequence of human DNase 1L1 cDNA was prepared from the total RNA of human skeleton muscle (Clontech, Palo Alta, CA) by reverse transcriptase–PCR amplification using an Expanded High Fidelity PCR System (Roche Diagnosis, Mannheim, Germany) with a set of two primers, 5′-GAATTCCAGCCACACAGCCATGCACTACC-3′ (sense) and 5′-CTCGAGCGCTCAGGCAGCAGGGCACAG-3′ (antisense), corresponding to the sequences of cDNA from positions −13–10 and 900–932, respectively; inserted cDNAs was derived from the predominant haplotype of the SNPs in Japanese. .. Using this construct as a template, the DNase 1L1 ΔC construct, which was devoid of the nucleotide region corresponding to the C-terminal domain, including Ala277–Ala302, used for the activity assay was prepared using the KOD-Plus Mutagenesis kit (Toyobo Co., Ltd., Osaka, Japan) and used as a wild type.

    Touchdown PCR:

    Article Title: Cloning and characterization of feline islet glucokinase
    Article Snippet: .. PCR amplification was done by touchdown PCR using the Expanded High Fidelity PCR System (Roche Applied Science) and the TGradient Thermocycler (Biometra). ..

    CTG Assay:

    Article Title: GPR139 and Dopamine D2 Receptor Co-express in the Same Cells of the Brain and May Functionally Interact
    Article Snippet: .. Specific primers (Forward primer: 5’- atg tca GAA TTC GCC ACC atg gat cca ctg aat ctg tcc tgg tat gat-3’; Reverse primer: 5’- tga cat GCG GCC GCt cag cag tga agg atc ttc agg aag gcc ttg cgg aa-3’) designed based on published human DRD2 cDNA coding region (Genbank Accession NO: M30625.1) were used for PCR amplification of the human brain cDNA (Clontech) using an Expanded High Fidelity PCR System (Roche). .. The resulting PCR product was cloned into pcDNA3.1 between EcoR1 and Not1 sites (Promega, Madison, WI, United States).

    Derivative Assay:

    Article Title: Evaluation of All Nonsynonymous Single-Nucleotide Polymorphisms in the Gene Encoding Human Deoxyribonuclease I-Like 1, Possibly Implicated in the Blocking of Endocytosis-Mediated Foreign Gene Transfer
    Article Snippet: .. Expression pcDNA3.1 (+) vectors (Invitrogen, San Diego, CA) inserted with the entire coding sequence of human DNase 1L1 cDNA was prepared from the total RNA of human skeleton muscle (Clontech, Palo Alta, CA) by reverse transcriptase–PCR amplification using an Expanded High Fidelity PCR System (Roche Diagnosis, Mannheim, Germany) with a set of two primers, 5′-GAATTCCAGCCACACAGCCATGCACTACC-3′ (sense) and 5′-CTCGAGCGCTCAGGCAGCAGGGCACAG-3′ (antisense), corresponding to the sequences of cDNA from positions −13–10 and 900–932, respectively; inserted cDNAs was derived from the predominant haplotype of the SNPs in Japanese. .. Using this construct as a template, the DNase 1L1 ΔC construct, which was devoid of the nucleotide region corresponding to the C-terminal domain, including Ala277–Ala302, used for the activity assay was prepared using the KOD-Plus Mutagenesis kit (Toyobo Co., Ltd., Osaka, Japan) and used as a wild type.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Roche expanded high fidelity pcr system
    Expanded High Fidelity Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expanded high fidelity pcr system/product/Roche
    Average 88 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    expanded high fidelity pcr system - by Bioz Stars, 2020-08
    88/100 stars
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