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Boehringer Mannheim expand high fidelity pcr kit
Expand High Fidelity Pcr Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expand high fidelity pcr kit/product/Boehringer Mannheim
Average 90 stars, based on 13 article reviews
Price from $9.99 to $1999.99
expand high fidelity pcr kit - by Bioz Stars, 2020-07
90/100 stars

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Polymerase Chain Reaction:

Article Title: Discrimination of Subtype B and Non-Subtype B Strains of Human Immunodeficiency Virus Type 1 by Serotyping: Correlation with Genotyping
Article Snippet: .. After stopping the reaction by putting it on ice, the cDNA was immediately amplified by PCR with an Expand High Fidelity PCR kit (catalog no. 1732650; Boehringer Mannheim). ..

Article Title: Full length HIV-1 Gag determines protease inhibitor susceptibility within in vitro assays
Article Snippet: .. Both PCR steps were carried out using Expand® High Fidelity PCR kit (Boehringer Manheim). .. The subtype A gag-protease sequences were cloned into the vector backbone, and representative clones (12.2 and 32G) chosen that had the same pol sequence as determined from plasma virus population sequencing.

Article Title: Recombination activities of HsDmc1 protein, the meiotic human homolog of RecA protein
Article Snippet: .. ATP and adenosine 5′-[γ-thio]triphosphate (ATPγS) were purchased from Sigma; T4 polynucleotide kinase, T4 DNA ligase and restriction enzymes were purchased from New England Biolabs (Beverly, MA); DNase I, DTT, BSA, and an Expand High Fidelity PCR kit were purchased from Boehringer Mannheim. .. Vector pQE-30 was obtained from Qiagen (Chatsworth, CA).

Article Title: Recombination activities of HsDmc1 protein, the meiotic human homolog of RecA protein
Article Snippet: .. The PCR reaction was carried out by using an Expand High Fidelity PCR kit (Boehringer Mannheim). ..

Article Title: A Novel Phenotypic Drug Susceptibility Assay for Human Immunodeficiency Virus Type 1
Article Snippet: .. Reverse transcription was performed with Superscript II (Gibco/BRL, Gaithersburg, Md.) with an antisense internal primer, and PR and RT sequences were amplified with the Expand High Fidelity PCR kit (Boehringer Mannheim, Indianapolis, Ind.) with a forward primer containing an Apa I site and a reverse primer containing a Pin AI site ( ). .. The 1.5-kb amplification product spans the p7-p1-p6 protease cleavage sites in the gag polyprotein, the entire PR coding region, and the RT coding region from amino acids 1 to 313.

Article Title: trans-Complementation of Flavivirus RNA Polymerase Gene NS5 by Using Kunjin Virus Replicon-Expressing BHK Cells
Article Snippet: .. PCR amplification after RT of a 6,895-bp DNA fragment was performed with an Expand High Fidelity PCR kit (Boehringer Mannheim) and with a 1/25 to 1/10 volume of RT product as follows. .. The PCR mixture (50 μl) containing all necessary components except the enzyme mixture (3 parts Taq polymerase and 1 part Pwo polymerase) was preheated at 95°C for 5 min and then the enzyme mixture was added and the following cycles were performed: 10 cycles of 95°C for 15 s and 72°C for 6 min, followed by 6 cycles of 95°C for 15 s and 72°C for 6 min, with an automatic increase in the extension time (at 72°C) of 20 s in each subsequent cycle.

Article Title: trans-Complementation of Flavivirus RNA Polymerase Gene NS5 by Using Kunjin Virus Replicon-Expressing BHK Cells
Article Snippet: .. Therefore, we replaced the Sac II- Dra III (∼7 kb) fragment in the pAKUN clone (Fig. ) with the corresponding cDNA fragment obtained by RT of purified KUN virion RNA and PCR amplified with an Expand High Fidelity PCR kit (Boehringer Mannheim), using appropriate primers (see Materials and Methods). .. RNA transcribed from the resulting cDNA clone (FLSD) had a specific infectivity of ∼2 × 103 PFU per 1 μg, compared to only 1 to 5 PFU per 10 μg for AKUN RNA (Fig. ).

Hybridization:

Article Title: Biophysical Characterization of Fungal Phytases (myo-Inositol Hexakisphosphate Phosphohydrolases): Molecular Size, Glycosylation Pattern, and Engineering of Proteolytic Resistance
Article Snippet: .. The PCR was performed as described by the manufacturer (Expand High Fidelity PCR kit; Boehringer, Mannheim, Germany) by using a hybridization temperature of 55°C. .. The resulting PCR product was separated from the primers by using a PCR purification kit obtained from Qiagen (Hilden, Germany), was digested with Bam HI and Rca I, and was purified by agarose gel electrophoresis and a subsequent gel elution step (Qiaex II; Qiagen).

Amplification:

Article Title: Discrimination of Subtype B and Non-Subtype B Strains of Human Immunodeficiency Virus Type 1 by Serotyping: Correlation with Genotyping
Article Snippet: .. After stopping the reaction by putting it on ice, the cDNA was immediately amplified by PCR with an Expand High Fidelity PCR kit (catalog no. 1732650; Boehringer Mannheim). ..

Article Title: A Novel Phenotypic Drug Susceptibility Assay for Human Immunodeficiency Virus Type 1
Article Snippet: .. Reverse transcription was performed with Superscript II (Gibco/BRL, Gaithersburg, Md.) with an antisense internal primer, and PR and RT sequences were amplified with the Expand High Fidelity PCR kit (Boehringer Mannheim, Indianapolis, Ind.) with a forward primer containing an Apa I site and a reverse primer containing a Pin AI site ( ). .. The 1.5-kb amplification product spans the p7-p1-p6 protease cleavage sites in the gag polyprotein, the entire PR coding region, and the RT coding region from amino acids 1 to 313.

Article Title: trans-Complementation of Flavivirus RNA Polymerase Gene NS5 by Using Kunjin Virus Replicon-Expressing BHK Cells
Article Snippet: .. PCR amplification after RT of a 6,895-bp DNA fragment was performed with an Expand High Fidelity PCR kit (Boehringer Mannheim) and with a 1/25 to 1/10 volume of RT product as follows. .. The PCR mixture (50 μl) containing all necessary components except the enzyme mixture (3 parts Taq polymerase and 1 part Pwo polymerase) was preheated at 95°C for 5 min and then the enzyme mixture was added and the following cycles were performed: 10 cycles of 95°C for 15 s and 72°C for 6 min, followed by 6 cycles of 95°C for 15 s and 72°C for 6 min, with an automatic increase in the extension time (at 72°C) of 20 s in each subsequent cycle.

Article Title: trans-Complementation of Flavivirus RNA Polymerase Gene NS5 by Using Kunjin Virus Replicon-Expressing BHK Cells
Article Snippet: .. Therefore, we replaced the Sac II- Dra III (∼7 kb) fragment in the pAKUN clone (Fig. ) with the corresponding cDNA fragment obtained by RT of purified KUN virion RNA and PCR amplified with an Expand High Fidelity PCR kit (Boehringer Mannheim), using appropriate primers (see Materials and Methods). .. RNA transcribed from the resulting cDNA clone (FLSD) had a specific infectivity of ∼2 × 103 PFU per 1 μg, compared to only 1 to 5 PFU per 10 μg for AKUN RNA (Fig. ).

Purification:

Article Title: trans-Complementation of Flavivirus RNA Polymerase Gene NS5 by Using Kunjin Virus Replicon-Expressing BHK Cells
Article Snippet: .. Therefore, we replaced the Sac II- Dra III (∼7 kb) fragment in the pAKUN clone (Fig. ) with the corresponding cDNA fragment obtained by RT of purified KUN virion RNA and PCR amplified with an Expand High Fidelity PCR kit (Boehringer Mannheim), using appropriate primers (see Materials and Methods). .. RNA transcribed from the resulting cDNA clone (FLSD) had a specific infectivity of ∼2 × 103 PFU per 1 μg, compared to only 1 to 5 PFU per 10 μg for AKUN RNA (Fig. ).

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    Boehringer Mannheim expand high fidelity pcr kit
    Expand High Fidelity Pcr Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity pcr kit/product/Boehringer Mannheim
    Average 90 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr kit - by Bioz Stars, 2020-07
    90/100 stars
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