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GE Healthcare exosap
Exosap, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/exosap/product/GE Healthcare
Average 93 stars, based on 46 article reviews
Price from $9.99 to $1999.99
exosap - by Bioz Stars, 2020-08
93/100 stars

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Amplification:

Article Title: Mutations in SCG10 Are Not Involved in Hirschsprung Disease
Article Snippet: .. PCR products were amplified and purified (ExoSap it – GE Healthcare). .. Direct sequencing of exons 1–5 was performed using the 3730 DNA Analyser (Applied Biosystems).

Purification:

Article Title: Genetic screening of Congenital Short Bowel Syndrome patients confirms CLMP as the major gene involved in the recessive form of this disorder
Article Snippet: .. PCR products were purified (ExoSap it – GE Healthcare, Eindhoven, The Netherlands), and Sanger sequencing was performed with dye labelled primers (forward and reverse; Big Dye Terminator v3.1 Sequencing Kit, Applied Biosystems, Bleiswijk, The Netherlands) on an ABI 3130XL genetic analyzer. .. Sanger reads were analysed using SeqScape software and compared to the reference CLMP genomic sequence (ENSG00000166250).

Article Title: Ancient and Contemporary DNA Reveal a Pre-Human Decline but No Population Bottleneck Associated with Recent Human Persecution in the Kea (Nestor notabilis)
Article Snippet: .. PCR products were purified (using ExoSap (1.5 U ExoI, I U SAP; GE Healthcare) by incubation at 37°C for 30 min and 80°C for 15 min), and sequenced bi-directionally from independent PCR products using Big Dye Terminator technology and an ABI 3730xl. .. When an inconsistency between sequences from an individual was observed due to DNA damage (C-T and G-A transitions), additional PCRs and bi-directional sequencing were conducted, and a majority rule consensus applied to the four independent PCRs [ ].

Article Title: Mutations in SCG10 Are Not Involved in Hirschsprung Disease
Article Snippet: .. PCR products were amplified and purified (ExoSap it – GE Healthcare). .. Direct sequencing of exons 1–5 was performed using the 3730 DNA Analyser (Applied Biosystems).

Article Title: New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.)
Article Snippet: .. All products with an insert size range of 300 – 1100 bp were purified for sequencing using EXOSAP (Amersham Biosciences, Freiburg, Germany). ..

Electrophoresis:

Article Title: Sequence Diversity in the Glycoprotein B Gene Complicates Real-Time PCR Assays for Detection and Quantification of Cytomegalovirus
Article Snippet: .. The amplicons were treated with ExoSap (Amersham Biosciences, Piscataway, NJ) to remove the primers and deoxynucleoside triphosphates and sequenced using the PCR primers as sequencing primers and Applied Biosystems (ABI, Foster City, CA) dye terminator chemistry, and then the sequencing reactions were resolved by capillary electrophoresis on the ABI 3100 Prism DNA sequencer. ..

Polymerase Chain Reaction:

Article Title: Phylogeography of the Golden Jackal (Canis aureus) in India
Article Snippet: .. PCR products were cleaned using ExoSAP (GE Healthcare, USA) and sequenced in both directions using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems Inc., USA) according to the manufacturer’s instructions. .. DNA sequences were resolved on the ABI Prism 3130 Genetic Analyzer (Applied Biosystems Inc., USA).

Article Title: Genetic screening of Congenital Short Bowel Syndrome patients confirms CLMP as the major gene involved in the recessive form of this disorder
Article Snippet: .. PCR products were purified (ExoSap it – GE Healthcare, Eindhoven, The Netherlands), and Sanger sequencing was performed with dye labelled primers (forward and reverse; Big Dye Terminator v3.1 Sequencing Kit, Applied Biosystems, Bleiswijk, The Netherlands) on an ABI 3130XL genetic analyzer. .. Sanger reads were analysed using SeqScape software and compared to the reference CLMP genomic sequence (ENSG00000166250).

Article Title: Ancient and Contemporary DNA Reveal a Pre-Human Decline but No Population Bottleneck Associated with Recent Human Persecution in the Kea (Nestor notabilis)
Article Snippet: .. PCR products were purified (using ExoSap (1.5 U ExoI, I U SAP; GE Healthcare) by incubation at 37°C for 30 min and 80°C for 15 min), and sequenced bi-directionally from independent PCR products using Big Dye Terminator technology and an ABI 3730xl. .. When an inconsistency between sequences from an individual was observed due to DNA damage (C-T and G-A transitions), additional PCRs and bi-directional sequencing were conducted, and a majority rule consensus applied to the four independent PCRs [ ].

Article Title: Homozygosity mapping reveals new nonsense mutation in the FAM161A gene causing autosomal recessive retinitis pigmentosa in a Palestinian family
Article Snippet: .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany). .. All sequences were run on an ABI 3100 DNA sequencer, processed with the Sequence Analysis 5.1 software (Applied Biosystems) and aligned with the reference sequence applying SeqMan (Lasergene, Madison WI, USA).

Article Title: Sequence Diversity in the Glycoprotein B Gene Complicates Real-Time PCR Assays for Detection and Quantification of Cytomegalovirus
Article Snippet: .. The amplicons were treated with ExoSap (Amersham Biosciences, Piscataway, NJ) to remove the primers and deoxynucleoside triphosphates and sequenced using the PCR primers as sequencing primers and Applied Biosystems (ABI, Foster City, CA) dye terminator chemistry, and then the sequencing reactions were resolved by capillary electrophoresis on the ABI 3100 Prism DNA sequencer. ..

Article Title: Mutations in SCG10 Are Not Involved in Hirschsprung Disease
Article Snippet: .. PCR products were amplified and purified (ExoSap it – GE Healthcare). .. Direct sequencing of exons 1–5 was performed using the 3730 DNA Analyser (Applied Biosystems).

Incubation:

Article Title: Ancient and Contemporary DNA Reveal a Pre-Human Decline but No Population Bottleneck Associated with Recent Human Persecution in the Kea (Nestor notabilis)
Article Snippet: .. PCR products were purified (using ExoSap (1.5 U ExoI, I U SAP; GE Healthcare) by incubation at 37°C for 30 min and 80°C for 15 min), and sequenced bi-directionally from independent PCR products using Big Dye Terminator technology and an ABI 3730xl. .. When an inconsistency between sequences from an individual was observed due to DNA damage (C-T and G-A transitions), additional PCRs and bi-directional sequencing were conducted, and a majority rule consensus applied to the four independent PCRs [ ].

DNA Sequencing:

Article Title: Homozygosity mapping reveals new nonsense mutation in the FAM161A gene causing autosomal recessive retinitis pigmentosa in a Palestinian family
Article Snippet: .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany). .. All sequences were run on an ABI 3100 DNA sequencer, processed with the Sequence Analysis 5.1 software (Applied Biosystems) and aligned with the reference sequence applying SeqMan (Lasergene, Madison WI, USA).

Article Title: Immunohistochemical detection of mutations in the epidermal growth factor receptor gene in lung adenocarcinomas using mutation-specific antibodies
Article Snippet: .. DNA sequencing for EGFR exon 19 and 21 The amplicons were treated with ExoSap (Amersham Biosciences, Piscataway, NJ) to remove the primers and dNTPs; then 1 μL was sequenced using the M13 tail primers as sequencing primers and Applied Biosystems (ABI, Foster City, CA) BigDye Terminator v.3.1 chemistry. .. The sequencing reactions were purified using the CleanSeq system (Agencourt Bioscience, Beverly, MA) and then resolved by capillary electrophoresis on the ABI 3100 Prism Genetic Analyzer.

Sequencing:

Article Title: Phylogeography of the Golden Jackal (Canis aureus) in India
Article Snippet: .. PCR products were cleaned using ExoSAP (GE Healthcare, USA) and sequenced in both directions using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems Inc., USA) according to the manufacturer’s instructions. .. DNA sequences were resolved on the ABI Prism 3130 Genetic Analyzer (Applied Biosystems Inc., USA).

Article Title: Genetic screening of Congenital Short Bowel Syndrome patients confirms CLMP as the major gene involved in the recessive form of this disorder
Article Snippet: .. PCR products were purified (ExoSap it – GE Healthcare, Eindhoven, The Netherlands), and Sanger sequencing was performed with dye labelled primers (forward and reverse; Big Dye Terminator v3.1 Sequencing Kit, Applied Biosystems, Bleiswijk, The Netherlands) on an ABI 3130XL genetic analyzer. .. Sanger reads were analysed using SeqScape software and compared to the reference CLMP genomic sequence (ENSG00000166250).

Article Title: Immunohistochemical detection of mutations in the epidermal growth factor receptor gene in lung adenocarcinomas using mutation-specific antibodies
Article Snippet: .. DNA sequencing for EGFR exon 19 and 21 The amplicons were treated with ExoSap (Amersham Biosciences, Piscataway, NJ) to remove the primers and dNTPs; then 1 μL was sequenced using the M13 tail primers as sequencing primers and Applied Biosystems (ABI, Foster City, CA) BigDye Terminator v.3.1 chemistry. .. The sequencing reactions were purified using the CleanSeq system (Agencourt Bioscience, Beverly, MA) and then resolved by capillary electrophoresis on the ABI 3100 Prism Genetic Analyzer.

Article Title: Sequence Diversity in the Glycoprotein B Gene Complicates Real-Time PCR Assays for Detection and Quantification of Cytomegalovirus
Article Snippet: .. The amplicons were treated with ExoSap (Amersham Biosciences, Piscataway, NJ) to remove the primers and deoxynucleoside triphosphates and sequenced using the PCR primers as sequencing primers and Applied Biosystems (ABI, Foster City, CA) dye terminator chemistry, and then the sequencing reactions were resolved by capillary electrophoresis on the ABI 3100 Prism DNA sequencer. ..

Article Title: New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.)
Article Snippet: .. All products with an insert size range of 300 – 1100 bp were purified for sequencing using EXOSAP (Amersham Biosciences, Freiburg, Germany). ..

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  • 90
    GE Healthcare exosap it kit
    Exosap It Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exosap it kit/product/GE Healthcare
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    GE Healthcare exosap it protocol
    Exosap It Protocol, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare exosap it enzyme kit
    Exosap It Enzyme Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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