exosap pcr clean up reagent  (Thermo Fisher)


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    Name:
    ExoSAP IT PCR Product Cleanup Reagent
    Description:
    ExoSAP IT PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product It hydrolyzes excess primers and nucleotides in a single step ExoSAP IT purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism SNP analysis • Remove excess primers and unincorporated nucleotides enzymatically • Prepare DNA sequencing samples with one pipetting step • Conserve precious PCR products with up to 100 recovery • Minimize column cleanup and sample loss The ExoSAP IT one step solution provides a simple way to enzymatically purify a PCR reaction The reaction setup is complete with one pipetting step which is followed by two incubations The first incubation digests excess primer and dephosphorylates nucleotides The second high temperature incubation inactivates the enzymes Enzymatic cleanup is a very efficient method for PCR purification Adding ExoSAP IT reagent directly to the PCR product eliminates transfer steps to tubes wells or columns and conserves PCR amplicons helping reduce the chance of cross contamination no further processing needed After enzymatic cleanup with ExoSAP IT reagent primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis
    Catalog Number:
    78200.200.ul
    Price:
    None
    Applications:
    PCR|PCR & Real-Time PCR|Sequencing
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher exosap pcr clean up reagent
    ExoSAP IT PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product It hydrolyzes excess primers and nucleotides in a single step ExoSAP IT purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism SNP analysis • Remove excess primers and unincorporated nucleotides enzymatically • Prepare DNA sequencing samples with one pipetting step • Conserve precious PCR products with up to 100 recovery • Minimize column cleanup and sample loss The ExoSAP IT one step solution provides a simple way to enzymatically purify a PCR reaction The reaction setup is complete with one pipetting step which is followed by two incubations The first incubation digests excess primer and dephosphorylates nucleotides The second high temperature incubation inactivates the enzymes Enzymatic cleanup is a very efficient method for PCR purification Adding ExoSAP IT reagent directly to the PCR product eliminates transfer steps to tubes wells or columns and conserves PCR amplicons helping reduce the chance of cross contamination no further processing needed After enzymatic cleanup with ExoSAP IT reagent primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis
    https://www.bioz.com/result/exosap pcr clean up reagent/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exosap pcr clean up reagent - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Gene Genealogies Reveal High Nucleotide Diversity and Admixture Haplotypes within Three Alternaria Species Associated with Tomato and Potato.
    Article Snippet: .. We collected 152 isolates of three Alternaria spp. associated with tomato and potato in NC and WI and used the gene genealogical approach to compare the genetic relationships among them. .. We collected 152 isolates of three Alternaria spp. associated with tomato and potato in NC and WI and used the gene genealogical approach to compare the genetic relationships among them.

    Article Title: Novel digenic inheritance of PCDH15 and USH1G underlies profound non-syndromic hearing impairment
    Article Snippet: .. PCR amplified products were treated with ExoSAP-IT™ PCR Product Cleanup Reagent (ThermoFisher Scientific, Sugerland, TX) and sequenced using the BigDye terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA) on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA). ..

    Article Title: Novel TNXB Variants in Two Italian Patients with Classical-Like Ehlers-Danlos Syndrome
    Article Snippet: .. The amplified products were subsequently purified by using ExoSAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) and sequenced by using BigDye Terminator v1.1 sequencing kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The fragments obtained were purified using DyeEx plates (Qiagen, Hilden, Germany) and resolved on ABI Prism 3130 Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Prevalence of HIV, Hepatitis B and C Infections and an Assessment of HCV-Genotypes and Two IL28B SNPs among People Who Inject Drugs in Three Regions of Nepal
    Article Snippet: .. The PCR products of samples showing the expected product size were purified using the ExoSAP-IT PCR product clean up kit (Affymetrix, USA). .. The purified products were DNA sequenced using the Big Dye Terminator Version 3.1 Cycle Sequencing Kit (Applied Biosystems, USA) with the forward primer DM50.

    Article Title: A homozygous STIM1 mutation impairs store-operated calcium entry and natural killer cell effector function without clinical immunodeficiency
    Article Snippet: .. PCR product cleanup was performed with ExoSAP-IT (Affymetrix) before Sanger sequencing with the BigDye Terminator Cycle Sequencing Kit, version 3.1 (Applied Biosystems) and analysis on an ABI 3130XL DNA analyzer (Applied Biosystems). .. Flow cytometric analysis of calcium flux PBMCs were labeled with Dulbecco modified Eagle medium containing 5 μmol/L Indo-1 for 45 minutes at 37°C and then washed and cooled on ice.

    Article Title: New species of Cylindrocladiella from plantation soils in South-East Asia
    Article Snippet: .. Amplified fragments were purified using ExoSAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA). .. The products were sequenced in both directions with the same primers used for amplification, using the BigDye terminator sequencing kit v. 3.1 (Applied Biosystems, USA) on an ABI PRISM 3100 DNA sequencer (Applied Biosystems, USA).

    Article Title: High Whole-Genome Sequence Diversity of Human Papillomavirus Type 18 Isolates
    Article Snippet: .. Successfully generated amplicons were treated with ExoSAP-IT PCR product clean-up (Affymetrix, Santa Clara, CA, USA) according to manufacturer’s protocol. ..

    Article Title: Specifying the molecular pattern of sporadic parathyroid tumorigenesis—The Y282D variant of the GCM2 gene
    Article Snippet: .. The PCR product was purified from unused primers and nucleotides with ExoSAP-IT® PCR Product Cleanup reagent (Affymetrix Inc., Santa Clara, CA, USA). .. The single nucleotide polymorphisms were amplified using the fluorescent dideoxy chain termination method of cycle sequencing with color-tagged dNTPs and antisense primers according to the manufacturers instructions (Big Dye® Terminator, Applied Biosystems).

    Sequencing:

    Article Title: Novel digenic inheritance of PCDH15 and USH1G underlies profound non-syndromic hearing impairment
    Article Snippet: .. PCR amplified products were treated with ExoSAP-IT™ PCR Product Cleanup Reagent (ThermoFisher Scientific, Sugerland, TX) and sequenced using the BigDye terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA) on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA). ..

    Article Title: Novel TNXB Variants in Two Italian Patients with Classical-Like Ehlers-Danlos Syndrome
    Article Snippet: .. The amplified products were subsequently purified by using ExoSAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) and sequenced by using BigDye Terminator v1.1 sequencing kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The fragments obtained were purified using DyeEx plates (Qiagen, Hilden, Germany) and resolved on ABI Prism 3130 Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: A homozygous STIM1 mutation impairs store-operated calcium entry and natural killer cell effector function without clinical immunodeficiency
    Article Snippet: .. PCR product cleanup was performed with ExoSAP-IT (Affymetrix) before Sanger sequencing with the BigDye Terminator Cycle Sequencing Kit, version 3.1 (Applied Biosystems) and analysis on an ABI 3130XL DNA analyzer (Applied Biosystems). .. Flow cytometric analysis of calcium flux PBMCs were labeled with Dulbecco modified Eagle medium containing 5 μmol/L Indo-1 for 45 minutes at 37°C and then washed and cooled on ice.

    Generated:

    Article Title: High Whole-Genome Sequence Diversity of Human Papillomavirus Type 18 Isolates
    Article Snippet: .. Successfully generated amplicons were treated with ExoSAP-IT PCR product clean-up (Affymetrix, Santa Clara, CA, USA) according to manufacturer’s protocol. ..

    Amplification:

    Article Title: Novel digenic inheritance of PCDH15 and USH1G underlies profound non-syndromic hearing impairment
    Article Snippet: .. PCR amplified products were treated with ExoSAP-IT™ PCR Product Cleanup Reagent (ThermoFisher Scientific, Sugerland, TX) and sequenced using the BigDye terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA) on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA). ..

    Article Title: Novel TNXB Variants in Two Italian Patients with Classical-Like Ehlers-Danlos Syndrome
    Article Snippet: .. The amplified products were subsequently purified by using ExoSAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) and sequenced by using BigDye Terminator v1.1 sequencing kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The fragments obtained were purified using DyeEx plates (Qiagen, Hilden, Germany) and resolved on ABI Prism 3130 Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: New species of Cylindrocladiella from plantation soils in South-East Asia
    Article Snippet: .. Amplified fragments were purified using ExoSAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA). .. The products were sequenced in both directions with the same primers used for amplification, using the BigDye terminator sequencing kit v. 3.1 (Applied Biosystems, USA) on an ABI PRISM 3100 DNA sequencer (Applied Biosystems, USA).

    Purification:

    Article Title: Gene Genealogies Reveal High Nucleotide Diversity and Admixture Haplotypes within Three Alternaria Species Associated with Tomato and Potato.
    Article Snippet: .. We collected 152 isolates of three Alternaria spp. associated with tomato and potato in NC and WI and used the gene genealogical approach to compare the genetic relationships among them. .. We collected 152 isolates of three Alternaria spp. associated with tomato and potato in NC and WI and used the gene genealogical approach to compare the genetic relationships among them.

    Article Title: Novel TNXB Variants in Two Italian Patients with Classical-Like Ehlers-Danlos Syndrome
    Article Snippet: .. The amplified products were subsequently purified by using ExoSAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) and sequenced by using BigDye Terminator v1.1 sequencing kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The fragments obtained were purified using DyeEx plates (Qiagen, Hilden, Germany) and resolved on ABI Prism 3130 Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Prevalence of HIV, Hepatitis B and C Infections and an Assessment of HCV-Genotypes and Two IL28B SNPs among People Who Inject Drugs in Three Regions of Nepal
    Article Snippet: .. The PCR products of samples showing the expected product size were purified using the ExoSAP-IT PCR product clean up kit (Affymetrix, USA). .. The purified products were DNA sequenced using the Big Dye Terminator Version 3.1 Cycle Sequencing Kit (Applied Biosystems, USA) with the forward primer DM50.

    Article Title: New species of Cylindrocladiella from plantation soils in South-East Asia
    Article Snippet: .. Amplified fragments were purified using ExoSAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA). .. The products were sequenced in both directions with the same primers used for amplification, using the BigDye terminator sequencing kit v. 3.1 (Applied Biosystems, USA) on an ABI PRISM 3100 DNA sequencer (Applied Biosystems, USA).

    Article Title: Specifying the molecular pattern of sporadic parathyroid tumorigenesis—The Y282D variant of the GCM2 gene
    Article Snippet: .. The PCR product was purified from unused primers and nucleotides with ExoSAP-IT® PCR Product Cleanup reagent (Affymetrix Inc., Santa Clara, CA, USA). .. The single nucleotide polymorphisms were amplified using the fluorescent dideoxy chain termination method of cycle sequencing with color-tagged dNTPs and antisense primers according to the manufacturers instructions (Big Dye® Terminator, Applied Biosystems).

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  • 90
    Thermo Fisher exosap reagent
    Exosap Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exosap reagent/product/Thermo Fisher
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    exosap reagent - by Bioz Stars, 2020-09
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    99
    Thermo Fisher exosap pcr clean up reagent
    Exosap Pcr Clean Up Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exosap pcr clean up reagent/product/Thermo Fisher
    Average 99 stars, based on 86 article reviews
    Price from $9.99 to $1999.99
    exosap pcr clean up reagent - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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