primers kir3dl ex3 fw  (Roche)


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    Structured Review

    Roche primers kir3dl ex3 fw
    Primers Kir3dl Ex3 Fw, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primers kir3dl ex3 fw/product/Roche
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    primers kir3dl ex3 fw - by Bioz Stars, 2020-09
    85/100 stars

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    Amplification:

    Article Title: Natural Killer Cell Receptor Genes in the Family Equidae: Not only Ly49
    Article Snippet: .. First fragment covering exon3-exon5 (3329 bp) was amplified using primers kir3dl ex3 fw and kir3dl ex5 rev ( ) in Expand LongRange dNTPack system (Roche Diagnostics, Mannheim, Germany) with initial denaturation at 92°C for 2 min, followed by 10 cycles of 92°C for 10 s, annealing at 60°C–55°C (touchdown 0,5°C per cycle) for 15 s and elongation at 68°C for 5 min and 25 cycles of 92°C for 10 s, annealing for 15 s at 55°C and elongation at 68°C for 5 min/+20 s for each successive cycle with final elongation at 68°C for 7 min. Second fragment covering exon5–exon9 (5020 bp) was amplified by same protocol with both annealing temperatures at 50°C and primers in exon 5 (kir3dl ex5 fw) and 3′UTR (kir3dl rev). ..

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    Roche expand longrange dntpack system
    Expand Longrange Dntpack System, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand longrange dntpack system/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand longrange dntpack system - by Bioz Stars, 2020-09
    85/100 stars
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