exonuclease i (GE Healthcare)
91
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GE Healthcare
exonuclease i
Exonuclease I, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/exonuclease i/product/GE Healthcare
Average 91 stars, based on 54 article reviews
Price from $9.99 to $1999.99
Exonuclease I, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/exonuclease i/product/GE Healthcare
Average 91 stars, based on 54 article reviews
Price from $9.99 to $1999.99
exonuclease i - by Bioz Stars,
2019-12
91/100 stars
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Clone Assay:Article Title: Endogenous TCR Recombination in TCR Tg Single RAG-Deficient Mice Uncovered by Robust In Vivo T Cell Activation and Selection Article Snippet: PCR products were then incubated with 0.5 U of shrimp alkaline phosphatase and 5 U of Amplification:Article Title: The human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation Article Snippet: Incubation conditions were as follows: 15 min at 50°C for RT, 2 min at 95°C for Taq inactivation, followed by a touch-down PCR amplification with 20 cycles of 15 s at 95°C for denaturation and 4 min for annealing and extension (decreasing temperature from 70°C down to 60°C over 5 cycles, followed by 15 cycles at 60°C) . .. Amplification was followed by DNA digestion with Article Title: DNA damage predicts prognosis and treatment response in colorectal liver metastases superior to immunogenic cell death and T cells Article Snippet: Briefly, genomic DNA was extracted from tissue blocks and exon 2 and 3 of the KRAS gene and exon 15 of the BRAF gene were polymerase chain reaction (PCR) amplified with AmpliTaq Gold® DNA polymerase (Applied Biosystems, Fisher Scientific, Vienna, Austria) and corresponding oligonucleotide primers ( Table ). .. Excess of primers and deoxynucleotides (dNTPs) was removed by incubation of 5 µL PCR product with 2.5 U Article Title: Ancient female philopatry, asymmetric male gene flow, and synchronous population expansion support the influence of climatic oscillations on the evolution of South American sea lion (Otaria flavescens) Article Snippet: Amplifications for CR and cytb were carried out in 20 μl with the following conditions: 1.5 mM MgCl2, 200 μM of each dNTP, 0.1 μM of each primer, 1 U of Platinum Taq DNA polymerase (Invitrogen), 1X PCR buffer (Invitrogen), 0.2%–0.4% Triton and 2 μl of DNA (approximately 50 ng). .. Amplification products were purified with shrimp alkaline phosphatase and Article Title: Complete mitochondrial DNA sequence of the endangered fish (Bahabataipingensis): Mitogenome characterization and phylogenetic implications Article Snippet: Paragraph title: Mitochondrial DNA amplification ... PCR products were cleaned by adding 0.45 µl of Shrimp Alkaline Phosphatase (Biotech Pharmacon), 0.9 µl of Article Title: Consuming viscous prey: a novel protein-secreting delivery system in neotropical snail-eating snakes Article Snippet: Primers and PCR protocols for partial amplification of genes 12S, 16S, cytb, bdnf, and c-mos were those described in Grazziotin et al. [ ]. .. PCRs were purified with shrimp alkaline phosphatase and Article Title: Behavioural and Genetic Characteristics of a New Species of Nasonia Article Snippet: NCBI accession numbers for each fragment from all the strains are summarized in . .. To clean the reactions before sequencing, amplified reactions (8uL) were incubated with 0.5 U shrimp alkaline phosphatase and 1.0 U of Article Title: Specific variants in WDR35 cause a distinctive form of Ellis-van Creveld syndrome by disrupting the recruitment of the EvC complex and SMO into the cilium Article Snippet: WDR 35 coding exons and at least 100 bp of the adjacent introns were amplified by standard PCR from peripheral blood genomic DNA. .. PCR products were enzymatically treated with alkaline phosphatase and Article Title: Identification of a Mutation Causing Deficient BMP1/mTLD Proteolytic Activity in Autosomal Recessive Osteogenesis Imperfecta Article Snippet: BMP1 coding exons and at least 100 bp of the flanking introns were amplified by standard PCR (primers available on request). .. Prior to sequencing, the PCR products were treated with shrimp alkaline phosphatase and Article Title: FGFR3 protein expression and its relationship to mutation status and prognostic variables in bladder cancer Article Snippet: 2 μl samples of extracted DNA were amplified in 50 μl reactions using AmpliTaq Gold (Applied Biosystems, Foster City, CA, USA). .. For sequencing, unincorporated primers and deoxynucleotides were removed using shrimp alkaline phosphatase and Article Title: Evolution of Smooth Tubercle Bacilli PE and PE_PGRS Genes: Evidence for a Prominent Role of Recombination and Imprint of Positive Selection Article Snippet: Paragraph title: PCR amplification and DNA sequencing ... Amplicons were subjected to sequencing after treatment with Article Title: Association of Mitochondrial DNA Polymerase ? Gene POLG1 Polymorphisms with Parkinsonism in Chinese Populations Article Snippet: PCR conditions were set as follows: 2 min initial denaturation at 95°C, 30 cycles of 30 s denaturation at 94°C, 30 s annealing at 53–60°C, 30 s extension at 72°C and 10 min final extension at 72°C. .. Before sequencing, amplification products were incubated with shrimp alkaline phosphatase (0.5 units; Amersham) and Article Title: Endogenous TCR Recombination in TCR Tg Single RAG-Deficient Mice Uncovered by Robust In Vivo T Cell Activation and Selection Article Snippet: PCR products were then incubated with 0.5 U of shrimp alkaline phosphatase and 5 U of Article Title: Population Genetic Structure of the Magnificent Frigatebird Fregata magnificens (Aves, Suliformes) Breeding Colonies in the Western Atlantic Ocean Article Snippet: The PCR conditions were the same for the two mitochondrial segments: 10 cycles of 94°C for 60 s, 60°C for 60 s (-1μC/cycle), and 72°C for 60 s, followed by 35 cycles of 94°C for 60 s, 55°C for 60 s, and 72°C for 60 s, and a final extension of 72°C for 10 min. .. The amplified products were assessed on a 1% agarose gel, purified enzymatically with shrimp alkaline phosphatase and Article Title: A Family of H723R Mutation for SLC26A4 Associated with Enlarged Vestibular Aqueduct Syndrome Article Snippet: Amplification was carried out in a Perkin-Elmer thermal cycler using the following conditions: 5-min denaturation at 95℃, 37 three-step cycles (95℃ for 30 sec, 55℃ for 1 min, 72℃ for 1 or 3 min), 72℃ for 10 min, and ending with a holding period at 4℃. .. The PCR products were directly sequenced after removal of unincorporated dNTPs and primers by incubation of 50-100 ng PCR product at 37℃ for 30 min with 0.1 µL Polymerase Chain Reaction:Article Title: The human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation Article Snippet: Incubation conditions were as follows: 15 min at 50°C for RT, 2 min at 95°C for Taq inactivation, followed by a touch-down PCR amplification with 20 cycles of 15 s at 95°C for denaturation and 4 min for annealing and extension (decreasing temperature from 70°C down to 60°C over 5 cycles, followed by 15 cycles at 60°C) . .. Amplification was followed by DNA digestion with Article Title: DNA damage predicts prognosis and treatment response in colorectal liver metastases superior to immunogenic cell death and T cells Article Snippet: Briefly, genomic DNA was extracted from tissue blocks and exon 2 and 3 of the KRAS gene and exon 15 of the BRAF gene were polymerase chain reaction (PCR) amplified with AmpliTaq Gold® DNA polymerase (Applied Biosystems, Fisher Scientific, Vienna, Austria) and corresponding oligonucleotide primers ( Table ). .. Excess of primers and deoxynucleotides (dNTPs) was removed by incubation of 5 µL PCR product with 2.5 U Article Title: Ancient female philopatry, asymmetric male gene flow, and synchronous population expansion support the influence of climatic oscillations on the evolution of South American sea lion (Otaria flavescens) Article Snippet: Amplifications for CR and cytb were carried out in 20 μl with the following conditions: 1.5 mM MgCl2, 200 μM of each dNTP, 0.1 μM of each primer, 1 U of Platinum Taq DNA polymerase (Invitrogen), 1X PCR buffer (Invitrogen), 0.2%–0.4% Triton and 2 μl of DNA (approximately 50 ng). .. Amplification products were purified with shrimp alkaline phosphatase and Article Title: Colorectal Tumors from APC*I1307K Carriers Principally Harbor Somatic APC Mutations outside the A8 Tract Article Snippet: Samples in which a variant was identified by FSSCP were re-amplified using unlabeled PCR primers. .. PCR products were prepared for sequencing by treatment of 5 µl of PCR product with 2 U of shrimp alkaline phosphatase and 10 U of Article Title: Complete mitochondrial DNA sequence of the endangered fish (Bahabataipingensis): Mitogenome characterization and phylogenetic implications Article Snippet: Negative controls were included in all PCR amplifications to confirm the absence of contaminants. .. PCR products were cleaned by adding 0.45 µl of Shrimp Alkaline Phosphatase (Biotech Pharmacon), 0.9 µl of Article Title: Consuming viscous prey: a novel protein-secreting delivery system in neotropical snail-eating snakes Article Snippet: Primers and PCR protocols for partial amplification of genes 12S, 16S, cytb, bdnf, and c-mos were those described in Grazziotin et al. [ ]. .. PCRs were purified with shrimp alkaline phosphatase and Article Title: Specific variants in WDR35 cause a distinctive form of Ellis-van Creveld syndrome by disrupting the recruitment of the EvC complex and SMO into the cilium Article Snippet: WDR 35 coding exons and at least 100 bp of the adjacent introns were amplified by standard PCR from peripheral blood genomic DNA. .. PCR products were enzymatically treated with alkaline phosphatase and Article Title: Identification of a Mutation Causing Deficient BMP1/mTLD Proteolytic Activity in Autosomal Recessive Osteogenesis Imperfecta Article Snippet: BMP1 coding exons and at least 100 bp of the flanking introns were amplified by standard PCR (primers available on request). .. Prior to sequencing, the PCR products were treated with shrimp alkaline phosphatase and Article Title: FGFR3 protein expression and its relationship to mutation status and prognostic variables in bladder cancer Article Snippet: Primers used for PCR amplification have been described previously [ ]. .. For sequencing, unincorporated primers and deoxynucleotides were removed using shrimp alkaline phosphatase and Article Title: Evolution of Smooth Tubercle Bacilli PE and PE_PGRS Genes: Evidence for a Prominent Role of Recombination and Imprint of Positive Selection Article Snippet: Paragraph title: PCR amplification and DNA sequencing ... Amplicons were subjected to sequencing after treatment with Article Title: Genetic Analyses of Heme Oxygenase 1 (HMOX1) in Different Forms of Pancreatitis Article Snippet: Oligonucleotide sequences and annealing temperatures of the primers are listed in . .. We digested PCR products with shrimp alkaline phosphatase (USB) and Article Title: Association of Mitochondrial DNA Polymerase ? Gene POLG1 Polymorphisms with Parkinsonism in Chinese Populations Article Snippet: Paragraph title: PCR and SNP screening by sequencing ... Before sequencing, amplification products were incubated with shrimp alkaline phosphatase (0.5 units; Amersham) and Article Title: Endogenous TCR Recombination in TCR Tg Single RAG-Deficient Mice Uncovered by Robust In Vivo T Cell Activation and Selection Article Snippet: For single peak Vα and Vβ profiles, direct sequencing was performed with AV and AC, and BV and BC primers following the recommendations of the BigDye Terminator v1.1 Cycle Sequencing Ready Reaction kit (Applied Biosystems). .. PCR products were then incubated with 0.5 U of shrimp alkaline phosphatase and 5 U of Article Title: Population Genetic Structure of the Magnificent Frigatebird Fregata magnificens (Aves, Suliformes) Breeding Colonies in the Western Atlantic Ocean Article Snippet: The amplified products were assessed on a 1% agarose gel, purified enzymatically with shrimp alkaline phosphatase and exonuclease I (GE Healthcare), and Sanger sequenced by Macrogen Inc., South Korea. .. The amplified products were assessed on a 1% agarose gel, purified enzymatically with shrimp alkaline phosphatase and Article Title: A Family of H723R Mutation for SLC26A4 Associated with Enlarged Vestibular Aqueduct Syndrome Article Snippet: Amplification was carried out in a Perkin-Elmer thermal cycler using the following conditions: 5-min denaturation at 95℃, 37 three-step cycles (95℃ for 30 sec, 55℃ for 1 min, 72℃ for 1 or 3 min), 72℃ for 10 min, and ending with a holding period at 4℃. .. The PCR products were directly sequenced after removal of unincorporated dNTPs and primers by incubation of 50-100 ng PCR product at 37℃ for 30 min with 0.1 µL Article Title: Ghrelin Gene Variants Influence on Metabolic Syndrome Components in Aged Spanish Population Article Snippet: Products were treated with Exonuclease I (Amersham Biosciences) and shrimp alkaline phosphatase (Amersham Biosciences) to remove excess primers and deoxynucleotide triphosphates. .. Products were treated with Article Title: Mutation Spectrum of Meckel Syndrome Genes: One Group of Syndromes or Several Distinct Groups? Article Snippet: Primer sequences are available upon request. .. The PCR reactions were done with AmpliTaq Gold and PCR products were treated with shrimp alkaline phosphatase (SAP) and TA Cloning:Article Title: Endogenous TCR Recombination in TCR Tg Single RAG-Deficient Mice Uncovered by Robust In Vivo T Cell Activation and Selection Article Snippet: PCR products were then incubated with 0.5 U of shrimp alkaline phosphatase and 5 U of Construct:Article Title: Behavioural and Genetic Characteristics of a New Species of Nasonia Article Snippet: To clean the reactions before sequencing, amplified reactions (8uL) were incubated with 0.5 U shrimp alkaline phosphatase and 1.0 U of SYBR Green Assay:Article Title: The human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation Article Snippet: Incubation conditions were as follows: 15 min at 50°C for RT, 2 min at 95°C for Taq inactivation, followed by a touch-down PCR amplification with 20 cycles of 15 s at 95°C for denaturation and 4 min for annealing and extension (decreasing temperature from 70°C down to 60°C over 5 cycles, followed by 15 cycles at 60°C) . .. Amplification was followed by DNA digestion with Incubation:Article Title: The human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation Article Snippet: Incubation conditions were as follows: 15 min at 50°C for RT, 2 min at 95°C for Taq inactivation, followed by a touch-down PCR amplification with 20 cycles of 15 s at 95°C for denaturation and 4 min for annealing and extension (decreasing temperature from 70°C down to 60°C over 5 cycles, followed by 15 cycles at 60°C) . .. Amplification was followed by DNA digestion with Article Title: DNA damage predicts prognosis and treatment response in colorectal liver metastases superior to immunogenic cell death and T cells Article Snippet: Briefly, genomic DNA was extracted from tissue blocks and exon 2 and 3 of the KRAS gene and exon 15 of the BRAF gene were polymerase chain reaction (PCR) amplified with AmpliTaq Gold® DNA polymerase (Applied Biosystems, Fisher Scientific, Vienna, Austria) and corresponding oligonucleotide primers ( Table ). .. Excess of primers and deoxynucleotides (dNTPs) was removed by incubation of 5 µL PCR product with 2.5 U Article Title: Nanoliter high throughput quantitative PCR Article Snippet: Cultures were incubated for 4 h at 37C, 5% CO2 . .. Samples were treated with Article Title: Behavioural and Genetic Characteristics of a New Species of Nasonia Article Snippet: NCBI accession numbers for each fragment from all the strains are summarized in . .. To clean the reactions before sequencing, amplified reactions (8uL) were incubated with 0.5 U shrimp alkaline phosphatase and 1.0 U of Article Title: Association of Mitochondrial DNA Polymerase ? Gene POLG1 Polymorphisms with Parkinsonism in Chinese Populations Article Snippet: PCR conditions were set as follows: 2 min initial denaturation at 95°C, 30 cycles of 30 s denaturation at 94°C, 30 s annealing at 53–60°C, 30 s extension at 72°C and 10 min final extension at 72°C. .. Before sequencing, amplification products were incubated with shrimp alkaline phosphatase (0.5 units; Amersham) and Article Title: Endogenous TCR Recombination in TCR Tg Single RAG-Deficient Mice Uncovered by Robust In Vivo T Cell Activation and Selection Article Snippet: For single peak Vα and Vβ profiles, direct sequencing was performed with AV and AC, and BV and BC primers following the recommendations of the BigDye Terminator v1.1 Cycle Sequencing Ready Reaction kit (Applied Biosystems). .. PCR products were then incubated with 0.5 U of shrimp alkaline phosphatase and 5 U of Article Title: A Family of H723R Mutation for SLC26A4 Associated with Enlarged Vestibular Aqueduct Syndrome Article Snippet: Amplification was carried out in a Perkin-Elmer thermal cycler using the following conditions: 5-min denaturation at 95℃, 37 three-step cycles (95℃ for 30 sec, 55℃ for 1 min, 72℃ for 1 or 3 min), 72℃ for 10 min, and ending with a holding period at 4℃. .. The PCR products were directly sequenced after removal of unincorporated dNTPs and primers by incubation of 50-100 ng PCR product at 37℃ for 30 min with 0.1 µL Expressing:Article Title: The human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation Article Snippet: Amplification was followed by DNA digestion with Countercurrent Chromatography:Article Title: Ancient female philopatry, asymmetric male gene flow, and synchronous population expansion support the influence of climatic oscillations on the evolution of South American sea lion (Otaria flavescens) Article Snippet: The mitochondrial DNA control region (CR) and cytochrome b (cytb) gene were partially amplified by PCR using the following primers: L15926 5´- TCA AAG CTT ACA CCA GTC TTG TAA ACC—3´ [ ]; H16498 5´- CCT GAA GTA GGA ACC AGA TG—3´ [ ] for the control region and GLUDG-L 5′-TGA CTT GAA RAA CCA YCG TTG-3′ and CB2-H 5′- CCC TCA GAA TGA TAT TTG TCC TCA-3′ [ ] for the cytb. .. Amplification products were purified with shrimp alkaline phosphatase and Flow Cytometry:Article Title: The human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation Article Snippet: Briefly, for each reaction 100 flow-sorted cells were incubated with 0.2 µL SuperScript III RT Platinum Taq Mix and 2.5 µL of primer mix in a final volume of 9 µL. .. Amplification was followed by DNA digestion with Gas Chromatography:Article Title: Evolution of Smooth Tubercle Bacilli PE and PE_PGRS Genes: Evidence for a Prominent Role of Recombination and Imprint of Positive Selection Article Snippet: Although the genome sequence of some reference strains is publicly available, we PCR amplified and sequenced the PE/PE_PGRS selected members from the DNA of all these strains, since the accuracy of genome sequences in these highly GC-rich and repetitive regions is questioned. .. Amplicons were subjected to sequencing after treatment with Genomic Sequencing:Article Title: Identification of a Mutation Causing Deficient BMP1/mTLD Proteolytic Activity in Autosomal Recessive Osteogenesis Imperfecta Article Snippet: Paragraph title: Genomic Sequencing ... Prior to sequencing, the PCR products were treated with shrimp alkaline phosphatase and Generated:Article Title: Behavioural and Genetic Characteristics of a New Species of Nasonia Article Snippet: To clean the reactions before sequencing, amplified reactions (8uL) were incubated with 0.5 U shrimp alkaline phosphatase and 1.0 U of DNA Sequencing:Article Title: Colorectal Tumors from APC*I1307K Carriers Principally Harbor Somatic APC Mutations outside the A8 Tract Article Snippet: Paragraph title: Extended DNA Sequencing ... PCR products were prepared for sequencing by treatment of 5 µl of PCR product with 2 U of shrimp alkaline phosphatase and 10 U of Article Title: Evolution of Smooth Tubercle Bacilli PE and PE_PGRS Genes: Evidence for a Prominent Role of Recombination and Imprint of Positive Selection Article Snippet: Paragraph title: PCR amplification and DNA sequencing ... Amplicons were subjected to sequencing after treatment with Article Title: Genetic Analyses of Heme Oxygenase 1 (HMOX1) in Different Forms of Pancreatitis Article Snippet: Paragraph title: Polymerase Chain Reaction and DNA sequencing ... We digested PCR products with shrimp alkaline phosphatase (USB) and Reverse Transcription Polymerase Chain Reaction:Article Title: The human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation Article Snippet: Reverse transcription (RT) and cDNA amplification were carried out in one step using the SuperScript III One-Step RT-PCR System (Invitrogen). .. Amplification was followed by DNA digestion with Recombinant:Article Title: Nanoliter high throughput quantitative PCR Article Snippet: Recombinant Human TNF-α (10 ng/ml in EGM™-2), purchased from R & D Systems Inc. (Minneapolis, MN) was added to HUVEC cultures that were ∼50% confluent in T150 tissue culture flasks. .. Samples were treated with DNA Extraction:Article Title: FGFR3 protein expression and its relationship to mutation status and prognostic variables in bladder cancer Article Snippet: Paragraph title: DNA extraction and FGFR3 mutation analysis ... For sequencing, unincorporated primers and deoxynucleotides were removed using shrimp alkaline phosphatase and Mutagenesis:Article Title: DNA damage predicts prognosis and treatment response in colorectal liver metastases superior to immunogenic cell death and T cells Article Snippet: Paragraph title: Analysis of mutation status ... Excess of primers and deoxynucleotides (dNTPs) was removed by incubation of 5 µL PCR product with 2.5 U Article Title: Identification of a Mutation Causing Deficient BMP1/mTLD Proteolytic Activity in Autosomal Recessive Osteogenesis Imperfecta Article Snippet: Prior to sequencing, the PCR products were treated with shrimp alkaline phosphatase and Article Title: FGFR3 protein expression and its relationship to mutation status and prognostic variables in bladder cancer Article Snippet: Paragraph title: DNA extraction and FGFR3 mutation analysis ... For sequencing, unincorporated primers and deoxynucleotides were removed using shrimp alkaline phosphatase and Article Title: Genetic Analyses of Heme Oxygenase 1 (HMOX1) in Different Forms of Pancreatitis Article Snippet: We digested PCR products with shrimp alkaline phosphatase (USB) and Article Title: Mutation Spectrum of Meckel Syndrome Genes: One Group of Syndromes or Several Distinct Groups? Article Snippet: Paragraph title: Mutation Analysis ... The PCR reactions were done with AmpliTaq Gold and PCR products were treated with shrimp alkaline phosphatase (SAP) and Isolation:Article Title: Ghrelin Gene Variants Influence on Metabolic Syndrome Components in Aged Spanish Population Article Snippet: DNA was isolated from peripheral blood cells using the Chemagic System (Chemagen; Baesweiler, Germany). .. Products were treated with Size-exclusion Chromatography:Article Title: A Family of H723R Mutation for SLC26A4 Associated with Enlarged Vestibular Aqueduct Syndrome Article Snippet: Amplification was carried out in a Perkin-Elmer thermal cycler using the following conditions: 5-min denaturation at 95℃, 37 three-step cycles (95℃ for 30 sec, 55℃ for 1 min, 72℃ for 1 or 3 min), 72℃ for 10 min, and ending with a holding period at 4℃. .. The PCR products were directly sequenced after removal of unincorporated dNTPs and primers by incubation of 50-100 ng PCR product at 37℃ for 30 min with 0.1 µL Purification:Article Title: DNA damage predicts prognosis and treatment response in colorectal liver metastases superior to immunogenic cell death and T cells Article Snippet: Briefly, genomic DNA was extracted from tissue blocks and exon 2 and 3 of the KRAS gene and exon 15 of the BRAF gene were polymerase chain reaction (PCR) amplified with AmpliTaq Gold® DNA polymerase (Applied Biosystems, Fisher Scientific, Vienna, Austria) and corresponding oligonucleotide primers ( Table ). .. Excess of primers and deoxynucleotides (dNTPs) was removed by incubation of 5 µL PCR product with 2.5 U Article Title: Ancient female philopatry, asymmetric male gene flow, and synchronous population expansion support the influence of climatic oscillations on the evolution of South American sea lion (Otaria flavescens) Article Snippet: Amplifications for CR and cytb were carried out in 20 μl with the following conditions: 1.5 mM MgCl2, 200 μM of each dNTP, 0.1 μM of each primer, 1 U of Platinum Taq DNA polymerase (Invitrogen), 1X PCR buffer (Invitrogen), 0.2%–0.4% Triton and 2 μl of DNA (approximately 50 ng). .. Amplification products were purified with shrimp alkaline phosphatase and Article Title: Nanoliter high throughput quantitative PCR Article Snippet: RNA was purified using an RNeasy Mini Kit (Qiagen Inc.) and reverse transcription was done using a High Capacity cDNA Archive Kit (Applied Biosystems). .. Samples were treated with Article Title: Consuming viscous prey: a novel protein-secreting delivery system in neotropical snail-eating snakes Article Snippet: We used the primers and protocols described in Vidal and Hedges [ ], Noonan and Chippindale [ ], and Chiari et al. [ ] to amplify fragments for the nuclear genes jun, nt3, and rag1, respectively. .. PCRs were purified with shrimp alkaline phosphatase and Article Title: Population Genetic Structure of the Magnificent Frigatebird Fregata magnificens (Aves, Suliformes) Breeding Colonies in the Western Atlantic Ocean Article Snippet: The PCR conditions were the same for the two mitochondrial segments: 10 cycles of 94°C for 60 s, 60°C for 60 s (-1μC/cycle), and 72°C for 60 s, followed by 35 cycles of 94°C for 60 s, 55°C for 60 s, and 72°C for 60 s, and a final extension of 72°C for 10 min. .. The amplified products were assessed on a 1% agarose gel, purified enzymatically with shrimp alkaline phosphatase and Article Title: Ghrelin Gene Variants Influence on Metabolic Syndrome Components in Aged Spanish Population Article Snippet: Products were treated with Sequencing:Article Title: DNA damage predicts prognosis and treatment response in colorectal liver metastases superior to immunogenic cell death and T cells Article Snippet: Briefly, genomic DNA was extracted from tissue blocks and exon 2 and 3 of the KRAS gene and exon 15 of the BRAF gene were polymerase chain reaction (PCR) amplified with AmpliTaq Gold® DNA polymerase (Applied Biosystems, Fisher Scientific, Vienna, Austria) and corresponding oligonucleotide primers ( Table ). .. Excess of primers and deoxynucleotides (dNTPs) was removed by incubation of 5 µL PCR product with 2.5 U Article Title: Ancient female philopatry, asymmetric male gene flow, and synchronous population expansion support the influence of climatic oscillations on the evolution of South American sea lion (Otaria flavescens) Article Snippet: Paragraph title: Mitochondrial DNA amplification, sequencing and analysis ... Amplification products were purified with shrimp alkaline phosphatase and Article Title: Colorectal Tumors from APC*I1307K Carriers Principally Harbor Somatic APC Mutations outside the A8 Tract Article Snippet: Samples in which a variant was identified by FSSCP were re-amplified using unlabeled PCR primers. .. PCR products were prepared for sequencing by treatment of 5 µl of PCR product with 2 U of shrimp alkaline phosphatase and 10 U of Article Title: Consuming viscous prey: a novel protein-secreting delivery system in neotropical snail-eating snakes Article Snippet: We used the primers and protocols described in Vidal and Hedges [ ], Noonan and Chippindale [ ], and Chiari et al. [ ] to amplify fragments for the nuclear genes jun, nt3, and rag1, respectively. .. PCRs were purified with shrimp alkaline phosphatase and Article Title: Behavioural and Genetic Characteristics of a New Species of Nasonia Article Snippet: NCBI accession numbers for each fragment from all the strains are summarized in . .. To clean the reactions before sequencing, amplified reactions (8uL) were incubated with 0.5 U shrimp alkaline phosphatase and 1.0 U of Article Title: Specific variants in WDR35 cause a distinctive form of Ellis-van Creveld syndrome by disrupting the recruitment of the EvC complex and SMO into the cilium Article Snippet: WDR 35 coding exons and at least 100 bp of the adjacent introns were amplified by standard PCR from peripheral blood genomic DNA. .. PCR products were enzymatically treated with alkaline phosphatase and Article Title: Identification of a Mutation Causing Deficient BMP1/mTLD Proteolytic Activity in Autosomal Recessive Osteogenesis Imperfecta Article Snippet: BMP1 coding exons and at least 100 bp of the flanking introns were amplified by standard PCR (primers available on request). .. Prior to sequencing, the PCR products were treated with shrimp alkaline phosphatase and Article Title: FGFR3 protein expression and its relationship to mutation status and prognostic variables in bladder cancer Article Snippet: 2 μl samples of extracted DNA were amplified in 50 μl reactions using AmpliTaq Gold (Applied Biosystems, Foster City, CA, USA). .. For sequencing, unincorporated primers and deoxynucleotides were removed using shrimp alkaline phosphatase and Article Title: Evolution of Smooth Tubercle Bacilli PE and PE_PGRS Genes: Evidence for a Prominent Role of Recombination and Imprint of Positive Selection Article Snippet: The amplification ended with a final elongation step of 7 min at 72°C. .. Amplicons were subjected to sequencing after treatment with Article Title: Genetic Analyses of Heme Oxygenase 1 (HMOX1) in Different Forms of Pancreatitis Article Snippet: Oligonucleotide sequences and annealing temperatures of the primers are listed in . .. We digested PCR products with shrimp alkaline phosphatase (USB) and Article Title: Association of Mitochondrial DNA Polymerase ? Gene POLG1 Polymorphisms with Parkinsonism in Chinese Populations Article Snippet: PCR conditions were set as follows: 2 min initial denaturation at 95°C, 30 cycles of 30 s denaturation at 94°C, 30 s annealing at 53–60°C, 30 s extension at 72°C and 10 min final extension at 72°C. .. Before sequencing, amplification products were incubated with shrimp alkaline phosphatase (0.5 units; Amersham) and Article Title: Endogenous TCR Recombination in TCR Tg Single RAG-Deficient Mice Uncovered by Robust In Vivo T Cell Activation and Selection Article Snippet: Paragraph title: Sequencing analyses ... PCR products were then incubated with 0.5 U of shrimp alkaline phosphatase and 5 U of Article Title: A Family of H723R Mutation for SLC26A4 Associated with Enlarged Vestibular Aqueduct Syndrome Article Snippet: Mutations were detected by a combination of intronic polymerase chain reaction (PCR) amplification, using primers flanking each exon, and sequencing of the amplification products. .. The PCR products were directly sequenced after removal of unincorporated dNTPs and primers by incubation of 50-100 ng PCR product at 37℃ for 30 min with 0.1 µL Article Title: Mutation Spectrum of Meckel Syndrome Genes: One Group of Syndromes or Several Distinct Groups? Article Snippet: Primer sequences are available upon request. .. The PCR reactions were done with AmpliTaq Gold and PCR products were treated with shrimp alkaline phosphatase (SAP) and Blocking Assay:Article Title: FGFR3 protein expression and its relationship to mutation status and prognostic variables in bladder cancer Article Snippet: For sequencing, unincorporated primers and deoxynucleotides were removed using shrimp alkaline phosphatase and exonuclease I (Amersham Biosciences, Chalfont, UK). .. For sequencing, unincorporated primers and deoxynucleotides were removed using shrimp alkaline phosphatase and Sample Prep:Article Title: Nanoliter high throughput quantitative PCR Article Snippet: Paragraph title: Human umbilical vein endothelial cells (HUVEC sample preparation ... Samples were treated with Plasmid Preparation:Article Title: Endogenous TCR Recombination in TCR Tg Single RAG-Deficient Mice Uncovered by Robust In Vivo T Cell Activation and Selection Article Snippet: PCR products were then incubated with 0.5 U of shrimp alkaline phosphatase and 5 U of Software:Article Title: The human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation Article Snippet: Amplification was followed by DNA digestion with Article Title: Specific variants in WDR35 cause a distinctive form of Ellis-van Creveld syndrome by disrupting the recruitment of the EvC complex and SMO into the cilium Article Snippet: PCR products were enzymatically treated with alkaline phosphatase and Article Title: FGFR3 protein expression and its relationship to mutation status and prognostic variables in bladder cancer Article Snippet: For sequencing, unincorporated primers and deoxynucleotides were removed using shrimp alkaline phosphatase and Article Title: Mutation Spectrum of Meckel Syndrome Genes: One Group of Syndromes or Several Distinct Groups? Article Snippet: Primers for amplifying the genomic DNA were designed using Primer3 software ( ) and provided by Sigma-Genosys. .. The PCR reactions were done with AmpliTaq Gold and PCR products were treated with shrimp alkaline phosphatase (SAP) and Real-time Polymerase Chain Reaction:Article Title: The human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation Article Snippet: Incubation conditions were as follows: 15 min at 50°C for RT, 2 min at 95°C for Taq inactivation, followed by a touch-down PCR amplification with 20 cycles of 15 s at 95°C for denaturation and 4 min for annealing and extension (decreasing temperature from 70°C down to 60°C over 5 cycles, followed by 15 cycles at 60°C) . .. Amplification was followed by DNA digestion with Article Title: Nanoliter high throughput quantitative PCR Article Snippet: RNA samples were converted to randomly primed first strand cDNA using High Capacity cDNA Archive Kit (Applied BioSystems, Foster City, CA). .. To reduce non-specific product formation during qPCR, the cDNA sample was heated to 75°C for 10 min to inactivate the reverse transcriptase; snap chilled on ice for 5 min, then treated 1 h with 1.3 U/μl Functional Assay:Article Title: Population Genetic Structure of the Magnificent Frigatebird Fregata magnificens (Aves, Suliformes) Breeding Colonies in the Western Atlantic Ocean Article Snippet: [ ] using species-specific primers, strongly suggesting that we are studying the functional cytb copy. .. The amplified products were assessed on a 1% agarose gel, purified enzymatically with shrimp alkaline phosphatase and Agarose Gel Electrophoresis:Article Title: Population Genetic Structure of the Magnificent Frigatebird Fregata magnificens (Aves, Suliformes) Breeding Colonies in the Western Atlantic Ocean Article Snippet: The PCR conditions were the same for the two mitochondrial segments: 10 cycles of 94°C for 60 s, 60°C for 60 s (-1μC/cycle), and 72°C for 60 s, followed by 35 cycles of 94°C for 60 s, 55°C for 60 s, and 72°C for 60 s, and a final extension of 72°C for 10 min. .. The amplified products were assessed on a 1% agarose gel, purified enzymatically with shrimp alkaline phosphatase and Electrophoresis:Article Title: Ghrelin Gene Variants Influence on Metabolic Syndrome Components in Aged Spanish Population Article Snippet: Products were treated with Exonuclease I (Amersham Biosciences) and shrimp alkaline phosphatase (Amersham Biosciences) to remove excess primers and deoxynucleotide triphosphates. .. Products were treated with Ethanol Precipitation:Article Title: Colorectal Tumors from APC*I1307K Carriers Principally Harbor Somatic APC Mutations outside the A8 Tract Article Snippet: PCR products were prepared for sequencing by treatment of 5 µl of PCR product with 2 U of shrimp alkaline phosphatase and 10 U of Sampling:Article Title: Consuming viscous prey: a novel protein-secreting delivery system in neotropical snail-eating snakes Article Snippet: Sixty-one Dipsadinae terminals composed the ingroup, representing an increase of 25 species in respect to the taxon sampling used by Pyron et al. [ ] and 31 species to the one used by Grazziotin et al. [ ]. .. PCRs were purified with shrimp alkaline phosphatase and Variant Assay:Article Title: Colorectal Tumors from APC*I1307K Carriers Principally Harbor Somatic APC Mutations outside the A8 Tract Article Snippet: Samples in which a variant was identified by FSSCP were re-amplified using unlabeled PCR primers. .. PCR products were prepared for sequencing by treatment of 5 µl of PCR product with 2 U of shrimp alkaline phosphatase and 10 U of Article Title: Specific variants in WDR35 cause a distinctive form of Ellis-van Creveld syndrome by disrupting the recruitment of the EvC complex and SMO into the cilium Article Snippet: Paragraph title: Sequence variant screening and nomenclature ... PCR products were enzymatically treated with alkaline phosphatase and |