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GE Healthcare expand high fidelity pcr
Expand High Fidelity Pcr, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expand high fidelity pcr/product/GE Healthcare
Average 88 stars, based on 4 article reviews
Price from $9.99 to $1999.99
expand high fidelity pcr - by Bioz Stars, 2020-07
88/100 stars

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Polymerase Chain Reaction:

Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
Article Snippet: .. For the reverse orientation, the pILL570-rdxA plasmid was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 954–2KpnI (5′-cgg ggtacc tacatgcaaaatctctatccg-3′, in which the underlined sequence corresponds to a Kpn I site) and 954–1Bam HI (5′-cgc ggatcc gtgtggtaacaactcgctggg-3′, in which the underlined sequence corresponds to a Bam HI site). .. The amiA gene was amplified using the following primers: 772-compl-1Bis (5′-cgg ggatcc gagggttaatttgtagtgcttgtgaggttagggg-3′, in which the underlined sequence corresponds to a Bam HI site) and 772-compl-2Bis (5′-cg ggtacc ctaatcattcttgctgaaaaactatcaatgcc-3′, in which the underlined sequence corresponds to a Kpn I site).

Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
Article Snippet: .. The plasmid pILL2000 was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham, http://www.amershambiosciences.com ) with oligonucleotides 772–1 (5′-gaugaugau ggtacc aaggattttaacttcataagtc-3′, in which the underlined sequence corresponds to a Kpn I site) and 772–2 (5′-aucaucauc ggatcc aacacgcagcgattgatcgtctctaac-3′, in which the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase, Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
Article Snippet: .. The plasmid, pILL2000, was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 772-1 (5′-GAUGAUGAUGGTACCAAGGATTTTAACTTCATAAGTC-3′ in which the underlined sequence corresponds to a Kpn I site) and 772-2 (5′-AUCAUCAUCGGATCCAACACGCAGCGATTGATCGTCTCTAAC-3′ the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase; Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
Article Snippet: .. The plasmid was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 954-2KpnI (5′-CGG GGTACC TACATGCAAAATCTCTATCCG-3′ in which the underlined sequence corresponds to a Kpn I site) and 954-1BamHI (5′- CGCGGATCC GTGTGGTAACAACTCGCTGGG-3′ the underlined sequence corresponds to a Bam HI site). .. The amiA gene was amplified using the following primers: 772-compl-1Bis (5′-GG GGATCC GAGGGTTAATTTGTAGTGCTTGTGAGGTTAGGGG-3′ in which the underlined sequence corresponds to a Bam HI site) and 772-compl-2Bis (5′-CG GGTACC CTAATCATTCTTGCTGAAAAACTATCAATGCC-3′ the underlined sequence corresponds to a Kpn I site).

Sequencing:

Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
Article Snippet: .. For the reverse orientation, the pILL570-rdxA plasmid was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 954–2KpnI (5′-cgg ggtacc tacatgcaaaatctctatccg-3′, in which the underlined sequence corresponds to a Kpn I site) and 954–1Bam HI (5′-cgc ggatcc gtgtggtaacaactcgctggg-3′, in which the underlined sequence corresponds to a Bam HI site). .. The amiA gene was amplified using the following primers: 772-compl-1Bis (5′-cgg ggatcc gagggttaatttgtagtgcttgtgaggttagggg-3′, in which the underlined sequence corresponds to a Bam HI site) and 772-compl-2Bis (5′-cg ggtacc ctaatcattcttgctgaaaaactatcaatgcc-3′, in which the underlined sequence corresponds to a Kpn I site).

Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
Article Snippet: .. The plasmid pILL2000 was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham, http://www.amershambiosciences.com ) with oligonucleotides 772–1 (5′-gaugaugau ggtacc aaggattttaacttcataagtc-3′, in which the underlined sequence corresponds to a Kpn I site) and 772–2 (5′-aucaucauc ggatcc aacacgcagcgattgatcgtctctaac-3′, in which the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase, Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
Article Snippet: .. The plasmid, pILL2000, was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 772-1 (5′-GAUGAUGAUGGTACCAAGGATTTTAACTTCATAAGTC-3′ in which the underlined sequence corresponds to a Kpn I site) and 772-2 (5′-AUCAUCAUCGGATCCAACACGCAGCGATTGATCGTCTCTAAC-3′ the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase; Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
Article Snippet: .. The plasmid was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 954-2KpnI (5′-CGG GGTACC TACATGCAAAATCTCTATCCG-3′ in which the underlined sequence corresponds to a Kpn I site) and 954-1BamHI (5′- CGCGGATCC GTGTGGTAACAACTCGCTGGG-3′ the underlined sequence corresponds to a Bam HI site). .. The amiA gene was amplified using the following primers: 772-compl-1Bis (5′-GG GGATCC GAGGGTTAATTTGTAGTGCTTGTGAGGTTAGGGG-3′ in which the underlined sequence corresponds to a Bam HI site) and 772-compl-2Bis (5′-CG GGTACC CTAATCATTCTTGCTGAAAAACTATCAATGCC-3′ the underlined sequence corresponds to a Kpn I site).

Mutagenesis:

Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
Article Snippet: .. The plasmid pILL2000 was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham, http://www.amershambiosciences.com ) with oligonucleotides 772–1 (5′-gaugaugau ggtacc aaggattttaacttcataagtc-3′, in which the underlined sequence corresponds to a Kpn I site) and 772–2 (5′-aucaucauc ggatcc aacacgcagcgattgatcgtctctaac-3′, in which the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase, Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
Article Snippet: .. The plasmid, pILL2000, was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 772-1 (5′-GAUGAUGAUGGTACCAAGGATTTTAACTTCATAAGTC-3′ in which the underlined sequence corresponds to a Kpn I site) and 772-2 (5′-AUCAUCAUCGGATCCAACACGCAGCGATTGATCGTCTCTAAC-3′ the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase; Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

Plasmid Preparation:

Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
Article Snippet: .. For the reverse orientation, the pILL570-rdxA plasmid was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 954–2KpnI (5′-cgg ggtacc tacatgcaaaatctctatccg-3′, in which the underlined sequence corresponds to a Kpn I site) and 954–1Bam HI (5′-cgc ggatcc gtgtggtaacaactcgctggg-3′, in which the underlined sequence corresponds to a Bam HI site). .. The amiA gene was amplified using the following primers: 772-compl-1Bis (5′-cgg ggatcc gagggttaatttgtagtgcttgtgaggttagggg-3′, in which the underlined sequence corresponds to a Bam HI site) and 772-compl-2Bis (5′-cg ggtacc ctaatcattcttgctgaaaaactatcaatgcc-3′, in which the underlined sequence corresponds to a Kpn I site).

Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
Article Snippet: .. The plasmid pILL2000 was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham, http://www.amershambiosciences.com ) with oligonucleotides 772–1 (5′-gaugaugau ggtacc aaggattttaacttcataagtc-3′, in which the underlined sequence corresponds to a Kpn I site) and 772–2 (5′-aucaucauc ggatcc aacacgcagcgattgatcgtctctaac-3′, in which the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase, Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
Article Snippet: .. The plasmid, pILL2000, was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 772-1 (5′-GAUGAUGAUGGTACCAAGGATTTTAACTTCATAAGTC-3′ in which the underlined sequence corresponds to a Kpn I site) and 772-2 (5′-AUCAUCAUCGGATCCAACACGCAGCGATTGATCGTCTCTAAC-3′ the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase; Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
Article Snippet: .. The plasmid was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 954-2KpnI (5′-CGG GGTACC TACATGCAAAATCTCTATCCG-3′ in which the underlined sequence corresponds to a Kpn I site) and 954-1BamHI (5′- CGCGGATCC GTGTGGTAACAACTCGCTGGG-3′ the underlined sequence corresponds to a Bam HI site). .. The amiA gene was amplified using the following primers: 772-compl-1Bis (5′-GG GGATCC GAGGGTTAATTTGTAGTGCTTGTGAGGTTAGGGG-3′ in which the underlined sequence corresponds to a Bam HI site) and 772-compl-2Bis (5′-CG GGTACC CTAATCATTCTTGCTGAAAAACTATCAATGCC-3′ the underlined sequence corresponds to a Kpn I site).

Construct:

Article Title: Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
Article Snippet: .. The plasmid pILL2000 was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham, http://www.amershambiosciences.com ) with oligonucleotides 772–1 (5′-gaugaugau ggtacc aaggattttaacttcataagtc-3′, in which the underlined sequence corresponds to a Kpn I site) and 772–2 (5′-aucaucauc ggatcc aacacgcagcgattgatcgtctctaac-3′, in which the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase, Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

Article Title: Role of the N-Acetylmuramoyl-l-Alanyl Amidase, AmiA, of Helicobacter pylori in Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence
Article Snippet: .. The plasmid, pILL2000, was used to construct the amiA mutant. pILL570 carrying ORF hp0772 (amiA gene) was used as the template for an Expand High Fidelity PCR (Amersham) with oligonucleotides 772-1 (5′-GAUGAUGAUGGTACCAAGGATTTTAACTTCATAAGTC-3′ in which the underlined sequence corresponds to a Kpn I site) and 772-2 (5′-AUCAUCAUCGGATCCAACACGCAGCGATTGATCGTCTCTAAC-3′ the underlined sequence corresponds to a Bam HI site). .. PCR products were digested with Bam HI (Amersham) and Kpn I (Amersham) and ligated (T4 DNA ligase; Amersham) with the aphA-3 nonpolar cassette digested with the same endonucleases.

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    GE Healthcare illustra blood genomicprep kit
    Illustra Blood Genomicprep Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illustra blood genomicprep kit/product/GE Healthcare
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    illustra blood genomicprep kit - by Bioz Stars, 2020-07
    85/100 stars
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