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GE Healthcare exonuclease i shrimp alkaline phosphatase
Exonuclease I Shrimp Alkaline Phosphatase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/exonuclease i shrimp alkaline phosphatase/product/GE Healthcare
Average 87 stars, based on 7 article reviews
Price from $9.99 to $1999.99
exonuclease i shrimp alkaline phosphatase - by Bioz Stars, 2020-01
87/100 stars

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Negative Control:

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: A mixture of 25 μL solution containing 1×Taq polymerase buffer B (Promega), 3 mM MgCl2 , 0.3 mM each dNTP, 0.4 μM primer L14841, 0.4 μM primer H15149, 1 unit Taq polymerase (Promega) with conditions of pre-heating at 95°C for 5 min, 36 cycles of consecutive incubations at 95°C for 30 sec, 60°C for 30 sec, 72°C for 40 sec and 72°C for 5 min. A negative control was included for each batch of assay. .. The final PCR products of 359 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems).

Amplification:

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: The nested amplification was carried out in a second tube, with the reaction mix again totaling 20 μl and containing the same reagents as the first stage, except that the primers were now 1 μM primer ITS1F and 1 μM primer ITS2R4, and the target DNA was provided by adding 1 μl of the completed first-stage PCR reaction. .. They were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using a BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems).

Article Title: Genetic Variation in the Interleukin-28B Gene Is Associated with Spontaneous Clearance and Progression of Hepatitis C Virus in Moroccan Patients
Article Snippet: The PCR reaction was carried out in a total volume of 25µl, containing 20 ng of genomic DNA, with the following amplification protocol: pre-incubation at 50°C for 2 min and then 95°C for 10 min, followed by 40 cycles of denaturation at 95°C for 15 s, and annealing/extension at 60°C for 1 min. .. The resulting 367-bp PCR product was purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) and sequenced using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems, Foster City, CA, USA).

Article Title: Variability in the Precore and Core Promoter Regions of HBV Strains in Morocco: Characterization and Impact on Liver Disease Progression
Article Snippet: .. The PCR condition was: preheating at 94°C for 2 min, then 35 cycles of amplification (94°C for 15 s, 58°C for 20 s and 72°C for 35 s) and final extension at 72°C for 4 min. A PCR product of 345 base pairs (bp) was obtained and subsequently purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare). .. The purified products were bidirectionally sequenced using the BigDye® Terminator Version 3.1 kit (Applied Biosystems, Foster City, CA, USA) and analyzed using an ABI PRISM 3130 automated sequencer (Applied Biosystems, Foster City, CA, USA).

Article Title: Detection and molecular typing of Leishmania tropica from Phlebotomus sergenti and lesions of cutaneous leishmaniasis in an emerging focus of Morocco
Article Snippet: The products obtained were subsequently subjected to nested PCR amplification as follows: the reaction mixtures of 20 μL each contained the same reagents as the first-stage PCR, except that the primers were now primer ITS1F and primer ITS2R4, and the target DNA was 1 μL of the first-stage PCR reaction products. .. The final PCR products of 400 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems).

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: Amplified DNA products were confirmed with 1.2% agarose gel and visualized under ultraviolet (UV) light after staining with 2 mg/mL ethidium bromide. .. The final PCR products of 359 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems).

Agarose Gel Electrophoresis:

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: Amplified DNA products were confirmed with 1.2% agarose gel and visualized under ultraviolet (UV) light after staining with 2 mg/mL ethidium bromide. .. The final PCR products of 359 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems).

Isolation:

Article Title: Genetic Variation in the Interleukin-28B Gene Is Associated with Spontaneous Clearance and Progression of Hepatitis C Virus in Moroccan Patients
Article Snippet: Paragraph title: Isolation of Genomic DNA and SNPs Genotyping ... The resulting 367-bp PCR product was purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) and sequenced using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems, Foster City, CA, USA).

Article Title: Variability in the Precore and Core Promoter Regions of HBV Strains in Morocco: Characterization and Impact on Liver Disease Progression
Article Snippet: HBV-DNA extraction, amplification and direct sequencing of Pre-C and CP regions HBV-DNA was isolated from 140 µl serum by proteinase K digestion followed by extraction with phenol chloroform and then precipitation with ethanol. .. The PCR condition was: preheating at 94°C for 2 min, then 35 cycles of amplification (94°C for 15 s, 58°C for 20 s and 72°C for 35 s) and final extension at 72°C for 4 min. A PCR product of 345 base pairs (bp) was obtained and subsequently purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare).

Size-exclusion Chromatography:

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: A mixture of 25 μL solution containing 1×Taq polymerase buffer B (Promega), 3 mM MgCl2 , 0.3 mM each dNTP, 0.4 μM primer L14841, 0.4 μM primer H15149, 1 unit Taq polymerase (Promega) with conditions of pre-heating at 95°C for 5 min, 36 cycles of consecutive incubations at 95°C for 30 sec, 60°C for 30 sec, 72°C for 40 sec and 72°C for 5 min. A negative control was included for each batch of assay. .. The final PCR products of 359 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems).

Construct:

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: They were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using a BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. A phylogenetic tree was constructed by using the Neighbor-Joining method in agreement with the Kimura 2-parameter model with uniform rates for transitions and transversions.

Article Title: Detection and molecular typing of Leishmania tropica from Phlebotomus sergenti and lesions of cutaneous leishmaniasis in an emerging focus of Morocco
Article Snippet: The final PCR products of 400 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. A phylogenetic tree was constructed by using the Neighbor-Joining method in agreement with Kimura 2-parameter model with uniform rates for transitions and transversions.

Purification:

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: .. They were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using a BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. Sequencing data were analyzed using SeqScape v.2.5 software (Applied Biosystems).

Article Title: Genetic Variation in the Interleukin-28B Gene Is Associated with Spontaneous Clearance and Progression of Hepatitis C Virus in Moroccan Patients
Article Snippet: .. The resulting 367-bp PCR product was purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) and sequenced using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems, Foster City, CA, USA). .. Sequencing data were analyzed using SeqScape v2.5 software (Applied Biosystems, Foster City, CA, USA).

Article Title: Molecular identification of Leishmania infection in the most relevant sand fly species and in patient skin samples from a cutaneous leishmaniasis focus, in Morocco
Article Snippet: .. The obtained 350-bp ITS1-PCR products were purified using exonuclease I/shrimp alkaline phosphatase (GE Healthcare, US). .. The sequencing reaction was performed in both directions using BigDye Terminator version 3.1 (Applied Biosystems, Foster City, CA, USA).

Article Title: Molecular identification of Leishmania infection in the most relevant sand fly species and in patient skin samples from a cutaneous leishmaniasis focus, in Morocco
Article Snippet: .. Sequencing and phylogenetic analysis The obtained 350-bp ITS1-PCR products were purified using exonuclease I/shrimp alkaline phosphatase (GE Healthcare, US). .. The sequencing reaction was performed in both directions using BigDye Terminator version 3.1 (Applied Biosystems, Foster City, CA, USA).

Article Title: Detection and molecular typing of Leishmania tropica from Phlebotomus sergenti and lesions of cutaneous leishmaniasis in an emerging focus of Morocco
Article Snippet: .. The final PCR products of 400 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. Sequencing data were analyzed using SeqScape v.2.5 software (Applied Biosystems).

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: .. The final PCR products of 359 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. Sequencing data were analyzed using SeqScape v.2.5 software (Applied Biosystems).

Sequencing:

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: .. They were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using a BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. Sequencing data were analyzed using SeqScape v.2.5 software (Applied Biosystems).

Article Title: Genetic Variation in the Interleukin-28B Gene Is Associated with Spontaneous Clearance and Progression of Hepatitis C Virus in Moroccan Patients
Article Snippet: .. The resulting 367-bp PCR product was purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) and sequenced using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems, Foster City, CA, USA). .. Sequencing data were analyzed using SeqScape v2.5 software (Applied Biosystems, Foster City, CA, USA).

Article Title: Molecular identification of Leishmania infection in the most relevant sand fly species and in patient skin samples from a cutaneous leishmaniasis focus, in Morocco
Article Snippet: Paragraph title: Sequencing and phylogenetic analysis ... The obtained 350-bp ITS1-PCR products were purified using exonuclease I/shrimp alkaline phosphatase (GE Healthcare, US).

Article Title: Variability in the Precore and Core Promoter Regions of HBV Strains in Morocco: Characterization and Impact on Liver Disease Progression
Article Snippet: Paragraph title: HBV-DNA extraction, amplification and direct sequencing of Pre-C and CP regions ... The PCR condition was: preheating at 94°C for 2 min, then 35 cycles of amplification (94°C for 15 s, 58°C for 20 s and 72°C for 35 s) and final extension at 72°C for 4 min. A PCR product of 345 base pairs (bp) was obtained and subsequently purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare).

Article Title: Molecular identification of Leishmania infection in the most relevant sand fly species and in patient skin samples from a cutaneous leishmaniasis focus, in Morocco
Article Snippet: .. Sequencing and phylogenetic analysis The obtained 350-bp ITS1-PCR products were purified using exonuclease I/shrimp alkaline phosphatase (GE Healthcare, US). .. The sequencing reaction was performed in both directions using BigDye Terminator version 3.1 (Applied Biosystems, Foster City, CA, USA).

Article Title: Detection and molecular typing of Leishmania tropica from Phlebotomus sergenti and lesions of cutaneous leishmaniasis in an emerging focus of Morocco
Article Snippet: .. The final PCR products of 400 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. Sequencing data were analyzed using SeqScape v.2.5 software (Applied Biosystems).

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: .. The final PCR products of 359 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. Sequencing data were analyzed using SeqScape v.2.5 software (Applied Biosystems).

Polymerase Chain Reaction:

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: The final PCR products of 462 bp were directly sequenced to identify Leishmania species infecting individual sand flies. .. They were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using a BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems).

Article Title: Genetic Variation in the Interleukin-28B Gene Is Associated with Spontaneous Clearance and Progression of Hepatitis C Virus in Moroccan Patients
Article Snippet: .. The resulting 367-bp PCR product was purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) and sequenced using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems, Foster City, CA, USA). .. Sequencing data were analyzed using SeqScape v2.5 software (Applied Biosystems, Foster City, CA, USA).

Article Title: Detection and molecular typing of Leishmania tropica from Phlebotomus sergenti and lesions of cutaneous leishmaniasis in an emerging focus of Morocco
Article Snippet: .. The final PCR products of 400 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. Sequencing data were analyzed using SeqScape v.2.5 software (Applied Biosystems).

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: .. The final PCR products of 359 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. Sequencing data were analyzed using SeqScape v.2.5 software (Applied Biosystems).

Incubation:

Article Title: Detection and molecular typing of Leishmania tropica from Phlebotomus sergenti and lesions of cutaneous leishmaniasis in an emerging focus of Morocco
Article Snippet: The mixture was incubated in thermocycler (S1000™ Thermal Cycler, Bio-Rad) under the following conditions: an initial denaturation at 94°C for 3 min, followed by 37 cycles each consisting of three steps: 30 s at 94°C (denaturation), 30 s at 58°C (annealing) and 90 s at 72°C (extension). .. The final PCR products of 400 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems).

Marker:

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: A 100 bp DNA ladder was used as the standard marker for comparison. .. The final PCR products of 359 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems).

Staining:

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: Amplified DNA products were confirmed with 1.2% agarose gel and visualized under ultraviolet (UV) light after staining with 2 mg/mL ethidium bromide. .. The final PCR products of 359 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems).

TaqMan SNP Genotyping Assay:

Article Title: Genetic Variation in the Interleukin-28B Gene Is Associated with Spontaneous Clearance and Progression of Hepatitis C Virus in Moroccan Patients
Article Snippet: The resulting 367-bp PCR product was purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) and sequenced using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems, Foster City, CA, USA). .. The SNP rs8099917 was genotyped using a predesigned TaqMan SNP Genotyping Assay (Applied Biosystems; assay ID C_11710096_10).

Nested PCR:

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: Paragraph title: Nested PCR for amplifying the ITS-5.8S rDNA gene of Leishmania species ... They were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using a BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems).

Article Title: Detection and molecular typing of Leishmania tropica from Phlebotomus sergenti and lesions of cutaneous leishmaniasis in an emerging focus of Morocco
Article Snippet: Paragraph title: Nested PCR for amplifying ITS1-5.8S rDNA gene of Leishmania species ... The final PCR products of 400 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems).

Software:

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: They were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using a BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. Sequencing data were analyzed using SeqScape v.2.5 software (Applied Biosystems).

Article Title: Genetic Variation in the Interleukin-28B Gene Is Associated with Spontaneous Clearance and Progression of Hepatitis C Virus in Moroccan Patients
Article Snippet: The genotype of each sample was attributed by the SDS 1.1 software for allelic discrimination (ABI7000, Applied Biosystems, Foster City, CA, USA). .. The resulting 367-bp PCR product was purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) and sequenced using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems, Foster City, CA, USA).

Article Title: Molecular identification of Leishmania infection in the most relevant sand fly species and in patient skin samples from a cutaneous leishmaniasis focus, in Morocco
Article Snippet: The obtained 350-bp ITS1-PCR products were purified using exonuclease I/shrimp alkaline phosphatase (GE Healthcare, US). .. Phylogenetic analysis was performed with MEGA version 7 software using the neighbour-joining and Kimura 2-parameter models.

Article Title: Molecular identification of Leishmania infection in the most relevant sand fly species and in patient skin samples from a cutaneous leishmaniasis focus, in Morocco
Article Snippet: Sequencing and phylogenetic analysis The obtained 350-bp ITS1-PCR products were purified using exonuclease I/shrimp alkaline phosphatase (GE Healthcare, US). .. Phylogenetic analysis was performed with MEGA version 7 software using the neighbour-joining and Kimura 2-parameter models.

Article Title: Detection and molecular typing of Leishmania tropica from Phlebotomus sergenti and lesions of cutaneous leishmaniasis in an emerging focus of Morocco
Article Snippet: The final PCR products of 400 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. Sequencing data were analyzed using SeqScape v.2.5 software (Applied Biosystems).

Article Title: Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco
Article Snippet: The final PCR products of 359 bp were purified using the Exonuclease I/Shrimp Alkaline Phosphatase (GE Healthcare, US) before sequencing by using BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and an ABI PRISM 3130 DNA automated sequencer (Applied Biosystems). .. Sequencing data were analyzed using SeqScape v.2.5 software (Applied Biosystems).

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    GE Healthcare exonuclease i shrimp alkaline phosphatase
    Exonuclease I Shrimp Alkaline Phosphatase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease i shrimp alkaline phosphatase/product/GE Healthcare
    Average 87 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    exonuclease i shrimp alkaline phosphatase - by Bioz Stars, 2020-01
    87/100 stars
      Buy from Supplier

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