expand long range dntpack  (Millipore)


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    Name:
    Expand Long Range dNTPack
    Description:
    The Expand Long Range dNTPack is the second generation version of the Expand Long Template PCR System optimized and designed to consistently amplify PCR products from 5kb up to 25kb from genomic DNA or up to 40kb from lambda DNA templates The supplied buffer can be used for all PCR product sizes In addition a buffer without MgCl2 and separate vials of MgCl2 and DMSO are included if optimization is needed Expand Long Range dNTPack contains a special enzyme mix that consists of thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity This powerful polymerase mixture produces a high yield of PCR product Due to the inherent 3 5 exonuclease activity of the proofreading polymerase Expand Long Range dNTPack copies DNA three times more accurately than Taq DNA Polymerase
    Catalog Number:
    elongn-ro
    Price:
    None
    Applications:
    The Expand Long Range dNTPack is optimized to efficiently amplify large genomic DNA fragments from 5kb to 25kb in combination with a threefold higher fidelity than Taq DNA Polymerase. The Expand Long Range dNTPack is ideally suited for: . Genome mapping and sequencing. Contig construction. Characterization of cloned sequences in lambda phages or cosmids. Cloning and analysis of eukaryotic genes. Rapid identification and cloning of complete genes from genomic DNA using cDNA sequence as starting material. Two-step RT-PCRUse the specially designed buffer set, ultrapure PCR Nucleotide Mix, and optimized blend of thermostable DNA polymerases for:. PCR. Large-fragment amplification. RT-PCRFor amplicon sizes of 20 to 35kb choose Expand 20kb PLUS PCR System, dNTPack.
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    Structured Review

    Millipore expand long range dntpack
    Expand Long Range dNTPack
    The Expand Long Range dNTPack is the second generation version of the Expand Long Template PCR System optimized and designed to consistently amplify PCR products from 5kb up to 25kb from genomic DNA or up to 40kb from lambda DNA templates The supplied buffer can be used for all PCR product sizes In addition a buffer without MgCl2 and separate vials of MgCl2 and DMSO are included if optimization is needed Expand Long Range dNTPack contains a special enzyme mix that consists of thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity This powerful polymerase mixture produces a high yield of PCR product Due to the inherent 3 5 exonuclease activity of the proofreading polymerase Expand Long Range dNTPack copies DNA three times more accurately than Taq DNA Polymerase
    https://www.bioz.com/result/expand long range dntpack/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand long range dntpack - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Mitochondrial DNA sequencing reveals association of variants and haplogroup M33a2′3 with High altitude pulmonary edema susceptibility in Indian male lowlanders
    Article Snippet: .. Long Range PCR was done using Expand Long Range dNTPack (Sigma Aldirch cat no. 4829034001). ..

    Article Title: Molecular evolution at a meiosis gene mediates species differences in the rate and patterning of recombination
    Article Snippet: .. The full-length mei-217/-218 gene, and all of the flanking 5’ and 3’ noncoding regions, was PCR-amplified with Expand Long Range dNTPack PCR System (Sigma-Aldrich Co., Carlsbad, CA) from the genome reference strains, D. mauritiana w ( mau12 w ) and D. melanogaster ( iso-1 ). .. Both clones are anchored in the sequences of the neighboring protein codng genes, CG5004 and RpS5a .

    SYBR Green Assay:

    Article Title: The impact of poly-A microsatellite heterologies in meiotic recombination
    Article Snippet: .. PCRs were set up in a volume of 10 μl containing 0.35 U of Expand Long Range Polymerase (Sigma-Aldrich), 1× Expand Long Range Standard Buffer (Sigma-Aldrich), 0.2 mM Expand Long Range dNTPack dNTPs (Sigma-Aldrich), 0.4 μM allele-specific forward primer and 0.4 μM allele-specific reverse primer (both Eurofins Scientific), 1× SYBR Green I in DMSO (Invitrogen), and 50 ng genomic DNA. .. Details are in the Materials and Methods (PCR conditions for haplotyping) section of the Supplementary Information .

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  • 96
    Millipore expand high fidelity pcr system
    Expand High Fidelity Pcr System, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity pcr system/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr system - by Bioz Stars, 2020-07
    96/100 stars
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