expand long range dntpack  (Millipore)

 
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    Name:
    Expand Long Range dNTPack
    Description:
    The Expand Long Range dNTPack is the second generation version of the Expand Long Template PCR System optimized and designed to consistently amplify PCR products from 5kb up to 25kb from genomic DNA or up to 40kb from lambda DNA templates The supplied buffer can be used for all PCR product sizes In addition a buffer without MgCl2 and separate vials of MgCl2 and DMSO are included if optimization is needed Expand Long Range dNTPack contains a special enzyme mix that consists of thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity This powerful polymerase mixture produces a high yield of PCR product Due to the inherent 3 5 exonuclease activity of the proofreading polymerase Expand Long Range dNTPack copies DNA three times more accurately than Taq DNA Polymerase
    Catalog Number:
    ELONGN-RO
    Price:
    None
    Applications:
    The Expand Long Range dNTPack is optimized to efficiently amplify large genomic DNA fragments from 5kb to 25kb in combination with a threefold higher fidelity than Taq DNA Polymerase. The Expand Long Range dNTPack is ideally suited for: . Genome mapping and sequencing. Contig construction. Characterization of cloned sequences in lambda phages or cosmids. Cloning and analysis of eukaryotic genes. Rapid identification and cloning of complete genes from genomic DNA using cDNA sequence as starting material. Two-step RT-PCRUse the specially designed buffer set, ultrapure PCR Nucleotide Mix, and optimized blend of thermostable DNA polymerases for:. PCR. Large-fragment amplification. RT-PCRFor amplicon sizes of 20 to 35kb choose Expand 20kb PLUS PCR System, dNTPack.
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    Structured Review

    Millipore expand long range dntpack
    Expand Long Range dNTPack
    The Expand Long Range dNTPack is the second generation version of the Expand Long Template PCR System optimized and designed to consistently amplify PCR products from 5kb up to 25kb from genomic DNA or up to 40kb from lambda DNA templates The supplied buffer can be used for all PCR product sizes In addition a buffer without MgCl2 and separate vials of MgCl2 and DMSO are included if optimization is needed Expand Long Range dNTPack contains a special enzyme mix that consists of thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity This powerful polymerase mixture produces a high yield of PCR product Due to the inherent 3 5 exonuclease activity of the proofreading polymerase Expand Long Range dNTPack copies DNA three times more accurately than Taq DNA Polymerase
    https://www.bioz.com/result/expand long range dntpack/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand long range dntpack - by Bioz Stars, 2021-07
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae.
    Article Snippet: T. urticae DNA was extracted from the WT strain using the Gentra Puregene Tissue Kit (QIAgen), according to the manufacturer's instructions and using 100 adult females as starting material. .. The PCR of tetur01g11270 fragments (amplicon 1 and 2) was conducted using the Expand™ Long Range dNTPack (Sigma-Aldrich). .. PCR reaction mixtures were prepared according to the manufacturer's instructions and using the following temperature profile: denaturation for 2 min at 92 °C, followed by five touch-down cycles of denaturation at 92 °C for 10 s, annealing at 60 °C–1 °C/cycle for 15 s and elongation at 68 °C for 1 min. Next, 37 cycles of 92 °C for 10 s, 55 °C for 15 s and 68 °C for 1 min. After a final elongation of 68 °C for 5 min, PCR products were checked by agarose gel electrophoresis, and purified using the EZNA® Cycle Pure Kit (Omega Bio-Tek).

    Article Title: Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae
    Article Snippet: T. urticae DNA was extracted from the WT strain using the Gentra Puregene Tissue Kit (QIAgen), according to the manufacturer’s instructions and using 100 adult females as starting material. .. The PCR of tetur01g11270 fragments (amplicon 1 and 2) was conducted using the Expand™ Long Range dNTPack (Sigma-Aldrich). .. PCR reaction mixtures were prepared according to the manufacturer’s instructions and using the following temperature profile: denaturation for 2 min at 92°C, followed by five touch-down cycles of denaturation at 92°C for 10 s, annealing at 60°C −1°C/cycle for 15 s and elongation at 68°C for 1 min. Next, 37 cycles of 92°C for 10 s, 55°C for 15 s and 68°C for 1 min. After a final elongation of 68°C for 5 min, PCR products were checked by agarose gel electrophoresis, and purified using the EZNA® Cycle Pure Kit (Omega Bio-Tek).

    Article Title: Molecular evolution at a meiosis gene mediates species differences in the rate and patterning of recombination
    Article Snippet: To create transgenic flies we used the Φ C31 integrase-mediated transgenesis system, which allows for site-specific integration [ ]. .. The full-length mei-217/-218 gene, and all of the flanking 5’ and 3’ noncoding regions, was PCR-amplified with Expand Long Range dNTPack PCR System (Sigma-Aldrich Co., Carlsbad, CA) from the genome reference strains, D. mauritiana w ( mau12 w ) and D. melanogaster ( iso-1 ). .. Both clones are anchored in the sequences of the neighboring protein codng genes, CG5004 and RpS5a .

    Article Title: Mitochondrial DNA sequencing reveals association of variants and haplogroup M33a2′3 with High altitude pulmonary edema susceptibility in Indian male lowlanders
    Article Snippet: .. Long Range PCR was done using Expand Long Range dNTPack (Sigma Aldirch cat no. 4829034001). ..

    Amplification:

    Article Title: Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae.
    Article Snippet: T. urticae DNA was extracted from the WT strain using the Gentra Puregene Tissue Kit (QIAgen), according to the manufacturer's instructions and using 100 adult females as starting material. .. The PCR of tetur01g11270 fragments (amplicon 1 and 2) was conducted using the Expand™ Long Range dNTPack (Sigma-Aldrich). .. PCR reaction mixtures were prepared according to the manufacturer's instructions and using the following temperature profile: denaturation for 2 min at 92 °C, followed by five touch-down cycles of denaturation at 92 °C for 10 s, annealing at 60 °C–1 °C/cycle for 15 s and elongation at 68 °C for 1 min. Next, 37 cycles of 92 °C for 10 s, 55 °C for 15 s and 68 °C for 1 min. After a final elongation of 68 °C for 5 min, PCR products were checked by agarose gel electrophoresis, and purified using the EZNA® Cycle Pure Kit (Omega Bio-Tek).

    Article Title: Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae
    Article Snippet: T. urticae DNA was extracted from the WT strain using the Gentra Puregene Tissue Kit (QIAgen), according to the manufacturer’s instructions and using 100 adult females as starting material. .. The PCR of tetur01g11270 fragments (amplicon 1 and 2) was conducted using the Expand™ Long Range dNTPack (Sigma-Aldrich). .. PCR reaction mixtures were prepared according to the manufacturer’s instructions and using the following temperature profile: denaturation for 2 min at 92°C, followed by five touch-down cycles of denaturation at 92°C for 10 s, annealing at 60°C −1°C/cycle for 15 s and elongation at 68°C for 1 min. Next, 37 cycles of 92°C for 10 s, 55°C for 15 s and 68°C for 1 min. After a final elongation of 68°C for 5 min, PCR products were checked by agarose gel electrophoresis, and purified using the EZNA® Cycle Pure Kit (Omega Bio-Tek).

    Article Title: Next-Generation Sequencing-Assisted DNA-Based Digital PCR for a Personalized Approach to the Detection and Quantification of Residual Disease in Chronic Myeloid Leukemia Patients.
    Article Snippet: Total reaction mixes, volume per inlet, thermocycling conditions, and data analysis software were as described for dPCR of DNA (see dPCR Quantification). .. Threshold and amplification range across the panels of the same assay were set according to the parameters defined during the validation stage. .. 184 qPCR of BCR-ABL1 DNA Hydrolysis probe assays were as previously detailed for dPCR (see dPCR Quantification).

    Article Title: RasGRP2 inhibits glyceraldehyde-derived toxic advanced glycation end-products from inducing permeability in vascular endothelial cells
    Article Snippet: .. Briefly, the DNA fragment of rasgrp2 was amplified from human placenta cDNA (K1420-1; Clontech) using the Expand Long Range dNTPack (4829042001; Sigma-Aldrich), pEB Multi-Hyg (050-08121; FUJIFILM Wako Pure Chemical Corporation) was used as a vector, and ViaFect Transfection Reagent (E4981; Promega) was used as the transfection reagent. .. Cells were seeded in a 24-well plate, grown overnight, transfected, and purified to prepare TERT HUVEC R and mock cell lines.

    Plasmid Preparation:

    Article Title: RasGRP2 inhibits glyceraldehyde-derived toxic advanced glycation end-products from inducing permeability in vascular endothelial cells
    Article Snippet: .. Briefly, the DNA fragment of rasgrp2 was amplified from human placenta cDNA (K1420-1; Clontech) using the Expand Long Range dNTPack (4829042001; Sigma-Aldrich), pEB Multi-Hyg (050-08121; FUJIFILM Wako Pure Chemical Corporation) was used as a vector, and ViaFect Transfection Reagent (E4981; Promega) was used as the transfection reagent. .. Cells were seeded in a 24-well plate, grown overnight, transfected, and purified to prepare TERT HUVEC R and mock cell lines.

    Transfection:

    Article Title: RasGRP2 inhibits glyceraldehyde-derived toxic advanced glycation end-products from inducing permeability in vascular endothelial cells
    Article Snippet: .. Briefly, the DNA fragment of rasgrp2 was amplified from human placenta cDNA (K1420-1; Clontech) using the Expand Long Range dNTPack (4829042001; Sigma-Aldrich), pEB Multi-Hyg (050-08121; FUJIFILM Wako Pure Chemical Corporation) was used as a vector, and ViaFect Transfection Reagent (E4981; Promega) was used as the transfection reagent. .. Cells were seeded in a 24-well plate, grown overnight, transfected, and purified to prepare TERT HUVEC R and mock cell lines.

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  • About
  • News
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  • Contact
  • Bioz Stars
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  • 93
    Millipore expand long range dntpack
    Expand Long Range Dntpack, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand long range dntpack/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand long range dntpack - by Bioz Stars, 2021-07
    93/100 stars
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