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GE Healthcare exosap it pcr clean up reagent
Exosap It Pcr Clean Up Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/exosap it pcr clean up reagent/product/GE Healthcare
Average 94 stars, based on 3 article reviews
Price from $9.99 to $1999.99
exosap it pcr clean up reagent - by Bioz Stars, 2020-09
94/100 stars

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Amplification:

Article Title: DNA barcoding of the fire ant genus Solenopsis Westwood (Hymenoptera: Formicidae) from the Riyadh region, the Kingdom of Saudi Arabia
Article Snippet: .. In case of a single discrete amplified band, the PCR products were directly purified with FavorPrep™ MicroElute PCR clean up kit (GE Healthcare, Buckinghamshire, UK), whereas, in case of double bands, the required band was excised from the gel and purified with the same clean up kit. ..

Mouse Assay:

Article Title: In utero and lactational 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure exacerbates urinary dysfunction in hormone-treated C57BL/6J mice through a non-malignant mechanism involving proteomic changes in the prostate that differ from those elicited by testosterone and estradiol
Article Snippet: .. Briefly, male experimental mice were housed for four h in clean cages lined with three mm Whatman filter paper (Fisher Scientific No. 057163W). ..

Electrophoresis:

Article Title: Cell cycle checkpoint status in human malignant mesothelioma cell lines: response to gamma radiation
Article Snippet: .. Each PCR product was mixed with one volume of denaturating solution (95% formamide, 20 mM EDTA, 0.05% xylene cyanol, 0.05% bromophenol blue), heated for 5 min at 95°C, cooled on ice and subjected to nondenaturing electrophoresis in a 10% PAGE (Clean gel 48S kit, Amersham Pharmacia Biotech, Sweden). ..

Incubation:

Article Title: THE USE OF MULTIPLE DISPLACEMENT AMPLIFICATION TO INCREASE THE DETECTION AND GENOTYPING OF TRYPANOSOMA SPECIES SAMPLES IMMOBILISED ON FTA FILTERS
Article Snippet: .. For use in PCR, discs were punched out of the FTA blood spot using a clean 2 mm diameter Harris Micro-punch™ (Whatman), the discs were washed 3 times with 200 μL FTA purification reagent (Whatman), and twice with 200 μL 1 mM TE (10 mM Tris, 1 mM EDTA) buffer pH 8.0, with incubation for 5 minutes at each wash as per the manufacturer’s instructions. .. The washes were necessary to reduce the many potential PCR inhibitors present in blood.

other:

Article Title: A1BG and C3 are overexpressed in patients with cervical intraepithelial neoplasia III
Article Snippet: Other interfering substances, including detergents, salts, lipids, nucleic acids and phenolic acids, were removed from the samples using the Two-dimensional (2D) Clean Up kit (GE Healthcare; Little Chalfont, UK).

Polyacrylamide Gel Electrophoresis:

Article Title: Cell cycle checkpoint status in human malignant mesothelioma cell lines: response to gamma radiation
Article Snippet: .. Each PCR product was mixed with one volume of denaturating solution (95% formamide, 20 mM EDTA, 0.05% xylene cyanol, 0.05% bromophenol blue), heated for 5 min at 95°C, cooled on ice and subjected to nondenaturing electrophoresis in a 10% PAGE (Clean gel 48S kit, Amersham Pharmacia Biotech, Sweden). ..

Article Title: Recurrent Soft Tissue Abscesses Caused by Legionella cincinnatiensis
Article Snippet: .. This fragment was purified by horizontal polyacrylamide gel electrophoresis (Clean Gel 365; Amersham Pharmacia Dübendorf, Switzerland). .. After staining with silver, excision of the band was followed by reamplification with primers BAK-11w and BAK-533r.

Polymerase Chain Reaction:

Article Title: THE USE OF MULTIPLE DISPLACEMENT AMPLIFICATION TO INCREASE THE DETECTION AND GENOTYPING OF TRYPANOSOMA SPECIES SAMPLES IMMOBILISED ON FTA FILTERS
Article Snippet: .. For use in PCR, discs were punched out of the FTA blood spot using a clean 2 mm diameter Harris Micro-punch™ (Whatman), the discs were washed 3 times with 200 μL FTA purification reagent (Whatman), and twice with 200 μL 1 mM TE (10 mM Tris, 1 mM EDTA) buffer pH 8.0, with incubation for 5 minutes at each wash as per the manufacturer’s instructions. .. The washes were necessary to reduce the many potential PCR inhibitors present in blood.

Article Title: DNA barcoding of the fire ant genus Solenopsis Westwood (Hymenoptera: Formicidae) from the Riyadh region, the Kingdom of Saudi Arabia
Article Snippet: .. In case of a single discrete amplified band, the PCR products were directly purified with FavorPrep™ MicroElute PCR clean up kit (GE Healthcare, Buckinghamshire, UK), whereas, in case of double bands, the required band was excised from the gel and purified with the same clean up kit. ..

Article Title: Cell cycle checkpoint status in human malignant mesothelioma cell lines: response to gamma radiation
Article Snippet: .. Each PCR product was mixed with one volume of denaturating solution (95% formamide, 20 mM EDTA, 0.05% xylene cyanol, 0.05% bromophenol blue), heated for 5 min at 95°C, cooled on ice and subjected to nondenaturing electrophoresis in a 10% PAGE (Clean gel 48S kit, Amersham Pharmacia Biotech, Sweden). ..

Lysis:

Article Title: Themis2/ICB1 Is a Signaling Scaffold That Selectively Regulates Macrophage Toll-Like Receptor Signaling and Cytokine Production
Article Snippet: .. Beads were washed (x5) in lysis buffer and bound proteins eluted by boiling in 2X SDS PAGE sample buffer or, for proteomic experiments, with FLAG peptide (1 mM, o/n) and concentrated using a 1D SDS PAGE clean up kit (GE Healthcare, Chalfont St. Giles, UK). .. Statistics Where appropriate, differences between datasets were analysed using a two-tailed paired Students T test.

Purification:

Article Title: THE USE OF MULTIPLE DISPLACEMENT AMPLIFICATION TO INCREASE THE DETECTION AND GENOTYPING OF TRYPANOSOMA SPECIES SAMPLES IMMOBILISED ON FTA FILTERS
Article Snippet: .. For use in PCR, discs were punched out of the FTA blood spot using a clean 2 mm diameter Harris Micro-punch™ (Whatman), the discs were washed 3 times with 200 μL FTA purification reagent (Whatman), and twice with 200 μL 1 mM TE (10 mM Tris, 1 mM EDTA) buffer pH 8.0, with incubation for 5 minutes at each wash as per the manufacturer’s instructions. .. The washes were necessary to reduce the many potential PCR inhibitors present in blood.

Article Title: DNA barcoding of the fire ant genus Solenopsis Westwood (Hymenoptera: Formicidae) from the Riyadh region, the Kingdom of Saudi Arabia
Article Snippet: .. In case of a single discrete amplified band, the PCR products were directly purified with FavorPrep™ MicroElute PCR clean up kit (GE Healthcare, Buckinghamshire, UK), whereas, in case of double bands, the required band was excised from the gel and purified with the same clean up kit. ..

Article Title: Recurrent Soft Tissue Abscesses Caused by Legionella cincinnatiensis
Article Snippet: .. This fragment was purified by horizontal polyacrylamide gel electrophoresis (Clean Gel 365; Amersham Pharmacia Dübendorf, Switzerland). .. After staining with silver, excision of the band was followed by reamplification with primers BAK-11w and BAK-533r.

Electrofocusing:

Article Title: Proteomic Analysis Reveals the Participation of Energy- and Stress-Related Proteins in the Response of Pseudomonas putida DOT-T1E to Toluene
Article Snippet: .. Extracted proteins were precipitated using the Ettan 2-D clean up kit (Amersham Biosciences) following the manufacturer's instructions and were subsequently resuspended in isoelectric focusing (IEF) buffer consisting of 7 M urea, 2 M thiourea, and 4% (wt/vol) 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS). .. For the first dimension of protein separation, IEF was performed using 24-cm immobilized nonlinear pH gradient strips (pH 3 to 10; GE Healthcare, Little Chalfont, United Kingdom).

SDS Page:

Article Title: Themis2/ICB1 Is a Signaling Scaffold That Selectively Regulates Macrophage Toll-Like Receptor Signaling and Cytokine Production
Article Snippet: .. Beads were washed (x5) in lysis buffer and bound proteins eluted by boiling in 2X SDS PAGE sample buffer or, for proteomic experiments, with FLAG peptide (1 mM, o/n) and concentrated using a 1D SDS PAGE clean up kit (GE Healthcare, Chalfont St. Giles, UK). .. Statistics Where appropriate, differences between datasets were analysed using a two-tailed paired Students T test.

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    GE Healthcare exosap it pcr clean up reagent
    Exosap It Pcr Clean Up Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exosap it pcr clean up reagent/product/GE Healthcare
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    exosap it pcr clean up reagent - by Bioz Stars, 2020-09
    94/100 stars
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