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GE Healthcare exosap
Exosap, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 34 article reviews
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exosap - by Bioz Stars, 2020-01
96/100 stars

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Clone Assay:

Article Title: Generation and analysis of expressed sequence tags from six developing xylem libraries in Pinus radiata D. Don
Article Snippet: Approximately 8,000 sequencing reactions were performed on a total of 7,200 cDNA clones. .. The cDNA inserts were PCR amplified using the M13 forward and reverse primers followed by clean-up with ExoSap (GE Healthcare, UK).

Article Title: New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.)
Article Snippet: The selected fragments were once more amplified by PCR (20 cycles), purified using the QIAquick columns (Qiagen) and concentrations adjusted to approximately 25 ng μl-1 before cloning into pGEM-T vector (Promega, Madison, USA) according to the supplier's instructions. .. All products with an insert size range of 300 – 1100 bp were purified for sequencing using EXOSAP (Amersham Biosciences, Freiburg, Germany).

Amplification:

Article Title: Phylogeography of the Golden Jackal (Canis aureus) in India
Article Snippet: We amplified approximately 440 bp region of the mtDNA CR locus using the primer pairs ThrL15926 and DL-H16340 [ ]; and a 412 bp fragment of the mtDNA cyt b gene using a canid specific light primer (Canid L1, [ ]) and universal heavy primer (H15149, [ ]). .. PCR products were cleaned using ExoSAP (GE Healthcare, USA) and sequenced in both directions using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems Inc., USA) according to the manufacturer’s instructions.

Article Title: Adaptation of Subtype A Human Immunodeficiency Virus Type 1 Envelope to Pig-Tailed Macaque Cells ▿
Article Snippet: To sequence viral variants, 1,000 copies of the integrated provirus were amplified with four nested PCRs, resulting in overlapping amplicons spanning the entirety of the HIV-1 genome. .. All reaction mixtures were treated with ExoSap (Amersham Biosciences), and the amplicons were sequenced directly without gel purification.

Article Title: Index case of acute myeloid leukemia in a family harboring a novel CEBPA germ line mutation
Article Snippet: The region around codon 442 was amplified using polymerase chain reaction (PCR) in a total volume of 12 μL with primers at a final concentration of 1 μM each, 50 μM of each deoxynucleotide triphosphate, 10% dimethyl sulfoxide, 0.75 U of HotStar Taq DNA polymerase, 1.2 μL of the 10× buffer provided by the enzyme manufacturer (Qiagen Inc), and 50 ng of genomic DNA. .. The PCR products were treated with ExoSap (Amersham Biosciences) to remove the primers and deoxynucleotide triphosphates, then sequenced using the M13 tails as sequencing primers and Applied Biosystems BigDye Terminator v.3.1 chemistry.

Article Title: Genetic screening of Congenital Short Bowel Syndrome patients confirms CLMP as the major gene involved in the recessive form of this disorder
Article Snippet: Exons 1-7 of CLMP (ENST00000448775) were amplified using 30 ng of genomic DNA, as described before . .. PCR products were purified (ExoSap it – GE Healthcare, Eindhoven, The Netherlands), and Sanger sequencing was performed with dye labelled primers (forward and reverse; Big Dye Terminator v3.1 Sequencing Kit, Applied Biosystems, Bleiswijk, The Netherlands) on an ABI 3130XL genetic analyzer.

Article Title: Strong Phylogeographic Structure in a Sedentary Seabird, the Stewart Island Shag (Leucocarbo chalconotus)
Article Snippet: PCR thermocycling conditions consisted of 94°C 9 min, 60 cycles 94°C 30 s, 50°C 45 s, 72°C 1 min, followed by a final extension of 72°C 10 min. PCR amplification and all downstream procedures were carried out in a modern genetics laboratory. .. PCR products were purified (using ExoSap (1.5 U ExoI, I U SAP; GE Healthcare) by incubation at 37°C for 30 min and 80°C for 15 min), and sequenced as above, except that bi-directional sequencing was conducted from independent PCR products.

Article Title: TERT promoter mutations and telomere length in adult malignant gliomas and recurrences
Article Snippet: .. Amplified products were purified with Exosap (GE Healthcare, Buckinghamshire, UK) to remove unused primer and were subjected to 35 cycles of sequencing reaction with a di-deoxy terminator kit and forward and reverse primers in separate reactions (BigDyeTerminator v3.1 Cycle Sequencing Kit, Applied Biosystems, Austin, TX, USA). .. Sequencing reaction products were precipitated with ethanol and analyzed on a capillary sequencer (AbiPrism 3130xl Genetic Analyzer).

Article Title: CNGA3 mutations in two United Arab Emirates families with achromatopsia
Article Snippet: All coding exons and flanking intronic sequences of CNGA3 were amplified from genomic DNA by means of PCR [ ]. .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subjected to direct DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany).

Article Title: A CTNNA3 compound heterozygous deletion implicates a role for αT-catenin in susceptibility to autism spectrum disorder
Article Snippet: Primer sequences and PCR conditions used for amplification (20 amplicons for CTNNA3 and 5 amplicons for LRRTM3 ) are available on request. .. PCR products were purified using Exosap (GE Healthcare) and then sequenced using BigDye v1.1 (Life Technologies).

Article Title: Homozygosity mapping reveals new nonsense mutation in the FAM161A gene causing autosomal recessive retinitis pigmentosa in a Palestinian family
Article Snippet: Mutation screening of the FAM161A gene was done by complete DNA sequencing of all coding exons and flanking intron and UTR sequences from PCR amplified genomic DNA. .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany).

Article Title: BRAF Mutations in an Italian Regional Population: Implications for the Therapy of Thyroid Cancer
Article Snippet: .. The amplified PCR products were then treated with ExoSap (GE Healthcare, Waukesha, USA) as recommended and both strands sequenced by means of dye terminator cycle sequencing (BigDye Terminator v3.1, TFS). .. Nucleotide sequence detection was performed on an ABI Prism 3130 Genetic Analyzer (TFS), according to standard protocols.

Article Title: Generation and analysis of expressed sequence tags from six developing xylem libraries in Pinus radiata D. Don
Article Snippet: .. The cDNA inserts were PCR amplified using the M13 forward and reverse primers followed by clean-up with ExoSap (GE Healthcare, UK). .. The sequencing primer for the 5' end of cDNA inserts was the SK, T3 or M13 primer.

Article Title: A CTNNA3 compound heterozygous deletion implicates a role for αT-catenin in susceptibility to autism spectrum disorder
Article Snippet: Characterisation and segregation analysis of CTNNA3 deletions The maternal and the paternal exon 11 CTNNA3 microdeletions segregating in family 3456 were amplified via several polymerase chain reaction (PCR) assays using the Gold Taq polymerase (Life Technologies, Carlsbad, CA, USA). .. The paternal 301-bp and the maternal 949-bp deletion-spanning amplicons were purified using Exosap (GE Healthcare, Little Chalfont, UK) and then sequenced using BigDye Terminator kit v1.1 (Life Technologies) to determine the exact boundaries of the deletions.

Article Title: New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.)
Article Snippet: The selected fragments were once more amplified by PCR (20 cycles), purified using the QIAquick columns (Qiagen) and concentrations adjusted to approximately 25 ng μl-1 before cloning into pGEM-T vector (Promega, Madison, USA) according to the supplier's instructions. .. All products with an insert size range of 300 – 1100 bp were purified for sequencing using EXOSAP (Amersham Biosciences, Freiburg, Germany).

Synthesized:

Article Title: Index case of acute myeloid leukemia in a family harboring a novel CEBPA germ line mutation
Article Snippet: The PCR primers were synthesized (Integrated DNA Technologies) with M13 tail sequences appended to the 5′ end to facilitate sequencing. .. The PCR products were treated with ExoSap (Amersham Biosciences) to remove the primers and deoxynucleotide triphosphates, then sequenced using the M13 tails as sequencing primers and Applied Biosystems BigDye Terminator v.3.1 chemistry.

SYBR Green Assay:

Article Title: A CTNNA3 compound heterozygous deletion implicates a role for αT-catenin in susceptibility to autism spectrum disorder
Article Snippet: The paternal 301-bp and the maternal 949-bp deletion-spanning amplicons were purified using Exosap (GE Healthcare, Little Chalfont, UK) and then sequenced using BigDye Terminator kit v1.1 (Life Technologies) to determine the exact boundaries of the deletions. .. The experimental validation of CTNNA3 exonic deletions in four other ASD families was carried out by real-time quantitative PCR (qPCR) using Fast SYBR Green (Life Technologies).

Incubation:

Article Title: Strong Phylogeographic Structure in a Sedentary Seabird, the Stewart Island Shag (Leucocarbo chalconotus)
Article Snippet: .. PCR products were purified (using ExoSap (1.5 U ExoI, I U SAP; GE Healthcare) by incubation at 37°C for 30 min and 80°C for 15 min), and sequenced as above, except that bi-directional sequencing was conducted from independent PCR products. .. When an inconsistency between sequences from an individual was observed due to DNA damage (C-T and G-A transitions), additional PCRs and bi-directional sequencing were conducted, and a majority rule consensus was applied to the independent replicates .

Genome Wide:

Article Title: A CTNNA3 compound heterozygous deletion implicates a role for αT-catenin in susceptibility to autism spectrum disorder
Article Snippet: Primer pairs were designed following visual inspection of the Illumina 1 M-duo SNP array data from the second stage of the AGP genome-wide CNV study [ ], within GenomeStudio (Illumina, San Diego, CA, USA). .. The paternal 301-bp and the maternal 949-bp deletion-spanning amplicons were purified using Exosap (GE Healthcare, Little Chalfont, UK) and then sequenced using BigDye Terminator kit v1.1 (Life Technologies) to determine the exact boundaries of the deletions.

Real-time Polymerase Chain Reaction:

Article Title: Adaptation of Subtype A Human Immunodeficiency Virus Type 1 Envelope to Pig-Tailed Macaque Cells ▿
Article Snippet: The number of integrated proviral copies was determined using a previously described real-time PCR assay ( ). .. All reaction mixtures were treated with ExoSap (Amersham Biosciences), and the amplicons were sequenced directly without gel purification.

Article Title: BRAF Mutations in an Italian Regional Population: Implications for the Therapy of Thyroid Cancer
Article Snippet: Two methods were used to study the mutation: (i) conventional PCR followed by the direct Sanger sequencing method (according to the recommendations of the Italian Association of Medical Oncology (AIOM) and the Italian Society of Pathology and Cytology (SIAPEC)); and (ii) Real Time PCR (RT PCR) with commercial kits approved for clinical use (therascreen BRAF RGQ PCR, Qiagen). .. The amplified PCR products were then treated with ExoSap (GE Healthcare, Waukesha, USA) as recommended and both strands sequenced by means of dye terminator cycle sequencing (BigDye Terminator v3.1, TFS).

Article Title: A CTNNA3 compound heterozygous deletion implicates a role for αT-catenin in susceptibility to autism spectrum disorder
Article Snippet: The paternal 301-bp and the maternal 949-bp deletion-spanning amplicons were purified using Exosap (GE Healthcare, Little Chalfont, UK) and then sequenced using BigDye Terminator kit v1.1 (Life Technologies) to determine the exact boundaries of the deletions. .. The experimental validation of CTNNA3 exonic deletions in four other ASD families was carried out by real-time quantitative PCR (qPCR) using Fast SYBR Green (Life Technologies).

Hybridization:

Article Title: New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.)
Article Snippet: Microsatellite sequences were selected using biotinylated GA8 , GT8 , GAA8 and TAA8 oligonucleotide probes and streptavidin-coated magnetic beads following the hybridisation based capture methodology adapted from Billotte and co-workers [ ]. .. All products with an insert size range of 300 – 1100 bp were purified for sequencing using EXOSAP (Amersham Biosciences, Freiburg, Germany).

High Performance Liquid Chromatography:

Article Title: CNGA3 mutations in two United Arab Emirates families with achromatopsia
Article Snippet: The most common prevalent mutations in CNGB3 were excluded by PCR/RFLP and PCR/denaturating high pressure liquid chromatography (dHPLC) analysis in the index patient from each family, as described previously [ ]. .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subjected to direct DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany).

Ancient DNA Assay:

Article Title: Strong Phylogeographic Structure in a Sedentary Seabird, the Stewart Island Shag (Leucocarbo chalconotus)
Article Snippet: Paragraph title: Ancient DNA analysis ... PCR products were purified (using ExoSap (1.5 U ExoI, I U SAP; GE Healthcare) by incubation at 37°C for 30 min and 80°C for 15 min), and sequenced as above, except that bi-directional sequencing was conducted from independent PCR products.

Cell Culture:

Article Title: Index case of acute myeloid leukemia in a family harboring a novel CEBPA germ line mutation
Article Snippet: Genomic DNA was extracted from cultured fibroblasts using the QIAamp system (Qiagen Inc). .. The PCR products were treated with ExoSap (Amersham Biosciences) to remove the primers and deoxynucleotide triphosphates, then sequenced using the M13 tails as sequencing primers and Applied Biosystems BigDye Terminator v.3.1 chemistry.

Reverse Transcription Polymerase Chain Reaction:

Article Title: BRAF Mutations in an Italian Regional Population: Implications for the Therapy of Thyroid Cancer
Article Snippet: Two methods were used to study the mutation: (i) conventional PCR followed by the direct Sanger sequencing method (according to the recommendations of the Italian Association of Medical Oncology (AIOM) and the Italian Society of Pathology and Cytology (SIAPEC)); and (ii) Real Time PCR (RT PCR) with commercial kits approved for clinical use (therascreen BRAF RGQ PCR, Qiagen). .. The amplified PCR products were then treated with ExoSap (GE Healthcare, Waukesha, USA) as recommended and both strands sequenced by means of dye terminator cycle sequencing (BigDye Terminator v3.1, TFS).

Article Title: Expression of a hindlimb-determining factor Pitx1 in the forelimb of the lizard Pogona vitticeps during morphogenesis
Article Snippet: Paragraph title: RT-PCR ... The DNA was purified using ExoSAP (GE Healthcare, Ryldamere, NSW), according to the manufacturer's instructions, and sequenced by Macrogen (Korea).

DNA Sequencing:

Article Title: CNGA3 mutations in two United Arab Emirates families with achromatopsia
Article Snippet: .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subjected to direct DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany). .. All sequences were run on an ABI 3100 DNA sequencer, and analyzed with Sequence Analysis 5.1 (Applied Biosystems) and SeqMan software (Lasergene, Madison WI).

Article Title: Homozygosity mapping reveals new nonsense mutation in the FAM161A gene causing autosomal recessive retinitis pigmentosa in a Palestinian family
Article Snippet: .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany). .. All sequences were run on an ABI 3100 DNA sequencer, processed with the Sequence Analysis 5.1 software (Applied Biosystems) and aligned with the reference sequence applying SeqMan (Lasergene, Madison WI, USA).

Article Title: Immunohistochemical detection of mutations in the epidermal growth factor receptor gene in lung adenocarcinomas using mutation-specific antibodies
Article Snippet: .. DNA sequencing for EGFR exon 19 and 21 The amplicons were treated with ExoSap (Amersham Biosciences, Piscataway, NJ) to remove the primers and dNTPs; then 1 μL was sequenced using the M13 tail primers as sequencing primers and Applied Biosystems (ABI, Foster City, CA) BigDye Terminator v.3.1 chemistry. .. The sequencing reactions were purified using the CleanSeq system (Agencourt Bioscience, Beverly, MA) and then resolved by capillary electrophoresis on the ABI 3100 Prism Genetic Analyzer.

Sequencing:

Article Title: Phylogeography of the Golden Jackal (Canis aureus) in India
Article Snippet: .. PCR products were cleaned using ExoSAP (GE Healthcare, USA) and sequenced in both directions using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems Inc., USA) according to the manufacturer’s instructions. .. DNA sequences were resolved on the ABI Prism 3130 Genetic Analyzer (Applied Biosystems Inc., USA).

Article Title: Adaptation of Subtype A Human Immunodeficiency Virus Type 1 Envelope to Pig-Tailed Macaque Cells ▿
Article Snippet: Paragraph title: Long-term culturing of HIVAQ23 /SIVvif and sequencing of outgrowth variants. ... All reaction mixtures were treated with ExoSap (Amersham Biosciences), and the amplicons were sequenced directly without gel purification.

Article Title: Index case of acute myeloid leukemia in a family harboring a novel CEBPA germ line mutation
Article Snippet: .. The PCR products were treated with ExoSap (Amersham Biosciences) to remove the primers and deoxynucleotide triphosphates, then sequenced using the M13 tails as sequencing primers and Applied Biosystems BigDye Terminator v.3.1 chemistry. .. The sequencing reactions were purified using the CleanSeq system (Agencourt Bioscience) and then resolved by capillary electrophoresis on the ABI 3500XL Genetic Analyzer.

Article Title: Genetic screening of Congenital Short Bowel Syndrome patients confirms CLMP as the major gene involved in the recessive form of this disorder
Article Snippet: .. PCR products were purified (ExoSap it – GE Healthcare, Eindhoven, The Netherlands), and Sanger sequencing was performed with dye labelled primers (forward and reverse; Big Dye Terminator v3.1 Sequencing Kit, Applied Biosystems, Bleiswijk, The Netherlands) on an ABI 3130XL genetic analyzer. .. Sanger reads were analysed using SeqScape software and compared to the reference CLMP genomic sequence (ENSG00000166250).

Article Title: Strong Phylogeographic Structure in a Sedentary Seabird, the Stewart Island Shag (Leucocarbo chalconotus)
Article Snippet: .. PCR products were purified (using ExoSap (1.5 U ExoI, I U SAP; GE Healthcare) by incubation at 37°C for 30 min and 80°C for 15 min), and sequenced as above, except that bi-directional sequencing was conducted from independent PCR products. .. When an inconsistency between sequences from an individual was observed due to DNA damage (C-T and G-A transitions), additional PCRs and bi-directional sequencing were conducted, and a majority rule consensus was applied to the independent replicates .

Article Title: TERT promoter mutations and telomere length in adult malignant gliomas and recurrences
Article Snippet: .. Amplified products were purified with Exosap (GE Healthcare, Buckinghamshire, UK) to remove unused primer and were subjected to 35 cycles of sequencing reaction with a di-deoxy terminator kit and forward and reverse primers in separate reactions (BigDyeTerminator v3.1 Cycle Sequencing Kit, Applied Biosystems, Austin, TX, USA). .. Sequencing reaction products were precipitated with ethanol and analyzed on a capillary sequencer (AbiPrism 3130xl Genetic Analyzer).

Article Title: CNGA3 mutations in two United Arab Emirates families with achromatopsia
Article Snippet: PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subjected to direct DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany). .. All sequences were run on an ABI 3100 DNA sequencer, and analyzed with Sequence Analysis 5.1 (Applied Biosystems) and SeqMan software (Lasergene, Madison WI).

Article Title: A CTNNA3 compound heterozygous deletion implicates a role for αT-catenin in susceptibility to autism spectrum disorder
Article Snippet: Paragraph title: CTNNA3 and LRRTM3 exon sequencing ... PCR products were purified using Exosap (GE Healthcare) and then sequenced using BigDye v1.1 (Life Technologies).

Article Title: Homozygosity mapping reveals new nonsense mutation in the FAM161A gene causing autosomal recessive retinitis pigmentosa in a Palestinian family
Article Snippet: PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany). .. All sequences were run on an ABI 3100 DNA sequencer, processed with the Sequence Analysis 5.1 software (Applied Biosystems) and aligned with the reference sequence applying SeqMan (Lasergene, Madison WI, USA).

Article Title: BRAF Mutations in an Italian Regional Population: Implications for the Therapy of Thyroid Cancer
Article Snippet: .. The amplified PCR products were then treated with ExoSap (GE Healthcare, Waukesha, USA) as recommended and both strands sequenced by means of dye terminator cycle sequencing (BigDye Terminator v3.1, TFS). .. Nucleotide sequence detection was performed on an ABI Prism 3130 Genetic Analyzer (TFS), according to standard protocols.

Article Title: Generation and analysis of expressed sequence tags from six developing xylem libraries in Pinus radiata D. Don
Article Snippet: Paragraph title: EST sequencing and assembly ... The cDNA inserts were PCR amplified using the M13 forward and reverse primers followed by clean-up with ExoSap (GE Healthcare, UK).

Article Title: Immunohistochemical detection of mutations in the epidermal growth factor receptor gene in lung adenocarcinomas using mutation-specific antibodies
Article Snippet: .. DNA sequencing for EGFR exon 19 and 21 The amplicons were treated with ExoSap (Amersham Biosciences, Piscataway, NJ) to remove the primers and dNTPs; then 1 μL was sequenced using the M13 tail primers as sequencing primers and Applied Biosystems (ABI, Foster City, CA) BigDye Terminator v.3.1 chemistry. .. The sequencing reactions were purified using the CleanSeq system (Agencourt Bioscience, Beverly, MA) and then resolved by capillary electrophoresis on the ABI 3100 Prism Genetic Analyzer.

Article Title: New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.)
Article Snippet: .. All products with an insert size range of 300 – 1100 bp were purified for sequencing using EXOSAP (Amersham Biosciences, Freiburg, Germany). ..

Gel Purification:

Article Title: Adaptation of Subtype A Human Immunodeficiency Virus Type 1 Envelope to Pig-Tailed Macaque Cells ▿
Article Snippet: .. All reaction mixtures were treated with ExoSap (Amersham Biosciences), and the amplicons were sequenced directly without gel purification. ..

Cellular Antioxidant Activity Assay:

Article Title: Homozygosity mapping reveals new nonsense mutation in the FAM161A gene causing autosomal recessive retinitis pigmentosa in a Palestinian family
Article Snippet: PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany). .. Assessment of the c.1003C > T/p.R335X mutation in controls was performed by pyrosequencing on a PyroMark Q96 instrument (Qiagen, Hilden, Germany) applying a custom developed SNP assay (PCR primers: 5’-AAT TTA TAG CAA GGG AGG AAC AGA-3’ and 5’-biotin-GAG GAA TGG GTC TGG CTT TAA-3’; sequencing primer: 5’-CAA GGG AGG AAC AGA AG-3’).

Nucleic Acid Electrophoresis:

Article Title: Adaptation of Subtype A Human Immunodeficiency Virus Type 1 Envelope to Pig-Tailed Macaque Cells ▿
Article Snippet: The nested PCRs were performed in triplicate, and the PCR products were detected as single prominent bands by gel electrophoresis. .. All reaction mixtures were treated with ExoSap (Amersham Biosciences), and the amplicons were sequenced directly without gel purification.

Article Title: Expression of a hindlimb-determining factor Pitx1 in the forelimb of the lizard Pogona vitticeps during morphogenesis
Article Snippet: The RT-PCR programme used for all samples consisted of 80°C for 2 min, then 40 cycles of 95°C for 30 s, 55°C for 1 min and 72°C for 1 min, with a final extension step at 72°C for 10 min. PCR products (12 µl) were visualized using gel electrophoresis on 1.2% TBE agarose gels with 6 µl SYBR safe stain. .. The DNA was purified using ExoSAP (GE Healthcare, Ryldamere, NSW), according to the manufacturer's instructions, and sequenced by Macrogen (Korea).

Magnetic Beads:

Article Title: New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.)
Article Snippet: Microsatellite sequences were selected using biotinylated GA8 , GT8 , GAA8 and TAA8 oligonucleotide probes and streptavidin-coated magnetic beads following the hybridisation based capture methodology adapted from Billotte and co-workers [ ]. .. All products with an insert size range of 300 – 1100 bp were purified for sequencing using EXOSAP (Amersham Biosciences, Freiburg, Germany).

Mutagenesis:

Article Title: Homozygosity mapping reveals new nonsense mutation in the FAM161A gene causing autosomal recessive retinitis pigmentosa in a Palestinian family
Article Snippet: Mutation screening of the FAM161A gene was done by complete DNA sequencing of all coding exons and flanking intron and UTR sequences from PCR amplified genomic DNA. .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany).

Article Title: BRAF Mutations in an Italian Regional Population: Implications for the Therapy of Thyroid Cancer
Article Snippet: Two methods were used to study the mutation: (i) conventional PCR followed by the direct Sanger sequencing method (according to the recommendations of the Italian Association of Medical Oncology (AIOM) and the Italian Society of Pathology and Cytology (SIAPEC)); and (ii) Real Time PCR (RT PCR) with commercial kits approved for clinical use (therascreen BRAF RGQ PCR, Qiagen). .. The amplified PCR products were then treated with ExoSap (GE Healthcare, Waukesha, USA) as recommended and both strands sequenced by means of dye terminator cycle sequencing (BigDye Terminator v3.1, TFS).

Isolation:

Article Title: Genetic screening of Congenital Short Bowel Syndrome patients confirms CLMP as the major gene involved in the recessive form of this disorder
Article Snippet: Genetic analysis Genomic DNA was isolated from peripheral blood lymphocytes using standard methods. .. PCR products were purified (ExoSap it – GE Healthcare, Eindhoven, The Netherlands), and Sanger sequencing was performed with dye labelled primers (forward and reverse; Big Dye Terminator v3.1 Sequencing Kit, Applied Biosystems, Bleiswijk, The Netherlands) on an ABI 3130XL genetic analyzer.

Purification:

Article Title: Index case of acute myeloid leukemia in a family harboring a novel CEBPA germ line mutation
Article Snippet: The PCR products were treated with ExoSap (Amersham Biosciences) to remove the primers and deoxynucleotide triphosphates, then sequenced using the M13 tails as sequencing primers and Applied Biosystems BigDye Terminator v.3.1 chemistry. .. The sequencing reactions were purified using the CleanSeq system (Agencourt Bioscience) and then resolved by capillary electrophoresis on the ABI 3500XL Genetic Analyzer.

Article Title: Genetic screening of Congenital Short Bowel Syndrome patients confirms CLMP as the major gene involved in the recessive form of this disorder
Article Snippet: .. PCR products were purified (ExoSap it – GE Healthcare, Eindhoven, The Netherlands), and Sanger sequencing was performed with dye labelled primers (forward and reverse; Big Dye Terminator v3.1 Sequencing Kit, Applied Biosystems, Bleiswijk, The Netherlands) on an ABI 3130XL genetic analyzer. .. Sanger reads were analysed using SeqScape software and compared to the reference CLMP genomic sequence (ENSG00000166250).

Article Title: Strong Phylogeographic Structure in a Sedentary Seabird, the Stewart Island Shag (Leucocarbo chalconotus)
Article Snippet: .. PCR products were purified (using ExoSap (1.5 U ExoI, I U SAP; GE Healthcare) by incubation at 37°C for 30 min and 80°C for 15 min), and sequenced as above, except that bi-directional sequencing was conducted from independent PCR products. .. When an inconsistency between sequences from an individual was observed due to DNA damage (C-T and G-A transitions), additional PCRs and bi-directional sequencing were conducted, and a majority rule consensus was applied to the independent replicates .

Article Title: TERT promoter mutations and telomere length in adult malignant gliomas and recurrences
Article Snippet: .. Amplified products were purified with Exosap (GE Healthcare, Buckinghamshire, UK) to remove unused primer and were subjected to 35 cycles of sequencing reaction with a di-deoxy terminator kit and forward and reverse primers in separate reactions (BigDyeTerminator v3.1 Cycle Sequencing Kit, Applied Biosystems, Austin, TX, USA). .. Sequencing reaction products were precipitated with ethanol and analyzed on a capillary sequencer (AbiPrism 3130xl Genetic Analyzer).

Article Title: A CTNNA3 compound heterozygous deletion implicates a role for αT-catenin in susceptibility to autism spectrum disorder
Article Snippet: .. PCR products were purified using Exosap (GE Healthcare) and then sequenced using BigDye v1.1 (Life Technologies). ..

Article Title: Homozygosity mapping reveals new nonsense mutation in the FAM161A gene causing autosomal recessive retinitis pigmentosa in a Palestinian family
Article Snippet: PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany). .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany).

Article Title: Immunohistochemical detection of mutations in the epidermal growth factor receptor gene in lung adenocarcinomas using mutation-specific antibodies
Article Snippet: DNA sequencing for EGFR exon 19 and 21 The amplicons were treated with ExoSap (Amersham Biosciences, Piscataway, NJ) to remove the primers and dNTPs; then 1 μL was sequenced using the M13 tail primers as sequencing primers and Applied Biosystems (ABI, Foster City, CA) BigDye Terminator v.3.1 chemistry. .. The sequencing reactions were purified using the CleanSeq system (Agencourt Bioscience, Beverly, MA) and then resolved by capillary electrophoresis on the ABI 3100 Prism Genetic Analyzer.

Article Title: A CTNNA3 compound heterozygous deletion implicates a role for αT-catenin in susceptibility to autism spectrum disorder
Article Snippet: .. The paternal 301-bp and the maternal 949-bp deletion-spanning amplicons were purified using Exosap (GE Healthcare, Little Chalfont, UK) and then sequenced using BigDye Terminator kit v1.1 (Life Technologies) to determine the exact boundaries of the deletions. ..

Article Title: Expression of a hindlimb-determining factor Pitx1 in the forelimb of the lizard Pogona vitticeps during morphogenesis
Article Snippet: .. The DNA was purified using ExoSAP (GE Healthcare, Ryldamere, NSW), according to the manufacturer's instructions, and sequenced by Macrogen (Korea). .. A BLAST search ( http://www.ncbi.nlm.nih.gov/blast/Blast.cgi ) confirmed that the correct gene had been amplified.

Article Title: New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.)
Article Snippet: .. All products with an insert size range of 300 – 1100 bp were purified for sequencing using EXOSAP (Amersham Biosciences, Freiburg, Germany). ..

Polymerase Chain Reaction:

Article Title: Phylogeography of the Golden Jackal (Canis aureus) in India
Article Snippet: .. PCR products were cleaned using ExoSAP (GE Healthcare, USA) and sequenced in both directions using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems Inc., USA) according to the manufacturer’s instructions. .. DNA sequences were resolved on the ABI Prism 3130 Genetic Analyzer (Applied Biosystems Inc., USA).

Article Title: Adaptation of Subtype A Human Immunodeficiency Virus Type 1 Envelope to Pig-Tailed Macaque Cells ▿
Article Snippet: The nested PCRs were performed in triplicate, and the PCR products were detected as single prominent bands by gel electrophoresis. .. All reaction mixtures were treated with ExoSap (Amersham Biosciences), and the amplicons were sequenced directly without gel purification.

Article Title: Index case of acute myeloid leukemia in a family harboring a novel CEBPA germ line mutation
Article Snippet: .. The PCR products were treated with ExoSap (Amersham Biosciences) to remove the primers and deoxynucleotide triphosphates, then sequenced using the M13 tails as sequencing primers and Applied Biosystems BigDye Terminator v.3.1 chemistry. .. The sequencing reactions were purified using the CleanSeq system (Agencourt Bioscience) and then resolved by capillary electrophoresis on the ABI 3500XL Genetic Analyzer.

Article Title: Genetic screening of Congenital Short Bowel Syndrome patients confirms CLMP as the major gene involved in the recessive form of this disorder
Article Snippet: .. PCR products were purified (ExoSap it – GE Healthcare, Eindhoven, The Netherlands), and Sanger sequencing was performed with dye labelled primers (forward and reverse; Big Dye Terminator v3.1 Sequencing Kit, Applied Biosystems, Bleiswijk, The Netherlands) on an ABI 3130XL genetic analyzer. .. Sanger reads were analysed using SeqScape software and compared to the reference CLMP genomic sequence (ENSG00000166250).

Article Title: Strong Phylogeographic Structure in a Sedentary Seabird, the Stewart Island Shag (Leucocarbo chalconotus)
Article Snippet: .. PCR products were purified (using ExoSap (1.5 U ExoI, I U SAP; GE Healthcare) by incubation at 37°C for 30 min and 80°C for 15 min), and sequenced as above, except that bi-directional sequencing was conducted from independent PCR products. .. When an inconsistency between sequences from an individual was observed due to DNA damage (C-T and G-A transitions), additional PCRs and bi-directional sequencing were conducted, and a majority rule consensus was applied to the independent replicates .

Article Title: TERT promoter mutations and telomere length in adult malignant gliomas and recurrences
Article Snippet: PCR was carried out in a 10 μl volume containing 10 ng DNA, 50 mM KCl, 0.11 mM dNTP and 0.11 mM of each primer. .. Amplified products were purified with Exosap (GE Healthcare, Buckinghamshire, UK) to remove unused primer and were subjected to 35 cycles of sequencing reaction with a di-deoxy terminator kit and forward and reverse primers in separate reactions (BigDyeTerminator v3.1 Cycle Sequencing Kit, Applied Biosystems, Austin, TX, USA).

Article Title: CNGA3 mutations in two United Arab Emirates families with achromatopsia
Article Snippet: .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subjected to direct DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany). .. All sequences were run on an ABI 3100 DNA sequencer, and analyzed with Sequence Analysis 5.1 (Applied Biosystems) and SeqMan software (Lasergene, Madison WI).

Article Title: A CTNNA3 compound heterozygous deletion implicates a role for αT-catenin in susceptibility to autism spectrum disorder
Article Snippet: .. PCR products were purified using Exosap (GE Healthcare) and then sequenced using BigDye v1.1 (Life Technologies). ..

Article Title: Homozygosity mapping reveals new nonsense mutation in the FAM161A gene causing autosomal recessive retinitis pigmentosa in a Palestinian family
Article Snippet: .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany). .. All sequences were run on an ABI 3100 DNA sequencer, processed with the Sequence Analysis 5.1 software (Applied Biosystems) and aligned with the reference sequence applying SeqMan (Lasergene, Madison WI, USA).

Article Title: BRAF Mutations in an Italian Regional Population: Implications for the Therapy of Thyroid Cancer
Article Snippet: .. The amplified PCR products were then treated with ExoSap (GE Healthcare, Waukesha, USA) as recommended and both strands sequenced by means of dye terminator cycle sequencing (BigDye Terminator v3.1, TFS). .. Nucleotide sequence detection was performed on an ABI Prism 3130 Genetic Analyzer (TFS), according to standard protocols.

Article Title: Generation and analysis of expressed sequence tags from six developing xylem libraries in Pinus radiata D. Don
Article Snippet: .. The cDNA inserts were PCR amplified using the M13 forward and reverse primers followed by clean-up with ExoSap (GE Healthcare, UK). .. The sequencing primer for the 5' end of cDNA inserts was the SK, T3 or M13 primer.

Article Title: A CTNNA3 compound heterozygous deletion implicates a role for αT-catenin in susceptibility to autism spectrum disorder
Article Snippet: Characterisation and segregation analysis of CTNNA3 deletions The maternal and the paternal exon 11 CTNNA3 microdeletions segregating in family 3456 were amplified via several polymerase chain reaction (PCR) assays using the Gold Taq polymerase (Life Technologies, Carlsbad, CA, USA). .. The paternal 301-bp and the maternal 949-bp deletion-spanning amplicons were purified using Exosap (GE Healthcare, Little Chalfont, UK) and then sequenced using BigDye Terminator kit v1.1 (Life Technologies) to determine the exact boundaries of the deletions.

Article Title: Expression of a hindlimb-determining factor Pitx1 in the forelimb of the lizard Pogona vitticeps during morphogenesis
Article Snippet: The RT-PCR programme used for all samples consisted of 80°C for 2 min, then 40 cycles of 95°C for 30 s, 55°C for 1 min and 72°C for 1 min, with a final extension step at 72°C for 10 min. PCR products (12 µl) were visualized using gel electrophoresis on 1.2% TBE agarose gels with 6 µl SYBR safe stain. .. The DNA was purified using ExoSAP (GE Healthcare, Ryldamere, NSW), according to the manufacturer's instructions, and sequenced by Macrogen (Korea).

Article Title: New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.)
Article Snippet: Positive colonies were handpicked and subjected to colony PCR using T7 and SP6 primers (Metabion, Martinsried, Germany). .. All products with an insert size range of 300 – 1100 bp were purified for sequencing using EXOSAP (Amersham Biosciences, Freiburg, Germany).

Multiplex Ligation-dependent Probe Amplification:

Article Title: TERT promoter mutations and telomere length in adult malignant gliomas and recurrences
Article Snippet: Mutations in IDH2 were confirmed by sequencing if the respective probes indicated a signal in MLPA. .. Amplified products were purified with Exosap (GE Healthcare, Buckinghamshire, UK) to remove unused primer and were subjected to 35 cycles of sequencing reaction with a di-deoxy terminator kit and forward and reverse primers in separate reactions (BigDyeTerminator v3.1 Cycle Sequencing Kit, Applied Biosystems, Austin, TX, USA).

Chromatin Immunoprecipitation:

Article Title: Homozygosity mapping reveals new nonsense mutation in the FAM161A gene causing autosomal recessive retinitis pigmentosa in a Palestinian family
Article Snippet: SNP chip genotypes were used for mapping of autozygous regions applying the online version of the Homozygosity Mapper software [ ]. .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany).

Plasmid Preparation:

Article Title: BRAF Mutations in an Italian Regional Population: Implications for the Therapy of Thyroid Cancer
Article Snippet: The amplified PCR products were then treated with ExoSap (GE Healthcare, Waukesha, USA) as recommended and both strands sequenced by means of dye terminator cycle sequencing (BigDye Terminator v3.1, TFS). .. The sequence data were analyzed by means of Mac Vector software version 11 (MacVector Inc., North Carolina, USA) in order to identify mutations and to assign genotypes to individual DNA samples.

Article Title: New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.)
Article Snippet: The selected fragments were once more amplified by PCR (20 cycles), purified using the QIAquick columns (Qiagen) and concentrations adjusted to approximately 25 ng μl-1 before cloning into pGEM-T vector (Promega, Madison, USA) according to the supplier's instructions. .. All products with an insert size range of 300 – 1100 bp were purified for sequencing using EXOSAP (Amersham Biosciences, Freiburg, Germany).

Software:

Article Title: Genetic screening of Congenital Short Bowel Syndrome patients confirms CLMP as the major gene involved in the recessive form of this disorder
Article Snippet: PCR products were purified (ExoSap it – GE Healthcare, Eindhoven, The Netherlands), and Sanger sequencing was performed with dye labelled primers (forward and reverse; Big Dye Terminator v3.1 Sequencing Kit, Applied Biosystems, Bleiswijk, The Netherlands) on an ABI 3130XL genetic analyzer. .. Sanger reads were analysed using SeqScape software and compared to the reference CLMP genomic sequence (ENSG00000166250).

Article Title: TERT promoter mutations and telomere length in adult malignant gliomas and recurrences
Article Snippet: Amplified products were purified with Exosap (GE Healthcare, Buckinghamshire, UK) to remove unused primer and were subjected to 35 cycles of sequencing reaction with a di-deoxy terminator kit and forward and reverse primers in separate reactions (BigDyeTerminator v3.1 Cycle Sequencing Kit, Applied Biosystems, Austin, TX, USA). .. The sequencing data were analyzed using Geneious Pro 5.6.5 software with reference to the sequences from the NCBI gene database, TERT (chr5: 1, 295, 071–1, 295, 521), IDH1 (NC_000002.12) and IDH2 (NC_000015.).

Article Title: CNGA3 mutations in two United Arab Emirates families with achromatopsia
Article Snippet: PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subjected to direct DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany). .. All sequences were run on an ABI 3100 DNA sequencer, and analyzed with Sequence Analysis 5.1 (Applied Biosystems) and SeqMan software (Lasergene, Madison WI).

Article Title: Homozygosity mapping reveals new nonsense mutation in the FAM161A gene causing autosomal recessive retinitis pigmentosa in a Palestinian family
Article Snippet: SNP chip genotypes were used for mapping of autozygous regions applying the online version of the Homozygosity Mapper software [ ]. .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany).

Article Title: BRAF Mutations in an Italian Regional Population: Implications for the Therapy of Thyroid Cancer
Article Snippet: The amplified PCR products were then treated with ExoSap (GE Healthcare, Waukesha, USA) as recommended and both strands sequenced by means of dye terminator cycle sequencing (BigDye Terminator v3.1, TFS). .. The sequence data were analyzed by means of Mac Vector software version 11 (MacVector Inc., North Carolina, USA) in order to identify mutations and to assign genotypes to individual DNA samples.

Electrophoresis:

Article Title: Index case of acute myeloid leukemia in a family harboring a novel CEBPA germ line mutation
Article Snippet: The PCR products were treated with ExoSap (Amersham Biosciences) to remove the primers and deoxynucleotide triphosphates, then sequenced using the M13 tails as sequencing primers and Applied Biosystems BigDye Terminator v.3.1 chemistry. .. The sequencing reactions were purified using the CleanSeq system (Agencourt Bioscience) and then resolved by capillary electrophoresis on the ABI 3500XL Genetic Analyzer.

Article Title: Immunohistochemical detection of mutations in the epidermal growth factor receptor gene in lung adenocarcinomas using mutation-specific antibodies
Article Snippet: DNA sequencing for EGFR exon 19 and 21 The amplicons were treated with ExoSap (Amersham Biosciences, Piscataway, NJ) to remove the primers and dNTPs; then 1 μL was sequenced using the M13 tail primers as sequencing primers and Applied Biosystems (ABI, Foster City, CA) BigDye Terminator v.3.1 chemistry. .. The sequencing reactions were purified using the CleanSeq system (Agencourt Bioscience, Beverly, MA) and then resolved by capillary electrophoresis on the ABI 3100 Prism Genetic Analyzer.

Negative Control:

Article Title: Expression of a hindlimb-determining factor Pitx1 in the forelimb of the lizard Pogona vitticeps during morphogenesis
Article Snippet: A negative control, using water instead of cDNA, was included in each RT-PCR. .. The DNA was purified using ExoSAP (GE Healthcare, Ryldamere, NSW), according to the manufacturer's instructions, and sequenced by Macrogen (Korea).

Cell Attachment Assay:

Article Title: Index case of acute myeloid leukemia in a family harboring a novel CEBPA germ line mutation
Article Snippet: Dissociated cells were centrifuged at 1000 rpm, transferred into a tissue-culture flask with complete Amniomax medium to allow cell attachment, and kept at 37°C in a CO2 incubator. .. The PCR products were treated with ExoSap (Amersham Biosciences) to remove the primers and deoxynucleotide triphosphates, then sequenced using the M13 tails as sequencing primers and Applied Biosystems BigDye Terminator v.3.1 chemistry.

Agarose Gel Electrophoresis:

Article Title: Strong Phylogeographic Structure in a Sedentary Seabird, the Stewart Island Shag (Leucocarbo chalconotus)
Article Snippet: PCR products were run on a 2% 1× TAE agarose gel. .. PCR products were purified (using ExoSap (1.5 U ExoI, I U SAP; GE Healthcare) by incubation at 37°C for 30 min and 80°C for 15 min), and sequenced as above, except that bi-directional sequencing was conducted from independent PCR products.

Sampling:

Article Title: Phylogeography of the Golden Jackal (Canis aureus) in India
Article Snippet: Paragraph title: Sampling and Laboratory work ... PCR products were cleaned using ExoSAP (GE Healthcare, USA) and sequenced in both directions using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems Inc., USA) according to the manufacturer’s instructions.

Concentration Assay:

Article Title: Index case of acute myeloid leukemia in a family harboring a novel CEBPA germ line mutation
Article Snippet: The region around codon 442 was amplified using polymerase chain reaction (PCR) in a total volume of 12 μL with primers at a final concentration of 1 μM each, 50 μM of each deoxynucleotide triphosphate, 10% dimethyl sulfoxide, 0.75 U of HotStar Taq DNA polymerase, 1.2 μL of the 10× buffer provided by the enzyme manufacturer (Qiagen Inc), and 50 ng of genomic DNA. .. The PCR products were treated with ExoSap (Amersham Biosciences) to remove the primers and deoxynucleotide triphosphates, then sequenced using the M13 tails as sequencing primers and Applied Biosystems BigDye Terminator v.3.1 chemistry.

DNA Purification:

Article Title: Homozygosity mapping reveals new nonsense mutation in the FAM161A gene causing autosomal recessive retinitis pigmentosa in a Palestinian family
Article Snippet: Genetic analysis Genomic DNA was prepared from peripheral venous blood using the MasterPure™ DNA Purification Kit (Epicentre, Madison, WI) and stored in TE buffer. .. PCR fragments were treated with ExoSAP (GE Healthcare, Freiburg, Germany) and subsequently subjected to DNA sequencing applying BigDye Terminator 1.1 chemistry (Applied Biosystems, Darmstadt, Germany).

Staining:

Article Title: Expression of a hindlimb-determining factor Pitx1 in the forelimb of the lizard Pogona vitticeps during morphogenesis
Article Snippet: The RT-PCR programme used for all samples consisted of 80°C for 2 min, then 40 cycles of 95°C for 30 s, 55°C for 1 min and 72°C for 1 min, with a final extension step at 72°C for 10 min. PCR products (12 µl) were visualized using gel electrophoresis on 1.2% TBE agarose gels with 6 µl SYBR safe stain. .. The DNA was purified using ExoSAP (GE Healthcare, Ryldamere, NSW), according to the manufacturer's instructions, and sequenced by Macrogen (Korea).

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