Structured Review

Boehringer Mannheim expand high fidelity pcr buffer
Expand High Fidelity Pcr Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expand high fidelity pcr buffer/product/Boehringer Mannheim
Average 88 stars, based on 6 article reviews
Price from $9.99 to $1999.99
expand high fidelity pcr buffer - by Bioz Stars, 2020-08
88/100 stars

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Polymerase Chain Reaction:

Article Title: Somatic Mutation of the Cd95 Gene in Human B Cells as a Side-Effect of the Germinal Center Reaction
Article Snippet: .. From six donors, single CD38+ CD77+ cells were sorted directly into PCR tubes containing 20 μl of 1× Expand High Fidelity PCR buffer (Boehringer Mannheim) on a FACS® 440 (Becton Dickinson). .. Tonsillar naive and memory B cells were isolated from the flowthrough fraction (CD77− cells).

Article Title: Frequent occurrence of deletions and duplications during somatic hypermutation: Implications for oncogene translocations and heavy chain disease
Article Snippet: .. On a FACS 440 (Becton Dickinson), single CD38+ Annexin V+ propidium iodide-negative cells were sorted into PCR tubes containing 20 μl of 1× Expand High Fidelity PCR buffer (Boehringer Mannheim). .. Single tonsillar GC centroblasts (CD77+ ) were enriched by magnetic cell separation (Miltenyi Biotec, Auburn, CA).

Article Title: Effect of Phenylurea Herbicides on Soil Microbial Communities Estimated by Analysis of 16S rRNA Gene Fingerprints and Community-Level Physiological Profiles
Article Snippet: .. The PCR mixture used contained 0.5 μM each primer, 100 μM each deoxynucleoside triphosphate, 10 μl of 10× Expand high-fidelity PCR buffer, 2 U of Expand high-fidelity DNA polymerase (Boehringer, Mannheim, Germany), 400 ng of bovine serum albumin (Boehringer) per μl, and sterile water to a final volume of 100 μl. .. The 16S rRNA genes from soil microbial communities were amplified by PCR with two different sets of primers.

Article Title: Human Immunoglobulin (Ig)M+IgD+ Peripheral Blood B Cells Expressing the CD27 Cell Surface Antigen Carry Somatically Mutated Variable Region Genes: CD27 as a General Marker for Somatically Mutated (Memory) B Cells
Article Snippet: .. After three washing steps, single IgD+ CD27+ and IgD+ CD27− B cells were sorted on a FACS® 440 ( Becton Dickinson ) directly into PCR tubes containing 20 μl Expand High Fidelity PCR buffer ( Boehringer Mannheim , Mannheim, Germany) and 20 ng 5S rRNA. .. Likewise, single IgM-only as well as IgD-only PB B cells were isolated from donor 3 after staining the CD19+ -enriched cell suspension against IgD and IgM as described above.

Article Title: Frequent occurrence of deletions and duplications during somatic hypermutation: Implications for oncogene translocations and heavy chain disease
Article Snippet: .. PCR was carried out in the same tube containing 1× Expand High Fidelity PCR buffer: 2.5 mM MgCl2 /100 μM each dATP, dGTP, dCTP, dTTP/2.8 nM each VH , Vκ , 3′JH , and 3′Jκ primer mix in a final volume of 50 μl. .. Expand High Fidelity Polymerase (1.75 U; Boehringer Mannheim) was added during the first primer annealing step.

Article Title: A catalytic antioxidant metalloporphyrin blocks hydrogen peroxide-induced mitochondrial DNA damage
Article Snippet: .. The final concentrations in the 50 µl amplification cocktail were 1× Expand High Fidelity PCR buffer (Boehringer-Mannheim), 300 nM each primer, 1.5 mM MgCl2 , 200 µM each dNTP and 2.6 U of a mixture of Taq and Pwo DNA polymerases. .. The PCR cycling profile used for the comparison of different template isolation methods was a pre-PCR incubation at 94°C for 2 min, then 10 cycles of 94°C for 15 s, 50°C for 30 s and 68°C for 4 min, which was followed by 15 cycles of 94°C for 15 s, 50°C for 30 s and 68°C for 4 min, with a 20 s extension per cycle and then to 4°C.

FACS:

Article Title: Somatic Mutation of the Cd95 Gene in Human B Cells as a Side-Effect of the Germinal Center Reaction
Article Snippet: .. From six donors, single CD38+ CD77+ cells were sorted directly into PCR tubes containing 20 μl of 1× Expand High Fidelity PCR buffer (Boehringer Mannheim) on a FACS® 440 (Becton Dickinson). .. Tonsillar naive and memory B cells were isolated from the flowthrough fraction (CD77− cells).

Article Title: Frequent occurrence of deletions and duplications during somatic hypermutation: Implications for oncogene translocations and heavy chain disease
Article Snippet: .. On a FACS 440 (Becton Dickinson), single CD38+ Annexin V+ propidium iodide-negative cells were sorted into PCR tubes containing 20 μl of 1× Expand High Fidelity PCR buffer (Boehringer Mannheim). .. Single tonsillar GC centroblasts (CD77+ ) were enriched by magnetic cell separation (Miltenyi Biotec, Auburn, CA).

Article Title: Human Immunoglobulin (Ig)M+IgD+ Peripheral Blood B Cells Expressing the CD27 Cell Surface Antigen Carry Somatically Mutated Variable Region Genes: CD27 as a General Marker for Somatically Mutated (Memory) B Cells
Article Snippet: .. After three washing steps, single IgD+ CD27+ and IgD+ CD27− B cells were sorted on a FACS® 440 ( Becton Dickinson ) directly into PCR tubes containing 20 μl Expand High Fidelity PCR buffer ( Boehringer Mannheim , Mannheim, Germany) and 20 ng 5S rRNA. .. Likewise, single IgM-only as well as IgD-only PB B cells were isolated from donor 3 after staining the CD19+ -enriched cell suspension against IgD and IgM as described above.

Amplification:

Article Title: A catalytic antioxidant metalloporphyrin blocks hydrogen peroxide-induced mitochondrial DNA damage
Article Snippet: .. The final concentrations in the 50 µl amplification cocktail were 1× Expand High Fidelity PCR buffer (Boehringer-Mannheim), 300 nM each primer, 1.5 mM MgCl2 , 200 µM each dNTP and 2.6 U of a mixture of Taq and Pwo DNA polymerases. .. The PCR cycling profile used for the comparison of different template isolation methods was a pre-PCR incubation at 94°C for 2 min, then 10 cycles of 94°C for 15 s, 50°C for 30 s and 68°C for 4 min, which was followed by 15 cycles of 94°C for 15 s, 50°C for 30 s and 68°C for 4 min, with a 20 s extension per cycle and then to 4°C.

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    Boehringer Mannheim expand high fidelity pcr buffer
    Expand High Fidelity Pcr Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity pcr buffer/product/Boehringer Mannheim
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr buffer - by Bioz Stars, 2020-08
    88/100 stars
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