expand high fidelity pcr system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher expand high fidelity pcr system
    Nucleotide sequence of the <t>PCR-generated</t> fragment containing the wild-type <t>nfsA</t> gene from E. coli ). Mutations for the 20 mutants characterized in this study are indicated. Altered bases and amino acids are underlined in the wild-type sequence, with substituted bases indicated above the sequence. IS elements are shown as boxes above the sequence, with arrows indicating the insertion point. Deletions and frameshifts are indicated below the sequence. PCR primers used for amplification of the gene are shown as horizontal arrows, and the lowercase letters indicate synthetic restriction endonuclease sites. S.D., Shine-Dalgarno sequence.
    Expand High Fidelity Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity pcr system/product/Thermo Fisher
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr system - by Bioz Stars, 2020-07
    92/100 stars

    Images

    1) Product Images from "Oxygen-Insensitive Nitroreductases: Analysis of the Roles of nfsA and nfsB in Development of Resistance to 5-Nitrofuran Derivatives in Escherichia coli"

    Article Title: Oxygen-Insensitive Nitroreductases: Analysis of the Roles of nfsA and nfsB in Development of Resistance to 5-Nitrofuran Derivatives in Escherichia coli

    Journal: Journal of Bacteriology

    doi:

    Nucleotide sequence of the PCR-generated fragment containing the wild-type nfsA gene from E. coli ). Mutations for the 20 mutants characterized in this study are indicated. Altered bases and amino acids are underlined in the wild-type sequence, with substituted bases indicated above the sequence. IS elements are shown as boxes above the sequence, with arrows indicating the insertion point. Deletions and frameshifts are indicated below the sequence. PCR primers used for amplification of the gene are shown as horizontal arrows, and the lowercase letters indicate synthetic restriction endonuclease sites. S.D., Shine-Dalgarno sequence.
    Figure Legend Snippet: Nucleotide sequence of the PCR-generated fragment containing the wild-type nfsA gene from E. coli ). Mutations for the 20 mutants characterized in this study are indicated. Altered bases and amino acids are underlined in the wild-type sequence, with substituted bases indicated above the sequence. IS elements are shown as boxes above the sequence, with arrows indicating the insertion point. Deletions and frameshifts are indicated below the sequence. PCR primers used for amplification of the gene are shown as horizontal arrows, and the lowercase letters indicate synthetic restriction endonuclease sites. S.D., Shine-Dalgarno sequence.

    Techniques Used: Sequencing, Polymerase Chain Reaction, Generated, Amplification

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    Clone Assay:

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    Article Snippet: .. The PCR reactions for cloning experiments were performed using the High Fidelity Platinum® Taq polymerase or the Expand High Fidelity PCR System according to the manufacturer's instructions (Invitrogen). .. The amplified fragments were purified, double-digested by MfeI/BamHI for cloning at the EcoRI/BamHI sites of pASK-IBA33plus to obtain ClbP-RGS6His (ClbP + C-terminal in-frame RGS6His tag), or double-digested by and cloned at the NdeI/NotI sites of pET9am to obtain pClbPpep (ClbP catalytic domain).

    Amplification:

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    Article Title: A hypomorphic mouse model of dystrophic epidermolysis bullosa reveals mechanisms of disease and response to fibroblast therapy
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    Isolation:

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    Mouse Assay:

    Article Title: A hypomorphic mouse model of dystrophic epidermolysis bullosa reveals mechanisms of disease and response to fibroblast therapy
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    Produced:

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    Polymerase Chain Reaction:

    Article Title: Axonal and Schwann Cell BACE1 Is Equally Required for Remyelination of Peripheral Nerves
    Article Snippet: .. DNA templates for BACE1, type I Nrg1, and type III Nrg1 containing the sequences of T7 and T3 promoters were produced by PCR using the Expand High Fidelity PCR System (Life Technologies). ..

    Article Title: Oxygen-Insensitive Nitroreductases: Analysis of the Roles of nfsA and nfsB in Development of Resistance to 5-Nitrofuran Derivatives in Escherichia coli
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    Article Title: The Arabidopsis BEL1-LIKE HOMEODOMAIN Proteins SAW1 and SAW2 Act Redundantly to Regulate KNOX Expression Spatially in Leaf Margins
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    Article Title: Appendage expression driven by the Hoxd Global Control Region is an ancient gnathostome feature
    Article Snippet: .. Genomic DNA fragments ranging from 2.5 to 4.3 Kbp, corresponding to mouse, chicken, zebrafish, and skate CsB were isolated using the Expand High Fidelity PCR System (Invitrogen). .. The DNA oligonucleotide sequence can be found in .

    Article Title: A hypomorphic mouse model of dystrophic epidermolysis bullosa reveals mechanisms of disease and response to fibroblast therapy
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    Incubation:

    Article Title: Oxygen-Insensitive Nitroreductases: Analysis of the Roles of nfsA and nfsB in Development of Resistance to 5-Nitrofuran Derivatives in Escherichia coli
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    Plasmid Preparation:

    Article Title: The POLARIS Gene of Arabidopsis Encodes a Predicted Peptide Required for Correct Root Growth and Leaf Vascular Patterning
    Article Snippet: .. Promoter fragments were amplified using the Expand High Fidelity PCR system and ligated into T-tailed pBluescript SK− or pCR2.1-TOPO vector (Invitrogen), then transferred to pΔGUS ( ). .. The promoter–GUS–nopaline synthase terminator cassette was transferred as a HindIII-EcoRI fragment into the binary vector pCIRCE (a gift from M. Bevan, John Innes Centre, Norwich, UK).

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    BAC Assay:

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    Sequencing:

    Article Title: A hypomorphic mouse model of dystrophic epidermolysis bullosa reveals mechanisms of disease and response to fibroblast therapy
    Article Snippet: .. To generate the Col7a1flNeo targeting vector, DNA fragments for homologous recombination and a region containing exon 2 were amplified from tail DNA of 129Sv/CRL mice (accession no. , sequence of a BAC clone containing the genomic region of murine Col7a1 ; sequences 164,009–169,109, 157,233–163,520, and 163,323–164,266) using the Expand High Fidelity PCR system (Invitrogen). .. The BamHI/EcoRI fragment of sequence 164,009–169,109 and a loxP site were introduced upstream of Col7a1 exon 2.

    Homologous Recombination:

    Article Title: A hypomorphic mouse model of dystrophic epidermolysis bullosa reveals mechanisms of disease and response to fibroblast therapy
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    Thermo Fisher rt pcr
    Rt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 17152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt pcr/product/Thermo Fisher
    Average 99 stars, based on 17152 article reviews
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