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Boehringer Mannheim expand high fidelity pcr kit
Expand High Fidelity Pcr Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expand high fidelity pcr kit/product/Boehringer Mannheim
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
expand high fidelity pcr kit - by Bioz Stars, 2021-07
86/100 stars

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Polymerase Chain Reaction:

Article Title: Spleen tyrosine kinase (Syk) deficiency in childhood pro-B cell acute lymphoblastic leukemia.
Article Snippet: P1 clones containing Syk genomic inserts were obtained from Research Genetics (Huntsville, AL, USA) after screening with the Syk cDNA probe. .. Ampli®cation from lambda phage or P1 DNA was performed using a mix of Taq and a proof-reading enzyme, Pwo polymerase, with either the `Expand High Fidelity System' (Boehringer-Mannheim, Indianapolis, IN, USA) or the `Expand Long Template PCR System' (Boehringer-Mannheim), according to manufacturer's recommendations. .. Amplification of the genomic DNA and genomic sequence analysis of the Syk gene Genomic DNA was isolated from a normal control EBV transformed lymphocyte cell line (approximately 46107 cells) with the PuregeneTM DNA isolation kit (Gentra Systems, Inc., Minneapolis, MN, USA) according to manufacturer's instructions.

Article Title: The olive DGAT2 gene is developmentally regulated and shares overlapping but distinct expression patterns with DGAT1
Article Snippet: cDNA cloning To amplify a central fragment of olive diacylglycerol acyltansferase type- 2 cDNA (OeDGAT2 ), the degenerate primers FOR1 (5′-C C/T TA C/T GT A/C/T TT C/T GG A/G/T TATGA A/G/ CC-3′) and REV2 (5′-CC A/G/C/T ACCACCAC A/G TG A/C/G/ AT A/T GG-3′) were designed for conserved regions of orthologous genes from GenBank. .. A cDNA amount corresponding to 150 ng of total RNA from mesocarp tissue at 25 weeks after flowering (WAF) was used as a template in PCR together with 200 μM of each dNTP, 20 pmol of each primer, 2 U of DNA polymerase (Expand™ High Fidelity, Boehringer, Mannheim) and 1× PCR buffer (provided by the manufacturer of the enzyme). .. Amplification was achieved in a thermal cycler (Model PTC-200, MJ Research, Waltham, MA) during 35 cycles of the following program: 94 °C for 30 s, 56 °C for 30 s, and 72 °C for 45 s. PCR products were gel extracted (QIAquick® Gel Extraction Kit), cloned into pGEM-T Easy (Promega) and sequenced by Macrogen Inc. (Seoul, Korea).

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    Boehringer Mannheim expand high fidelity pcr kit
    Expand High Fidelity Pcr Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity pcr kit/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr kit - by Bioz Stars, 2021-07
    86/100 stars
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