expand high fidelity pcr  (GE Healthcare)

 
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    Expander ring
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    Structured Review

    GE Healthcare expand high fidelity pcr

    https://www.bioz.com/result/expand high fidelity pcr/product/GE Healthcare
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr - by Bioz Stars, 2021-07
    88/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Probing Direct Interactions between CodY and the oppD Promoter of Lactococcus lactis
    Article Snippet: DNase I footprinting was performed essentially according to the description supplied with the Sure Track footprinting kit (Amersham Pharmacia Biotech). .. The DNA fragments were prepared by PCR using Expand DNA polymerase (Amersham Pharmacia Biotech) and oligonucleotides opp1 and opp3, one of which was first end labeled with T4 polynucleotide kinase (Amersham Pharmacia Biotech) and [γ-32 P]ATP as described by the manufacturer. .. Binding reactions were identical to those used in EMSAs, in a total volume of 40 μl and in the presence of approximately 150,000 cpm of the DNA probe.

    Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
    Article Snippet: Midiprep (HiSpeed Plasmid Midi Kit) and DNA extraction kits (QIAamp DNA extraction kit) were purchased from QIAGEN. .. The plasmid, pILL2000, was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 772-1 (5′-GAUGAUGAUGGTACCAAGGATTTTAACTTCATAAGTC-3′ in which the underlined sequence corresponds to a Kpn I site) and 772-2 (5′-AUCAUCAUCGGATCCAACACGCAGCGATTGATCGTCTCTAAC-3′ the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase; Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

    Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
    Article Snippet: The final PCR product was obtained by using a mixture of these three fragments as a template and oligonucleotides 954F and 954Rev. .. For the reverse orientation, the pILL570-rdxA plasmid was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 954–2KpnI (5′-cgg ggtacc tacatgcaaaatctctatccg-3′, in which the underlined sequence corresponds to a Kpn I site) and 954–1Bam HI (5′-cgc ggatcc gtgtggtaacaactcgctggg-3′, in which the underlined sequence corresponds to a Bam HI site). .. The amiA gene was amplified using the following primers: 772-compl-1Bis (5′-cgg ggatcc gagggttaatttgtagtgcttgtgaggttagggg-3′, in which the underlined sequence corresponds to a Bam HI site) and 772-compl-2Bis (5′-cg ggtacc ctaatcattcttgctgaaaaactatcaatgcc-3′, in which the underlined sequence corresponds to a Kpn I site).

    Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
    Article Snippet: For complementation, the promorterless WT amiA gene was introduced in the rdxA gene carried by plasmid pILL570. .. The plasmid was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 954-2KpnI (5′-CGG GGTACC TACATGCAAAATCTCTATCCG-3′ in which the underlined sequence corresponds to a Kpn I site) and 954-1BamHI (5′- CGCGGATCC GTGTGGTAACAACTCGCTGGG-3′ the underlined sequence corresponds to a Bam HI site). .. The amiA gene was amplified using the following primers: 772-compl-1Bis (5′-GG GGATCC GAGGGTTAATTTGTAGTGCTTGTGAGGTTAGGGG-3′ in which the underlined sequence corresponds to a Bam HI site) and 772-compl-2Bis (5′-CG GGTACC CTAATCATTCTTGCTGAAAAACTATCAATGCC-3′ the underlined sequence corresponds to a Kpn I site).

    Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
    Article Snippet: Midiprep (HiSpeed Plasmid Midi Kit) and DNA extraction kits (QIAamp DNA extraction kit) were purchased from Qiagen ( http://www.qiagen.com ). .. The plasmid pILL2000 was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham, http://www.amershambiosciences.com ) with oligonucleotides 772–1 (5′-gaugaugau ggtacc aaggattttaacttcataagtc-3′, in which the underlined sequence corresponds to a Kpn I site) and 772–2 (5′-aucaucauc ggatcc aacacgcagcgattgatcgtctctaac-3′, in which the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase, Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

    Article Title: Expression profiling of Drosophila imaginal discs
    Article Snippet: The amplification products were purified with 96-well format Qiagen PCR purification columns. .. All PCR products were analyzed on agarose gels, and reactions with no detectable product, multiple bands or bands of unexpected size were repeated using Herculase (Amersham) or Expand polymerase (Roche). .. One hundred and seventy-five cDNAs from our lab collection were amplified and added to the set, resulting in a total of 6,024 cDNAs.

    Labeling:

    Article Title: Probing Direct Interactions between CodY and the oppD Promoter of Lactococcus lactis
    Article Snippet: DNase I footprinting was performed essentially according to the description supplied with the Sure Track footprinting kit (Amersham Pharmacia Biotech). .. The DNA fragments were prepared by PCR using Expand DNA polymerase (Amersham Pharmacia Biotech) and oligonucleotides opp1 and opp3, one of which was first end labeled with T4 polynucleotide kinase (Amersham Pharmacia Biotech) and [γ-32 P]ATP as described by the manufacturer. .. Binding reactions were identical to those used in EMSAs, in a total volume of 40 μl and in the presence of approximately 150,000 cpm of the DNA probe.

    Plasmid Preparation:

    Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
    Article Snippet: Midiprep (HiSpeed Plasmid Midi Kit) and DNA extraction kits (QIAamp DNA extraction kit) were purchased from QIAGEN. .. The plasmid, pILL2000, was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 772-1 (5′-GAUGAUGAUGGTACCAAGGATTTTAACTTCATAAGTC-3′ in which the underlined sequence corresponds to a Kpn I site) and 772-2 (5′-AUCAUCAUCGGATCCAACACGCAGCGATTGATCGTCTCTAAC-3′ the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase; Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

    Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
    Article Snippet: The final PCR product was obtained by using a mixture of these three fragments as a template and oligonucleotides 954F and 954Rev. .. For the reverse orientation, the pILL570-rdxA plasmid was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 954–2KpnI (5′-cgg ggtacc tacatgcaaaatctctatccg-3′, in which the underlined sequence corresponds to a Kpn I site) and 954–1Bam HI (5′-cgc ggatcc gtgtggtaacaactcgctggg-3′, in which the underlined sequence corresponds to a Bam HI site). .. The amiA gene was amplified using the following primers: 772-compl-1Bis (5′-cgg ggatcc gagggttaatttgtagtgcttgtgaggttagggg-3′, in which the underlined sequence corresponds to a Bam HI site) and 772-compl-2Bis (5′-cg ggtacc ctaatcattcttgctgaaaaactatcaatgcc-3′, in which the underlined sequence corresponds to a Kpn I site).

    Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
    Article Snippet: For complementation, the promorterless WT amiA gene was introduced in the rdxA gene carried by plasmid pILL570. .. The plasmid was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 954-2KpnI (5′-CGG GGTACC TACATGCAAAATCTCTATCCG-3′ in which the underlined sequence corresponds to a Kpn I site) and 954-1BamHI (5′- CGCGGATCC GTGTGGTAACAACTCGCTGGG-3′ the underlined sequence corresponds to a Bam HI site). .. The amiA gene was amplified using the following primers: 772-compl-1Bis (5′-GG GGATCC GAGGGTTAATTTGTAGTGCTTGTGAGGTTAGGGG-3′ in which the underlined sequence corresponds to a Bam HI site) and 772-compl-2Bis (5′-CG GGTACC CTAATCATTCTTGCTGAAAAACTATCAATGCC-3′ the underlined sequence corresponds to a Kpn I site).

    Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
    Article Snippet: Midiprep (HiSpeed Plasmid Midi Kit) and DNA extraction kits (QIAamp DNA extraction kit) were purchased from Qiagen ( http://www.qiagen.com ). .. The plasmid pILL2000 was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham, http://www.amershambiosciences.com ) with oligonucleotides 772–1 (5′-gaugaugau ggtacc aaggattttaacttcataagtc-3′, in which the underlined sequence corresponds to a Kpn I site) and 772–2 (5′-aucaucauc ggatcc aacacgcagcgattgatcgtctctaac-3′, in which the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase, Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

    Construct:

    Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
    Article Snippet: Midiprep (HiSpeed Plasmid Midi Kit) and DNA extraction kits (QIAamp DNA extraction kit) were purchased from QIAGEN. .. The plasmid, pILL2000, was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 772-1 (5′-GAUGAUGAUGGTACCAAGGATTTTAACTTCATAAGTC-3′ in which the underlined sequence corresponds to a Kpn I site) and 772-2 (5′-AUCAUCAUCGGATCCAACACGCAGCGATTGATCGTCTCTAAC-3′ the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase; Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

    Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
    Article Snippet: Midiprep (HiSpeed Plasmid Midi Kit) and DNA extraction kits (QIAamp DNA extraction kit) were purchased from Qiagen ( http://www.qiagen.com ). .. The plasmid pILL2000 was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham, http://www.amershambiosciences.com ) with oligonucleotides 772–1 (5′-gaugaugau ggtacc aaggattttaacttcataagtc-3′, in which the underlined sequence corresponds to a Kpn I site) and 772–2 (5′-aucaucauc ggatcc aacacgcagcgattgatcgtctctaac-3′, in which the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase, Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

    Mutagenesis:

    Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
    Article Snippet: Midiprep (HiSpeed Plasmid Midi Kit) and DNA extraction kits (QIAamp DNA extraction kit) were purchased from QIAGEN. .. The plasmid, pILL2000, was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 772-1 (5′-GAUGAUGAUGGTACCAAGGATTTTAACTTCATAAGTC-3′ in which the underlined sequence corresponds to a Kpn I site) and 772-2 (5′-AUCAUCAUCGGATCCAACACGCAGCGATTGATCGTCTCTAAC-3′ the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase; Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

    Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
    Article Snippet: Midiprep (HiSpeed Plasmid Midi Kit) and DNA extraction kits (QIAamp DNA extraction kit) were purchased from Qiagen ( http://www.qiagen.com ). .. The plasmid pILL2000 was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham, http://www.amershambiosciences.com ) with oligonucleotides 772–1 (5′-gaugaugau ggtacc aaggattttaacttcataagtc-3′, in which the underlined sequence corresponds to a Kpn I site) and 772–2 (5′-aucaucauc ggatcc aacacgcagcgattgatcgtctctaac-3′, in which the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase, Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

    Sequencing:

    Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
    Article Snippet: Midiprep (HiSpeed Plasmid Midi Kit) and DNA extraction kits (QIAamp DNA extraction kit) were purchased from QIAGEN. .. The plasmid, pILL2000, was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 772-1 (5′-GAUGAUGAUGGTACCAAGGATTTTAACTTCATAAGTC-3′ in which the underlined sequence corresponds to a Kpn I site) and 772-2 (5′-AUCAUCAUCGGATCCAACACGCAGCGATTGATCGTCTCTAAC-3′ the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase; Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

    Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
    Article Snippet: The final PCR product was obtained by using a mixture of these three fragments as a template and oligonucleotides 954F and 954Rev. .. For the reverse orientation, the pILL570-rdxA plasmid was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 954–2KpnI (5′-cgg ggtacc tacatgcaaaatctctatccg-3′, in which the underlined sequence corresponds to a Kpn I site) and 954–1Bam HI (5′-cgc ggatcc gtgtggtaacaactcgctggg-3′, in which the underlined sequence corresponds to a Bam HI site). .. The amiA gene was amplified using the following primers: 772-compl-1Bis (5′-cgg ggatcc gagggttaatttgtagtgcttgtgaggttagggg-3′, in which the underlined sequence corresponds to a Bam HI site) and 772-compl-2Bis (5′-cg ggtacc ctaatcattcttgctgaaaaactatcaatgcc-3′, in which the underlined sequence corresponds to a Kpn I site).

    Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
    Article Snippet: For complementation, the promorterless WT amiA gene was introduced in the rdxA gene carried by plasmid pILL570. .. The plasmid was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 954-2KpnI (5′-CGG GGTACC TACATGCAAAATCTCTATCCG-3′ in which the underlined sequence corresponds to a Kpn I site) and 954-1BamHI (5′- CGCGGATCC GTGTGGTAACAACTCGCTGGG-3′ the underlined sequence corresponds to a Bam HI site). .. The amiA gene was amplified using the following primers: 772-compl-1Bis (5′-GG GGATCC GAGGGTTAATTTGTAGTGCTTGTGAGGTTAGGGG-3′ in which the underlined sequence corresponds to a Bam HI site) and 772-compl-2Bis (5′-CG GGTACC CTAATCATTCTTGCTGAAAAACTATCAATGCC-3′ the underlined sequence corresponds to a Kpn I site).

    Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
    Article Snippet: Midiprep (HiSpeed Plasmid Midi Kit) and DNA extraction kits (QIAamp DNA extraction kit) were purchased from Qiagen ( http://www.qiagen.com ). .. The plasmid pILL2000 was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham, http://www.amershambiosciences.com ) with oligonucleotides 772–1 (5′-gaugaugau ggtacc aaggattttaacttcataagtc-3′, in which the underlined sequence corresponds to a Kpn I site) and 772–2 (5′-aucaucauc ggatcc aacacgcagcgattgatcgtctctaac-3′, in which the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase, Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

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  • 88
    GE Healthcare expand high fidelity pcr
    Expand High Fidelity Pcr, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity pcr/product/GE Healthcare
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr - by Bioz Stars, 2021-07
    88/100 stars
      Buy from Supplier

    94
    GE Healthcare expand high fidelity pcr system dna polymerase
    Expand High Fidelity Pcr System Dna Polymerase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity pcr system dna polymerase/product/GE Healthcare
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr system dna polymerase - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

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